quantitative research methodology sample

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A Practical Guide to Writing Quantitative and Qualitative Research Questions and Hypotheses in Scholarly Articles

Edward barroga.

1 Department of General Education, Graduate School of Nursing Science, St. Luke’s International University, Tokyo, Japan.

Glafera Janet Matanguihan

2 Department of Biological Sciences, Messiah University, Mechanicsburg, PA, USA.

The development of research questions and the subsequent hypotheses are prerequisites to defining the main research purpose and specific objectives of a study. Consequently, these objectives determine the study design and research outcome. The development of research questions is a process based on knowledge of current trends, cutting-edge studies, and technological advances in the research field. Excellent research questions are focused and require a comprehensive literature search and in-depth understanding of the problem being investigated. Initially, research questions may be written as descriptive questions which could be developed into inferential questions. These questions must be specific and concise to provide a clear foundation for developing hypotheses. Hypotheses are more formal predictions about the research outcomes. These specify the possible results that may or may not be expected regarding the relationship between groups. Thus, research questions and hypotheses clarify the main purpose and specific objectives of the study, which in turn dictate the design of the study, its direction, and outcome. Studies developed from good research questions and hypotheses will have trustworthy outcomes with wide-ranging social and health implications.

INTRODUCTION

Scientific research is usually initiated by posing evidenced-based research questions which are then explicitly restated as hypotheses. 1 , 2 The hypotheses provide directions to guide the study, solutions, explanations, and expected results. 3 , 4 Both research questions and hypotheses are essentially formulated based on conventional theories and real-world processes, which allow the inception of novel studies and the ethical testing of ideas. 5 , 6

It is crucial to have knowledge of both quantitative and qualitative research 2 as both types of research involve writing research questions and hypotheses. 7 However, these crucial elements of research are sometimes overlooked; if not overlooked, then framed without the forethought and meticulous attention it needs. Planning and careful consideration are needed when developing quantitative or qualitative research, particularly when conceptualizing research questions and hypotheses. 4

There is a continuing need to support researchers in the creation of innovative research questions and hypotheses, as well as for journal articles that carefully review these elements. 1 When research questions and hypotheses are not carefully thought of, unethical studies and poor outcomes usually ensue. Carefully formulated research questions and hypotheses define well-founded objectives, which in turn determine the appropriate design, course, and outcome of the study. This article then aims to discuss in detail the various aspects of crafting research questions and hypotheses, with the goal of guiding researchers as they develop their own. Examples from the authors and peer-reviewed scientific articles in the healthcare field are provided to illustrate key points.

DEFINITIONS AND RELATIONSHIP OF RESEARCH QUESTIONS AND HYPOTHESES

A research question is what a study aims to answer after data analysis and interpretation. The answer is written in length in the discussion section of the paper. Thus, the research question gives a preview of the different parts and variables of the study meant to address the problem posed in the research question. 1 An excellent research question clarifies the research writing while facilitating understanding of the research topic, objective, scope, and limitations of the study. 5

On the other hand, a research hypothesis is an educated statement of an expected outcome. This statement is based on background research and current knowledge. 8 , 9 The research hypothesis makes a specific prediction about a new phenomenon 10 or a formal statement on the expected relationship between an independent variable and a dependent variable. 3 , 11 It provides a tentative answer to the research question to be tested or explored. 4

Hypotheses employ reasoning to predict a theory-based outcome. 10 These can also be developed from theories by focusing on components of theories that have not yet been observed. 10 The validity of hypotheses is often based on the testability of the prediction made in a reproducible experiment. 8

Conversely, hypotheses can also be rephrased as research questions. Several hypotheses based on existing theories and knowledge may be needed to answer a research question. Developing ethical research questions and hypotheses creates a research design that has logical relationships among variables. These relationships serve as a solid foundation for the conduct of the study. 4 , 11 Haphazardly constructed research questions can result in poorly formulated hypotheses and improper study designs, leading to unreliable results. Thus, the formulations of relevant research questions and verifiable hypotheses are crucial when beginning research. 12

CHARACTERISTICS OF GOOD RESEARCH QUESTIONS AND HYPOTHESES

Excellent research questions are specific and focused. These integrate collective data and observations to confirm or refute the subsequent hypotheses. Well-constructed hypotheses are based on previous reports and verify the research context. These are realistic, in-depth, sufficiently complex, and reproducible. More importantly, these hypotheses can be addressed and tested. 13

There are several characteristics of well-developed hypotheses. Good hypotheses are 1) empirically testable 7 , 10 , 11 , 13 ; 2) backed by preliminary evidence 9 ; 3) testable by ethical research 7 , 9 ; 4) based on original ideas 9 ; 5) have evidenced-based logical reasoning 10 ; and 6) can be predicted. 11 Good hypotheses can infer ethical and positive implications, indicating the presence of a relationship or effect relevant to the research theme. 7 , 11 These are initially developed from a general theory and branch into specific hypotheses by deductive reasoning. In the absence of a theory to base the hypotheses, inductive reasoning based on specific observations or findings form more general hypotheses. 10

TYPES OF RESEARCH QUESTIONS AND HYPOTHESES

Research questions and hypotheses are developed according to the type of research, which can be broadly classified into quantitative and qualitative research. We provide a summary of the types of research questions and hypotheses under quantitative and qualitative research categories in Table 1 .

Research questions in quantitative research

In quantitative research, research questions inquire about the relationships among variables being investigated and are usually framed at the start of the study. These are precise and typically linked to the subject population, dependent and independent variables, and research design. 1 Research questions may also attempt to describe the behavior of a population in relation to one or more variables, or describe the characteristics of variables to be measured ( descriptive research questions ). 1 , 5 , 14 These questions may also aim to discover differences between groups within the context of an outcome variable ( comparative research questions ), 1 , 5 , 14 or elucidate trends and interactions among variables ( relationship research questions ). 1 , 5 We provide examples of descriptive, comparative, and relationship research questions in quantitative research in Table 2 .

Hypotheses in quantitative research

In quantitative research, hypotheses predict the expected relationships among variables. 15 Relationships among variables that can be predicted include 1) between a single dependent variable and a single independent variable ( simple hypothesis ) or 2) between two or more independent and dependent variables ( complex hypothesis ). 4 , 11 Hypotheses may also specify the expected direction to be followed and imply an intellectual commitment to a particular outcome ( directional hypothesis ) 4 . On the other hand, hypotheses may not predict the exact direction and are used in the absence of a theory, or when findings contradict previous studies ( non-directional hypothesis ). 4 In addition, hypotheses can 1) define interdependency between variables ( associative hypothesis ), 4 2) propose an effect on the dependent variable from manipulation of the independent variable ( causal hypothesis ), 4 3) state a negative relationship between two variables ( null hypothesis ), 4 , 11 , 15 4) replace the working hypothesis if rejected ( alternative hypothesis ), 15 explain the relationship of phenomena to possibly generate a theory ( working hypothesis ), 11 5) involve quantifiable variables that can be tested statistically ( statistical hypothesis ), 11 6) or express a relationship whose interlinks can be verified logically ( logical hypothesis ). 11 We provide examples of simple, complex, directional, non-directional, associative, causal, null, alternative, working, statistical, and logical hypotheses in quantitative research, as well as the definition of quantitative hypothesis-testing research in Table 3 .

Research questions in qualitative research

Unlike research questions in quantitative research, research questions in qualitative research are usually continuously reviewed and reformulated. The central question and associated subquestions are stated more than the hypotheses. 15 The central question broadly explores a complex set of factors surrounding the central phenomenon, aiming to present the varied perspectives of participants. 15

There are varied goals for which qualitative research questions are developed. These questions can function in several ways, such as to 1) identify and describe existing conditions ( contextual research question s); 2) describe a phenomenon ( descriptive research questions ); 3) assess the effectiveness of existing methods, protocols, theories, or procedures ( evaluation research questions ); 4) examine a phenomenon or analyze the reasons or relationships between subjects or phenomena ( explanatory research questions ); or 5) focus on unknown aspects of a particular topic ( exploratory research questions ). 5 In addition, some qualitative research questions provide new ideas for the development of theories and actions ( generative research questions ) or advance specific ideologies of a position ( ideological research questions ). 1 Other qualitative research questions may build on a body of existing literature and become working guidelines ( ethnographic research questions ). Research questions may also be broadly stated without specific reference to the existing literature or a typology of questions ( phenomenological research questions ), may be directed towards generating a theory of some process ( grounded theory questions ), or may address a description of the case and the emerging themes ( qualitative case study questions ). 15 We provide examples of contextual, descriptive, evaluation, explanatory, exploratory, generative, ideological, ethnographic, phenomenological, grounded theory, and qualitative case study research questions in qualitative research in Table 4 , and the definition of qualitative hypothesis-generating research in Table 5 .

Qualitative studies usually pose at least one central research question and several subquestions starting with How or What . These research questions use exploratory verbs such as explore or describe . These also focus on one central phenomenon of interest, and may mention the participants and research site. 15

Hypotheses in qualitative research

Hypotheses in qualitative research are stated in the form of a clear statement concerning the problem to be investigated. Unlike in quantitative research where hypotheses are usually developed to be tested, qualitative research can lead to both hypothesis-testing and hypothesis-generating outcomes. 2 When studies require both quantitative and qualitative research questions, this suggests an integrative process between both research methods wherein a single mixed-methods research question can be developed. 1

FRAMEWORKS FOR DEVELOPING RESEARCH QUESTIONS AND HYPOTHESES

Research questions followed by hypotheses should be developed before the start of the study. 1 , 12 , 14 It is crucial to develop feasible research questions on a topic that is interesting to both the researcher and the scientific community. This can be achieved by a meticulous review of previous and current studies to establish a novel topic. Specific areas are subsequently focused on to generate ethical research questions. The relevance of the research questions is evaluated in terms of clarity of the resulting data, specificity of the methodology, objectivity of the outcome, depth of the research, and impact of the study. 1 , 5 These aspects constitute the FINER criteria (i.e., Feasible, Interesting, Novel, Ethical, and Relevant). 1 Clarity and effectiveness are achieved if research questions meet the FINER criteria. In addition to the FINER criteria, Ratan et al. described focus, complexity, novelty, feasibility, and measurability for evaluating the effectiveness of research questions. 14

The PICOT and PEO frameworks are also used when developing research questions. 1 The following elements are addressed in these frameworks, PICOT: P-population/patients/problem, I-intervention or indicator being studied, C-comparison group, O-outcome of interest, and T-timeframe of the study; PEO: P-population being studied, E-exposure to preexisting conditions, and O-outcome of interest. 1 Research questions are also considered good if these meet the “FINERMAPS” framework: Feasible, Interesting, Novel, Ethical, Relevant, Manageable, Appropriate, Potential value/publishable, and Systematic. 14

As we indicated earlier, research questions and hypotheses that are not carefully formulated result in unethical studies or poor outcomes. To illustrate this, we provide some examples of ambiguous research question and hypotheses that result in unclear and weak research objectives in quantitative research ( Table 6 ) 16 and qualitative research ( Table 7 ) 17 , and how to transform these ambiguous research question(s) and hypothesis(es) into clear and good statements.

a These statements were composed for comparison and illustrative purposes only.

b These statements are direct quotes from Higashihara and Horiuchi. 16

a This statement is a direct quote from Shimoda et al. 17

The other statements were composed for comparison and illustrative purposes only.

CONSTRUCTING RESEARCH QUESTIONS AND HYPOTHESES

To construct effective research questions and hypotheses, it is very important to 1) clarify the background and 2) identify the research problem at the outset of the research, within a specific timeframe. 9 Then, 3) review or conduct preliminary research to collect all available knowledge about the possible research questions by studying theories and previous studies. 18 Afterwards, 4) construct research questions to investigate the research problem. Identify variables to be accessed from the research questions 4 and make operational definitions of constructs from the research problem and questions. Thereafter, 5) construct specific deductive or inductive predictions in the form of hypotheses. 4 Finally, 6) state the study aims . This general flow for constructing effective research questions and hypotheses prior to conducting research is shown in Fig. 1 .

Research questions are used more frequently in qualitative research than objectives or hypotheses. 3 These questions seek to discover, understand, explore or describe experiences by asking “What” or “How.” The questions are open-ended to elicit a description rather than to relate variables or compare groups. The questions are continually reviewed, reformulated, and changed during the qualitative study. 3 Research questions are also used more frequently in survey projects than hypotheses in experiments in quantitative research to compare variables and their relationships.

Hypotheses are constructed based on the variables identified and as an if-then statement, following the template, ‘If a specific action is taken, then a certain outcome is expected.’ At this stage, some ideas regarding expectations from the research to be conducted must be drawn. 18 Then, the variables to be manipulated (independent) and influenced (dependent) are defined. 4 Thereafter, the hypothesis is stated and refined, and reproducible data tailored to the hypothesis are identified, collected, and analyzed. 4 The hypotheses must be testable and specific, 18 and should describe the variables and their relationships, the specific group being studied, and the predicted research outcome. 18 Hypotheses construction involves a testable proposition to be deduced from theory, and independent and dependent variables to be separated and measured separately. 3 Therefore, good hypotheses must be based on good research questions constructed at the start of a study or trial. 12

In summary, research questions are constructed after establishing the background of the study. Hypotheses are then developed based on the research questions. Thus, it is crucial to have excellent research questions to generate superior hypotheses. In turn, these would determine the research objectives and the design of the study, and ultimately, the outcome of the research. 12 Algorithms for building research questions and hypotheses are shown in Fig. 2 for quantitative research and in Fig. 3 for qualitative research.

EXAMPLES OF RESEARCH QUESTIONS FROM PUBLISHED ARTICLES

• EXAMPLE 1. Descriptive research question (quantitative research)
• - Presents research variables to be assessed (distinct phenotypes and subphenotypes)
• “BACKGROUND: Since COVID-19 was identified, its clinical and biological heterogeneity has been recognized. Identifying COVID-19 phenotypes might help guide basic, clinical, and translational research efforts.
• RESEARCH QUESTION: Does the clinical spectrum of patients with COVID-19 contain distinct phenotypes and subphenotypes? ” 19
• EXAMPLE 2. Relationship research question (quantitative research)
• - Shows interactions between dependent variable (static postural control) and independent variable (peripheral visual field loss)
• “Background: Integration of visual, vestibular, and proprioceptive sensations contributes to postural control. People with peripheral visual field loss have serious postural instability. However, the directional specificity of postural stability and sensory reweighting caused by gradual peripheral visual field loss remain unclear.
• Research question: What are the effects of peripheral visual field loss on static postural control ?” 20
• EXAMPLE 3. Comparative research question (quantitative research)
• - Clarifies the difference among groups with an outcome variable (patients enrolled in COMPERA with moderate PH or severe PH in COPD) and another group without the outcome variable (patients with idiopathic pulmonary arterial hypertension (IPAH))
• “BACKGROUND: Pulmonary hypertension (PH) in COPD is a poorly investigated clinical condition.
• RESEARCH QUESTION: Which factors determine the outcome of PH in COPD?
• STUDY DESIGN AND METHODS: We analyzed the characteristics and outcome of patients enrolled in the Comparative, Prospective Registry of Newly Initiated Therapies for Pulmonary Hypertension (COMPERA) with moderate or severe PH in COPD as defined during the 6th PH World Symposium who received medical therapy for PH and compared them with patients with idiopathic pulmonary arterial hypertension (IPAH) .” 21
• EXAMPLE 4. Exploratory research question (qualitative research)
• - Explores areas that have not been fully investigated (perspectives of families and children who receive care in clinic-based child obesity treatment) to have a deeper understanding of the research problem
• “Problem: Interventions for children with obesity lead to only modest improvements in BMI and long-term outcomes, and data are limited on the perspectives of families of children with obesity in clinic-based treatment. This scoping review seeks to answer the question: What is known about the perspectives of families and children who receive care in clinic-based child obesity treatment? This review aims to explore the scope of perspectives reported by families of children with obesity who have received individualized outpatient clinic-based obesity treatment.” 22
• EXAMPLE 5. Relationship research question (quantitative research)
• - Defines interactions between dependent variable (use of ankle strategies) and independent variable (changes in muscle tone)
• “Background: To maintain an upright standing posture against external disturbances, the human body mainly employs two types of postural control strategies: “ankle strategy” and “hip strategy.” While it has been reported that the magnitude of the disturbance alters the use of postural control strategies, it has not been elucidated how the level of muscle tone, one of the crucial parameters of bodily function, determines the use of each strategy. We have previously confirmed using forward dynamics simulations of human musculoskeletal models that an increased muscle tone promotes the use of ankle strategies. The objective of the present study was to experimentally evaluate a hypothesis: an increased muscle tone promotes the use of ankle strategies. Research question: Do changes in the muscle tone affect the use of ankle strategies ?” 23

EXAMPLES OF HYPOTHESES IN PUBLISHED ARTICLES

• EXAMPLE 1. Working hypothesis (quantitative research)
• - A hypothesis that is initially accepted for further research to produce a feasible theory
• “As fever may have benefit in shortening the duration of viral illness, it is plausible to hypothesize that the antipyretic efficacy of ibuprofen may be hindering the benefits of a fever response when taken during the early stages of COVID-19 illness .” 24
• “In conclusion, it is plausible to hypothesize that the antipyretic efficacy of ibuprofen may be hindering the benefits of a fever response . The difference in perceived safety of these agents in COVID-19 illness could be related to the more potent efficacy to reduce fever with ibuprofen compared to acetaminophen. Compelling data on the benefit of fever warrant further research and review to determine when to treat or withhold ibuprofen for early stage fever for COVID-19 and other related viral illnesses .” 24
• EXAMPLE 2. Exploratory hypothesis (qualitative research)
• - Explores particular areas deeper to clarify subjective experience and develop a formal hypothesis potentially testable in a future quantitative approach
• “We hypothesized that when thinking about a past experience of help-seeking, a self distancing prompt would cause increased help-seeking intentions and more favorable help-seeking outcome expectations .” 25
• “Conclusion
• Although a priori hypotheses were not supported, further research is warranted as results indicate the potential for using self-distancing approaches to increasing help-seeking among some people with depressive symptomatology.” 25
• EXAMPLE 3. Hypothesis-generating research to establish a framework for hypothesis testing (qualitative research)
• “We hypothesize that compassionate care is beneficial for patients (better outcomes), healthcare systems and payers (lower costs), and healthcare providers (lower burnout). ” 26
• Compassionomics is the branch of knowledge and scientific study of the effects of compassionate healthcare. Our main hypotheses are that compassionate healthcare is beneficial for (1) patients, by improving clinical outcomes, (2) healthcare systems and payers, by supporting financial sustainability, and (3) HCPs, by lowering burnout and promoting resilience and well-being. The purpose of this paper is to establish a scientific framework for testing the hypotheses above . If these hypotheses are confirmed through rigorous research, compassionomics will belong in the science of evidence-based medicine, with major implications for all healthcare domains.” 26
• EXAMPLE 4. Statistical hypothesis (quantitative research)
• - An assumption is made about the relationship among several population characteristics ( gender differences in sociodemographic and clinical characteristics of adults with ADHD ). Validity is tested by statistical experiment or analysis ( chi-square test, Students t-test, and logistic regression analysis)
• “Our research investigated gender differences in sociodemographic and clinical characteristics of adults with ADHD in a Japanese clinical sample. Due to unique Japanese cultural ideals and expectations of women's behavior that are in opposition to ADHD symptoms, we hypothesized that women with ADHD experience more difficulties and present more dysfunctions than men . We tested the following hypotheses: first, women with ADHD have more comorbidities than men with ADHD; second, women with ADHD experience more social hardships than men, such as having less full-time employment and being more likely to be divorced.” 27
• “Statistical Analysis
• ( text omitted ) Between-gender comparisons were made using the chi-squared test for categorical variables and Students t-test for continuous variables…( text omitted ). A logistic regression analysis was performed for employment status, marital status, and comorbidity to evaluate the independent effects of gender on these dependent variables.” 27

EXAMPLES OF HYPOTHESIS AS WRITTEN IN PUBLISHED ARTICLES IN RELATION TO OTHER PARTS

• EXAMPLE 1. Background, hypotheses, and aims are provided
• “Pregnant women need skilled care during pregnancy and childbirth, but that skilled care is often delayed in some countries …( text omitted ). The focused antenatal care (FANC) model of WHO recommends that nurses provide information or counseling to all pregnant women …( text omitted ). Job aids are visual support materials that provide the right kind of information using graphics and words in a simple and yet effective manner. When nurses are not highly trained or have many work details to attend to, these job aids can serve as a content reminder for the nurses and can be used for educating their patients (Jennings, Yebadokpo, Affo, & Agbogbe, 2010) ( text omitted ). Importantly, additional evidence is needed to confirm how job aids can further improve the quality of ANC counseling by health workers in maternal care …( text omitted )” 28
• “ This has led us to hypothesize that the quality of ANC counseling would be better if supported by job aids. Consequently, a better quality of ANC counseling is expected to produce higher levels of awareness concerning the danger signs of pregnancy and a more favorable impression of the caring behavior of nurses .” 28
• “This study aimed to examine the differences in the responses of pregnant women to a job aid-supported intervention during ANC visit in terms of 1) their understanding of the danger signs of pregnancy and 2) their impression of the caring behaviors of nurses to pregnant women in rural Tanzania.” 28
• EXAMPLE 2. Background, hypotheses, and aims are provided
• “We conducted a two-arm randomized controlled trial (RCT) to evaluate and compare changes in salivary cortisol and oxytocin levels of first-time pregnant women between experimental and control groups. The women in the experimental group touched and held an infant for 30 min (experimental intervention protocol), whereas those in the control group watched a DVD movie of an infant (control intervention protocol). The primary outcome was salivary cortisol level and the secondary outcome was salivary oxytocin level.” 29
• “ We hypothesize that at 30 min after touching and holding an infant, the salivary cortisol level will significantly decrease and the salivary oxytocin level will increase in the experimental group compared with the control group .” 29
• EXAMPLE 3. Background, aim, and hypothesis are provided
• “In countries where the maternal mortality ratio remains high, antenatal education to increase Birth Preparedness and Complication Readiness (BPCR) is considered one of the top priorities [1]. BPCR includes birth plans during the antenatal period, such as the birthplace, birth attendant, transportation, health facility for complications, expenses, and birth materials, as well as family coordination to achieve such birth plans. In Tanzania, although increasing, only about half of all pregnant women attend an antenatal clinic more than four times [4]. Moreover, the information provided during antenatal care (ANC) is insufficient. In the resource-poor settings, antenatal group education is a potential approach because of the limited time for individual counseling at antenatal clinics.” 30
• “This study aimed to evaluate an antenatal group education program among pregnant women and their families with respect to birth-preparedness and maternal and infant outcomes in rural villages of Tanzania.” 30
• “ The study hypothesis was if Tanzanian pregnant women and their families received a family-oriented antenatal group education, they would (1) have a higher level of BPCR, (2) attend antenatal clinic four or more times, (3) give birth in a health facility, (4) have less complications of women at birth, and (5) have less complications and deaths of infants than those who did not receive the education .” 30

Research questions and hypotheses are crucial components to any type of research, whether quantitative or qualitative. These questions should be developed at the very beginning of the study. Excellent research questions lead to superior hypotheses, which, like a compass, set the direction of research, and can often determine the successful conduct of the study. Many research studies have floundered because the development of research questions and subsequent hypotheses was not given the thought and meticulous attention needed. The development of research questions and hypotheses is an iterative process based on extensive knowledge of the literature and insightful grasp of the knowledge gap. Focused, concise, and specific research questions provide a strong foundation for constructing hypotheses which serve as formal predictions about the research outcomes. Research questions and hypotheses are crucial elements of research that should not be overlooked. They should be carefully thought of and constructed when planning research. This avoids unethical studies and poor outcomes by defining well-founded objectives that determine the design, course, and outcome of the study.

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

• Conceptualization: Barroga E, Matanguihan GJ.
• Methodology: Barroga E, Matanguihan GJ.
• Writing - original draft: Barroga E, Matanguihan GJ.
• Writing - review & editing: Barroga E, Matanguihan GJ.

Research Methodologies

Quantitative research methodologies.

• Qualitative Research Methodologies
• Systematic Reviews
• Finding Articles by Methodology
• Design Your Research Project

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What is quantitative research.

Quantitative methodologies use statistics to analyze numerical data gathered by researchers to answer their research questions. Quantitative methods can be used to answer questions such as:

• What are the relationships between two or more variables? 
• What factors are at play in an environment that might affect the behavior or development of the organisms in that environment?

Quantitative methods can also be used to test hypotheses by conducting quasi-experimental studies or designing experiments.

Independent and Dependent Variables

In quantitative research, a variable is something (an intervention technique, a pharmaceutical, a temperature, etc.) that changes. There are two kinds of variables:  independent variables and dependent variables . In the simplest terms, the independent variable is whatever the researchers are using to attempt to make a change in their dependent variable.

* This is a real, repeatable experiment you can try on your plants.

Correlational

Researchers will compare two sets of numbers to try and identify a relationship (if any) between two things.

• Köse S., & Murat, M. (2021). Examination of the relationship between smartphone addiction and cyberchondria in adolescents. Archives of Psychiatric Nursing, 35(6): 563-570.
• Pilger et al. (2021). Spiritual well-being, religious/spiritual coping and quality of life among the elderly undergoing hemodialysis: a correlational study. Journal of Religion, Spirituality & Aging, 33(1): 2-15.

Descriptive

Researchers will attempt to quantify a variety of factors at play as they study a particular type of phenomenon or action. For example, researchers might use a descriptive methodology to understand the effects of climate change on the life cycle of a plant or animal. 

• Lakshmi, E. (2021). Food consumption pattern and body mass index of adolescents: A descriptive study. International Journal of Nutrition, Pharmacology, Neurological Diseases, 11(4), 293–297.
• Lin, J., Singh, S., Sha, L., Tan, W., Lang, D., Gašević, D., & Chen, G. (2022). Is it a good move? Mining effective tutoring strategies from human–human tutorial dialogues. Future Generation Computer Systems, 127, 194–207.

Experimental

To understand the effects of a variable, researchers will design an experiment where they can control as many factors as possible. This can involve creating control and experimental groups. The experimental group will be exposed to the variable to study its effects. The control group provides data about what happens when the variable is absent. For example, in a study about online teaching, the control group might receive traditional face-to-face instruction while the experimental group would receive their instruction virtually. 

• Jinzhang Jia, Yinuo Chen, Guangbo Che, Jinchao Zhu, Fengxiao Wang, & Peng Jia. (2021). Experimental study on the explosion characteristics of hydrogen-methane premixed gas in complex pipe networks. Scientific Reports, 11(1), 1–11.
• Sasaki, R. et al. (2021). Effects of cryotherapy applied at different temperatures on inflammatory pain during the acute phase of arthritis in rats. Physical Therapy, 101(2), 1–9.

Quasi-Experimental/Quasi-Comparative

Researchers will attempt to determine what (if any) effect a variable can have. These studies may have multiple independent variables (causes) and multiple dependent variables (effects), but this can complicate researchers' efforts to find out if A can cause B or if X, Y,  and  Z are also playing a role.

• Jafari, A., Alami, A., Charoghchian, E., Delshad Noghabi, A., & Nejatian, M. (2021). The impact of effective communication skills training on the status of marital burnout among married women. BMC Women’s Health, 21(1), 1-10.
• Phillips, S. W., Kim, D.-Y., Sobol, J. J., & Gayadeen, S. M. (2021). Total recall?: A quasi-experimental study of officer’s recollection in shoot - don’t shoot simulators. Police Practice and Research, 22(3), 1229–1240.

Surveys can be considered a quantitative methodology if the researchers require their respondents to choose from pre-determined responses. 

• Harries et al. (2021). Effects of the COVID-19 pandemic on medical students: A multicenter quantitative study. BMC Medical Education, 21(14), 1-8.
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Quantitative Research: What It Is, Practices & Methods

Quantitative research involves analyzing and gathering numerical data to uncover trends, calculate averages, evaluate relationships, and derive overarching insights. It’s used in various fields, including the natural and social sciences. Quantitative data analysis employs statistical techniques for processing and interpreting numeric data.

Research designs in the quantitative realm outline how data will be collected and analyzed with methods like experiments and surveys. Qualitative methods complement quantitative research by focusing on non-numerical data, adding depth to understanding. Data collection methods can be qualitative or quantitative, depending on research goals. Researchers often use a combination of both approaches to gain a comprehensive understanding of phenomena.

What is Quantitative Research?

Quantitative research is a systematic investigation of phenomena by gathering quantifiable data and performing statistical, mathematical, or computational techniques. Quantitative research collects statistically significant information from existing and potential customers using sampling methods and sending out online surveys , online polls , and questionnaires , for example.

One of the main characteristics of this type of research is that the results can be depicted in numerical form. After carefully collecting structured observations and understanding these numbers, it’s possible to predict the future of a product or service, establish causal relationships or Causal Research , and make changes accordingly. Quantitative research primarily centers on the analysis of numerical data and utilizes inferential statistics to derive conclusions that can be extrapolated to the broader population.

An example of a quantitative research study is the survey conducted to understand how long a doctor takes to tend to a patient when the patient walks into the hospital. A patient satisfaction survey can be administered to ask questions like how long a doctor takes to see a patient, how often a patient walks into a hospital, and other such questions, which are dependent variables in the research. This kind of research method is often employed in the social sciences, and it involves using mathematical frameworks and theories to effectively present data, ensuring that the results are logical, statistically sound, and unbiased.

Data collection in quantitative research uses a structured method and is typically conducted on larger samples representing the entire population. Researchers use quantitative methods to collect numerical data, which is then subjected to statistical analysis to determine statistically significant findings. This approach is valuable in both experimental research and social research, as it helps in making informed decisions and drawing reliable conclusions based on quantitative data.

Quantitative Research Characteristics

Quantitative research has several unique characteristics that make it well-suited for specific projects. Let’s explore the most crucial of these characteristics so that you can consider them when planning your next research project:

• Structured tools: Quantitative research relies on structured tools such as surveys, polls, or questionnaires to gather quantitative data . Using such structured methods helps collect in-depth and actionable numerical data from the survey respondents, making it easier to perform data analysis.
• Sample size: Quantitative research is conducted on a significant sample size  representing the target market . Appropriate Survey Sampling methods, a fundamental aspect of quantitative research methods, must be employed when deriving the sample to fortify the research objective and ensure the reliability of the results.
• Close-ended questions: Closed-ended questions , specifically designed to align with the research objectives, are a cornerstone of quantitative research. These questions facilitate the collection of quantitative data and are extensively used in data collection processes.
• Prior studies: Before collecting feedback from respondents, researchers often delve into previous studies related to the research topic. This preliminary research helps frame the study effectively and ensures the data collection process is well-informed.
• Quantitative data: Typically, quantitative data is represented using tables, charts, graphs, or other numerical forms. This visual representation aids in understanding the collected data and is essential for rigorous data analysis, a key component of quantitative research methods.
• Generalization of results: One of the strengths of quantitative research is its ability to generalize results to the entire population. It means that the findings derived from a sample can be extrapolated to make informed decisions and take appropriate actions for improvement based on numerical data analysis.

Quantitative Research Methods

Quantitative research methods are systematic approaches used to gather and analyze numerical data to understand and draw conclusions about a phenomenon or population. Here are the quantitative research methods:

• Primary quantitative research methods
• Secondary quantitative research methods

Primary Quantitative Research Methods

Primary quantitative research is the most widely used method of conducting market research. The distinct feature of primary research is that the researcher focuses on collecting data directly rather than depending on data collected from previously done research. Primary quantitative research design can be broken down into three further distinctive tracks and the process flow. They are:

A. Techniques and Types of Studies

There are multiple types of primary quantitative research. They can be distinguished into the four following distinctive methods, which are:

01. Survey Research

Survey Research is fundamental for all quantitative outcome research methodologies and studies. Surveys are used to ask questions to a sample of respondents, using various types such as online polls, online surveys, paper questionnaires, web-intercept surveys , etc. Every small and big organization intends to understand what their customers think about their products and services, how well new features are faring in the market, and other such details.

By conducting survey research, an organization can ask multiple survey questions , collect data from a pool of customers, and analyze this collected data to produce numerical results. It is the first step towards collecting data for any research. You can use single ease questions . A single-ease question is a straightforward query that elicits a concise and uncomplicated response.

This type of research can be conducted with a specific target audience group and also can be conducted across multiple groups along with comparative analysis . A prerequisite for this type of research is that the sample of respondents must have randomly selected members. This way, a researcher can easily maintain the accuracy of the obtained results as a huge variety of respondents will be addressed using random selection. 

Traditionally, survey research was conducted face-to-face or via phone calls. Still, with the progress made by online mediums such as email or social media, survey research has also spread to online mediums.There are two types of surveys , either of which can be chosen based on the time in hand and the kind of data required:

Cross-sectional surveys: Cross-sectional surveys are observational surveys conducted in situations where the researcher intends to collect data from a sample of the target population at a given point in time. Researchers can evaluate various variables at a particular time. Data gathered using this type of survey is from people who depict similarity in all variables except the variables which are considered for research . Throughout the survey, this one variable will stay constant.

• Cross-sectional surveys are popular with retail, SMEs, and healthcare industries. Information is garnered without modifying any parameters in the variable ecosystem.
• Multiple samples can be analyzed and compared using a cross-sectional survey research method.
• Multiple variables can be evaluated using this type of survey research.
• The only disadvantage of cross-sectional surveys is that the cause-effect relationship of variables cannot be established as it usually evaluates variables at a particular time and not across a continuous time frame.

Longitudinal surveys: Longitudinal surveys are also observational surveys , but unlike cross-sectional surveys, longitudinal surveys are conducted across various time durations to observe a change in respondent behavior and thought processes. This time can be days, months, years, or even decades. For instance, a researcher planning to analyze the change in buying habits of teenagers over 5 years will conduct longitudinal surveys.

• In cross-sectional surveys, the same variables were evaluated at a given time, and in longitudinal surveys, different variables can be analyzed at different intervals.
• Longitudinal surveys are extensively used in the field of medicine and applied sciences. Apart from these two fields, they are also used to observe a change in the market trend analysis , analyze customer satisfaction, or gain feedback on products/services.
• In situations where the sequence of events is highly essential, longitudinal surveys are used.
• Researchers say that when research subjects need to be thoroughly inspected before concluding, they rely on longitudinal surveys.

02. Correlational Research

A comparison between two entities is invariable. Correlation research is conducted to establish a relationship between two closely-knit entities and how one impacts the other, and what changes are eventually observed. This research method is carried out to give value to naturally occurring relationships, and a minimum of two different groups are required to conduct this quantitative research method successfully. Without assuming various aspects, a relationship between two groups or entities must be established.

Researchers use this quantitative research design to correlate two or more variables using mathematical analysis methods. Patterns, relationships, and trends between variables are concluded as they exist in their original setup. The impact of one of these variables on the other is observed, along with how it changes the relationship between the two variables. Researchers tend to manipulate one of the variables to attain the desired results.

Ideally, it is advised not to make conclusions merely based on correlational research. This is because it is not mandatory that if two variables are in sync that they are interrelated.

Example of Correlational Research Questions :

• The relationship between stress and depression.
• The equation between fame and money.
• The relation between activities in a third-grade class and its students.

03. Causal-comparative Research

This research method mainly depends on the factor of comparison. Also called quasi-experimental research , this quantitative research method is used by researchers to conclude the cause-effect equation between two or more variables, where one variable is dependent on the other independent variable. The independent variable is established but not manipulated, and its impact on the dependent variable is observed. These variables or groups must be formed as they exist in the natural setup. As the dependent and independent variables will always exist in a group, it is advised that the conclusions are carefully established by keeping all the factors in mind.

Causal-comparative research is not restricted to the statistical analysis of two variables but extends to analyzing how various variables or groups change under the influence of the same changes. This research is conducted irrespective of the type of relationship that exists between two or more variables. Statistical analysis plan is used to present the outcome using this quantitative research method.

Example of Causal-Comparative Research Questions:

• The impact of drugs on a teenager. The effect of good education on a freshman. The effect of substantial food provision in the villages of Africa.

04. Experimental Research

Also known as true experimentation, this research method relies on a theory. As the name suggests, experimental research is usually based on one or more theories. This theory has yet to be proven before and is merely a supposition. In experimental research, an analysis is done around proving or disproving the statement. This research method is used in natural sciences. Traditional research methods are more effective than modern techniques.

There can be multiple theories in experimental research. A theory is a statement that can be verified or refuted.

After establishing the statement, efforts are made to understand whether it is valid or invalid. This quantitative research method is mainly used in natural or social sciences as various statements must be proved right or wrong.

• Traditional research methods are more effective than modern techniques.
• Systematic teaching schedules help children who struggle to cope with the course.
• It is a boon to have responsible nursing staff for ailing parents.

B. Data Collection Methodologies

The second major step in primary quantitative research is data collection. Data collection can be divided into sampling methods and data collection using surveys and polls.

01. Data Collection Methodologies: Sampling Methods

There are two main sampling methods for quantitative research: Probability and Non-probability sampling .

Probability sampling: A theory of probability is used to filter individuals from a population and create samples in probability sampling . Participants of a sample are chosen by random selection processes. Each target audience member has an equal opportunity to be selected in the sample.

There are four main types of probability sampling:

• Simple random sampling: As the name indicates, simple random sampling is nothing but a random selection of elements for a sample. This sampling technique is implemented where the target population is considerably large.
• Stratified random sampling: In the stratified random sampling method , a large population is divided into groups (strata), and members of a sample are chosen randomly from these strata. The various segregated strata should ideally not overlap one another.
• Cluster sampling: Cluster sampling is a probability sampling method using which the main segment is divided into clusters, usually using geographic segmentation and demographic segmentation parameters.
• Systematic sampling: Systematic sampling is a technique where the starting point of the sample is chosen randomly, and all the other elements are chosen using a fixed interval. This interval is calculated by dividing the population size by the target sample size.

Non-probability sampling: Non-probability sampling is where the researcher’s knowledge and experience are used to create samples. Because of the researcher’s involvement, not all the target population members have an equal probability of being selected to be a part of a sample.

There are five non-probability sampling models:

• Convenience sampling: In convenience sampling , elements of a sample are chosen only due to one prime reason: their proximity to the researcher. These samples are quick and easy to implement as there is no other parameter of selection involved.
• Consecutive sampling: Consecutive sampling is quite similar to convenience sampling, except for the fact that researchers can choose a single element or a group of samples and conduct research consecutively over a significant period and then perform the same process with other samples.
• Quota sampling: Using quota sampling , researchers can select elements using their knowledge of target traits and personalities to form strata. Members of various strata can then be chosen to be a part of the sample as per the researcher’s understanding.
• Snowball sampling: Snowball sampling is conducted with target audiences who are difficult to contact and get information. It is popular in cases where the target audience for analysis research is rare to put together.
• Judgmental sampling: Judgmental sampling is a non-probability sampling method where samples are created only based on the researcher’s experience and research skill .

02. Data collection methodologies: Using surveys & polls

Once the sample is determined, then either surveys or polls can be distributed to collect the data for quantitative research.

Using surveys for primary quantitative research

A survey is defined as a research method used for collecting data from a pre-defined group of respondents to gain information and insights on various topics of interest. The ease of survey distribution and the wide number of people it can reach depending on the research time and objective makes it one of the most important aspects of conducting quantitative research.

Fundamental levels of measurement – nominal, ordinal, interval, and ratio scales

Four measurement scales are fundamental to creating a multiple-choice question in a survey. They are nominal, ordinal, interval, and ratio measurement scales without the fundamentals of which no multiple-choice questions can be created. Hence, it is crucial to understand these measurement levels to develop a robust survey.

Use of different question types

To conduct quantitative research, close-ended questions must be used in a survey. They can be a mix of multiple question types, including multiple-choice questions like semantic differential scale questions , rating scale questions , etc.

Survey Distribution and Survey Data Collection

In the above, we have seen the process of building a survey along with the research design to conduct primary quantitative research. Survey distribution to collect data is the other important aspect of the survey process. There are different ways of survey distribution. Some of the most commonly used methods are:

• Email: Sending a survey via email is the most widely used and effective survey distribution method. This method’s response rate is high because the respondents know your brand. You can use the QuestionPro email management feature to send out and collect survey responses.
• Buy respondents: Another effective way to distribute a survey and conduct primary quantitative research is to use a sample. Since the respondents are knowledgeable and are on the panel by their own will, responses are much higher.
• Embed survey on a website: Embedding a survey on a website increases a high number of responses as the respondent is already in close proximity to the brand when the survey pops up.
• Social distribution: Using social media to distribute the survey aids in collecting a higher number of responses from the people that are aware of the brand.
• QR code: QuestionPro QR codes store the URL for the survey. You can print/publish this code in magazines, signs, business cards, or on just about any object/medium.
• SMS survey: The SMS survey is a quick and time-effective way to collect a high number of responses.
• Offline Survey App: The QuestionPro App allows users to circulate surveys quickly, and the responses can be collected both online and offline.

Survey example

An example of a survey is a short customer satisfaction (CSAT) survey that can quickly be built and deployed to collect feedback about what the customer thinks about a brand and how satisfied and referenceable the brand is.

Using polls for primary quantitative research

Polls are a method to collect feedback using close-ended questions from a sample. The most commonly used types of polls are election polls and exit polls . Both of these are used to collect data from a large sample size but using basic question types like multiple-choice questions.

C. Data Analysis Techniques

The third aspect of primary quantitative research design is data analysis . After collecting raw data, there must be an analysis of this data to derive statistical inferences from this research. It is important to relate the results to the research objective and establish the statistical relevance of the results.

Remember to consider aspects of research that were not considered for the data collection process and report the difference between what was planned vs. what was actually executed.

It is then required to select precise Statistical Analysis Methods , such as SWOT, Conjoint, Cross-tabulation, etc., to analyze the quantitative data.

• SWOT analysis: SWOT Analysis stands for the acronym of Strengths, Weaknesses, Opportunities, and Threat analysis. Organizations use this statistical analysis technique to evaluate their performance internally and externally to develop effective strategies for improvement.
• Conjoint Analysis: Conjoint Analysis is a market analysis method to learn how individuals make complicated purchasing decisions. Trade-offs are involved in an individual’s daily activities, and these reflect their ability to decide from a complex list of product/service options.
• Cross-tabulation: Cross-tabulation is one of the preliminary statistical market analysis methods which establishes relationships, patterns, and trends within the various parameters of the research study.
• TURF Analysis: TURF Analysis , an acronym for Totally Unduplicated Reach and Frequency Analysis, is executed in situations where the reach of a favorable communication source is to be analyzed along with the frequency of this communication. It is used for understanding the potential of a target market.

Inferential statistics methods such as confidence interval, the margin of error, etc., can then be used to provide results.

Secondary Quantitative Research Methods

Secondary quantitative research or desk research is a research method that involves using already existing data or secondary data. Existing data is summarized and collated to increase the overall effectiveness of the research.

This research method involves collecting quantitative data from existing data sources like the internet, government resources, libraries, research reports, etc. Secondary quantitative research helps to validate the data collected from primary quantitative research and aid in strengthening or proving, or disproving previously collected data.

The following are five popularly used secondary quantitative research methods:

• Data available on the internet: With the high penetration of the internet and mobile devices, it has become increasingly easy to conduct quantitative research using the internet. Information about most research topics is available online, and this aids in boosting the validity of primary quantitative data.
• Government and non-government sources: Secondary quantitative research can also be conducted with the help of government and non-government sources that deal with market research reports. This data is highly reliable and in-depth and hence, can be used to increase the validity of quantitative research design.
• Public libraries: Now a sparingly used method of conducting quantitative research, it is still a reliable source of information, though. Public libraries have copies of important research that was conducted earlier. They are a storehouse of valuable information and documents from which information can be extracted.
• Educational institutions: Educational institutions conduct in-depth research on multiple topics, and hence, the reports that they publish are an important source of validation in quantitative research.
• Commercial information sources: Local newspapers, journals, magazines, radio, and TV stations are great sources to obtain data for secondary quantitative research. These commercial information sources have in-depth, first-hand information on market research, demographic segmentation, and similar subjects.

Quantitative Research Examples

Some examples of quantitative research are:

• A customer satisfaction template can be used if any organization would like to conduct a customer satisfaction (CSAT) survey . Through this kind of survey, an organization can collect quantitative data and metrics on the goodwill of the brand or organization in the customer’s mind based on multiple parameters such as product quality, pricing, customer experience, etc. This data can be collected by asking a net promoter score (NPS) question , matrix table questions, etc. that provide data in the form of numbers that can be analyzed and worked upon.
• Another example of quantitative research is an organization that conducts an event, collecting feedback from attendees about the value they see from the event. By using an event survey , the organization can collect actionable feedback about the satisfaction levels of customers during various phases of the event such as the sales, pre and post-event, the likelihood of recommending the organization to their friends and colleagues, hotel preferences for the future events and other such questions.

What are the Advantages of Quantitative Research?

There are many advantages to quantitative research. Some of the major advantages of why researchers use this method in market research are:

Collect Reliable and Accurate Data:

Quantitative research is a powerful method for collecting reliable and accurate quantitative data. Since data is collected, analyzed, and presented in numbers, the results obtained are incredibly reliable and objective. Numbers do not lie and offer an honest and precise picture of the conducted research without discrepancies. In situations where a researcher aims to eliminate bias and predict potential conflicts, quantitative research is the method of choice.

Quick Data Collection:

Quantitative research involves studying a group of people representing a larger population. Researchers use a survey or another quantitative research method to efficiently gather information from these participants, making the process of analyzing the data and identifying patterns faster and more manageable through the use of statistical analysis. This advantage makes quantitative research an attractive option for projects with time constraints.

Wider Scope of Data Analysis:

Quantitative research, thanks to its utilization of statistical methods, offers an extensive range of data collection and analysis. Researchers can delve into a broader spectrum of variables and relationships within the data, enabling a more thorough comprehension of the subject under investigation. This expanded scope is precious when dealing with complex research questions that require in-depth numerical analysis.

Eliminate Bias:

One of the significant advantages of quantitative research is its ability to eliminate bias. This research method leaves no room for personal comments or the biasing of results, as the findings are presented in numerical form. This objectivity makes the results fair and reliable in most cases, reducing the potential for researcher bias or subjectivity.

In summary, quantitative research involves collecting, analyzing, and presenting quantitative data using statistical analysis. It offers numerous advantages, including the collection of reliable and accurate data, quick data collection, a broader scope of data analysis, and the elimination of bias, making it a valuable approach in the field of research. When considering the benefits of quantitative research, it’s essential to recognize its strengths in contrast to qualitative methods and its role in collecting and analyzing numerical data for a more comprehensive understanding of research topics.

Best Practices to Conduct Quantitative Research

Here are some best practices for conducting quantitative research:

• Differentiate between quantitative and qualitative: Understand the difference between the two methodologies and apply the one that suits your needs best.
• Choose a suitable sample size: Ensure that you have a sample representative of your population and large enough to be statistically weighty.
• Keep your research goals clear and concise: Know your research goals before you begin data collection to ensure you collect the right amount and the right quantity of data.
• Keep the questions simple: Remember that you will be reaching out to a demographically wide audience. Pose simple questions for your respondents to understand easily.

Quantitative Research vs Qualitative Research

Quantitative research and qualitative research are two distinct approaches to conducting research, each with its own set of methods and objectives. Here’s a comparison of the two:

Quantitative Research

• Objective: The primary goal of quantitative research is to quantify and measure phenomena by collecting numerical data. It aims to test hypotheses, establish patterns, and generalize findings to a larger population.
• Data Collection: Quantitative research employs systematic and standardized approaches for data collection, including techniques like surveys, experiments, and observations that involve predefined variables. It is often collected from a large and representative sample.
• Data Analysis: Data is analyzed using statistical techniques, such as descriptive statistics, inferential statistics, and mathematical modeling. Researchers use statistical tests to draw conclusions and make generalizations based on numerical data.
• Sample Size: Quantitative research often involves larger sample sizes to ensure statistical significance and generalizability.
• Results: The results are typically presented in tables, charts, and statistical summaries, making them highly structured and objective.
• Generalizability: Researchers intentionally structure quantitative research to generate outcomes that can be helpful to a larger population, and they frequently seek to establish causative connections.
• Emphasis on Objectivity: Researchers aim to minimize bias and subjectivity, focusing on replicable and objective findings.

Qualitative Research

• Objective: Qualitative research seeks to gain a deeper understanding of the underlying motivations, behaviors, and experiences of individuals or groups. It explores the context and meaning of phenomena.
• Data Collection: Qualitative research employs adaptable and open-ended techniques for data collection, including methods like interviews, focus groups, observations, and content analysis. It allows participants to express their perspectives in their own words.
• Data Analysis: Data is analyzed through thematic analysis, content analysis, or grounded theory. Researchers focus on identifying patterns, themes, and insights in the data.
• Sample Size: Qualitative research typically involves smaller sample sizes due to the in-depth nature of data collection and analysis.
• Results: Findings are presented in narrative form, often in the participants’ own words. Results are subjective, context-dependent, and provide rich, detailed descriptions.
• Generalizability: Qualitative research does not aim for broad generalizability but focuses on in-depth exploration within a specific context. It provides a detailed understanding of a particular group or situation.
• Emphasis on Subjectivity: Researchers acknowledge the role of subjectivity and the researcher’s influence on the Research Process . Participant perspectives and experiences are central to the findings.

Researchers choose between quantitative and qualitative research methods based on their research objectives and the nature of the research question. Each approach has its advantages and drawbacks, and the decision between them hinges on the particular research objectives and the data needed to address research inquiries effectively.

Quantitative research is a structured way of collecting and analyzing data from various sources. Its purpose is to quantify the problem and understand its extent, seeking results that someone can project to a larger population.

Companies that use quantitative rather than qualitative research typically aim to measure magnitudes and seek objectively interpreted statistical results. So if you want to obtain quantitative data that helps you define the structured cause-and-effect relationship between the research problem and the factors, you should opt for this type of research.

At QuestionPro , we have various Best Data Collection Tools and features to conduct investigations of this type. You can create questionnaires and distribute them through our various methods. We also have sample services or various questions to guarantee the success of your study and the quality of the collected data.

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Quantitative research is a systematic and structured approach to studying phenomena that involves the collection of measurable data and the application of statistical, mathematical, or computational techniques for analysis.

Quantitative research is characterized by structured tools like surveys, substantial sample sizes, closed-ended questions, reliance on prior studies, data presented numerically, and the ability to generalize findings to the broader population.

The two main methods of quantitative research are Primary quantitative research methods, involving data collection directly from sources, and Secondary quantitative research methods, which utilize existing data for analysis.

1.Surveying to measure employee engagement with numerical rating scales. 2.Analyzing sales data to identify trends in product demand and market share. 4.Examining test scores to assess the impact of a new teaching method on student performance. 4.Using website analytics to track user behavior and conversion rates for an online store.

1.Differentiate between quantitative and qualitative approaches. 2.Choose a representative sample size. 3.Define clear research goals before data collection. 4.Use simple and easily understandable survey questions.

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Quantitative methods emphasize objective measurements and the statistical, mathematical, or numerical analysis of data collected through polls, questionnaires, and surveys, or by manipulating pre-existing statistical data using computational techniques . Quantitative research focuses on gathering numerical data and generalizing it across groups of people or to explain a particular phenomenon.

Babbie, Earl R. The Practice of Social Research . 12th ed. Belmont, CA: Wadsworth Cengage, 2010; Muijs, Daniel. Doing Quantitative Research in Education with SPSS . 2nd edition. London: SAGE Publications, 2010.

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Characteristics of Quantitative Research

Your goal in conducting quantitative research study is to determine the relationship between one thing [an independent variable] and another [a dependent or outcome variable] within a population. Quantitative research designs are either descriptive [subjects usually measured once] or experimental [subjects measured before and after a treatment]. A descriptive study establishes only associations between variables; an experimental study establishes causality.

Quantitative research deals in numbers, logic, and an objective stance. Quantitative research focuses on numeric and unchanging data and detailed, convergent reasoning rather than divergent reasoning [i.e., the generation of a variety of ideas about a research problem in a spontaneous, free-flowing manner].

Its main characteristics are :

• The data is usually gathered using structured research instruments.
• The results are based on larger sample sizes that are representative of the population.
• The research study can usually be replicated or repeated, given its high reliability.
• Researcher has a clearly defined research question to which objective answers are sought.
• All aspects of the study are carefully designed before data is collected.
• Data are in the form of numbers and statistics, often arranged in tables, charts, figures, or other non-textual forms.
• Project can be used to generalize concepts more widely, predict future results, or investigate causal relationships.
• Researcher uses tools, such as questionnaires or computer software, to collect numerical data.

The overarching aim of a quantitative research study is to classify features, count them, and construct statistical models in an attempt to explain what is observed.

  Things to keep in mind when reporting the results of a study using quantitative methods :

• Explain the data collected and their statistical treatment as well as all relevant results in relation to the research problem you are investigating. Interpretation of results is not appropriate in this section.
• Report unanticipated events that occurred during your data collection. Explain how the actual analysis differs from the planned analysis. Explain your handling of missing data and why any missing data does not undermine the validity of your analysis.
• Explain the techniques you used to "clean" your data set.
• Choose a minimally sufficient statistical procedure ; provide a rationale for its use and a reference for it. Specify any computer programs used.
• Describe the assumptions for each procedure and the steps you took to ensure that they were not violated.
• When using inferential statistics , provide the descriptive statistics, confidence intervals, and sample sizes for each variable as well as the value of the test statistic, its direction, the degrees of freedom, and the significance level [report the actual p value].
• Avoid inferring causality , particularly in nonrandomized designs or without further experimentation.
• Use tables to provide exact values ; use figures to convey global effects. Keep figures small in size; include graphic representations of confidence intervals whenever possible.
• Always tell the reader what to look for in tables and figures .

NOTE:   When using pre-existing statistical data gathered and made available by anyone other than yourself [e.g., government agency], you still must report on the methods that were used to gather the data and describe any missing data that exists and, if there is any, provide a clear explanation why the missing data does not undermine the validity of your final analysis.

Babbie, Earl R. The Practice of Social Research . 12th ed. Belmont, CA: Wadsworth Cengage, 2010; Brians, Craig Leonard et al. Empirical Political Analysis: Quantitative and Qualitative Research Methods . 8th ed. Boston, MA: Longman, 2011; McNabb, David E. Research Methods in Public Administration and Nonprofit Management: Quantitative and Qualitative Approaches . 2nd ed. Armonk, NY: M.E. Sharpe, 2008; Quantitative Research Methods. Writing@CSU. Colorado State University; Singh, Kultar. Quantitative Social Research Methods . Los Angeles, CA: Sage, 2007.

Basic Research Design for Quantitative Studies

Before designing a quantitative research study, you must decide whether it will be descriptive or experimental because this will dictate how you gather, analyze, and interpret the results. A descriptive study is governed by the following rules: subjects are generally measured once; the intention is to only establish associations between variables; and, the study may include a sample population of hundreds or thousands of subjects to ensure that a valid estimate of a generalized relationship between variables has been obtained. An experimental design includes subjects measured before and after a particular treatment, the sample population may be very small and purposefully chosen, and it is intended to establish causality between variables. Introduction The introduction to a quantitative study is usually written in the present tense and from the third person point of view. It covers the following information:

• Identifies the research problem -- as with any academic study, you must state clearly and concisely the research problem being investigated.
• Reviews the literature -- review scholarship on the topic, synthesizing key themes and, if necessary, noting studies that have used similar methods of inquiry and analysis. Note where key gaps exist and how your study helps to fill these gaps or clarifies existing knowledge.
• Describes the theoretical framework -- provide an outline of the theory or hypothesis underpinning your study. If necessary, define unfamiliar or complex terms, concepts, or ideas and provide the appropriate background information to place the research problem in proper context [e.g., historical, cultural, economic, etc.].

Methodology The methods section of a quantitative study should describe how each objective of your study will be achieved. Be sure to provide enough detail to enable the reader can make an informed assessment of the methods being used to obtain results associated with the research problem. The methods section should be presented in the past tense.

• Study population and sampling -- where did the data come from; how robust is it; note where gaps exist or what was excluded. Note the procedures used for their selection;
• Data collection – describe the tools and methods used to collect information and identify the variables being measured; describe the methods used to obtain the data; and, note if the data was pre-existing [i.e., government data] or you gathered it yourself. If you gathered it yourself, describe what type of instrument you used and why. Note that no data set is perfect--describe any limitations in methods of gathering data.
• Data analysis -- describe the procedures for processing and analyzing the data. If appropriate, describe the specific instruments of analysis used to study each research objective, including mathematical techniques and the type of computer software used to manipulate the data.

Results The finding of your study should be written objectively and in a succinct and precise format. In quantitative studies, it is common to use graphs, tables, charts, and other non-textual elements to help the reader understand the data. Make sure that non-textual elements do not stand in isolation from the text but are being used to supplement the overall description of the results and to help clarify key points being made. Further information about how to effectively present data using charts and graphs can be found here .

• Statistical analysis -- how did you analyze the data? What were the key findings from the data? The findings should be present in a logical, sequential order. Describe but do not interpret these trends or negative results; save that for the discussion section. The results should be presented in the past tense.

Discussion Discussions should be analytic, logical, and comprehensive. The discussion should meld together your findings in relation to those identified in the literature review, and placed within the context of the theoretical framework underpinning the study. The discussion should be presented in the present tense.

• Interpretation of results -- reiterate the research problem being investigated and compare and contrast the findings with the research questions underlying the study. Did they affirm predicted outcomes or did the data refute it?
• Description of trends, comparison of groups, or relationships among variables -- describe any trends that emerged from your analysis and explain all unanticipated and statistical insignificant findings.
• Discussion of implications – what is the meaning of your results? Highlight key findings based on the overall results and note findings that you believe are important. How have the results helped fill gaps in understanding the research problem?
• Limitations -- describe any limitations or unavoidable bias in your study and, if necessary, note why these limitations did not inhibit effective interpretation of the results.

Conclusion End your study by to summarizing the topic and provide a final comment and assessment of the study.

• Summary of findings – synthesize the answers to your research questions. Do not report any statistical data here; just provide a narrative summary of the key findings and describe what was learned that you did not know before conducting the study.
• Recommendations – if appropriate to the aim of the assignment, tie key findings with policy recommendations or actions to be taken in practice.
• Future research – note the need for future research linked to your study’s limitations or to any remaining gaps in the literature that were not addressed in your study.

Black, Thomas R. Doing Quantitative Research in the Social Sciences: An Integrated Approach to Research Design, Measurement and Statistics . London: Sage, 1999; Gay,L. R. and Peter Airasain. Educational Research: Competencies for Analysis and Applications . 7th edition. Upper Saddle River, NJ: Merril Prentice Hall, 2003; Hector, Anestine. An Overview of Quantitative Research in Composition and TESOL . Department of English, Indiana University of Pennsylvania; Hopkins, Will G. “Quantitative Research Design.” Sportscience 4, 1 (2000); "A Strategy for Writing Up Research Results. The Structure, Format, Content, and Style of a Journal-Style Scientific Paper." Department of Biology. Bates College; Nenty, H. Johnson. "Writing a Quantitative Research Thesis." International Journal of Educational Science 1 (2009): 19-32; Ouyang, Ronghua (John). Basic Inquiry of Quantitative Research . Kennesaw State University.

Strengths of Using Quantitative Methods

Quantitative researchers try to recognize and isolate specific variables contained within the study framework, seek correlation, relationships and causality, and attempt to control the environment in which the data is collected to avoid the risk of variables, other than the one being studied, accounting for the relationships identified.

Among the specific strengths of using quantitative methods to study social science research problems:

• Allows for a broader study, involving a greater number of subjects, and enhancing the generalization of the results;
• Allows for greater objectivity and accuracy of results. Generally, quantitative methods are designed to provide summaries of data that support generalizations about the phenomenon under study. In order to accomplish this, quantitative research usually involves few variables and many cases, and employs prescribed procedures to ensure validity and reliability;
• Applying well established standards means that the research can be replicated, and then analyzed and compared with similar studies;
• You can summarize vast sources of information and make comparisons across categories and over time; and,
• Personal bias can be avoided by keeping a 'distance' from participating subjects and using accepted computational techniques .

Babbie, Earl R. The Practice of Social Research . 12th ed. Belmont, CA: Wadsworth Cengage, 2010; Brians, Craig Leonard et al. Empirical Political Analysis: Quantitative and Qualitative Research Methods . 8th ed. Boston, MA: Longman, 2011; McNabb, David E. Research Methods in Public Administration and Nonprofit Management: Quantitative and Qualitative Approaches . 2nd ed. Armonk, NY: M.E. Sharpe, 2008; Singh, Kultar. Quantitative Social Research Methods . Los Angeles, CA: Sage, 2007.

Limitations of Using Quantitative Methods

Quantitative methods presume to have an objective approach to studying research problems, where data is controlled and measured, to address the accumulation of facts, and to determine the causes of behavior. As a consequence, the results of quantitative research may be statistically significant but are often humanly insignificant.

Some specific limitations associated with using quantitative methods to study research problems in the social sciences include:

• Quantitative data is more efficient and able to test hypotheses, but may miss contextual detail;
• Uses a static and rigid approach and so employs an inflexible process of discovery;
• The development of standard questions by researchers can lead to "structural bias" and false representation, where the data actually reflects the view of the researcher instead of the participating subject;
• Results provide less detail on behavior, attitudes, and motivation;
• Researcher may collect a much narrower and sometimes superficial dataset;
• Results are limited as they provide numerical descriptions rather than detailed narrative and generally provide less elaborate accounts of human perception;
• The research is often carried out in an unnatural, artificial environment so that a level of control can be applied to the exercise. This level of control might not normally be in place in the real world thus yielding "laboratory results" as opposed to "real world results"; and,
• Preset answers will not necessarily reflect how people really feel about a subject and, in some cases, might just be the closest match to the preconceived hypothesis.

Research Tip

Finding Examples of How to Apply Different Types of Research Methods

SAGE publications is a major publisher of studies about how to design and conduct research in the social and behavioral sciences. Their SAGE Research Methods Online and Cases database includes contents from books, articles, encyclopedias, handbooks, and videos covering social science research design and methods including the complete Little Green Book Series of Quantitative Applications in the Social Sciences and the Little Blue Book Series of Qualitative Research techniques. The database also includes case studies outlining the research methods used in real research projects. This is an excellent source for finding definitions of key terms and descriptions of research design and practice, techniques of data gathering, analysis, and reporting, and information about theories of research [e.g., grounded theory]. The database covers both qualitative and quantitative research methods as well as mixed methods approaches to conducting research.

SAGE Research Methods Online and Cases

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• What Is Quantitative Research? | Definition & Methods

What Is Quantitative Research? | Definition & Methods

Published on 4 April 2022 by Pritha Bhandari . Revised on 10 October 2022.

Quantitative research is the process of collecting and analysing numerical data. It can be used to find patterns and averages, make predictions, test causal relationships, and generalise results to wider populations.

Quantitative research is the opposite of qualitative research , which involves collecting and analysing non-numerical data (e.g. text, video, or audio).

Quantitative research is widely used in the natural and social sciences: biology, chemistry, psychology, economics, sociology, marketing, etc.

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Table of contents

Quantitative research methods, quantitative data analysis, advantages of quantitative research, disadvantages of quantitative research, frequently asked questions about quantitative research.

You can use quantitative research methods for descriptive, correlational or experimental research.

• In descriptive research , you simply seek an overall summary of your study variables.
• In correlational research , you investigate relationships between your study variables.
• In experimental research , you systematically examine whether there is a cause-and-effect relationship between variables.

Correlational and experimental research can both be used to formally test hypotheses , or predictions, using statistics. The results may be generalised to broader populations based on the sampling method used.

To collect quantitative data, you will often need to use operational definitions that translate abstract concepts (e.g., mood) into observable and quantifiable measures (e.g., self-ratings of feelings and energy levels).

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Once data is collected, you may need to process it before it can be analysed. For example, survey and test data may need to be transformed from words to numbers. Then, you can use statistical analysis to answer your research questions .

Descriptive statistics will give you a summary of your data and include measures of averages and variability. You can also use graphs, scatter plots and frequency tables to visualise your data and check for any trends or outliers.

Using inferential statistics , you can make predictions or generalisations based on your data. You can test your hypothesis or use your sample data to estimate the population parameter .

You can also assess the reliability and validity of your data collection methods to indicate how consistently and accurately your methods actually measured what you wanted them to.

Quantitative research is often used to standardise data collection and generalise findings . Strengths of this approach include:

• Replication

Repeating the study is possible because of standardised data collection protocols and tangible definitions of abstract concepts.

• Direct comparisons of results

The study can be reproduced in other cultural settings, times or with different groups of participants. Results can be compared statistically.

• Large samples

Data from large samples can be processed and analysed using reliable and consistent procedures through quantitative data analysis.

• Hypothesis testing

Using formalised and established hypothesis testing procedures means that you have to carefully consider and report your research variables, predictions, data collection and testing methods before coming to a conclusion.

Despite the benefits of quantitative research, it is sometimes inadequate in explaining complex research topics. Its limitations include:

• Superficiality

Using precise and restrictive operational definitions may inadequately represent complex concepts. For example, the concept of mood may be represented with just a number in quantitative research, but explained with elaboration in qualitative research.

• Narrow focus

Predetermined variables and measurement procedures can mean that you ignore other relevant observations.

• Structural bias

Despite standardised procedures, structural biases can still affect quantitative research. Missing data , imprecise measurements or inappropriate sampling methods are biases that can lead to the wrong conclusions.

• Lack of context

Quantitative research often uses unnatural settings like laboratories or fails to consider historical and cultural contexts that may affect data collection and results.

Quantitative research deals with numbers and statistics, while qualitative research deals with words and meanings.

Quantitative methods allow you to test a hypothesis by systematically collecting and analysing data, while qualitative methods allow you to explore ideas and experiences in depth.

In mixed methods research , you use both qualitative and quantitative data collection and analysis methods to answer your research question .

Data collection is the systematic process by which observations or measurements are gathered in research. It is used in many different contexts by academics, governments, businesses, and other organisations.

Operationalisation means turning abstract conceptual ideas into measurable observations.

For example, the concept of social anxiety isn’t directly observable, but it can be operationally defined in terms of self-rating scores, behavioural avoidance of crowded places, or physical anxiety symptoms in social situations.

Before collecting data , it’s important to consider how you will operationalise the variables that you want to measure.

Reliability and validity are both about how well a method measures something:

• Reliability refers to the  consistency of a measure (whether the results can be reproduced under the same conditions).
• Validity   refers to the  accuracy of a measure (whether the results really do represent what they are supposed to measure).

If you are doing experimental research , you also have to consider the internal and external validity of your experiment.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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Quantitative Research Methods

• Introduction
• Descriptive and Inferential Statistics
• Hypothesis Testing
• Regression and Correlation
• Time Series
• Meta-Analysis
• Mixed Methods
• Additional Resources
• Get Research Help

Welcome! This guide will help you to find resources about statistical methodologies often used across disciplines. It  provides basic descriptions of each statistical methodology and features web content, videos, and books. Please contact a librarian if you need help with quantitative methodology.  

Steps in the Quantitative Research Process

1. Become familiar with Research Data Management , which includes best practices for how to collect, store, analyze, preserve and share your data. You might want to consider creating a data management plan. 

2. Collect your data . You might have to enter data into a spreadsheet or find data online . Understanding the level of measurement that you need, the variables, and the sample size can be key.  

3. Clean your data by handling missing, incorrect, and duplicate values in appropriate ways.

4. Run descriptive statistics . No matter what statistical methodology you'll be employing in your analysis, it's important to run descriptive statistics.

5.  Select an inferential statistical method based on your research question and the characteristics of your data. Make sure to keep the descriptive statistics of your data in mind when selecting an inferential method.

7. Run analysis in statistical software such as SAS, SPSS, or R. 

8. Interpret your results.  

9. If you'd like to share your data with others, possibly by posting it on a repository, please contact a librarian to help you through that process.  

Glossary of Key Terms

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Research Methodology Example

Detailed Walkthrough + Free Methodology Chapter Template

If you’re working on a dissertation or thesis and are looking for an example of a research methodology chapter , you’ve come to the right place.

In this video, we walk you through a research methodology from a dissertation that earned full distinction , step by step. We start off by discussing the core components of a research methodology by unpacking our free methodology chapter template . We then progress to the sample research methodology to show how these concepts are applied in an actual dissertation, thesis or research project.

If you’re currently working on your research methodology chapter, you may also find the following resources useful:

• Research methodology 101 : an introductory video discussing what a methodology is and the role it plays within a dissertation
• Research design 101 : an overview of the most common research designs for both qualitative and quantitative studies
• Variables 101 : an introductory video covering the different types of variables that exist within research.
• Sampling 101 : an overview of the main sampling methods
• Methodology tips : a video discussion covering various tips to help you write a high-quality methodology chapter
• Private coaching : Get hands-on help with your research methodology

FAQ: Research Methodology Example

Research methodology example: frequently asked questions, is the sample research methodology real.

Yes. The chapter example is an extract from a Master’s-level dissertation for an MBA program. A few minor edits have been made to protect the privacy of the sponsoring organisation, but these have no material impact on the research methodology.

Can I replicate this methodology for my dissertation?

As we discuss in the video, every research methodology will be different, depending on the research aims, objectives and research questions. Therefore, you’ll need to tailor your literature review to suit your specific context.

You can learn more about the basics of writing a research methodology chapter here .

Where can I find more examples of research methodologies?

The best place to find more examples of methodology chapters would be within dissertation/thesis databases. These databases include dissertations, theses and research projects that have successfully passed the assessment criteria for the respective university, meaning that you have at least some sort of quality assurance.

The Open Access Thesis Database (OATD) is a good starting point.

How do I get the research methodology chapter template?

You can access our free methodology chapter template here .

Is the methodology template really free?

Yes. There is no cost for the template and you are free to use it as you wish.

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Your ultimate guide to quantitative research.

12 min read You may be already using quantitative research and want to check your understanding, or you may be starting from the beginning. Here’s an exploration of this research method and how you can best use it for maximum effect for your business.

You may be already using quantitative research and want to check your understanding, or you may be starting from the beginning. Here’s an exploration of this research method and how you can best use it for maximum effect for your business.

What is quantitative research?

Quantitative is the research method of collecting quantitative data – this is data that can be converted into numbers or numerical data, which can be easily quantified, compared, and analyzed.

Quantitative research deals with primary and secondary sources where data is represented in numerical form. This can include closed-question poll results, statistics, and census information or demographic data .

Quantitative data tends to be used when researchers are interested in understanding a particular moment in time and examining data sets over time to find trends and patterns.

To collect numerical data, surveys are often employed as one of the main research methods to source first-hand information in primary research . Quantitative research can also come from third-party research studies .

Quantitative research is widely used in the realms of social sciences, such as biology, chemistry, psychology, economics, sociology, and marketing .

Research teams collect data that is significant to proving or disproving a hypothesis research question – known as the research objective. When they collect quantitative data, researchers will aim to use a sample size that is representative of the total population of the target market they’re interested in.

Then the data collected will be manually or automatically stored and compared for insights.

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Quantitative vs qualitative research

While the quantitative research definition focuses on numerical data, qualitative research is defined as data that supplies non-numerical information.

Quantitative research focuses on the thoughts, feelings, and values of a participant , to understand why people act in the way they do . They result in data types like quotes, symbols, images, and written testimonials.

These data types tell researchers subjective information, which can help us assign people into categories, such as a participant’s religion, gender , social class, political alignment, likely favored products to buy, or their preferred training learning style.

For this reason, qualitative research is often used in social research, as this gives a window into the behavior and actions of people.

In general, if you’re interested in measuring something or testing a hypothesis, use quantitative methods. If you want to explore ideas, thoughts, and meanings, use qualitative methods.

However, quantitative and qualitative research methods are both recommended when you’re looking to understand a point in time, while also finding out the reason behind the facts.

Quantitative research data collection methods

Quantitative research methods can use structured research instruments like:

• Surveys : A survey is a simple-to-create and easy-to-distribute research method , which helps gather information from large groups of participants quickly. Traditionally, paper-based surveys can now be made online, so costs can stay quite low.

Quantitative questions tend to be closed questions that ask for a numerical result, based on a range of options, or a yes/no answer that can be tallied quickly.

• Face-to-face or phone interviews: Interviews are a great way to connect with participants , though they require time from the research team to set up and conduct.

Researchers may also have issues connecting with participants in different geographical regions . The researcher uses a set of predefined close-ended questions, which ask for yes/no or numerical values.

• Polls: Polls can be a shorter version of surveys , used to get a ‘flavor’ of what the current situation is with participants. Online polls can be shared easily, though polls are best used with simple questions that request a range or a yes/no answer.

Quantitative data is the opposite of qualitative research, another dominant framework for research in the social sciences, explored further below.

Quantitative data types

Quantitative research methods often deliver the following data types:

• Test Scores
• Percent of training course completed
• Performance score out of 100
• Number of support calls active
• Customer Net Promoter Score (NPS)

When gathering numerical data, the emphasis is on how specific the data is, and whether they can provide an indication of what ‘is’ at the time of collection. Pre-existing statistical data can tell us what ‘was’ for the date and time range that it represented

Quantitative research design methods (with examples)

Quantitative research has a number of quantitative research designs you can choose from:

Descriptive

This design type describes the state of a data type is telling researchers, in its native environment. There won’t normally be a clearly defined research question to start with. Instead, data analysis will suggest a conclusion , which can become the hypothesis to investigate further.

Examples of descriptive quantitative design include:

• A description of child’s Christmas gifts they received that year
• A description of what businesses sell the most of during Black Friday
• A description of a product issue being experienced by a customer

Correlational

This design type looks at two or more data types, the relationship between them, and the extent that they differ or align. This does not look at the causal links deeper – instead statistical analysis looks at the variables in a natural environment.

Examples of correlational quantitative design include:

• The relationship between a child’s Christmas gifts and their perceived happiness level
• The relationship between a business’ sales during Black Friday and the total revenue generated over the year
• The relationship between a customer’s product issue and the reputation of the product

Causal-Comparative/Quasi-Experimental

This design type looks at two or more data types and tries to explain any relationship and differences between them, using a cause-effect analysis. The research is carried out in a near-natural environment, where information is gathered from two groups – a naturally occurring group that matches the original natural environment, and one that is not naturally present.

This allows for causal links to be made, though they might not be correct, as other variables may have an impact on results.

Examples of causal-comparative/quasi-experimental quantitative design include:

• The effect of children’s Christmas gifts on happiness
• The effect of Black Friday sales figures on the productivity of company yearly sales
• The effect of product issues on the public perception of a product

Experimental Research

This design type looks to make a controlled environment in which two or more variables are observed to understand the exact cause and effect they have. This becomes a quantitative research study, where data types are manipulated to assess the effect they have. The participants are not naturally occurring groups, as the setting is no longer natural. A quantitative research study can help pinpoint the exact conditions in which variables impact one another.

Examples of experimental quantitative design include:

• The effect of children’s Christmas gifts on a child’s dopamine (happiness) levels
• The effect of Black Friday sales on the success of the company
• The effect of product issues on the perceived reliability of the product

Quantitative research methods need to be carefully considered, as your data collection of a data type can be used to different effects. For example, statistics can be descriptive or correlational (or inferential). Descriptive statistics help us to summarize our data, while inferential statistics help infer conclusions about significant differences.

Advantages of quantitative research

• Easy to do : Doing quantitative research is more straightforward, as the results come in numerical format, which can be more easily interpreted.
• Less interpretation : Due to the factual nature of the results, you will be able to accept or reject your hypothesis based on the numerical data collected.
• Less bias : There are higher levels of control that can be applied to the research, so bias can be reduced , making your data more reliable and precise.

Disadvantages of quantitative research

• Can’t understand reasons: Quantitative research doesn’t always tell you the full story, meaning you won’t understand the context – or the why, of the data you see, why do you see the results you have uncovered?
• Useful for simpler situations: Quantitative research on its own is not great when dealing with complex issues. In these cases, quantitative research may not be enough.

How to use quantitative research to your business’s advantage

Quantitative research methods may help in areas such as:

• Identifying which advert or landing page performs better
• Identifying how satisfied your customers are
• How many customers are likely to recommend you
• Tracking how your brand ranks in awareness and customer purchase intent
• Learn what consumers are likely to buy from your brand.

6 steps to conducting good quantitative research

Businesses can benefit from quantitative research by using it to evaluate the impact of data types. There are several steps to this:

• Define your problem or interest area : What do you observe is happening and is it frequent? Identify the data type/s you’re observing.
• Create a hypothesis : Ask yourself what could be the causes for the situation with those data types.
• Plan your quantitative research : Use structured research instruments like surveys or polls to ask questions that test your hypothesis.
• Data Collection : Collect quantitative data and understand what your data types are telling you. Using data collected on different types over long time periods can give you information on patterns.
• Data analysis : Does your information support your hypothesis? (You may need to redo the research with other variables to see if the results improve)
• Effectively present data : Communicate the results in a clear and concise way to help other people understand the findings.

How Qualtrics products can enhance & simplify the quantitative research process

The Qualtrics XM system gives you an all-in-one, integrated solution to help you all the way through conducting quantitative research. From survey creation and data collection to statistical analysis and data reporting, it can help all your internal teams gain insights from your numerical data.

Quantitative methods are catered to your business through templates or advanced survey designs. While you can manually collect data and conduct data analysis in a spreadsheet program, this solution helps you automate the process of quantitative research, saving you time and administration work.

Using computational techniques helps you to avoid human errors, and participant results come in are already incorporated into the analysis in real-time.

Our key tools, Stats IQ™ and Driver IQ™ make analyzing numerical data easy and simple. Choose to highlight key findings based on variables or highlight statistically insignificant findings. The choice is yours.

Some examples of your workspace in action, using drag and drop to create fast data visualizations quickly:

Related resources

Market intelligence 10 min read, marketing insights 11 min read, ethnographic research 11 min read, qualitative vs quantitative research 13 min read, qualitative research questions 11 min read, qualitative research design 12 min read, primary vs secondary research 14 min read, request demo.

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What is Quantitative Research? Definition, Examples, Key Advantages, Methods and Best Practices

By Nick Jain

Published on: May 17, 2023

Table of Contents

What is Quantitative Research?

Quantitative research examples, quantitative research: key advantages, quantitative research methodology, 7 best practices to conduct quantitative research.

Quantitative research stands as a powerful research methodology dedicated to the systematic collection and analysis of measurable data. Through rigorous statistical and mathematical techniques, this method extracts insights from structured surveys, controlled experiments, or other defined data-gathering methods.

The primary objective of quantitative research is to measure and quantify variables, relationships, and patterns within the dataset. By testing hypotheses, making predictions, and drawing generalizable conclusions, it plays a crucial role in fields such as psychology, sociology, economics, and education. This approach often involves significant sample sizes, ensuring robust results.

Explore the depth of quantitative research with this comprehensive guide, offering practical examples and applications to demonstrate its real-world impact. Stay updated with the latest trends and developments in quantitative research as we continually refine our insights to provide you with the most relevant and cutting-edge information.

Quantitative Research: Key Characteristics

Below are the key characteristics of quantitative research:

• Objectivity: Quantitative research is grounded in the principles of objectivity and empiricism, which means that the research is focused on observable and measurable phenomena, rather than personal opinions or experiences.
• Structured approach: Quantitative research follows a structured and systematic approach to data collection and analysis, using clearly defined variables, hypotheses, and research questions.
• Numeric data: Quantitative research uses numerical data to describe and analyze the phenomena under study, such as statistical analysis, surveys, and experiments.
• Large sample size: Quantitative research often involves large sample sizes to ensure statistical significance and to generalize findings to a larger population.
• Standardized data collection: Quantitative research typically involves standardized data collection methods, such as surveys or experiments, to minimize potential sources of bias and increase reliability.
• Deductive reasoning: Quantitative research uses deductive reasoning, where the researcher tests a specific hypothesis based on prior knowledge and theory.
• Replication: Quantitative research emphasizes the importance of replication, where other researchers can reproduce the study’s methods and obtain similar results.
• Statistical analysis: Quantitative research involves statistical analysis to analyze the data and test the research hypotheses, often using software programs to assist with data analysis.
• Precision: Quantitative research aims to be precise in its measurement and analysis of data. It seeks to quantify and measure the specific aspects of a phenomenon being studied.
• Generalizability: Quantitative research aims to generalize findings from a sample to a larger population. It seeks to draw conclusions that apply to a broader group beyond the specific sample being studied.

Below are 3 examples of quantitative research:

Example 1: Boosting Employee Performance with Innovative Training Programs

In this quantitative study, we delve into the transformative impact of a cutting-edge training program on employee productivity within corporate environments. Employing a quasi-experimental framework, we meticulously analyze the outcomes of a cohort undergoing innovative training against a control group. Through advanced statistical methodologies, we unveil actionable insights into performance enhancements, arming organizations with data-driven strategies for workforce development and competitive advantage.

Example 2: Unveiling the Power of Physical Exercise on Mental Well-being

Unlocking the correlation between physical exercise and mental health, this quantitative inquiry stands at the forefront of holistic wellness research. Through meticulous data collection and rigorous statistical analyses, we dissect the nuanced relationship between exercise regimens and mental well-being indicators. Our findings not only underscore the profound impact of exercise on psychological resilience but also provide actionable insights for healthcare professionals and individuals striving for optimal mental health.

Example 3: Revolutionizing Education with Innovative Teaching Methodologies

In this groundbreaking study, we embark on a quantitative exploration of the transformative potential of innovative teaching methods on student learning outcomes. Utilizing a quasi-experimental design, we meticulously evaluate the efficacy of novel pedagogical approaches against conventional teaching methodologies. Through rigorous statistical analyses of pre-test and post-test data, we unearth compelling evidence of enhanced academic performance, paving the way for educational institutions to embrace innovation and elevate learning experiences.

Learn more: What is Quantitative Market Research?

The advantages of quantitative research make it a valuable research method in a variety of fields, particularly in fields that require precise measurement and testing of hypotheses.

• Precision: Quantitative research aims to be precise in its measurement and analysis of data. This can increase the accuracy of the results and enable researchers to make more precise predictions.
• Test hypotheses: Quantitative research is well-suited for testing specific hypotheses or research questions, allowing researchers to draw clear conclusions and make predictions based on the data.
• Quantify relationships: Quantitative research enables researchers to quantify and measure relationships between variables, allowing for more precise and quantitative comparisons.
• Efficiency: Quantitative research often involves the use of standardized procedures and data collection methods, which can make the research process more efficient and reduce the amount of time and resources required.
• Easy to compare: Quantitative research often involves the use of standardized measures and scales, which makes it easier to compare results across different studies or populations.
• Ability to detect small effects: Quantitative research is often able to detect small effects that may not be observable through qualitative research methods, due to the use of statistical analysis and large sample sizes.

Quantitative research is a type of research that focuses on collecting and analyzing numerical data to answer research questions. There are two main methods used to conduct quantitative research:

1. Primary Method

There are several methods of primary quantitative research, each with its own strengths and limitations.

Surveys: Surveys are a common method of quantitative research and involve collecting data from a sample of individuals using standardized questionnaires or interviews. Surveys can be conducted in various ways, such as online, by mail, by phone, or in person. Surveys can be used to study attitudes, behaviors, opinions, and demographics.

One of the main advantages of surveys is that they can be conducted on a large scale, making it possible to obtain representative data from a population. However, surveys can suffer from issues such as response bias, where participants may not provide accurate or truthful answers, and nonresponse bias, where certain groups may be less likely to participate in the survey.

Experiments: Experiments involve manipulating one or more variables to determine their effects on an outcome of interest. Experiments can be carried out in controlled laboratory settings or in real-world field environments. Experiments can be used to test causal relationships between variables and to establish cause-and-effect relationships.

One of the main advantages of experiments is that they provide a high level of control over the variables being studied, which can increase the internal validity of the study. However, experiments can suffer from issues such as artificiality, where the experimental setting may not accurately reflect real-world situations, and demand characteristics, where participants may change their behavior due to the experimental setting.

Observational studies: Observational studies involve observing and recording data without manipulating any variables. Observational studies can be conducted in various settings, such as naturalistic environments or controlled laboratory settings. Observational studies can be used to study behaviors, interactions, and phenomena that cannot be manipulated experimentally.

One of the main advantages of observational studies is that they can provide rich and detailed data about real-world phenomena. However, observational studies can suffer from issues such as observer bias, where the observer may interpret the data in a subjective manner, and reactivity, where the presence of the observer may change the behavior being observed.

Content analysis: Content analysis involves analyzing media or communication content, such as text, images, or videos, to identify patterns or trends. Content analysis can be used to study media representations of social issues or to identify patterns in social media data.

One of the main advantages of content analysis is that it can provide insights into the cultural and social values reflected in media content. However, content analysis can suffer from issues such as the subjectivity of the coding process and the potential for errors or bias in the data collection process.

Psychometrics: Psychometrics involves the development and validation of standardized tests or measures, such as personality tests or intelligence tests. Psychometrics can be used to study individual differences in psychological traits and to assess the validity and reliability of psychological measures.

One of the main advantages of psychometrics is that it can provide a standardized and objective way to measure psychological constructs. However, psychometrics can suffer from issues such as the cultural specificity of the measures and the potential for response bias in self-report measures.

2. Secondary Method

Secondary quantitative research methods involve analyzing existing data that was collected for other purposes. This can include data from government records, public opinion polls, or market research studies. Secondary research is often quicker and less expensive than primary research, but it may not provide data that is as specific to the research question.

One of the main advantages of secondary data analysis is that it can be a cost-effective way to obtain large amounts of data. However, secondary data analysis can suffer from issues such as the quality and relevance of the data, and the potential for missing or incomplete data.

Learn more: What is Quantitative Observation?

Here are the key best practices that should be followed when conducting quantitative research:

1. Clearly define the research question: The research question should be specific, measurable, and focused on a clear problem or issue.

2. Use a well-designed research design: The research design should be appropriate for the research question, and should include a clear sampling strategy, data collection methods, and statistical analysis plan.

3. Use validated and reliable instruments: The instruments used to collect data should be validated and reliable to ensure that the data collected is accurate and consistent.

4. Ensure informed consent: Participants should be fully informed about the purpose of the research, their rights, and how their data will be used. Informed consent should be obtained before data collection begins.

5. Minimize bias: Researchers should take steps to minimize bias in all stages of the research process, including study design, data collection, and data analysis.

6. Ensure data security and confidentiality: Data should be kept secure and confidential to protect the privacy of participants and prevent unauthorized access.

7. Use appropriate statistical analysis: Statistical analysis should be appropriate for the research question and the data collected. Accurate and clear reporting of results is imperative in quantitative research.

Learn more: What is Qualitative Research?

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Quantitative and Qualitative Research

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Quantitative methodology is the dominant research framework in the social sciences. It refers to a set of strategies, techniques and assumptions used to study psychological, social and economic processes through the exploration of numeric patterns . Quantitative research gathers a range of numeric data. Some of the numeric data is intrinsically quantitative (e.g. personal income), while in other cases the numeric structure is  imposed (e.g. ‘On a scale from 1 to 10, how depressed did you feel last week?’). The collection of quantitative information allows researchers to conduct simple to extremely sophisticated statistical analyses that aggregate the data (e.g. averages, percentages), show relationships among the data (e.g. ‘Students with lower grade point averages tend to score lower on a depression scale’) or compare across aggregated data (e.g. the USA has a higher gross domestic product than Spain). Quantitative research includes methodologies such as questionnaires, structured observations or experiments and stands in contrast to qualitative research. Qualitative research involves the collection and analysis of narratives and/or open-ended observations through methodologies such as interviews, focus groups or ethnographies.

Coghlan, D., Brydon-Miller, M. (2014).  The SAGE encyclopedia of action research  (Vols. 1-2). London, : SAGE Publications Ltd doi: 10.4135/9781446294406

What is the purpose of quantitative research?

The purpose of quantitative research is to generate knowledge and create understanding about the social world. Quantitative research is used by social scientists, including communication researchers, to observe phenomena or occurrences affecting individuals. Social scientists are concerned with the study of people. Quantitative research is a way to learn about a particular group of people, known as a sample population. Using scientific inquiry, quantitative research relies on data that are observed or measured to examine questions about the sample population.

Allen, M. (2017).  The SAGE encyclopedia of communication research methods  (Vols. 1-4). Thousand Oaks, CA: SAGE Publications, Inc doi: 10.4135/9781483381411

How do I know if the study is a quantitative design?  What type of quantitative study is it?

Quantitative Research Designs: Descriptive non-experimental, Quasi-experimental or Experimental?

Studies do not always explicitly state what kind of research design is being used.  You will need to know how to decipher which design type is used.  The following video will help you determine the quantitative design type.

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A Comprehensive Guide to Quantitative Research: Types, Characteristics, Methods & Examples

Step into the fascinating world of quantitative research, where numbers reveal extraordinary insights!

By gathering and studying data in a systematic way, quantitative research empowers us to understand our ever-changing world better. It helps understand a problem or an already-formed hypothesis by generating numerical data. The results don’t end here, as you can process these numbers to get actionable insights that aid decision-making.

You can use quantitative research to quantify opinions, behaviors, attitudes, and other definitive variables related to the market, customers, competitors, etc. The research is conducted on a larger sample population to draw predictive, average, and pattern-based insights.

Here, we delve into the intricacies of this research methodology, exploring various quantitative methods, their advantages, and real-life examples that showcase their impact and relevance.

Ready to embark on a journey of discovery and knowledge? Let’s go!

What Is Quantitative Research?

Quantitative research is a method that uses numbers and statistics to test theories about customer attitudes and behaviors. It helps researchers gather and analyze data systematically to gain valuable insights and draw evidence-based conclusions about customer preferences and trends.

Researchers use online surveys , questionnaires , polls , and quizzes to question a large number of people to obtain measurable and bias-free data.

In technical terms, quantitative research is mainly concerned with discovering facts about social phenomena while assuming a fixed and measurable reality.

Offering numbers and stats-based insights, this research methodology is a crucial part of primary research and helps understand how well an organizational decision is going to work out.

Let’s consider an example.

Suppose your qualitative analysis shows that your customers are looking for social media-based customer support . In that case, quantitative analysis will help you see how many of your customers are looking for this support.

If 10% of your customers are looking for such a service, you might or might not consider offering this feature. But, if 40% of your regular customers are seeking support via social media, then it is something you just cannot overlook.

Characteristics of Quantitative Research

Quantitative research clarifies the fuzziness of research data from qualitative research analysis. With numerical insights, you can formulate a better and more profitable business decision.

Hence, quantitative research is more readily contestable, sharpens intelligent discussion, helps you see the rival hypotheses, and dynamically contributes to the research process.

Let us have a quick look at some of its characteristics.

• Measurable Variables

The data collection methods in quantitative research are structured and contain items requiring measurable variables, such as age, number of family members, salary range, highest education, etc.

These structured data collection methods comprise polls, surveys, questionnaires, etc., and may have questions like the ones shown in the following image:

As you can see, all the variables are measurable. This ensures that the research is in-depth and provides less erroneous data for reliable, actionable insights.

• Sample Size

No matter what data analysis methods for quantitative research are being used, the sample size is kept such that it represents the target market.

As the main aim of the research methodology is to get numerical insights, the sample size should be fairly large. Depending on the survey objective and scope, it might span hundreds of thousands of people.

• Normal Population Distribution

To maintain the reliability of a quantitative research methodology, we assume that the population distribution curve is normal.

This type of population distribution curve is preferred over a non-normal distribution as the sample size is large, and the characteristics of the sample vary with its size.

This requires adhering to the random sampling principle to avoid the researcher’s bias in result interpretation. Any bias can ruin the fairness of the entire process and defeats the purpose of research.

• Well-Structured Data Representation

Data analysis in quantitative research produces highly structured results and can form well-defined graphical representations. Some common examples include tables, figures, graphs, etc., that combine large blocks of data.

This way, you can discover hidden data trends, relationships, and differences among various measurable variables. This can help researchers understand the survey data and formulate actionable insights for decision-making.

• Predictive Outcomes

Quantitative analysis of data can also be used for estimations and prediction outcomes. You can construct if-then scenarios and analyze the data for the identification of any upcoming trends or events.

However, this requires advanced analytics and involves complex mathematical computations. So, it is mostly done via quantitative research tools that come with advanced analytics capabilities.

8 Best Practices to Conduct Quantitative Research

Here are some best practices to keep in mind while conducting quantitative research:

1. Define Research Objectives

There can be many ways to collect data via quantitative research methods that are chosen as per the research objective and scope. These methods allow you to build your own observations regarding any hypotheses – unknown, entirely new, or unexplained. 

You can hypothesize a proof and build a prediction of outcomes supporting the same. You can also create a detailed stepwise plan for data collection, analysis, and testing. 

Below, we explore quantitative research methods and discuss some examples to enhance your understanding of them.

2. Keep Your Questions Simple

The surveys are meant to reach people en-masse, and that includes a wide demographic range with recipients from all walks of life. Asking simple questions will ensure that they grasp what’s being asked easily.

Read More: Proven Tips to Avoid Leading and Loaded Questions in Your Survey

3. Develop a Solid Research Design

Choose an appropriate research design that aligns with your objectives, whether it’s experimental, quasi-experimental, or correlational. You also need to pay attention to the sample size and sampling technique such that it represents the target population accurately.

4. Use Reliable & Valid Instruments

It’s crucial to select or develop measurement instruments such as questionnaires, scales, or tests that have been validated and are reliable. Before proceeding with the main study, pilot-test these instruments on a small sample to assess their effectiveness and make any necessary improvements.

5. Ensure Data Quality

Implement data collection protocols to minimize errors and bias during data gathering. Double-check data entries and cleaning procedures to eliminate any inconsistencies or missing values that may affect the accuracy of your results. For instance, you might regularly cross-verify data entries to identify and correct any discrepancies.

6. Employ Appropriate Data Analysis Techniques

Select statistical methods that match the nature of your data and research questions. Whether it’s regression analysis, t-tests, ANOVA, or other techniques, using the right approach is important for drawing meaningful conclusions. Utilize software tools like SPSS or R for data analysis to ensure the accuracy and reproducibility of your findings.

7. Interpret Results Objectively

Present your findings in a clear and unbiased manner. Avoid making unwarranted causal claims, especially in correlational studies. Instead, focus on describing the relationships and patterns observed in your data.

8. Address Ethical Considerations

Prioritize ethical considerations throughout your research process. Obtain informed consent from participants, ensuring their voluntary participation and confidentiality of data. Comply with ethical guidelines and gain approval from a governing body if necessary.

Read More: How to Find Survey Participants & Respondents

Types of Quantitative Research Methods

Quantitative research is usually conducted using two methods. They are-

• Primary quantitative research methods
• Secondary quantitative research methods

1. Primary Methods

Primary quantitative research is the most popular way of conducting market research. The differentiating factor of this method is that the researcher relies on collecting data firsthand instead of relying on data collected from previous research.

There are multiple types of primary quantitative research. They can be distinguished based on three distinctive aspects, which are:

A. Techniques & Types of Studies:

• Survey Research

Surveys are the easiest, most common, and one of the most sought-after quantitative research techniques. The main aim of a survey is to widely gather and describe the characteristics of a target population or customers. Surveys are the foremost quantitative method preferred by both small and large organizations.

They help them understand their customers, products, and other brand offerings in a proper manner.

Surveys can be conducted using various methods, such as online polls, web-based surveys, paper questionnaires, phone calls, or face-to-face interviews. Survey research allows organizations to understand customer opinions, preferences, and behavior, making it crucial for market research and decision-making.

You can watch this quick video to learn more about creating surveys.

Surveys are of two types:

• Cross-Sectional Surveys Cross-sectional surveys are used to collect data from a sample of the target population at a specific point in time. Researchers evaluate various variables simultaneously to understand the relationships and patterns within the data.
• Cross-sectional surveys are popular in retail, small and medium-sized enterprises (SMEs), and healthcare industries, where they assess customer satisfaction, market trends, and product feedback.
• Longitudinal Surveys Longitudinal surveys are conducted over an extended period, observing changes in respondent behavior and thought processes.
• Researchers gather data from the same sample multiple times, enabling them to study trends and developments over time. These surveys are valuable in fields such as medicine, applied sciences, and market trend analysis.

Surveys can be distributed via various channels. Some of the most popular ones are listed below:

• Email: Sending surveys via email is a popular and effective method. People recognize your brand, leading to a higher response rate. With ProProfs Survey Maker’s in-mail survey-filling feature, you can easily send out and collect survey responses.
• Embed on a website: Boost your response rate by embedding the survey on your website. When visitors are already engaged with your brand, they are more likely to take the survey.
• Social media: Take advantage of social media platforms to distribute your survey. People familiar with your brand are likely to respond, increasing your response numbers.
• QR codes: QR codes store your survey’s URL, and you can print or publish these codes in magazines, signs, business cards, or any object to make it easy for people to access your survey.
• SMS survey: Collect a high number of responses quickly with SMS surveys. It’s a time-effective way to reach your target audience.

Read More: 24 Different Types of Survey Methods With Examples

2. Correlational Research:

Correlational research aims to establish relationships between two or more variables.

Researchers use statistical analysis to identify patterns and trends in the data, but it does not determine causality between the variables. This method helps understand how changes in one variable may impact another.

Examples of correlational research questions include studying the relationship between stress and depression, fame and money, or classroom activities and student performance.

3. Causal-Comparative Research:

Causal-comparative research, also known as quasi-experimental research, seeks to determine cause-and-effect relationships between variables.

Researchers analyze how an independent variable influences a dependent variable, but they do not manipulate the independent variable. Instead, they observe and compare different groups to draw conclusions.

Causal-comparative research is useful in situations where it’s not ethical or feasible to conduct true experiments.

Examples of questions for this type of research include analyzing the effect of training programs on employee performance, studying the influence of customer support on client retention, investigating the impact of supply chain efficiency on cost reduction, etc.

4. Experimental Research:

Experimental research is based on testing theories to validate or disprove them. Researchers conduct experiments and manipulate variables to observe their impact on the outcomes.

This type of research is prevalent in natural and social sciences, and it is a powerful method to establish cause-and-effect relationships. By randomly assigning participants to experimental and control groups, researchers can draw more confident conclusions.

Examples of experimental research include studying the effectiveness of a new drug, the impact of teaching methods on student performance, or the outcomes of a marketing campaign.

B. Data collection methodologies

After defining research objectives, the next significant step in primary quantitative research is data collection. This involves using two main methods: sampling and conducting surveys or polls.

Sampling methods:

In quantitative research, there are two primary sampling methods: Probability and Non-probability sampling.

Probability Sampling

In probability sampling, researchers use the concept of probability to create samples from a population. This method ensures that every individual in the target audience has an equal chance of being selected for the sample.

There are four main types of probability sampling:

• Simple random sampling: Here, the elements or participants of a sample are selected randomly, and this technique is used in studies that are conducted over considerably large audiences. It works well for large target populations.
• Stratified random sampling: In this method, the entire population is divided into strata or groups, and the sample members get chosen randomly from these strata only. It is always ensured that different segregated strata do not overlap with each other.
• Cluster sampling: Here, researchers divide the population into clusters, often based on geography or demographics. Then, random clusters are selected for the sample.
• Systematic sampling: In this method, only the starting point of the sample is randomly chosen. All the other participants are chosen using a fixed interval. Researchers calculate this interval by dividing the size of the study population by the target sample size.

Non-probability Sampling

Non-probability sampling is a method where the researcher’s knowledge and experience guide the selection of samples. This approach doesn’t give all members of the target population an equal chance of being included in the sample.

There are five non-probability sampling models:

• Convenience sampling: The elements or participants are chosen on the basis of their nearness to the researcher. The people in close proximity can be studied and analyzed easily and quickly, as there is no other selection criterion involved. Researchers simply choose samples based on what is most convenient for them.
• Consecutive sampling: Similar to convenience sampling, researchers select samples one after another over a significant period. They can opt for a single participant or a group of samples to conduct quantitative research in a consecutive manner for a significant period of time. Once this is over, they can conduct the research from the start.
• Quota sampling: With quota sampling, researchers use their understanding of target traits and personalities to form groups (strata). They then choose samples from each stratum based on their own judgment.
• Snowball sampling: This method is used where the target audiences are difficult to contact and interviewed for data collection. Researchers start with a few participants and then ask them to refer others, creating a snowball effect.
• Judgmental sampling: In judgmental sampling, researchers rely solely on their experience and research skills to handpick samples that they believe will be most relevant to the study.

Read More: Data Collection Methods: Definition, Types & Examples

C. Data analysis techniques

To analyze the quantitative data accurately, you’ll need to use specific statistical methods such as:

• SWOT Analysis: This stands for Strengths, Weaknesses, Opportunities, and Threats analysis. Organizations use SWOT analysis to evaluate their performance internally and externally. It helps develop effective improvement strategies.
• Conjoint Analysis: This market research method uncovers how individuals make complex purchasing decisions. It involves considering trade-offs in their daily activities when choosing from a list of product/service options.
• Cross-tabulation: A preliminary statistical market analysis method that reveals relationships, patterns, and trends within various research study parameters.
• TURF Analysis: Short for Totally Unduplicated Reach and Frequency Analysis, this method helps analyze the reach and frequency of favorable communication sources. It provides insights into the potential of a target market.
• By using these statistical techniques and inferential statistics methods like confidence intervals and margin of error, you can draw meaningful insights from your primary quantitative research that you can use in making informed decisions.

II. Secondary Quantitative Research Methods

• Secondary quantitative research, also known as desk research, is a valuable method that uses existing data, called secondary data.
• Instead of collecting new data, researchers analyze and combine already available information to enhance their research. This approach involves gathering quantitative data from various sources such as the internet, government databases, libraries, and research reports.
• Secondary quantitative research plays a crucial role in validating data collected through primary quantitative research. It helps reinforce or challenge existing findings.

Here are five commonly used secondary quantitative research methods:

A. Data Available on the Internet:

The Internet has become a vast repository of data, making it easier for researchers to access a wealth of information. Online databases, websites, and research repositories provide valuable quantitative data for researchers to analyze and validate their primary research findings.

B. Government and Non-Government Sources:

Government agencies and non-government organizations often conduct extensive research and publish reports. These reports cover a wide range of topics, providing researchers with reliable and comprehensive data for quantitative analysis.

C. Public Libraries:

While less commonly used in the digital age, public libraries still hold valuable research reports, historical data, and publications that can contribute to quantitative research.

D. Educational Institutions:

Educational institutions frequently conduct research on various subjects. Their research reports and publications can serve as valuable sources of information for researchers, validating and supporting primary quantitative research outcomes.

E. Commercial Information Sources:

Commercial sources such as local newspapers, journals, magazines, and media outlets often publish relevant data on economic trends, market research, and demographic analyses. Researchers can access this data to supplement their own findings and draw better conclusions.

Advantages of Quantitative Research Methods

Quantitative research data is often standardized and can be easily used to generalize findings for making crucial business decisions and uncover insights to supplement the qualitative research findings.

Here are some core benefits this research methodology offers.

Direct Result Comparison

As the studies can be replicated for different cultural settings and different times, even with different groups of participants, they tend to be extremely useful. Researchers can compare the results of different studies in a statistical manner and arrive at comprehensive conclusions for a broader understanding.

Replication

Researchers can repeat the study by using standardized data collection protocols over well-structured data sets. They can also apply tangible definitions of abstract concepts to arrive at different conclusions for similar research objectives with minor variations.

Large Samples

As the research data comes from large samples, the researchers can process and analyze the data via highly reliable and consistent analysis procedures. They can arrive at well-defined conclusions that can be used to make the primary research more thorough and reliable.

Hypothesis Testing

This research methodology follows standardized and established hypothesis testing procedures. So, you have to be careful while reporting and analyzing your research data , and the overall quality of results gets improved.

Proven Examples of Quantitative Research Methods

Below, we discuss two excellent examples of quantitative research methods that were used by highly distinguished business and consulting organizations. Both examples show how different types of analysis can be performed with qualitative approaches and how the analysis is done once the data is collected.

1. STEP Project Global Consortium / KPMG 2019 Global Family Business survey

This research utilized quantitative methods to identify ways that kept the family businesses sustainably profitable with time.

The study also identified the ways in which the family business behavior changed with demographic changes and had “why” and “how” questions. Their qualitative research methods allowed the KPMG team to dig deeper into the mindsets and perspectives of the business owners and uncover unexpected research avenues as well.

It was a joint effort in which STEP Project Global Consortium collected 26 cases, and KPMG collected 11 cases.

The research reached the stage of data analysis in 2020, and the analysis process spanned over 4 stages.

The results, which were also the reasons why family businesses tend to lose their strength with time, were found to be:

• Family governance
• Family business legacy

2. EY Seren Teams Research 2020

This is yet another commendable example of qualitative research where the EY Seren Team digs into the unexplored depths of human behavior and how it affected their brand or service expectations.

The research was done across 200+ sources and involved in-depth virtual interviews with people in their homes, exploring their current needs and wishes. It also involved diary studies across the entire UK customer base to analyze human behavior changes and patterns.

The study also included interviews with professionals and design leaders from a wide range of industries to explore how COVID-19 transformed their industries. Finally, quantitative surveys were conducted to gain insights into the EY community after every 15 days.

The insights and results were:

• A culture of fear, daily resilience, and hopes for a better world and a better life – these were the macro trends.
• People felt massive digitization to be a resourceful yet demanding aspect as they have to adapt every day.
• Some people wished to have a new world with lots of possibilities, and some were looking for a new purpose.

Enhance Your Quantitative Research With Cutting-Edge Software

While no single research methodology can produce 100% reliable results, you can always opt for a hybrid research method by opting for the methods that are most relevant to your objective.

This understanding comes gradually as you learn how to implement the correct combination of qualitative and quantitative research methods for your research projects. For the best results, we recommend investing in smart, efficient, and scalable research tools that come with delightful reporting and advanced analytics to make every research initiative a success.

These software tools, such as ProProfs Survey Maker, come with pre-built survey templates and question libraries and allow you to create a high-converting survey in just a few minutes.

So, choose the best research partner, create the right research plan, and gather insights that drive sustainable growth for your business.

About the author

Jared cornell.

Jared is a customer support expert. He has been published in CrazyEgg , Foundr , and CXL . As a customer support executive at ProProfs, he has been instrumental in developing a complete customer support system that more than doubled customer satisfaction. You can connect and engage with Jared on Twitter , Facebook , and LinkedIn .

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Library Guides

Dissertations 4: methodology: methods.

• Introduction & Philosophy
• Methodology

Primary & Secondary Sources, Primary & Secondary Data

When describing your research methods, you can start by stating what kind of secondary and, if applicable, primary sources you used in your research. Explain why you chose such sources, how well they served your research, and identify possible issues encountered using these sources.  

Definitions  

There is some confusion on the use of the terms primary and secondary sources, and primary and secondary data. The confusion is also due to disciplinary differences (Lombard 2010). Whilst you are advised to consult the research methods literature in your field, we can generalise as follows:  

Secondary sources 

Secondary sources normally include the literature (books and articles) with the experts' findings, analysis and discussions on a certain topic (Cottrell, 2014, p123). Secondary sources often interpret primary sources.  

Primary sources 

Primary sources are "first-hand" information such as raw data, statistics, interviews, surveys, law statutes and law cases. Even literary texts, pictures and films can be primary sources if they are the object of research (rather than, for example, documentaries reporting on something else, in which case they would be secondary sources). The distinction between primary and secondary sources sometimes lies on the use you make of them (Cottrell, 2014, p123). 

Primary data 

Primary data are data (primary sources) you directly obtained through your empirical work (Saunders, Lewis and Thornhill 2015, p316). 

Secondary data 

Secondary data are data (primary sources) that were originally collected by someone else (Saunders, Lewis and Thornhill 2015, p316).   

Comparison between primary and secondary data   

Use  

Virtually all research will use secondary sources, at least as background information. 

Often, especially at the postgraduate level, it will also use primary sources - secondary and/or primary data. The engagement with primary sources is generally appreciated, as less reliant on others' interpretations, and closer to 'facts'. 

The use of primary data, as opposed to secondary data, demonstrates the researcher's effort to do empirical work and find evidence to answer her specific research question and fulfill her specific research objectives. Thus, primary data contribute to the originality of the research.    

Ultimately, you should state in this section of the methodology: 

What sources and data you are using and why (how are they going to help you answer the research question and/or test the hypothesis. 

If using primary data, why you employed certain strategies to collect them. 

What the advantages and disadvantages of your strategies to collect the data (also refer to the research in you field and research methods literature). 

Quantitative, Qualitative & Mixed Methods

The methodology chapter should reference your use of quantitative research, qualitative research and/or mixed methods. The following is a description of each along with their advantages and disadvantages. 

Quantitative research 

Quantitative research uses numerical data (quantities) deriving, for example, from experiments, closed questions in surveys, questionnaires, structured interviews or published data sets (Cottrell, 2014, p93). It normally processes and analyses this data using quantitative analysis techniques like tables, graphs and statistics to explore, present and examine relationships and trends within the data (Saunders, Lewis and Thornhill, 2015, p496). 

Qualitative research  

Qualitative research is generally undertaken to study human behaviour and psyche. It uses methods like in-depth case studies, open-ended survey questions, unstructured interviews, focus groups, or unstructured observations (Cottrell, 2014, p93). The nature of the data is subjective, and also the analysis of the researcher involves a degree of subjective interpretation. Subjectivity can be controlled for in the research design, or has to be acknowledged as a feature of the research. Subject-specific books on (qualitative) research methods offer guidance on such research designs.  

Mixed methods 

Mixed-method approaches combine both qualitative and quantitative methods, and therefore combine the strengths of both types of research. Mixed methods have gained popularity in recent years.  

When undertaking mixed-methods research you can collect the qualitative and quantitative data either concurrently or sequentially. If sequentially, you can for example, start with a few semi-structured interviews, providing qualitative insights, and then design a questionnaire to obtain quantitative evidence that your qualitative findings can also apply to a wider population (Specht, 2019, p138). 

Ultimately, your methodology chapter should state: 

Whether you used quantitative research, qualitative research or mixed methods. 

Why you chose such methods (and refer to research method sources). 

Why you rejected other methods. 

How well the method served your research. 

The problems or limitations you encountered. 

Doug Specht, Senior Lecturer at the Westminster School of Media and Communication, explains mixed methods research in the following video:

LinkedIn Learning Video on Academic Research Foundations: Quantitative

The video covers the characteristics of quantitative research, and explains how to approach different parts of the research process, such as creating a solid research question and developing a literature review. He goes over the elements of a study, explains how to collect and analyze data, and shows how to present your data in written and numeric form.

Link to quantitative research video

Some Types of Methods

There are several methods you can use to get primary data. To reiterate, the choice of the methods should depend on your research question/hypothesis. 

Whatever methods you will use, you will need to consider: 

why did you choose one technique over another? What were the advantages and disadvantages of the technique you chose? 

what was the size of your sample? Who made up your sample? How did you select your sample population? Why did you choose that particular sampling strategy?) 

ethical considerations (see also tab...)  

safety considerations  

validity  

feasibility  

recording  

procedure of the research (see box procedural method...).  

Check Stella Cottrell's book  Dissertations and Project Reports: A Step by Step Guide  for some succinct yet comprehensive information on most methods (the following account draws mostly on her work). Check a research methods book in your discipline for more specific guidance.  

Experiments 

Experiments are useful to investigate cause and effect, when the variables can be tightly controlled. They can test a theory or hypothesis in controlled conditions. Experiments do not prove or disprove an hypothesis, instead they support or not support an hypothesis. When using the empirical and inductive method it is not possible to achieve conclusive results. The results may only be valid until falsified by other experiments and observations. 

For more information on Scientific Method, click here . 

Observations 

Observational methods are useful for in-depth analyses of behaviours in people, animals, organisations, events or phenomena. They can test a theory or products in real life or simulated settings. They generally a qualitative research method.  

Questionnaires and surveys 

Questionnaires and surveys are useful to gain opinions, attitudes, preferences, understandings on certain matters. They can provide quantitative data that can be collated systematically; qualitative data, if they include opportunities for open-ended responses; or both qualitative and quantitative elements. 

Interviews  

Interviews are useful to gain rich, qualitative information about individuals' experiences, attitudes or perspectives. With interviews you can follow up immediately on responses for clarification or further details. There are three main types of interviews: structured (following a strict pattern of questions, which expect short answers), semi-structured (following a list of questions, with the opportunity to follow up the answers with improvised questions), and unstructured (following a short list of broad questions, where the respondent can lead more the conversation) (Specht, 2019, p142). 

This short video on qualitative interviews discusses best practices and covers qualitative interview design, preparation and data collection methods. 

Focus groups   

In this case, a group of people (normally, 4-12) is gathered for an interview where the interviewer asks questions to such group of participants. Group interactions and discussions can be highly productive, but the researcher has to beware of the group effect, whereby certain participants and views dominate the interview (Saunders, Lewis and Thornhill 2015, p419). The researcher can try to minimise this by encouraging involvement of all participants and promoting a multiplicity of views. 

This video focuses on strategies for conducting research using focus groups.  

Check out the guidance on online focus groups by Aliaksandr Herasimenka, which is attached at the bottom of this text box. 

Case study 

Case studies are often a convenient way to narrow the focus of your research by studying how a theory or literature fares with regard to a specific person, group, organisation, event or other type of entity or phenomenon you identify. Case studies can be researched using other methods, including those described in this section. Case studies give in-depth insights on the particular reality that has been examined, but may not be representative of what happens in general, they may not be generalisable, and may not be relevant to other contexts. These limitations have to be acknowledged by the researcher.     

Content analysis 

Content analysis consists in the study of words or images within a text. In its broad definition, texts include books, articles, essays, historical documents, speeches, conversations, advertising, interviews, social media posts, films, theatre, paintings or other visuals. Content analysis can be quantitative (e.g. word frequency) or qualitative (e.g. analysing intention and implications of the communication). It can detect propaganda, identify intentions of writers, and can see differences in types of communication (Specht, 2019, p146). Check this page on collecting, cleaning and visualising Twitter data.

Extra links and resources:  

Research Methods  

A clear and comprehensive overview of research methods by Emerald Publishing. It includes: crowdsourcing as a research tool; mixed methods research; case study; discourse analysis; ground theory; repertory grid; ethnographic method and participant observation; interviews; focus group; action research; analysis of qualitative data; survey design; questionnaires; statistics; experiments; empirical research; literature review; secondary data and archival materials; data collection. 

Doing your dissertation during the COVID-19 pandemic  

Resources providing guidance on doing dissertation research during the pandemic: Online research methods; Secondary data sources; Webinars, conferences and podcasts; 

• Virtual Focus Groups Guidance on managing virtual focus groups

5 Minute Methods Videos

The following are a series of useful videos that introduce research methods in five minutes. These resources have been produced by lecturers and students with the University of Westminster's School of Media and Communication. 

Case Study Research

Research Ethics

Quantitative Content Analysis 

Sequential Analysis 

Qualitative Content Analysis 

Thematic Analysis 

Social Media Research 

Mixed Method Research 

Procedural Method

In this part, provide an accurate, detailed account of the methods and procedures that were used in the study or the experiment (if applicable!). 

Include specifics about participants, sample, materials, design and methods. 

If the research involves human subjects, then include a detailed description of who and how many participated along with how the participants were selected.  

Describe all materials used for the study, including equipment, written materials and testing instruments. 

Identify the study's design and any variables or controls employed. 

Write out the steps in the order that they were completed. 

Indicate what participants were asked to do, how measurements were taken and any calculations made to raw data collected. 

Specify statistical techniques applied to the data to reach your conclusions. 

Provide evidence that you incorporated rigor into your research. This is the quality of being thorough and accurate and considers the logic behind your research design. 

Highlight any drawbacks that may have limited your ability to conduct your research thoroughly. 

You have to provide details to allow others to replicate the experiment and/or verify the data, to test the validity of the research. 

Bibliography

Cottrell, S. (2014). Dissertations and project reports: a step by step guide. Hampshire, England: Palgrave Macmillan.

Lombard, E. (2010). Primary and secondary sources.  The Journal of Academic Librarianship , 36(3), 250-253

Saunders, M.N.K., Lewis, P. and Thornhill, A. (2015).  Research Methods for Business Students.  New York: Pearson Education. 

Specht, D. (2019).  The Media And Communications Study Skills Student Guide . London: University of Westminster Press.  

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How To Write Methodology For A Quantitative Study?

Methodology Chapter Provides Information On All The Steps Taken For The Study Of A Problem And The Justifications Given For Specific Steps Taken To Gather, Process, And Analyze Data Related To Understanding The Problem. The Chapter Allows The Reader To Understand And Evaluate The Research Design And Thus Validate It.

Quantitative Study: Quantitative Research Is The Quantification Of A Problem Via Generating Numerical Data That Are Most Often Converted Into Statistics. The Statistical Analysis In Research Methodology Helps In Proving Or Disproving A Thesis. The Research Is Usually Conducted Among A Broad Populace In The Form Of Questionnaire, Polls And Other Surveys Which Involves The Participants To Reply In Numbers. This Means Quantitative Research Falls Under The Headings Of Empirical Or Statistical Studies. Its Main Characteristics Are :

• Use Of Standard Instruments To Gather Data.
• Sizes Of The Samples Are Proportional To That Of The Population In Question.
• Research Is Conducted On A Well-Defined Question.
• Studies Are Well Designed Before The Process.
• Numerical Data That Quantify A Problem Or An Area Of Research Using Various Means Like Charts, Tables, Graphs, Etc. Data Collection Carried Out Through Surveys, Questionnaires, Etc.

A Methodology Chapter For A Quantitative Study Explains The Following In A Direct And Precise Manner:

How Was The Data Generated? How Was It Processed? Why Was The Design, That Was Used, Used?

Any Methodology Chapter Must Be Written In The Past Tense And Follows The Following Guidelines: Chapter Provides Information On The Methods Of Data Collection To Enable The Readers To Understand The Process.

• Significance Of The Methodology Is That A Proper Methodology Validates The Study As A Whole.
• Methodology Section Should Provide A Rationale For Choosing A Particular Design.
• Methods Used Must Be Valid And Conforming To The Conventional Norms Of Research. As Such, The Methodology Chapter Must Provide Enough Evidence To Support The Credibility Of Each Method Used.
• The Appropriateness Of The Methods Chosen Must Be Stated. Only The Methods That Are Appropriate To The Scale Of The Subject In Investigation Must Be Chosen.
• The Chapter Must Contain Information On The Constraints And Challenges That Were Anticipated Before The Beginning Of The Study, The Steps Taken To Prevent The Problems From Occurring, And The Ways The Constraints That Did Occur Were Tackled With Minimal Impact And How The Said Impact Doesn’t Affect The Outcome Of The Study.
• Necessary Information To Replicate Any Particular Method Must Be Provided Concisely In The Chapter.

A methodology for a quantitative study begins with a reiteration of the research question and its context.

This is followed by the research design , where the methods used to gather, process and analyze the data are given. This is usually preceded or followed by a justification of the appropriateness of the research design . Then, the information on the pilot study is given. It is followed by the precise information on sampling strategies, instrument design, and methods of data analysis . ‘ Ethical considerations ’ comes next . Here the researcher informs the reader of the measures taken to ensure participants privacy and consent, etc. Penultimate to concluding the methodology chapter is problems and limitations, where the reader is informed of all the constraints the researcher anticipated and all the problems that occurred and the limitations methods may pose to the overall study.

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9 Quantitative Research Methods With Real Client Examples

• June 21, 2021
• Tallwave Team

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Quantitative research is essential to developing a clear understanding of consumer engagement and how to increase satisfaction.

Primary Quantitative Research Methods

When it comes to quantitative research, many people often confuse this type of research with the methodology. The research type refers to style of research while the data collection method can be different.

Research types

These are the primary types of quantitative research used by businesses today.

• Survey research: Ideally when conducting survey research businesses will use a statistically relevant sample to understand the sentiments and actions of a large group of people. This could be their current customers or consumers who fit into their ideal demographic.
• Correlational research: Correlational research compares two variables to come to a conclusion about whether there is a relationship between the two. Keep in mind that correlation does not always imply causation, which is to say you need to account for external variables that could cause an apparent relationship.
• Experimental research: This form of research takes a scientific approach, testing a hypothesis by manipulating certain variables to understand what changes this could cause. In these experiments, there is a control group and a manipulated group.

Also read:  6 Factors Influencing Customer Behaviors in 2021

Data collection methods

Launching the above research requires creating a plan to collect data. After all, quantitative research relies on data. Here are the common primary data collection methods for quantitative research.

• Surveys: A common approach to collecting data is using a survey. This is ideal especially if the business can obtain a statistically relevant sample from their responses. Surveys are often conducted through web or email questionnaires.
• Interviews: Yes, interviews can be used to obtain quantitative data. While this form of data collection is typically associated with qualitative research, interviewers can ask a standard set of questions to collate formal, quantitative data.
• Documentation review: With an increasing amount of business occurring digitally, there is more documentation now than ever before to help inform quantitative conclusions. Businesses can assess website metrics such as return visits, time on page or even use a pixel to track customer movement across websites. They can also view how many times their app has been opened and actions users have taken on their platform to determine customer engagement.

Secondary research can be helpful when formulating a plan for obtaining primary quantitative data. It can help narrow areas of focus or illuminate key challenges.

Secondary Quantitative Research Methods

Secondary data is information that is already collected and not necessarily exclusive to the company but still relevant when understanding overall industry and marketplace trends. Here are a few examples of secondary data:

• Government reports: Government research can indicate potential regulatory roadblocks, customer pain points and future opportunities. For example, a fitness company might use government data that shows an increase in use of outdoor running trials to develop a new product used to meet that specific use case.
• Survey-based secondary data: Polls or surveys that have been conducted for a primary use could be reused for secondary purposes. This could include survey data obtained by other companies or governments.
• Academic research: Research that has been previously conducted and published in peer-reviewed journals can help inform trends and consumer behavior, even if it doesn’t apply to a company’s specific customers.

Secondary research can be helpful when formulating a plan for obtaining primary quantitative data. It can help narrow areas of focus or illuminate key challenges. It can also help when it comes to interpreting primary data, especially when trying to understand the relationship between two variables of correlated data.

Also read:  The What, Why, & How of Customer Behavior Analysis

Real Examples of Quantitative Research

We regularly use quantitative research to help our clients understand where they can best add value to increase customer engagement. Here are three examples of quantitative research in motion.

Example 1: Leading food distribution company

We helped a leading food distribution company identify changes in the needs and values of their restaurant clients as a result of COVID-19. This helped inform opportunities to become more valuable partners.

The research plan involved creating a survey that was emailed to clients. The questions were specific and numeric. For example, respondents were asked what percentage of their weekly spend was used with the food distribution company. They were also asked to assign a percentage to the way their food ordering had changed during COVID-19 and to rate their satisfaction with the food distribution company.

The results showed changes that had occurred for clients of the food distribution company as a result of the unique stressors of the pandemic. We were able to determine changes in weekly food supply and customer count as well as menu adaptations and purchase behavior.

Example 2: Leading credit card company

Our work with a leading credit card company required us to understand what current travel card members valued about the rewards program and their preferred communication method for booking travel in order to create an omnichannel servicing strategy and ideal customer journey.

Through an online survey of younger cardholders, the target demographic for this project, we asked questions such as length of card membership, total spend and the number of annual leisure trips in addition to more specific questions that showed how members get inspiration for trip planning and where they research.

The results highlighted ways to overcome resistance to pricing by proving more value. It also illuminated ways to make the benefits of membership more tangible to card holders and how to influence travelers in the early stages of planning their journey.

Example 3: Internal research report

We’re in the business of drinking our own champagne, so to speak, which is why we conducted our own quantitative research aimed at understanding the consumer trends that were spurred by the pandemic and how these will transform behaviors in the future.

There’s no question that new customer experiences emerged from the pandemic. Think of offerings such as “buy online, pickup in store (BOPIS),” or blended restaurant meals that are cooked at home. We wanted to understand how consumers truly felt about these new experiences and which they were likely to continue using even after restrictions were lifted. We also wanted to know more about the changing expectations for branded communication and how all of these pieces of the puzzle fit together to create consumer engagement. Our method of data collection was a survey.

Our research led us to develop insights we could use to inform our customers in their decision making. For example, we found convenience is paramount for consumers who are seeking out hybrid experiences such as BOPIS to take the best of both worlds. We also found many of these changes are permanent as consumers embraced new experiences that made their lives easier.

We regularly use quantitative research to help our clients understand where they can best add value to increase customer engagement.

The Bottom Line

Quantitative research is essential to developing a clear understanding of consumer engagement and how to increase satisfaction. Though online surveys are one of the most common methods for obtaining data, research isn’t limited to this strategy. It’s important to use whatever strategies are within your scope to constantly evaluate new trends and consumer behaviors that could significantly impact your offerings. The results can show you how to re-engage customers and drive loyalty.

Interested in partnering with us to learn more about your customers needs, wants, and behaviors to inform future experience design? Contact us today !

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• What Is a Research Methodology? | Steps & Tips

What Is a Research Methodology? | Steps & Tips

Published on August 25, 2022 by Shona McCombes and Tegan George. Revised on November 20, 2023.

Your research methodology discusses and explains the data collection and analysis methods you used in your research. A key part of your thesis, dissertation , or research paper , the methodology chapter explains what you did and how you did it, allowing readers to evaluate the reliability and validity of your research and your dissertation topic .

It should include:

• The type of research you conducted
• How you collected and analyzed your data
• Any tools or materials you used in the research
• How you mitigated or avoided research biases
• Why you chose these methods
• Your methodology section should generally be written in the past tense .
• Academic style guides in your field may provide detailed guidelines on what to include for different types of studies.
• Your citation style might provide guidelines for your methodology section (e.g., an APA Style methods section ).

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Table of contents

How to write a research methodology, why is a methods section important, step 1: explain your methodological approach, step 2: describe your data collection methods, step 3: describe your analysis method, step 4: evaluate and justify the methodological choices you made, tips for writing a strong methodology chapter, other interesting articles, frequently asked questions about methodology.

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Your methods section is your opportunity to share how you conducted your research and why you chose the methods you chose. It’s also the place to show that your research was rigorously conducted and can be replicated .

It gives your research legitimacy and situates it within your field, and also gives your readers a place to refer to if they have any questions or critiques in other sections.

You can start by introducing your overall approach to your research. You have two options here.

Option 1: Start with your “what”

What research problem or question did you investigate?

• Aim to describe the characteristics of something?
• Explore an under-researched topic?
• Establish a causal relationship?

And what type of data did you need to achieve this aim?

• Quantitative data , qualitative data , or a mix of both?
• Primary data collected yourself, or secondary data collected by someone else?
• Experimental data gathered by controlling and manipulating variables, or descriptive data gathered via observations?

Option 2: Start with your “why”

Depending on your discipline, you can also start with a discussion of the rationale and assumptions underpinning your methodology. In other words, why did you choose these methods for your study?

• Why is this the best way to answer your research question?
• Is this a standard methodology in your field, or does it require justification?
• Were there any ethical considerations involved in your choices?
• What are the criteria for validity and reliability in this type of research ? How did you prevent bias from affecting your data?

Once you have introduced your reader to your methodological approach, you should share full details about your data collection methods .

Quantitative methods

In order to be considered generalizable, you should describe quantitative research methods in enough detail for another researcher to replicate your study.

Here, explain how you operationalized your concepts and measured your variables. Discuss your sampling method or inclusion and exclusion criteria , as well as any tools, procedures, and materials you used to gather your data.

Surveys Describe where, when, and how the survey was conducted.

• How did you design the questionnaire?
• What form did your questions take (e.g., multiple choice, Likert scale )?
• Were your surveys conducted in-person or virtually?
• What sampling method did you use to select participants?
• What was your sample size and response rate?

Experiments Share full details of the tools, techniques, and procedures you used to conduct your experiment.

• How did you design the experiment ?
• How did you recruit participants?
• How did you manipulate and measure the variables ?
• What tools did you use?

Existing data Explain how you gathered and selected the material (such as datasets or archival data) that you used in your analysis.

• Where did you source the material?
• How was the data originally produced?
• What criteria did you use to select material (e.g., date range)?

The survey consisted of 5 multiple-choice questions and 10 questions measured on a 7-point Likert scale.

The goal was to collect survey responses from 350 customers visiting the fitness apparel company’s brick-and-mortar location in Boston on July 4–8, 2022, between 11:00 and 15:00.

Here, a customer was defined as a person who had purchased a product from the company on the day they took the survey. Participants were given 5 minutes to fill in the survey anonymously. In total, 408 customers responded, but not all surveys were fully completed. Due to this, 371 survey results were included in the analysis.

• Information bias
• Omitted variable bias
• Regression to the mean
• Survivorship bias
• Undercoverage bias
• Sampling bias

Qualitative methods

In qualitative research , methods are often more flexible and subjective. For this reason, it’s crucial to robustly explain the methodology choices you made.

Be sure to discuss the criteria you used to select your data, the context in which your research was conducted, and the role you played in collecting your data (e.g., were you an active participant, or a passive observer?)

Interviews or focus groups Describe where, when, and how the interviews were conducted.

• How did you find and select participants?
• How many participants took part?
• What form did the interviews take ( structured , semi-structured , or unstructured )?
• How long were the interviews?
• How were they recorded?

Participant observation Describe where, when, and how you conducted the observation or ethnography .

• What group or community did you observe? How long did you spend there?
• How did you gain access to this group? What role did you play in the community?
• How long did you spend conducting the research? Where was it located?
• How did you record your data (e.g., audiovisual recordings, note-taking)?

Existing data Explain how you selected case study materials for your analysis.

• What type of materials did you analyze?
• How did you select them?

In order to gain better insight into possibilities for future improvement of the fitness store’s product range, semi-structured interviews were conducted with 8 returning customers.

Here, a returning customer was defined as someone who usually bought products at least twice a week from the store.

Surveys were used to select participants. Interviews were conducted in a small office next to the cash register and lasted approximately 20 minutes each. Answers were recorded by note-taking, and seven interviews were also filmed with consent. One interviewee preferred not to be filmed.

• The Hawthorne effect
• Observer bias
• The placebo effect
• Response bias and Nonresponse bias
• The Pygmalion effect
• Recall bias
• Social desirability bias
• Self-selection bias

Mixed methods

Mixed methods research combines quantitative and qualitative approaches. If a standalone quantitative or qualitative study is insufficient to answer your research question, mixed methods may be a good fit for you.

Mixed methods are less common than standalone analyses, largely because they require a great deal of effort to pull off successfully. If you choose to pursue mixed methods, it’s especially important to robustly justify your methods.

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Next, you should indicate how you processed and analyzed your data. Avoid going into too much detail: you should not start introducing or discussing any of your results at this stage.

In quantitative research , your analysis will be based on numbers. In your methods section, you can include:

• How you prepared the data before analyzing it (e.g., checking for missing data , removing outliers , transforming variables)
• Which software you used (e.g., SPSS, Stata or R)
• Which statistical tests you used (e.g., two-tailed t test , simple linear regression )

In qualitative research, your analysis will be based on language, images, and observations (often involving some form of textual analysis ).

Specific methods might include:

• Content analysis : Categorizing and discussing the meaning of words, phrases and sentences
• Thematic analysis : Coding and closely examining the data to identify broad themes and patterns
• Discourse analysis : Studying communication and meaning in relation to their social context

Mixed methods combine the above two research methods, integrating both qualitative and quantitative approaches into one coherent analytical process.

Above all, your methodology section should clearly make the case for why you chose the methods you did. This is especially true if you did not take the most standard approach to your topic. In this case, discuss why other methods were not suitable for your objectives, and show how this approach contributes new knowledge or understanding.

In any case, it should be overwhelmingly clear to your reader that you set yourself up for success in terms of your methodology’s design. Show how your methods should lead to results that are valid and reliable, while leaving the analysis of the meaning, importance, and relevance of your results for your discussion section .

• Quantitative: Lab-based experiments cannot always accurately simulate real-life situations and behaviors, but they are effective for testing causal relationships between variables .
• Qualitative: Unstructured interviews usually produce results that cannot be generalized beyond the sample group , but they provide a more in-depth understanding of participants’ perceptions, motivations, and emotions.
• Mixed methods: Despite issues systematically comparing differing types of data, a solely quantitative study would not sufficiently incorporate the lived experience of each participant, while a solely qualitative study would be insufficiently generalizable.

Remember that your aim is not just to describe your methods, but to show how and why you applied them. Again, it’s critical to demonstrate that your research was rigorously conducted and can be replicated.

1. Focus on your objectives and research questions

The methodology section should clearly show why your methods suit your objectives and convince the reader that you chose the best possible approach to answering your problem statement and research questions .

2. Cite relevant sources

Your methodology can be strengthened by referencing existing research in your field. This can help you to:

• Show that you followed established practice for your type of research
• Discuss how you decided on your approach by evaluating existing research
• Present a novel methodological approach to address a gap in the literature

3. Write for your audience

Consider how much information you need to give, and avoid getting too lengthy. If you are using methods that are standard for your discipline, you probably don’t need to give a lot of background or justification.

Regardless, your methodology should be a clear, well-structured text that makes an argument for your approach, not just a list of technical details and procedures.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Measures of central tendency
• Chi square tests
• Confidence interval
• Quartiles & Quantiles

Methodology

• Cluster sampling
• Stratified sampling
• Thematic analysis
• Cohort study
• Peer review
• Ethnography

Research bias

• Implicit bias
• Cognitive bias
• Conformity bias
• Hawthorne effect
• Availability heuristic
• Attrition bias

Methodology refers to the overarching strategy and rationale of your research project . It involves studying the methods used in your field and the theories or principles behind them, in order to develop an approach that matches your objectives.

Methods are the specific tools and procedures you use to collect and analyze data (for example, experiments, surveys , and statistical tests ).

In shorter scientific papers, where the aim is to report the findings of a specific study, you might simply describe what you did in a methods section .

In a longer or more complex research project, such as a thesis or dissertation , you will probably include a methodology section , where you explain your approach to answering the research questions and cite relevant sources to support your choice of methods.

In a scientific paper, the methodology always comes after the introduction and before the results , discussion and conclusion . The same basic structure also applies to a thesis, dissertation , or research proposal .

Depending on the length and type of document, you might also include a literature review or theoretical framework before the methodology.

Quantitative research deals with numbers and statistics, while qualitative research deals with words and meanings.

Quantitative methods allow you to systematically measure variables and test hypotheses . Qualitative methods allow you to explore concepts and experiences in more detail.

Reliability and validity are both about how well a method measures something:

• Reliability refers to the  consistency of a measure (whether the results can be reproduced under the same conditions).
• Validity   refers to the  accuracy of a measure (whether the results really do represent what they are supposed to measure).

If you are doing experimental research, you also have to consider the internal and external validity of your experiment.

A sample is a subset of individuals from a larger population . Sampling means selecting the group that you will actually collect data from in your research. For example, if you are researching the opinions of students in your university, you could survey a sample of 100 students.

In statistics, sampling allows you to test a hypothesis about the characteristics of a population.

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• Open access
• Published: 07 March 2024

Quantifying carboxymethyl lysine and carboxyethyl lysine in human plasma: clinical insights into aging research using liquid chromatography-tandem mass spectrometry

• Daguang Wang 1 ,
• Junshan Wang 2 ,
• Xinghong Liu 2 ,
• Kehe Du 2 ,
• Hongjun Liu 2 ,
• Xiaofeng Yang 3 ,
• Tianyi Liu 3 ,
• Qian Liu 3 ,
• Meng Wang 3 &
• Jian Guo 3  

BMC Biotechnology volume  24 , Article number:  12 ( 2024 ) Cite this article

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Metrics details

The objective of this study was to establish a methodology for determining carboxymethyl lysine (CML) and carboxyethyl lysine (CEL) concentrations in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The test results were also used for clinical aging research.

Human plasma samples were incubated with aqueous perfluorovaleric acid (NFPA), succeeded by precipitation utilizing trichloroacetic acid, hydrolysis facilitated by hydrochloric acid, nitrogen drying, and ultimate re-dissolution utilizing NFPA, followed by filtration. Cotinine-D 3 was added as an internal standard. The separation was performed on an Agela Venusil ASB C 18 column (50 mm × 4.6 mm, 5 μm) with a 5 mmol/L NFPA and acetonitrile/water of 60:40 (v/v) containing 0.15% formic acid. The multiple reaction monitoring mode was used for detecting CML, CEL, and cotinine-D 3 , with ion pairs m/z 205.2 > 84.1 (for quantitative) and m/z 205.2 > m/z 130.0 for CML, m/z 219.1 > 84.1 (for quantitative) and m/z 219.1 > m/z 130.1 for CEL, and m/z 180.1 > 80.1 for cotinine-D 3 , respectively.

The separation of CML and CEL was accomplished within a total analysis time of 6 minutes. The retention times of CML, CEL, and cotinine-D 3 were 3.43 minutes, 3.46 minutes, and 4.50 minutes, respectively. The assay exhibited linearity in the concentration range of 0.025–1.500 μmol/L, with a lower limit of quantification of 0.025 μmol/L for both compounds. The relative standard deviations of intra-day and inter-day were both below 9%, and the relative errors were both within the range of ±4%. The average recoveries were 94.24% for CML and 97.89% for CEL.

The results indicate that the developed methodology is fast, highly sensitive, highly specific, reproducible, and suitable for the rapid detection of CML and CEL in clinical human plasma samples. The outcomes of the clinical research project on aging underscored the important indicative significance of these two indicators for research on human aging.

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Introduction

The term Advanced Glycation End-products (AGEs) refers to a category of stable end-products resulting from a series of reactions such as condensation, rearrangement, cleavage, and oxidative modification that occur between the carbonyl groups of reducing sugars and the free amino groups of substances such as proteins, lipids, amino acids, or nucleic acids [ 1 ]. Medical studies have shown that AGEs are generated and accumulate in serum and tissues as individuals age. They are involved in the onset and progression of a variety of diseases and aging, including the development of chronic complications associated with diabetes, atherosclerosis, uremia, Alzheimer’s disease, and cataracts [ 2 , 3 , 4 ].

In order to comprehensively assess the impact of AGEs on human health, it is imperative to employ accurate and reliable methods for the measurement of appropriate biomarkers. CML, CEL, pyrraline, and pentosidine are among the over 20 AGEs that have been identified [ 5 ]. CEL and CML, in particular, are closely associated with aging as well as diseases such as diabetic complications and renal failure [ 6 , 7 , 8 , 9 ]. The development of an efficient and accurate quantitative method for the determination of CML and CEL assumes much significance for advancing further clinical research on the correlation between CML, CEL, and the aging process, as well as for assessing overall human health status.

Commonly employed methods for the determination of CML and CEL in biological samples include HPLC-fluorescence detection (FLD) with pre-column derivatization using ortho-phtaldialdehyde (OPA) [ 10 ] or 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) [ 11 ], gas chromatography-mass spectrometry (GC-MS) method by analyzing N(O,S)-ethoxycarbonyl ethyl esters of amino acid derivatized by ethylchloroformate [ 12 ], enzyme-linked immunochemical quantification of CML with a specific antibody, [ 9 , 13 ] and the HPLC with stable-isotope-dilution tandem mass spectrometry method [ 14 , 15 , 16 ], among others. Among these, HPLC-MS/MS has obvious advantages because the use of multiple reaction monitoring (MRM) mode improves sensitivity, reduces the coefficient of variability, eliminates the problems of derivatization, and allows for the simultaneous analysis of multiple analytes.

In this experiment, an efficient, sensitive, accurate, and specific technique was developed utilizing LC-MS/MS for the accurate determination of CML and CEL in human plasma.

Materials and methods

Samples, standard materials, and reagents, standard materials and samples.

CML (95% purity) and CEL (96% purity) were purchased from TMRM (China). Cotinine-D 3 (99% purity) was bought from Cerilliant (USA). A total of 1196 clinical plasma samples were provided by the Institute of Geriatrics, Beijing Hospital, China.

Methanol, acetonitrile, and formic acid, all of chromatography grade, were sourced from Burdick & Jackson (USA). The ultra-pure water that was utilized in the experiments was prepared using the Milli-Q water purification system. The NFPA used was of analytical reagent quality and obtained from Macklin (China). Trichloroacetic acid (TCA) and hydrochloric acid, both analytical reagents, were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). Additionally, 4% bovine serum albumin prepared with saline was utilized.

Detection methods

A triple quadruple mass spectrometer (MS), API 4000 (Applied Biosystems, USA), coupled to a LC system manufactured by Shimadzu (Japan) was utilized. The MS was operated in a positive ion mode using electrospray ionization (ESI) on a turbo-ion spray source. Multiple reaction monitoring (MRM) was used for the MS analyses. Ion pairs selected for analysis were m/z 205.2 > m/z 84.1 (for quantitative) and m/z 205.2 → m/z 130.0 for CML, m/z 219.1 > m/z 84.1 (for quantitative) and m/z 219.1 > m/z 130.1 for CEL, and m/z 180.1 > m/z 80.1 for cotinine-D 3 , respectively. The temperature of the auxiliary gas was set to 550 °C, the ion spray voltage was 5500 V, and the de-clustering potentials were 56 V, 56 V, 25 V, 25 V, and 64 V, respectively. Collision energies (CE) were set at 17 eV, 27 eV, 27 eV, 18 eV, and 30 eV, respectively. Nitrogen was used as the curtain, turbo-ion spray, collision, and nebulizer gas in the system.

Analyst 1.4.2 software was used for all data acquisition and processing in this study. The HPLC separation was performed using a chromatographic column (Lightn AQ 4.6 × 50 mm, 5-μm particles; Venusil ASB C 18 , Agela, USA). The composition of the solution was as follows: eluent A, 5 mmol/L NFPA, and eluent B, acetonitrile/water (v/v) = 6:4 (containing 0.15% formic acid). Eluent B (5%) underwent a linear gradient increase from 5 to 25% over 0.50 minutes, followed by a linear gradient increase from 25 to 60% over 3.51 minutes, and from 60 to 98% over 0.99 minutes. Subsequently, Eluent B returned to 5% over 0.01 minutes and remained at 5% for 1 minute. The flow rate was 1.00 mL/min, and the injection volume was 10 μL.

Standard curves and quality control (QC) samples

The composite standard curve samples for CML and CEL consisted of standard curve samples spiked with incremental concentrations of working standard solution of 0.250, 0.500, 1.000, 3.000, 4.500, 7.000, 12.000, and 15.000 μmol/L. These samples were prepared with 4% bovine serum albumin as the matrix and were tailored for specific requirements. The mixed QC samples for CML and CEL comprised QC samples spiked with serial concentrations of QC working standard solution of 1.500, 4.500, and 10.000 μmol/L formulated with 4% bovine serum albumin as the matrix. These were prepared as required.

Plasma sample processing

The analytical procedure involved pipetting 50 μL of the plasma sample to be analyzed or the blank sample into a 10 mL centrifuge tube with a screw cap. Next, 100 μL of deionized water and 500 μL of 5 mmol/L NFPA were added, and the mixture was incubated at room temperature for 2 hours. After that, 2 mL of TCA with a concentration of 200 g/L was added, and the sample was centrifuged at 2000 g for 10 minutes. The supernatant was removed, and the remaining solution was washed with 1 mL of TCA with a concentration of 100 g/L. The supernatant was washed by adding 1 mL of 100 g/L TCA, centrifuged at 2000 g for 10 minutes, and removed. The remaining solution was added to 500 μL of a 6 mol/L hydrochloric acid solution and subjected to hydrolysis in an oven at 110 °C for 20 hours. The hydrolyzed product was dried using nitrogen gas in a water bath at 80 °C, then added to 300 μL of NFPA (5 mmol/L). The remaining solution was drawn using a 1 mL syringe and filtered through a 0.22 μm membrane. A 200-μL aliquot of the membrane-filtered solution was taken and mixed well with 20 μL of Cotinine-D 3 (500 ng/mL, prepared with 50% acetonitrile-water). Finally, the resulting mixture was subjected to LC-MS/MS analysis.

Data collection and processing

The chromatograms of CML, CEL, and Cotinine-D 3 were integrated using the Analyst version 1.4.2 software. Linear regression was performed using the area ratio of CML or CEL to Cotinine-D 3 as the vertical coordinate and the corresponding concentration of CML or CEL as the horizontal coordinate, with a weighting coefficient of 1/x 2 . The concentration of CML or CEL in the unknown samples was calculated using the following formula:

Wherein, y represents the ratio of the peak area of CML or CEL to that of its internal standard Cotinine-D 3 ; b is the intercept of the standard curve; and a is the slope of the standard curve.

Methodological validation

Standard curve and lower limit of quantification (lloq).

For determination of the standard curve and lower limit of quantification (LLOQ), 20 μL of each standard sample with varying concentrations was combined with 20 μL of Cotinine-D 3 (500 ng/mL, prepared with 50% acetonitrile water) in a 1.5 mL microcentrifuge tube and then added 180 μL of a 5-mmol/L NFPA aqueous solution. This was mixed well and then analyzed using LC-MS/MS. Each analytical batch incorporated one standard curve, and five consecutive analytical batches were analyzed. The results are shown in Table  1 , and the typical working curves of CML and CEL are shown in Fig.  1 -1 (correlation coefficient R 2  = 0.9886) and Fig. 1 -2 (correlation coefficient R 2  = 0.9920), respectively.

1-1: Standard curve of CML in human plasma. 1-2: Standard curve of CEL in human plasma

Figure 2 -1 and 2-2 illustrate the LLOQ chromatograms of CML and CEL, respectively. The signal-to-noise ratio, as calculated by the software, was greater than 10, and the LLOQ of CML and CEL were both 0.025 μmol/L, thereby meeting the established criteria for quantitative detection.

2-1: LLOQ of CML in human plasma. 2-2: LLOQ of CEL in human plasma

Accuracy and precision

The QC samples were processed with low, moderate, and high concentrations of CML and CEL as per the procedure outlined in Section Standard curve and lower limit of quantification (LLOQ). The concentrations of QC samples were measured with the corresponding working curve of the analytical batch, and the accuracy and precision of the method were calculated based on the measured results of the QC samples. The results are shown in Table  2 . The relative errors (REs) were all within ±15%, and the intra-batch relative standard deviation (RSD) and inter-batch RSD were both less than 15%, satisfying the stipulated requirements for accuracy and precision.

Extraction recoveries

The QC samples of CML and CEL with low, moderate, and high concentrations were processed as per the procedure detailed in Section Standard curve and lower limit of quantification (LLOQ). Three parallel samples were set up for each sample, which were then analyzed using LC-MS/MS to obtain the peak areas of CML and CEL. A volume of 50 μL from the blank matrix (comprising 4% bovine serum albumin) was dispensed and subjected to the protocol outlined in Section Plasma sample processing, designated “Plasma Sample Processing.” It was blown dry with nitrogen, and then 10 μL of a mixture of CML and CEL with the corresponding concentration were added to 290 μL of NFPA (5 mmol/L).

Following the remaining steps specified in the section on “Plasma Sample Processing”, three parallel samples were set up for each sample and analyzed using LC-MS/MS to obtain the peak areas of CML and CEL. At each concentration, the average peak area of CML and CEL obtained by the previous treatment method was divided by the average peak area of CML and CEL obtained by the latter treatment method to calculate the extraction recovery rates. The extraction recoveries of CML were 89.16, 92.91, and 100.64% for low, medium, and high concentration plasma samples, respectively, with an average extraction recovery of 94.24%, while it was 97.63, 97.48, and 98.57% for CEL, respectively, with an average extraction recovery of 97.89%. The results are shown in Table  3 .

Specificity

For specificity evaluation, 50 μL of 4% bovine serum albumin was employed, and an equivalent volume of 50% acetonitrile water was substituted for the internal standard solution. The methodology outlined in the “Plasma Sample Processing” section was then adhered to for the generation of MRM chromatograms pertaining to the blank samples (refer to Fig. 3 -1 and 3-2). CML, CEL, and cotinine-D3 internal standards were introduced into 4% bovine serum albumin samples. Subsequently, the procedures were conducted in accordance with the protocol delineated in the section titled “Plasma Sample Processing,” resulting in the acquisition of MRM chromatograms for the spiked samples (Fig. 3 -3 and 3-4). Actual plasma samples were subjected to the procedures described in the “Plasma Sample Processing” section to obtain the MRM chromatograms of the actual samples (Fig. 3 -5 and 3-6). The results showed that the endogenous substances in 4% bovine serum albumin did not interfere with the determination of CML, CEL, or the internal standard. Hence, the method exhibited good specificity.

3-1: Chromatogram of CML in 4% bovine serum albumin. 3-2: Chromatogram of CEL in 4% bovine serum albumin. 3-3: Chromatogram of CML and IS in 4% bovine serum albumin. 3-4: Chromatogram of CEL and IS in 4% bovine serum albumin. 3-5: Chromatogram of CML and IS in 4% bovine serum albumin. 3-6: Chromatogram of CEL and IS in 4% bovine serum albumin

Use of the method in clinical samples

The clinical protocols used in this study were approved by the ethics committee of the institution, and the principles outlined in the Declaration of Helsinki were complied with. Informed consent was obtained from all study participants. A comprehensive analysis was conducted on a total of 1196 clinical plasma samples, which were provided by the Institute of Geriatrics at Beijing Hospital, China. The primary objective of this study was to investigate the variations in CML and CEL levels in relation to advancing age.

Comparison of CML and CEL between different age groups

The samples were categorized into the following four groups based on the age of the study participants: the youth group: ≤ 44 years old; the middle-aged group: 45–59 years old; the elderly group: 60–74 years old; and the advanced age group: ≥ 75 years old. Analysis of variance (ANOVA) between groups was performed to examine the differences in CML and CEL between these age-based groups. As there were some differences in the indicators for male and female participants, separate ANOVA analyses were done for men and women. Results are shown in Table  4 .

The results indicate significant differences in CML and CEL between the various age-based groups in the male cohort. In the female cohort, there was a significant difference between the different groups only in CEL levels.

Correlation analyses of CML and CEL with age

Pearson’s correlation coefficients for CML and CEL in relation to age were computed separately for each gender, and the corresponding findings are presented in Table  5 .

For the correlation between results and aging, male correlation is higher than female correlation for CML, female correlation is higher than male correlation for CEL.

Analyses of the degree of change in CML and CEL with aging

The samples were stratified based on 10-year age intervals. Given the limited number of individuals aged over 90 years, they were aggregated with the age group of 80 years and above. Consequently, the ultimate analysis comprised a total of seven distinct age groups. The mean values and variances of the indicators were computed for each age group. Subsequently, the curves depicting the mean changes for each gender were graphically represented and overlaid on the same chart. Notably, the results showed that the change rate of CML and CEL test results with age was greater in male than in female. (Fig. 4 -1 and 4-2).

4-1: Changes in CML values with increasing age. 4-2: Changes in CEL values with increasing age

Quantification of the degree of change in CML and CEL

In order to quantify the trends in the alterations of CML and CEL with age, percentage increases (or decreases) for each biomarker were computed over 10-year intervals. Notably, it was observed that the average change in CML and CEL levels every 10 years remained inconspicuous below the age of 40, generally registering below 5%. A distinct upward trend became evident after the age of 40. Consequently, for the age group exceeding 40 years, the average magnitudes of change in the two indicators were recalculated every 10 years. The outcomes indicated a noteworthy increase in the respective degrees of change for CML and CEL, as elucidated in Table  6 .

This preliminary determination of the respective degrees of change of CML and CEL with age has considerable significance in guiding the development of a new laboratory assessment system for human health status. Such a system can track the physical status of the group, improve the human health status monitoring network, and promote the establishment of a system for the analysis and management of dynamic data pertaining to monitoring human health.

CML and CEL are two lysine derivatives with potential clinical significance in research on aging. Levels of CML, a metabolite associated with aging, tend to be generally low in the elderly. Studies have shown that CML expression is associated with a variety of age-related diseases and physiological processes, such as cardiovascular disease [ 17 ], chronic inflammation [ 18 ], and decreased brain function [ 19 ]. As a biomarker, CML may be useful in predicting disease risk and assessing health status in the elderly.

CEL is one of the AGEs that is formed by the non-enzymatic glycosylation of glucose or other reducing sugars with lysines in proteins. Studies have shown that CEL levels can be used to assess the extent of the glycosylation reaction and the degree of glycosylation of proteins [ 20 ]. Elevated levels of CEL have been linked to a variety of diseases, particularly diabetes and vascular disease. The accumulation of CEL due to hyperglycemia and increased glycosylation reactions can trigger inflammatory responses and oxidative stress, thereby promoting disease progression. Reports on the impact of CEL on cellular genome damage indicate its potential detrimental effects on the genomic integrity of cells [ 21 ]. Cellular experiments have shown that the presence of CEL can lead to DNA damage and chromosomal aberrations [ 21 ], which in turn increase the risk of cellular genotoxicity and mutation, among others.

In this study, we developed an analytical method using LC-MS/MS for the quantification of CML and CEL in human plasma samples. The method exhibited good selectivity, high sensitivity, and high throughput. Optimized chromatographic conditions significantly reduced the detection time, with only 6 minutes required for the simultaneous detection of both components in each sample. This contributed to improving working efficiency. This method requires only a small volume of plasma (50 μL). The method achieved an LLOQ of 0.025 μmol/L, significantly improving the detection rate of CML and CEL in clinical plasma samples. Furthermore, a comprehensive methodological validation of the detection method was conducted to ensure its accuracy, reliability, and reproducibility. The accuracy of the detection data for each batch was ensured by including a standard curve and QC data in each analytical batch. These features examined in this study establish a solid foundation for the further use and statistical analysis of this method.

Although prior studies have described the method by which glycosylation end products are created, there has been no large-scale investigation comparing the results of CML and CEL in thousands of plasma samples to show an association with aging. The aim of this study was to simultaneously examine several of the factors that influence aging. Based on the data on the effects of aging on glucose metabolism, statistical analysis of different age groups showed that the trends of changes in these two indicators with aging were basically similar; specifically, they both showed a significant increase between 40 and 80 years, with an average increase of 9.06 and 8.00% per decade of aging, respectively.

However, there was a slight difference between genders: the percentage of increase in CML was 10.89 and 7.39% for males and females, respectively, with a difference of 3.5%; the percentage of increase in CEL was 8.56 and 7.76% for males and females, respectively, with a difference of 0.8%. The difference in age-related changes between genders was more significant in CML values than that of CEL, suggesting that clinically, CML is a more sensitive indicator for males, while for females, both CML and CEL are equally qualified as evaluation indicators.

In terms of the overall trend, both indicators showed a correlation trend with age in both male and female groups, and this correlation was similar in male and female samples. Hence, these two indicators can be recommended as assistant indicator with relatively broad applicability for assessing the impact of aging on glucose metabolism.

While CML and CEL have shown some utility as clinical indicators in studies related to aging, more research is needed to further validate their effectiveness and understand the underlying mechanisms. Recent research findings have suggested that CML is also influenced by the levels of acetaldehyde in healthy individuals [ 22 ]. There are also studies that have reported the impact of dietary sugars and related endogenous advanced glycation end products on the extent of chromosomal DNA damage in WIL2-NS cells [ 21 ]. These findings clearly indicate that aging is a complex process influenced by multiple factors, and using a single indicator can have only limited value. Therefore, a combination of multiple biomarkers and clinical indicators needs to be considered for a more accurate assessment of the risk due to aging and health status.

In conclusion, the assay developed in this study fulfilled the clinical requirements for the rapid detection of CML and CEL in human plasma samples. Its sensitivity, precision, and accuracy were also qualified, and the results were accurate and reliable. The findings of this study provide information on the trends in changes of these two markers across different age groups with respect to the evaluation of glucose metabolism. However, further validation is required through additional research into the related mechanisms. The analytical detection method established in this study was accurate, reliable, and highly sensitive, providing a methodological and practical foundation for further research in the realm of human health monitoring.

Availability of data and materials

The datasets used and/or analysed during the current study available from the corresponding author on reasonable request. We declared that materials described in the manuscript, including all relevant raw data, will be freely available to any scientist wishing to use them for non-commercial purposes, without breaching participant confidentiality.

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Acknowledgments

We would like to acknowledge the hard and dedicated work of all the staff that implemented the intervention and evaluation components of the study.

This work was supported by Ministry of Health Industry Special Project (No.201302008).

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Daguang Wang

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Beijing Hospital Laboratory, National Center for Gerontology, No.1 of Dahua Road, Dongcheng District, Beijing, 100000, China

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Conception and design of the research: Daguang Wang. Acquisition of data: Junshan Wang, Xiaofeng Yang, Tianyi Liu, Qian Liu, Meng Wang. Analysis and interpretation of the data: Daguang Wang, Junshan Wang, Xinghong Liu, Kehe Du, Xiaofeng Yang, Tianyi Liu. Statistical analysis: Junshan Wang, Qian Liu, Meng Wang. Obtaining financing: Jian Guo. Writing of the manuscript: Daguang Wang, Junshan Wang. Critical revision of the manuscript for intellectual content: Daguang Wang, Junshan Wang, Xinghong Liu, Kehe Du,Hongjun Liu. All authors read and approved the final draft.

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Wang, D., Wang, J., Liu, X. et al. Quantifying carboxymethyl lysine and carboxyethyl lysine in human plasma: clinical insights into aging research using liquid chromatography-tandem mass spectrometry. BMC Biotechnol 24 , 12 (2024). https://doi.org/10.1186/s12896-024-00838-5

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Received : 03 November 2023

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DOI : https://doi.org/10.1186/s12896-024-00838-5

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Pesticide residues in fresh Mazafati date fruit, soil, and water, and assessment of potential health risks to consumers

• Research Article
• Published: 11 March 2024

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• Fatemeh Ganjeizadeh Rohani   ORCID: orcid.org/0000-0001-6403-9391 1 ,
• Vahideh Mahdavi 2 &
• Mohammad Javad Assari 1  

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A quantitative method based on quick, easy, cheap, effective, rugged, and safe technique (QuEChERS) sample extraction and ultra-performance liquid chromatography/tandem mass spectrometry (UHPLC-MS/MS) was evolved for the determination of 47 pesticide residues in fresh Mazafati date fruits from Bam City of Kerman Province, Iran. The recoveries for selected pesticides ranged from 88 to 110% with a relative standard deviation (RSD) of less than 20% at concentrations of 0.05 and 0.1 mg kg −1 . The proposed method had a linear range from the limit of quantification (LOQ) to 1.00 mg kg −1 , and the LOQ of the 47 pesticides was ≤ 0.005 mg kg −1 . The coefficients of determination ( R 2 ) were more than 0.99. This technique was used on 12 fresh date fruits samples, three water samples, and three soil samples with three replications per sample. Forty-seven pesticide were detected collectively, but only diazinon was detected in the date fruit samples. The mean value of diazinon residues was 0.037 mg kg −1 , and the concentration of diazinon in most samples was below the national maximum residue limit (MRL) for date fruit (0.05 mg kg −1 ). Among the pesticides measured, diazinon residues were also detected in the water samples, but not in the soil samples. The dietary intake assessment showed no health risk to humans from the consumption of fresh date fruit concerning the pesticides investigated.

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Acknowledgements

The authors are thankful to Kerman’s Agricultural and Natural Resources Research and Education Center that supported this project.

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Plant Protection Research Department, Kerman Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran

Fatemeh Ganjeizadeh Rohani & Mohammad Javad Assari

Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

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Contributions

All authors contributed to the study’s conception and design. Fatemeh Ganjeizadeh Rohani, Vahideh Mahdavi, and Mohammad Javad Assari performed material preparation, data collection, and analysis. The first draft of the manuscript was written by Fatemeh Ganjeizadeh Rohani, and all authors commented on previous versions. All authors read and approved the final manuscript.

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Correspondence to Fatemeh Ganjeizadeh Rohani .

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Rohani, .G., Mahdavi, V. & Assari, M.J. Pesticide residues in fresh Mazafati date fruit, soil, and water, and assessment of potential health risks to consumers. Environ Sci Pollut Res (2024). https://doi.org/10.1007/s11356-024-32760-w

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Received : 04 October 2023

Accepted : 29 February 2024

Published : 11 March 2024

DOI : https://doi.org/10.1007/s11356-024-32760-w

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