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Veterinary Medicine Research Paper Topics

Veterinary medicine research paper topics encompass a wide range of subjects that contribute to the advancement of animal healthcare. This page provides a comprehensive guide for students studying veterinary medicine who are tasked with writing research papers. Explore the intricacies of this field, delve into diverse categories, and discover a multitude of compelling topics to delve into. Whether you’re interested in animal behavior, infectious diseases, pharmacology, or veterinary surgery, this guide will help you navigate the realm of veterinary medicine research paper topics. By offering expert advice on topic selection and providing valuable insights on how to write an impactful research paper, we aim to empower students to make significant contributions to the field of veterinary medicine. Furthermore, iResearchNet’s writing services ensure that students receive top-quality, customized research papers tailored to their unique requirements. Let us help you unleash your academic potential and make a lasting impact in the world of veterinary medicine.

100 Veterinary Medicine Research Paper Topics

Introduction: The field of veterinary medicine encompasses a vast array of disciplines and areas of study, offering a wealth of research opportunities for students. This comprehensive list of veterinary medicine research paper topics is divided into 10 categories, each containing 10 unique topics. By exploring these topics, students can gain a deeper understanding of various aspects of veterinary medicine and contribute to the advancement of animal healthcare.

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Animal Behavior and Psychology:

• The impact of environmental enrichment on animal behavior and welfare
• Behavioral interventions for managing aggression in dogs
• Understanding the role of animal cognition in training and behavior modification
• The relationship between human-animal interaction and animal behavior
• Investigating stress and coping mechanisms in companion animals
• The effects of socialization on the behavior and development of puppies and kittens
• Exploring the psychological well-being of captive animals in zoos
• Behavioral indicators and management strategies for pain in animals
• Understanding the behavior and welfare of farm animals in intensive production systems
• Investigating the impact of fear and anxiety on animal welfare in veterinary settings

Infectious Diseases:

• Emerging zoonotic diseases and their impact on public health
• Antimicrobial resistance in veterinary medicine: challenges and strategies
• The role of vaccination in preventing infectious diseases in companion animals
• Epidemiology and control measures for common bacterial infections in livestock
• Investigating the transmission dynamics of vector-borne diseases in animals
• Diagnostic methods and advancements in the detection of viral infections in animals
• One Health approach: addressing the link between animal and human infectious diseases
• The impact of climate change on the prevalence and distribution of infectious diseases in wildlife
• Surveillance and control measures for emerging viral diseases in aquaculture
• Exploring the impact of biosecurity measures in preventing the spread of infectious diseases in veterinary clinics and hospitals

Pharmacology and Therapeutics:

• Investigating the efficacy and safety of new veterinary drugs and therapies
• Pharmacokinetics and pharmacodynamics of commonly used drugs in veterinary practice
• Adverse drug reactions and drug interactions in veterinary medicine
• Exploring alternative therapies in veterinary medicine: acupuncture, herbal medicine, and more
• The role of personalized medicine in veterinary practice
• Drug-resistant parasites and strategies for their control in companion animals
• Investigating the use of pain management protocols in veterinary surgery
• The impact of nutraceuticals and dietary supplements on animal health
• Pharmacogenomics in veterinary medicine: implications for personalized treatment
• Exploring the challenges and opportunities in veterinary drug development

Veterinary Surgery and Anesthesia:

• Advancements in minimally invasive surgery in veterinary medicine
• Anesthetic management and monitoring in exotic animal species
• Investigating surgical techniques for the treatment of orthopedic conditions in companion animals
• Complications and management of anesthesia in geriatric patients
• Exploring the role of regenerative medicine in veterinary surgery
• Surgical interventions for the management of oncological conditions in animals
• Investigating novel approaches for pain management in postoperative veterinary patients
• Surgical techniques and rehabilitation strategies for the treatment of spinal cord injuries in animals
• Exploring the use of robotic surgery in veterinary medicine
• Investigating the impact of surgical interventions on the quality of life in animals

Diagnostic Imaging and Radiology:

• Advancements in imaging techniques for the early detection of cancer in animals
• Investigating the use of magnetic resonance imaging (MRI) in veterinary neurology
• The role of ultrasound in diagnosing and managing cardiovascular diseases in animals
• Radiographic evaluation and interpretation of musculoskeletal disorders in small animals
• Investigating the use of computed tomography (CT) in veterinary oncology
• Diagnostic imaging in avian and exotic animal medicine
• The impact of advanced imaging modalities on the diagnosis of gastrointestinal diseases in animals
• Exploring the role of nuclear medicine in veterinary diagnostics
• Radiographic evaluation and interpretation of respiratory disorders in large animals
• Investigating the use of contrast-enhanced imaging techniques in veterinary medicine

Veterinary Public Health and Epidemiology:

• One Health approach in the surveillance and control of zoonotic diseases
• Investigating foodborne pathogens and their impact on animal and human health
• The role of veterinarians in disaster preparedness and response
• Veterinary epidemiology: studying disease patterns and risk factors in animal populations
• Investigating the impact of environmental factors on animal health and well-being
• Exploring the relationship between animal agriculture and antimicrobial resistance
• Veterinary public health interventions for the prevention of zoonotic diseases
• The role of wildlife in the transmission of infectious diseases to domestic animals
• Investigating the impact of climate change on vector-borne diseases in veterinary medicine
• Surveillance and control measures for emerging and re-emerging diseases in veterinary public health

Animal Nutrition and Feed Science:

• Investigating the impact of diet and nutrition on companion animal health
• The role of nutritional interventions in the management of obesity in animals
• Exploring the nutritional requirements and feed formulations for exotic animal species
• Nutritional strategies for the prevention and management of metabolic diseases in livestock
• Investigating the impact of feed additives on animal performance and health
• The role of probiotics and prebiotics in promoting gut health in animals
• Nutritional management of common gastrointestinal disorders in companion animals
• Exploring sustainable and environmentally friendly feed options for livestock
• Investigating the impact of nutrition on reproductive performance in animals
• Nutritional considerations for the optimal growth and development of neonatal animals

Veterinary Education and Professional Development:

• Evaluating the effectiveness of veterinary education programs in preparing students for practice
• Investigating the role of simulation-based training in veterinary education
• Exploring innovative teaching methods in veterinary schools
• Assessing the impact of continuing education on veterinary professionals’ knowledge and skills
• Investigating the factors influencing career choices among veterinary students
• The impact of telemedicine on veterinary practice and client communication
• Exploring the challenges and opportunities in veterinary entrepreneurship
• Veterinary leadership and management skills for effective practice management
• Investigating the role of mentorship in veterinary education and professional development
• Exploring the ethical considerations in veterinary practice and research

Equine Medicine and Surgery:

• Investigating advancements in diagnostic imaging techniques for equine lameness
• Management strategies for musculoskeletal disorders in performance horses
• The impact of nutrition and exercise on the prevention and management of metabolic diseases in horses
• Exploring the use of regenerative therapies in equine orthopedics
• Investigating the impact of respiratory diseases on the performance and welfare of horses
• Equine dentistry: advancements in dental care and oral health management
• Exploring novel surgical interventions for the treatment of orthopedic conditions in horses
• The role of physical therapy and rehabilitation in equine medicine
• Investigating the impact of exercise physiology on performance enhancement in horses
• Infectious diseases and vaccination strategies in equine healthcare

Wildlife Medicine and Conservation:

• Investigating the impact of habitat loss on wildlife health and conservation
• Wildlife forensic medicine: techniques for investigating wildlife crimes
• The role of veterinarians in wildlife rehabilitation and release programs
• Exploring the impact of emerging infectious diseases on wildlife populations
• Investigating the use of contraception in wildlife population management
• Wildlife anesthesia and immobilization techniques for veterinary interventions
• Exploring the role of veterinary medicine in endangered species conservation
• Investigating the impact of pollution and environmental contaminants on wildlife health
• Wildlife diseases and their potential for spillover to domestic animal populations
• Conservation genetics: utilizing molecular techniques in wildlife management

This comprehensive list of veterinary medicine research paper topics provides students with a wide range of subjects to explore within the field. Whether you are interested in animal behavior, infectious diseases, pharmacology, surgery, or any other aspect of veterinary medicine, there are countless opportunities for research and innovation. By selecting a topic that aligns with your interests and career goals, and following the expert advice on how to choose and write a research paper, you can contribute to the advancement of veterinary medicine and make a lasting impact on animal health and welfare.

Veterinary Medicine: Exploring the Range of Research Paper Topics

Veterinary medicine plays a vital role in the health and well-being of animals, from beloved pets to livestock and wildlife. As a student studying veterinary medicine, you have the opportunity to delve into various research areas and contribute to advancements in animal healthcare. This article will explore the diverse range of research paper topics available within the field of veterinary medicine, offering you insights into the exciting and impactful areas of study.

• Animal Nutrition and Feed Science : Proper nutrition is fundamental to the health and well-being of animals. Research topics in this area could include investigating the impact of diet and nutrition on companion animal health, exploring nutritional interventions for managing metabolic diseases in livestock, and examining sustainable and environmentally friendly feed options for animals.
• Infectious Diseases : Infectious diseases pose significant challenges to animal health and public health. Research paper topics in this category could encompass emerging zoonotic diseases and their impact on human health, antimicrobial resistance in veterinary medicine, vaccination strategies for preventing infectious diseases in animals, and exploring the transmission dynamics of vector-borne diseases.
• Animal Behavior and Psychology : Understanding animal behavior and psychology is essential for providing optimal care. Research topics in this field may involve studying the impact of environmental enrichment on animal behavior and welfare, behavioral interventions for managing aggression in dogs, investigating the cognitive abilities of animals, and exploring the role of human-animal interaction in animal behavior.
• Pharmacology and Therapeutics : Pharmacology plays a critical role in treating and preventing diseases in animals. Research paper topics in this area could include investigating the efficacy and safety of new veterinary drugs and therapies, exploring alternative therapies such as acupuncture and herbal medicine, and studying the pharmacokinetics and pharmacodynamics of commonly used drugs in veterinary practice.
• Veterinary Surgery and Anesthesia : Surgical interventions are often necessary for diagnosing and treating various conditions in animals. Research topics in this category could focus on advancements in minimally invasive surgery, investigating anesthesia management and monitoring in different animal species, exploring regenerative medicine in veterinary surgery, and studying the impact of surgical interventions on the quality of life in animals.
• Diagnostic Imaging and Radiology : Diagnostic imaging techniques play a crucial role in diagnosing and monitoring diseases in animals. Research paper topics in this field may include advancements in imaging techniques for detecting cancer in animals, exploring the use of magnetic resonance imaging (MRI) and computed tomography (CT) in veterinary diagnostics, and investigating the application of radiography and ultrasound in diagnosing specific conditions.
• Veterinary Public Health and Epidemiology : Veterinary medicine intersects with public health in various ways. Research topics in this area could involve the One Health approach in the surveillance and control of zoonotic diseases, studying the impact of environmental factors on animal and human health, and investigating the link between animal agriculture and antimicrobial resistance.
• Equine Medicine and Surgery : Horses require specialized veterinary care due to their unique physiology and performance demands. Research paper topics in this category may include investigating advancements in diagnostic imaging techniques for equine lameness, studying the management strategies for musculoskeletal disorders in performance horses, and exploring the impact of respiratory diseases on horse performance and welfare.
• Wildlife Medicine and Conservation : The health and conservation of wildlife are essential for maintaining biodiversity. Research topics in this field could include studying the impact of habitat loss on wildlife health, investigating wildlife rehabilitation and release programs, exploring the role of veterinarians in wildlife conservation, and understanding the diseases that affect wildlife populations.
• Veterinary Education and Professional Development : Ensuring the competency and continuous development of veterinary professionals is crucial. Research paper topics in this area may involve evaluating veterinary education programs, exploring innovative teaching methods, studying the impact of continuing education on veterinary professionals’ knowledge and skills, and investigating the factors influencing career choices among veterinary students.

The field of veterinary medicine offers a wide range of research opportunities, spanning various disciplines and species. Whether you are interested in animal nutrition, infectious diseases, surgery, diagnostic imaging, public health, or any other aspect of veterinary medicine, there are numerous fascinating topics to explore. By selecting a research paper topic that aligns with your interests and goals, you can contribute to the advancement of veterinary medicine, improve animal health and welfare, and make a meaningful impact in the field.

Choosing Veterinary Medicine Research Paper Topics

Selecting the right research paper topic is crucial for your success as a student of veterinary medicine. It allows you to delve into an area of interest, contribute to existing knowledge, and explore the latest advancements in the field. In this section, we will provide you with expert advice on how to choose veterinary medicine research paper topics that align with your interests and academic goals.

• Identify Your Interests : Start by reflecting on your personal interests within the field of veterinary medicine. Consider the areas that fascinate you the most, such as animal behavior, infectious diseases, surgery, diagnostic imaging, wildlife medicine, or public health. Identifying your passions will make the research process more enjoyable and rewarding.
• Consult Your Professors and Mentors : Seek guidance from your professors and mentors who have expertise in different veterinary medicine disciplines. They can provide valuable insights into current research trends, emerging topics, and areas that need further exploration. Discuss your interests with them, and they can help you narrow down potential research paper topics based on their knowledge and experience.
• Stay Updated with Current Literature : Stay abreast of the latest research publications, scientific journals, and conference proceedings in the field of veterinary medicine. Regularly reading scientific literature will expose you to new research findings, innovative techniques, and emerging topics. This will help you identify gaps in the existing knowledge that you can address through your research paper.
• Consider Relevance and Impact : When selecting a research topic, consider its relevance and potential impact on veterinary medicine. Look for topics that address current challenges, emerging issues, or areas where advancements are needed. Research that can contribute to animal health, welfare, conservation, or public health will not only be academically fulfilling but also have real-world implications.
• Analyze Feasibility : Assess the feasibility of your chosen research topic in terms of available resources, time constraints, and access to data. Consider the availability of research materials, laboratory facilities, animal populations, or specialized equipment required for your study. Ensure that your chosen topic is practical and achievable within the given timeframe and available resources.
• Collaborate with Peers : Consider collaborating with your peers or fellow researchers who share similar research interests. Collaborative research projects can broaden your perspective, enhance the quality of your research, and facilitate knowledge sharing. Engaging in interdisciplinary collaborations can also help you explore topics that combine veterinary medicine with other fields, such as biology, ecology, or public health.
• Seek Inspiration from Case Studies and Clinical Experience : Drawing inspiration from case studies, clinical experiences, or real-world scenarios can lead to intriguing research topics. Reflect on challenging cases you have encountered during clinical rotations, unique observations, or clinical questions that have piqued your interest. These experiences can spark ideas for research that address practical veterinary medicine issues.
• Consider Ethical Considerations : When choosing a research topic, consider ethical considerations related to animal welfare and human subjects. Ensure that your research adheres to ethical guidelines and regulations. If your research involves animal subjects, be mindful of the ethical treatment and use of animals, and obtain necessary approvals from relevant ethics committees.
• Explore Emerging Technologies and Techniques : Advancements in technology and techniques have a significant impact on veterinary medicine. Consider topics that explore the application of emerging technologies such as genomics, telemedicine, artificial intelligence, or novel diagnostic tools in veterinary practice. Research in these areas can contribute to the evolution of veterinary medicine and improve animal healthcare outcomes.
• Seek Practical Relevance and Application : Choose research topics that have practical relevance and application in the veterinary field. Look for topics that address challenges faced by veterinarians, animal owners, or the industry. Research that can provide evidence-based solutions, improve clinical practices, or enhance disease prevention and management will have a direct impact on veterinary medicine.

Selecting a suitable research paper topic is a crucial step in your journey as a veterinary medicine student. By identifying your interests, seeking guidance, staying updated with current literature, considering relevance and impact, and analyzing feasibility, you can choose a research topic that is both intellectually stimulating and practically valuable. Remember to consider ethical considerations, collaborate with peers, and explore emerging technologies. By following these expert tips, you will be well-equipped to embark on a research project that contributes to the advancement of veterinary medicine and makes a positive impact on animal health and welfare.

How to Write a Veterinary Medicine Research Paper

Writing a research paper in veterinary medicine allows you to contribute to the field, explore new knowledge, and develop critical thinking and scientific communication skills. In this section, we will guide you through the process of writing a veterinary medicine research paper, from selecting a topic to crafting a compelling paper that effectively communicates your findings.

• Define Your Research Objectives : Clearly define the objectives of your research paper. Determine what you aim to accomplish and the specific research questions you want to answer. This will provide a clear focus and direction for your study.
• Conduct a Thorough Literature Review : Begin by conducting a comprehensive literature review to gather existing knowledge and identify gaps in the research. Analyze and critically evaluate relevant studies, articles, and scientific literature to establish the context for your research.
• Refine Your Research Question : Based on your literature review, refine your research question or hypothesis. Ensure that your question is specific, measurable, achievable, relevant, and time-bound (SMART). This will guide your research and help you stay focused.
• Design Your Study : Select an appropriate research design and methodology that aligns with your research question and objectives. Determine the sample size, data collection methods, and statistical analyses required. Ensure that your study design is rigorous and ethically sound.
• Gather and Analyze Data : Collect relevant data using appropriate research methods, whether it involves conducting experiments, surveys, interviews, or analyzing existing datasets. Ensure that your data collection is thorough, reliable, and accurately recorded. Use appropriate statistical tools to analyze your data and draw meaningful conclusions.
• Organize Your Paper : Structure your research paper in a logical and organized manner. Include sections such as the introduction, literature review, methods, results, discussion, and conclusion. Follow a clear and coherent flow of information that guides the reader through your research process.
• Write an Engaging Introduction : Start your paper with an engaging introduction that provides background information on the topic, states the research problem, and highlights the significance of your study. Clearly articulate your research objectives and hypotheses to set the stage for the rest of the paper.
• Present a Comprehensive Literature Review : Incorporate a thorough literature review in the body of your paper. Summarize and critically analyze relevant studies, theories, and findings that inform your research. Identify gaps in the literature and highlight the unique contribution of your study.
• Describe Your Methods and Results : Clearly explain the methods you employed to conduct your research and gather data. Provide sufficient detail for others to replicate your study. Present your results objectively, using appropriate tables, graphs, or figures to support your findings. Interpret the results and discuss their implications.
• Engage in a Thoughtful Discussion : In the discussion section, interpret your findings in the context of existing knowledge and theories. Discuss the implications of your results, their limitations, and any future directions for research. Address any unanswered questions and propose areas for further investigation.
• Write a Strong Conclusion : Summarize your main findings and their significance in a concise and impactful conclusion. Restate your research objectives and hypotheses, and emphasize how your study contributes to the field of veterinary medicine. Avoid introducing new information in the conclusion.
• Cite Sources Accurately : Ensure that you cite all the sources used in your research paper accurately. Follow the appropriate citation style, such as APA, MLA, or Chicago, and adhere to the specific guidelines for referencing scientific literature and other relevant sources.
• Revise and Proofread : After completing the initial draft, revise your paper for clarity, coherence, and logical flow. Check for grammatical and spelling errors, and ensure that your writing is concise and precise. Seek feedback from peers, mentors, or professors to improve the quality of your paper.

Writing a veterinary medicine research paper requires careful planning, attention to detail, and effective communication skills. By defining your research objectives, conducting a thorough literature review, designing a rigorous study, and organizing your paper coherently, you can produce a high-quality research paper. Remember to write an engaging introduction, present a comprehensive literature review, describe your methods and results accurately, engage in thoughtful discussion, and provide a strong conclusion. Cite your sources properly and revise your paper meticulously. Through this process, you will contribute to the field of veterinary medicine and advance knowledge in the domain.

iResearchNet’s Writing Services

At iResearchNet, we understand the challenges that students face when it comes to writing research papers in veterinary medicine. We are here to provide you with professional writing services that cater to your specific needs. Our team of expert writers and researchers are well-versed in the field of veterinary medicine and can assist you in producing high-quality research papers. In this section, we will outline the range of services we offer and the benefits of choosing iResearchNet for your veterinary medicine research paper needs.

• Expert Degree-Holding Writers : Our team consists of expert writers with advanced degrees in veterinary medicine and related fields. They have a deep understanding of the subject matter and can deliver well-researched and meticulously written research papers.
• Custom Written Works : We provide custom written works that are tailored to your specific requirements. Whether you need a research paper from scratch or assistance with specific sections, our writers can create unique and original content that meets your academic standards.
• In-Depth Research : Our writers conduct extensive research to gather the most relevant and up-to-date information for your research paper. They have access to reputable sources and scientific databases to ensure the accuracy and validity of the information presented in your paper.
• Custom Formatting : We understand the importance of adhering to specific formatting styles required by academic institutions. Our writers are well-versed in various citation styles, including APA, MLA, Chicago/Turabian, and Harvard. They will format your paper according to the specific guidelines provided.
• Top Quality : Quality is our utmost priority. We strive to deliver research papers that meet the highest standards of academic excellence. Our writers pay attention to every detail, ensuring that your paper is well-structured, coherent, and free from grammatical errors.
• Customized Solutions : We recognize that each research paper is unique. Our writers work closely with you to understand your specific research objectives, requirements, and preferences. They can customize their approach to meet your specific needs and deliver a paper that aligns with your expectations.
• Flexible Pricing : We offer flexible pricing options to accommodate the budgetary constraints of students. Our pricing is competitive and transparent, ensuring that you receive the best value for your investment. We offer affordable rates without compromising on the quality of our services.
• Short Deadlines : We understand that students often face tight deadlines. Our team is equipped to handle urgent requests and can deliver high-quality research papers within short timeframes, even as tight as 3 hours. You can rely on us to meet your deadlines without compromising on quality.
• Timely Delivery : We prioritize timely delivery to ensure that you have sufficient time to review and submit your research paper. Our writers work diligently to complete your paper within the agreed-upon timeframe, allowing you ample time for any revisions or modifications you may require.
• 24/7 Support : We provide round-the-clock support to address any queries or concerns you may have. Our customer support team is available 24/7 to assist you with any questions regarding our services, order placement, or ongoing projects. Feel free to reach out to us at any time.
• Absolute Privacy : We understand the importance of confidentiality. Your personal information and the details of your research paper are treated with the utmost privacy and confidentiality. We adhere to strict data protection protocols to ensure the security of your information.
• Easy Order Tracking : Our user-friendly platform allows you to easily track the progress of your research paper. You can communicate with your assigned writer, provide additional instructions or clarifications, and monitor the status of your order throughout the writing process.
• Money Back Guarantee : We are committed to customer satisfaction. In the unlikely event that you are not satisfied with the quality of the research paper or the services provided, we offer a money-back guarantee. Your investment is protected, and we strive to ensure your complete satisfaction.

Choosing iResearchNet for your veterinary medicine research paper needs ensures that you receive professional, reliable, and customized writing services. Our team of expert writers, in-depth research capabilities, adherence to formatting guidelines, and commitment to quality will ensure that your research paper meets the highest academic standards. With flexible pricing options, timely delivery, 24/7 support, absolute privacy, and easy order tracking, we strive to make your experience with iResearchNet seamless and rewarding. Place your trust in us and let our expertise guide you towards academic success.

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Are you struggling with your veterinary medicine research papers? Do you find it challenging to choose the right topics, conduct in-depth research, and meet the high academic standards of your institution? Look no further! iResearchNet is here to provide you with the professional support you need to excel in your veterinary medicine studies. Our team of expert writers and researchers is ready to assist you in crafting top-quality research papers that will impress your professors and elevate your academic performance.

By choosing iResearchNet, you gain access to a range of benefits that will make your research paper writing experience smooth, efficient, and stress-free. Our team consists of highly qualified writers with expertise in veterinary medicine and related fields. They are equipped with the necessary knowledge and skills to tackle even the most complex research topics. Whether you need assistance in selecting research paper topics, conducting thorough research, or structuring your paper, our experts are here to guide you every step of the way.

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Genomic and phenotypic analysis of invasive Streptococcus suis isolated in Spain reveals genetic diversification and associated virulence traits

Streptococcus suis is a zoonotic pathogen that causes a major health problem in the pig production industry worldwide. Spain is one of the largest pig producers in the world. This work aimed to investigate the ge...

An apicoplast-localized deubiquitinase contributes to the cell growth and apicoplast homeostasis of Toxoplasma gondii

Toxoplasma gondii is among the most important parasites worldwide. The apicoplast is a unique organelle shared by all Apicomplexan protozoa. Increasing lines of evidence suggest that the apicoplast possesses its ...

ZNF283, a Krüppel-associated box zinc finger protein, inhibits RNA synthesis of porcine reproductive and respiratory syndrome virus by interacting with Nsp9 and Nsp10

Porcine reproductive and respiratory syndrome virus (PRRSV) is a viral pathogen with substantial economic implications for the global swine industry. The existing vaccination strategies and antiviral drugs off...

Inflammatory responses and barrier disruption in the trachea of chicks following Mycoplasma gallisepticum infection: a focus on the TNF-α-NF-κB/MLCK pathway

Mycoplasma gallisepticum (MG) can induce persistent inflammatory damage to the tracheal mucosa of poultry and cause chronic respiratory diseases in chickens. To further investigate the mechanism of MG-induced inj...

Flubendazole carbonyl reduction in drug-susceptible and drug-resistant strains of the parasitic nematode Haemonchus contortus : changes during the life cycle and possible inhibition

Carbonyl-reducing enzymes (CREs) catalyse the reduction of carbonyl groups in many eobiotic and xenobiotic compounds in all organisms, including helminths. Previous studies have shown the important roles of CR...

Phenotypic and genotypic assessment of iron acquisition in diverse bovine-associated non- aureus staphylococcal strains

Although the role of iron in bacterial infections has been well described for Staphylococcus ( S .) aureus , iron acquisition in (bovine-associated) non- aureus staphylococci and mammaliicocci (NASM) remains insuffic...

The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens

Avian influenza viruses (AIV) of the H7N7 subtype are enzootic in the wild bird reservoir in Europe, cause infections in poultry, and have sporadically infected humans. The non-structural protein PB1-F2 is enc...

Quantitative label-free proteomic analysis of excretory-secretory proteins in different developmental stages of Trichinella spiralis

Trichinella spiralis ( T. spiralis ) is a zoonotic parasitic nematode with a unique life cycle, as all developmental stages are contained within a single host. Excretory-secretory (ES) proteins are the main targets...

Galectin from Trichinella spiralis alleviates DSS-induced colitis in mice by regulating the intestinal microbiota

According to numerous reports, Trichinella spiralis ( T. spiralis ) and its antigens can reduce intestinal inflammation by modulating regulatory immunological responses in the host to maintain immune homeostasis. G...

Characteristics of the a sequence of the duck Plague virus genome and specific cleavage of the viral genome based on the a sequence

During the replication process, the herpesvirus genome forms the head-to-tail linked concatemeric genome, which is then cleaved and packaged into the capsid. The cleavage and packing process is carried out by ...

Non-coding RNA in the gut of the blood-feeding parasitic worm, Haemonchus contortus

The intestine of Haemonchus contortus is an essential tissue that has been indicated to be a major target for the prevention of haemonchosis caused by this parasitic nematode of small ruminants. Biological peculi...

Vidofludimus inhibits porcine reproductive and respiratory syndrome virus infection by targeting dihydroorotate dehydrogenase

Porcine reproductive and respiratory syndrome virus (PRRSV) infection has caused huge economic losses in global swine industry over the last 37 years. PRRSV commercial vaccines are not effective against all ep...

Mitochondrial dysfunction induced by bedaquiline as an anti- Toxoplasma alternative

Toxoplasma gondii is a zoonotic parasite that infects one-third of the world’s population and nearly all warm-blooded animals. Due to the complexity of T. gondii’s life cycle, available treatment options have lim...

Early detection of infectious bovine keratoconjunctivitis with artificial intelligence

Artificial intelligence (AI) was developed to distinguish cattle by their muzzle patterns and identify early cases of disease, including infectious bovine keratoconjunctivitis (IBK). It was tested on 870 cattl...

Differential infection behavior of African swine fever virus (ASFV) genotype I and II in the upper respiratory tract

African swine fever virus (ASFV) is a substantial threat to pig populations worldwide, contributing to economic disruption and food security challenges. Its spread is attributed to the oronasal transmission ro...

The secretome of Staphylococcus aureus strains with opposite within-herd epidemiological behavior affects bovine mononuclear cell response

Staphylococcus aureus modulates the host immune response directly by interacting with the immune cells or indirectly by secreting molecules (secretome). Relevant differences in virulence mechanisms have been repo...

Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens

Clinical avian coccidiosis is typically caused by coinfection with several Eimeria species. Recombinant protein and DNA vaccines have shown promise in controlling coccidiosis. On this basis, DNA vaccines that enc...

Streptococcus ruminantium -associated sheep mastitis outbreak detected in Italy is distinct from bovine isolates

Streptococcus ruminantium is the causative agent of several bovine and ovine diseases, however reports are uncommon and application of whole genome sequencing to identify is rare. We report for the first time, a ...

Molecular characteristics and pathogenicity of a novel chicken astrovirus variant

It is well-established that the genetic diversity, regional prevalence, and broad host range of astroviruses significantly impact the poultry industry. In July 2022, a small-scale commercial broiler farm in Ch...

Molecular and functional characterization of Schistosoma japonicum annexin A13

Schistosomiasis is a neglected tropical disease that affects humans and animals in tropical and subtropical regions worldwide. Schistosome eggs are responsible for the pathogenesis and transmission of schistos...

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Exploring Veterinary Career Options

The following list is not exhaustive but provides an overview of careers where graduates of veterinary medical schools can effectively apply their Doctor of Veterinary Medicine (DVM) degrees.

• Private practice , either general practice or (with advanced training and experience) a specialty field, such as ophthalmology, orthopedics, aquatic animal medicine, marine biology, wildlife animal medicine, or emergency animal medicine.
• Corporate veterinary medicine , for example, with corporations that provide veterinary care, test human drugs for safety, or produce animal-related products.
• The Federal Government  employs veterinarians through the United States Department of Agriculture (USDA), National Institutes of Health (NIH), Centers for Disease Control (CDC), and Food and Drug Administration (FDA) working on biosecurity, environmental quality, public health, meat inspection, regulatory medicine, and agricultural animal health, or the investigation of disease outbreaks.
• The U.S. Army Corps  and  U.S. Air Force  offer career opportunities in areas such food safety and military working dog veterinary medicine. The military also provides advanced training in specialty areas for those who commit to service.
• Research , either in a university setting or with companies that produce animal-related products or pharmaceuticals.
• Teaching, either in academia or non-professionals schools.  With 40 percent of aging faculty in academia eligible for retirement over the next 10 years, projections indicate an increasing need for qualified academics to teach in all disciplines of veterinary medicine.
• Public Health , particularly with governmental agencies such as the United State Public Health Service, which works to control the transmission of animal-to-human (zoonotic) diseases.
• Food supply medicine,  with either the government or a food animal company.
• Global Veterinary Medicine , in private practice or with international agencies working in areas such as food production and safety or emerging diseases.
• Public Policy , working for governments on animal and zoonotic diseases, animal welfare, public health issues, or as consultants with non-governmental agencies.
• Shelter medicine , working with communities,and private or public agencies to ensure the health and well being of animal populations housed in shelters.

There are additional opportunities available with state and municipal governments, nonprofits, and in areas that require a background in comparative medicine.

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• A Career in Veterinary Medicine Overview

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American Journal of Veterinary Research

The mission of AJVR is to disseminate throughout the world the highest-impact, rigorously reviewed veterinary science research across the spectrum of species, using an online-only, open access format for maximum accessibility. The AJVR converted to a fully open access platform 6/1/22. Open access articles are licensed under the Creative Commons CC BY-NC license. CC BY licensing is available upon request. Archival material is freely available. All rights are reserved in archival material.

Online ISSN: 1943-5681

Volume 85 (2024): Issue 3 (Mar 2024)

K-wire is more damaging than standard or acrylic drill bits when evaluating torsional properties of rabbit ( oryctolagus cuniculi ) femurs, myxomatous mitral valve disease and associated pulmonary hypertension might increase serum angiopoietin-2 in dogs, prevalence of elbow dysplasia in 13 dog breeds in france: a retrospective radiographic study (2002–2022), two lateral flow assays for detection of anti-coccidioidal antibodies show similar performance to immunodiffusion in dogs with coccidioidomycosis, doppler ultrasound is more accurate than pulse oximeter plethysmography in the measurement of systolic arterial pressure from the median caudal artery in anesthetized dogs, serial injections of 4% polyacrylamide hydrogel have no detrimental effects in equine joints following clinical, histologic, and synovial biomarker evaluation, clippers are superior to scissors in the collection of hair for chemical analysis in companion dogs: a dog aging project preliminary study, equine gamma herpesvirus presence and viral load are not associated with equine glandular gastric disease, hemadsorption extracorporeal therapy removes cytokines ex vivo in horses, a newly developed recovery-enhancing device improves recovery scores during emergence from general anesthesia in horses: a randomized trial, vertebral heart size and vertebral left atrial size reference ranges in healthy miniature schnauzers, injection rate, scanning position, and values of parameters on pulmonary computed tomography perfusion in normal beagles, no vaccine interference between bovine coronavirus and bovine herpesvirus-1 in a randomized trial when coadministrating two intranasal modified-live viral vaccines to neonatal calves, a complex cyclical one health pathway drives the emergence and dissemination of antimicrobial resistance, applying science to animal welfare, bleb characteristics assessed by ultrasound biomicroscopy correlate with intraocular pressure after ahmed glaucoma valve surgery in dogs, ciprofloxacin enhances therapeutic levels of voriconazole through cyp450 inhibition in the common raven ( corvus corax ), possibly improving efficacy against aspergillosis: a pilot study, intranasal booster vaccination of beef steers reduces clinical signs following experimental coinfection with brsv and bhv-1 without reducing shedding of brd-associated bacteria, oral bacteria may affect conjunctival microorganisms in brachycephalic dogs: a preliminary study, pulse-wave analysis during routine blood pressure measurement shows potential to distinguish between sinus and nonsinus rhythm, splenic gene expression of cytokines at multiple time points following lipopolysaccharide challenge in layers, subcutaneous administration of ceftazidime at 20 and 40 mg/kg produces theoretically therapeutic plasma concentrations for at least 120 hours in red-eared sliders ( trachemys scripta elegans ), inhibitor of p-glycoprotein–mediated drug transport by curcumin in broiler chickens, modeling daily veterinary anesthetist patient care hours and probabilities of exceeding critical thresholds, unique resources to support visionary researchers, vesicular stomatitis virus in two species of rhinoceros at a california zoological park, cardiovascular disease in central bearded dragons ( pogona vitticeps ): 54 cases (2007–2022), evaluation of a novel, sensitive thyroid-stimulating hormone assay as a diagnostic test for thyroid disease in cats, neurofilament light chain concentration does not correlate with disease status in labrador retrievers affected with idiopathic laryngeal paralysis, hypothermia, anemia, hyperglycemia, and severe hypoglycemia are significant prognostic indicators of death in client-owned ferrets ( mustela putorius furo ), an 18-µm microaggregate blood filter does not cause hemolysis during in vitro whole blood transfusions in sea turtles, how does evolutionary evaluation illuminate body size among canids, implementation of a prototype dynamic laryngoplasty system in standing sedated horses provided arytenoid abduction control at seven days postoperatively, single-dose nonsteroidal anti-inflammatory drugs in horses have no impact on concentrations of cytokines or growth factors in autologous protein solution and platelet-rich plasma, the edwards acumen iq system using peripheral arterial catheter-based waveforms to estimate cardiac output is not accurate as compared to thermodilution in dogs, evaluation of viscoelastic coagulation monitoring parameters and fibrinogen concentrations in healthy dogs undergoing stifle arthroscopy and tibial plateau leveling osteotomy, low levels of microrna-21 in neutrophil-derived exosomes may contribute to airway smooth muscle hyperproliferation in horses with severe asthma, pulmonary hypertension is associated with hypocoagulability in dogs: a retrospective analysis of 66 cases (2013–2021), reduced ribonucleotide reductase rrm2 subunit expression increases dna damage and mitochondria dysfunction in woody breast chickens, seroprevalence of reproductive and infectious diseases in cattle: the case of madre de dios in the peruvian southeastern tropics, what do veterinary professionals and clients want using discrete choice methods in veterinary research to determine preferences, arterial blood gas measurements are different for brachycephalic and nonbrachycephalic dogs acclimatized to an altitude of 1,535 meters, effects of diet on the bacterial and eukaryotic microbiota across the gastrointestinal tract of healthy rabbits ( oryctolagus cuniculus ), three-minute nebulization of gentamicin in healthy dogs results in therapeutic concentrations in bronchoalveolar lavage fluid while remaining below the toxic range values in blood, the fecal bacterial microbiota is not useful for discriminating between lymphoplasmacytic enteritis and low-grade intestinal t-cell lymphoma in cats nor for predicting therapeutic response, candidate urinary biomarkers show promise for distinguishing between calcium oxalate versus struvite urolithiasis in dogs, a single injection of high-concentration buprenorphine significantly reduces food and water intake as well as fecal and urine production in new zealand white rabbits ( oryctolagus cuniculus ), efficacy and safety of subcutaneous methadone hydrochloride in four-toed hedgehogs ( atelerix albiventris ), isoflurane anesthesia alters complete blood count values in lilac-crowned parrots ( amazona finschi ), quality assessment and characterization of unregulated antiviral drugs for feline infectious peritonitis: implications for treatment, safety, and efficacy, current state and future directions for veterinary antimicrobial resistance research, rectally obtained fecal samples can be used for fecal occult blood testing in dogs, and fecal immunochemical tests do not detect canine or feline blood, retrospective study of the early clinical experience with a precontoured angle-stable interlocking nail for fracture repair in dogs and cats, antibacterial activity of silver nanoparticles against escherichia coli and methicillin-resistant staphylococcus pseudintermedius is affected by incorporation into carriers for sustained release, serum activities of complement 1q and antibodies to the virulence-associated protein a are lower in foals that develop rhodococcal pneumonia, echocardiographic values and prevalence of cardiac abnormalities in clinically healthy adult borzoi dogs, next-generation sequencing-based liquid biopsy may be used for detection of residual disease and cancer recurrence monitoring in dogs, exposure to emulsified isoflurane and sevoflurane protects canine primary hepatocytes against hypoxia-induced apoptosis, predicting an increased risk of severe clinical mastitis and economic loss using a threshold value of bovine leukemia virus proviral load, commercially available wearable health monitor in dogs is unreliable for tracking energy intake and expenditure, exercising sheep as a preclinical model for musculoskeletal research, optimization of lung ventilation and perfusion in anesthetized horses using a ventilation mode with flow-limited expiration, aims and scope .

AJVR is a monthly, peer-reviewed, veterinary medical journal owned by the American Veterinary Medical Association that publishes reports of original research and review articles in the general area of veterinary medical research.

The mission of the AJVR is to publish, in a timely manner, peer-reviewed reports of the highest-quality research that has the clear potential to enhance the health, welfare, and performance of animals and humans. The journal will maintain the highest ethical standards of scientific journalism and promote such standards among its contributors. In addition, the journal will foster global interdisciplinary cooperation in veterinary medical research.

AJVR supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, clinical pathology, epidemiology, genetics, infectious disease, microbiology, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animal species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary or pilot studies are not appropriate.

Business Model

Open access

AJVR converted to a fully open access journal in June 2022 to better serve the interests of researchers and funders. Open access means that all content is freely available without charge to the user or the user’s institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other purpose permitted under the applicable License, without asking for prior permission from the publisher or the author. Under this model, authors or their research funders or institutions pay an article processing charge (APC) in order to make the article immediately free to access, download, and share anywhere in the world. Archival material (ie, pre-June 2022) is freely available. All rights are reserved in archival material.

Publication fees

The APC for AJVR is $1,250 for newly submitted articles effective January 1, 2024.

APC waivers may be granted at the Editor-in-Chief’s discretion.

License to publish

Articles published starting with the June 2022 issue are licensed for publication under the CC BY-NC Creative Commons License. Authors retain copyright in their work, which others may copy, redistribute, and transform, provided users give appropriate attribution to the original author(s) and do not use the content for commercial purposes. CC BY licensing is available upon request.

An article prepared by a U.S. federal government employee as part of the employee's official duties, or which is an official U.S. government publication, is called a "U.S. Government work" and is in the public domain in the United States. This does not mean that the article is automatically open access; rather, it means there is no copyright to transfer under U.S. law. AJVR makes such articles freely available at publication.

Editorial Policies

Peer review

AJVR uses a single anonymized review process. Reviewer identities are not, and should not be, disclosed to the authors or other reviewers. In addition, authors should not contact those whom they presume to be reviewers of their manuscripts. Each submitted manuscript is initially assessed by the Editor-in-Chief for suitability for the journal and may be assigned to an Associate Editor to initiate peer review by a minimum of 2 independent experts. When reviews are received, the Associate Editor recommends further revisions or rejection without further review, and the Editor-in-Chief, who may include additional comments, sends the decision to the authors.

Individuals should be listed as authors only if they:

• Made a substantial contribution to the conception or design of the study, acquisition of the data used in the study, or analysis and interpretation of that data, AND
• Were involved in drafting or revising the manuscript critically for important intellectual content, AND
• Will have the opportunity to approve the submitted and all subsequent versions, AND
• Agree to be accountable for the validity of the data and results.

Individuals who contributed to the study but do not meet all 4 of these criteria should not be listed as authors but should be included in the Acknowledgments section. Requests to list a working group or study group in the byline will be handled on a case-by-case basis.

• Authors are responsible for ensuring that their manuscripts are written clearly in English and formatted in accordance with these author instructions.
• Manuscripts should be carefully reviewed prior to submission for format, clarity, spelling, and grammar.
• A manuscript deemed by the editorial team to require extensive revisions prior to further consideration will be rejected with the option to resubmit after reformatting and/or editing has been performed.
• The journal does not endorse any specific editing service and provides these examples of suitable services that authors might consider: Cactus ,  Bioscience Writers , American Journal Experts , and the Nature Research Editing Service . Please note that when working with a professional language-editing service, authors should choose full medical editing with journal formatting, rather than simply proofreading, and that changes should comply with these instructions for authors.

Acknowledgments, disclosures, and funding

Authors will be required to address these 3 statements during submission. This information does not need to be included in the manuscript document.

• Acknowledgments: Identify individuals who made important contributions to the study but who do not meet the  criteria for authorship . If none , include the statement “None reported.”
• Include any conflicts of interest related to the manuscript. Include relevant financial interests (eg, ownership, employment, consultancies, honoraria, paid expert testimony, grants, patent-licensing arrangements, equity interests, and service as an officer or board member), activities, relationships, and affiliations (other than those listed on the title page of the manuscript). If none , include the statement “The authors have nothing to disclose.”
• Include the use of any artificial intelligence (AI)–assisted technology such as ChatGPT or another large language model in the writing of the manuscript or production of images. If none were used, include the statement “No AI-assisted technologies were used in the generation of this manuscript.” If an AI tool was used, the authors must be transparent in disclosing here, in the disclosures section, which AI tool was used and how the AI tool was used. AI tools cannot be listed as an author of a manuscript.
• Funding: Include all funding, other financial support, and material support obtained directly or indirectly from any third party in connection with information included in the manuscript or with the writing or publishing of the manuscript. If none , include the statement “The authors have nothing to disclose.”

Copyright permissions

• Permissions for use of figures, tables, or other content owned by another party: If your manuscript contains previously published material (text, images, tables, photographs, drawings, etc), you are responsible for providing proof of permission for reuse from the original copyright holder. At the time of submission of your manuscript to our journal, please include (1) permission for reuse of figures or tables and (2) a full citation for the previous publication. We cannot publish any previously published illustration or table without written permission from the original copyright holder.

Commercial availability, compounding, and extralabel drug use

• If a manuscript reports results of a study that involved evaluating a pharmaceutical, biologic, or other product that is not legally available in the US for use in that species, then a clear disclaimer is needed in the Methods section describing where the drug is commercially available and that the drug is neither FDA approved nor commercially available in the US.
• If compounded products/drugs were used in a study, an explanation in the Discussion section should include why the authors chose to use compounded products and what key factors should be considered by veterinarians interested in prescribing similarly prepared products.
• The Methods section should contain a complete description of the compounded products.
• Extralabel drug use must comply with the provisions of the Animal Medicinal Drug Use Clarification Act .
• Extralabel drug use was performed with owner consent and complied with provisions of AMDUCA and 21 CFR §530.
• Compounded products were prepared from bulk substances because [provide reason]. Veterinarians should adhere to compounding regulations and be aware that pharmacokinetic properties may differ between compounded and FDA-approved products.
• Compounded products were prepared from FDA-approved products because [provide reason]. Veterinarians should adhere to compounding regulations and be aware that pharmacokinetic properties may differ between compounded and FDA-approved products.

Humane animal care and use

• Research studies involving animals must have been performed in compliance with guidelines outlined in the US Animal Welfare Act , US Public Health Service Policy on the Humane Care and Use of Laboratory Animals , National Research Council’s Guide for the Care and Use of Laboratory Animals , or Guide for the Care and Use of Agricultural Animals in Research and Teaching or in compliance with equivalent guidelines. If animals were euthanized, the method of euthanasia must be indicated in the manuscript. Methods of euthanasia must comply with the AVMA Guidelines for the Euthanasia of Animals of jav.ma/GEA . If a method not recommended by the AVMA Guidelines on Euthanasia was used, a justification for use of this method must be provided.

Institutional oversight and owner consent

• With the exception of reports of retrospective studies based solely on historical data, manuscripts describing studies that involved the use of animals, including studies that involved the use of privately owned animals (eg, animals owned by clients, staff members, students, or private entities), must include a statement that the study protocol was reviewed and approved by an appropriate oversight entity (eg, an animal care and use committee or institutional review board) or was performed in compliance with institutional or other (eg, governmental or international) guidelines for research on animals.
• Prospective studies that involved privately owned animals must also include a statement indicating that informed owner consent was obtained.
• Research involving human subjects, including surveys of human subjects, must include a statement that the research was performed under appropriate institutional review board oversight.

NIH Public Access Policy

AJVR  is in compliance with the NIH Public Access Policy and with the open access policies of other research funders.

Patient confidentiality and the right to privacy

• Authors have an obligation to protect the personal privacy of patients and clients and to maintain the confidentiality of patient-client information.
• For any manuscript containing patient information (eg, patient descriptions, photographs, or pedigrees) that would allow specific animals or their owners to be identified, the authors must obtain a signed statement of informed consent to publish the information (in print and online) from the owners. Generally, such consent should include an opportunity for the owner to read the manuscript to be submitted for publication. If necessary, nonessential identifying data can be removed, unless clinically or epidemiologically important. However, identifying data may not be altered or falsified.
• Cropping or altering photographs to remove nonessential identifying information is acceptable, so long as the photographs are not otherwise altered. Patient identifiers may not appear in photographs.
• Authors must also obtain informed consent to publish from any identifiable person appearing in photographs. Importantly, these guidelines also apply to any materials (eg, text, photographs, or videos) submitted for posting as supplementary materials.

Prior publication

• Manuscripts are considered with the understanding that they have not been published previously and will not be submitted for publication elsewhere while under consideration by AJVR .
• Manuscripts submitted to the journal following or prior to presentation at a scientific meeting or publication of preliminary findings elsewhere, such as in abstract form, will be considered for publication. Publication of abstracts and posting of slides or videos of the scientific presentation in a print or online conference proceedings is acceptable.
• Posting preprints of research manuscripts in progress or in submission on a public server is not considered prior publication and is also acceptable. Preprints are defined as research manuscripts that have not undergone formal peer review by a journal. Preprints may also be posted at any time during the review process.
• Authors should disclose preprint posting, including DOI, URL link, and license details, at the time of manuscript submission or during the review process. Authors are requested to update any prepublication versions with a link to the final published article.
• Media coverage of presentations at scientific meetings will not jeopardize consideration, but direct release of results beyond what was included in the abstract through press releases or news media briefings may preclude consideration of the manuscript by this journal.

Plagiarism Policy

At the time of submission, similarity detection software (iThenticate) is used. If the similarity score is less than 25%, no further action is taken. If it’s over 25%, iThenticate is run again with title, author list, affiliations, methods, and references hidden from the check. If it’s still over 25%, authors are informed of the findings and directed to revise the language or provide a reference if the text is recycled from one of their previously published articles.

For Librarians

AJVR ceased print production in June 2022 and became fully open access as an online-only journal. You and your patrons may now access AJVR online, which includes copyedited, typeset articles before they are assembled into an issue as well as all online content from 2000 to the present.

One of the AVMA’s strategic initiatives for the journal is the digitization of pre-2000 content ( AJVR was launched in 1940). In 2024, you will have free access to the digital archive of the 1990s JAVMAs, and it is our plan to digitize 1 decade of archival content each of the next 3 years going back to the 1970s. For further information about the archive digitization, please see Editor-in-Chief Dr. Lisa Fortier’s November 2023 editorial .

AVMA is committed to the longterm preservation of all content published in our journals, whether open access or subscribed ( JAVMA only). We collaborate with digital archive provider CLOCKSS, a sustainable, geographically distributed dark archive that builds on the LOCKSS enabling technology.

If your institution is interested in negotiating a “Read and Publish” agreement with AVMA, whereby for a single annual fee your faculty, students, and staff gain free access to JAVMA and waiver of open access article processing charges (CC BY-NC license), please contact Editor-in-Chief Dr. Lisa Fortier at [email protected] for further information.

AJVR is owned and published by the AVMA.

How to Submit

General information

To submit manuscripts, please visit https://mc.manuscriptcentral.com/ajvr . Note, an article processing charge of $1,250 will apply to accepted manuscripts. For author and presubmission inquiries, please contact [email protected] . For details on AVMA news, conferences, or other AVMA information, please visit avma.org .

Submission checklist—ensure the following:

• If accepted, the authors agree to pay an open access article processing charge of $1,250.
• Email address.
• Full postal address.
• The manuscript has been checked for spelling and grammar.
• Abbreviations and preferred pharmacologic and pharmacokinetics terminology, pulmonary and respiratory terminology, and radiographic projection naming are used in accordance with journal guidelines .
• All references mentioned in the reference list are cited in the text.
• Footnotes are not used (review the instructions on citing special materials for information on citing materials previously included as footnotes).
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• Manuscript.
• Figures (include relevant captions), with the same figure names (eg, Figure 1) as used in the manuscript.
• Supplementary files (where applicable).
• Why is this study important? (2 or 3 sentences)
• What did you find? (2or 3 sentences)
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Instructions for Journal Award Nominations

       .

The annual AVMA Journal Awards recognize contributions of students, interns, and residents for manuscripts published in JAVMA or AJVR . Nominations are accepted year-round.

Qualifications

• Nominee is the first or corresponding author
• Work supporting the manuscript was performed when the nominee was a student, intern, or resident
• Manuscripts are assessed for potential impact and clinical relevance, and winners are selected by an Associate Review Board Panel

Nomination Instructions

• Nominations close on March 15 for the preceding year
• Name of nominee
• Nominee category (student, intern, or resident)
• Attestation sentence confirming that the work was done by the nominee
• Manuscript title and name of the journal ( JAVMA or AJVR )
• Nominee’s current email address
• Winners will be announced during the AVMA Annual Convention
• Certificate of achievement
• Cash prize of $500
• Extensive social media exposure
• A waiver of open access fees ($1,250 value) for each winner’s next paper in JAVMA or AJVR  

Click on an issue cover below to read more. For information about images on the cover, contact Stacey Geelan at [email protected] .

May 2022 AJVR Vol. 83 - Issue 5

This month’s cover image displays a multi-omics approach for machine learning (ML) in veterinary medicine. The last decade has brought tremendous growth in the use of artificial intelligence (AI) in veterinary medicine. This has been accompanied by rapid evolution in -omics technologies in veterinary practice. With the use of ML technologies, the combination of these disparate -omics datatypes, like genomics, image features (radiomics), and radiation dose distribution features (dosiomics), offers the potential for a deeper understanding of diseases, their detection and expression, and better treatments for them. This image was obtained as part of the “Currents in One Health Study.” Am J Vet Res . 2022;83(5):385–392. DOI: 10.2460/ajvr.22.03.0038

April 2022 AJVR Vol. 83 - Issue 4

The cover illustration depicts the oromaxillofacial region as a potential and promising model for a ‘One Health’ global approach in regenerative medicine and tissue engineering. Translating discoveries of regenerative therapeutics in veterinary species to novel, effective human therapies using a One Health approach provides a fundamental link between basic biomedical research and medical clinical practice, with the goal of developing strategies for curing or preventing disease and ameliorating pain and suffering in companion animals and humans alike. Am J Vet Res . 2022;83(4):291–297. DOI: 10.2460/ajvr.21.12.0208

March 2022 AJVR Vol. 83 - Issue 3

This month’s cover images show blood smears from a Jack Russell Terrier presented with very severe pulmonic stenosis (peak systolic trans-stenotic = 340 mmHg). On the blood smear before valvuloplasty (A), severe schistocytosis (arrowheads) and polychromasia (asterisk) are present, suggestive of regenerative hemolytic anemia due to abnormal blood flow. The schistocytosis completely resolved 2 months after pulmonary balloon valvuloplasty (B). This study concluded that poikilocytosis (schistocytosis, acanthocytosis, keratocytosis) is common in dogs with congenital ventricular outflow tract obstruction associated with hemolytic anemia in a few cases with severe or very severe pulmonic stenosis. These results indicate the importance of serial blood smear analysis from canine patients with severe or very severe ventricular outflow tract obstruction. The images were obtained as part of the following study: Passavin P, Chetboul V, Poissonnier C, et al. Red-blood cell abnormalities occur in dogs with congenital ventricular outflow tract obstruction. Am J Vet Res . 2022;83(3):198–204. DOI: 10.2460/ajvr.21.11.0188

February 2022 AJVR Vol. 83 - Issue 2

This month's cover image shows the bacterial pathobiome map of canine oral health and disease. Dogs were assigned to one of four different stages of periodontal disease and their microbiome was analyzed. Several bacterial species were identified that were significantly associated with the different stages of periodontitis. This pathobiome map can provide a guidance to veterinarians to understand their patient’s periodontal microbiome and health status. The image was obtained as part of the following study: Niemiec BA, Gawor J, Tang S, Prem A, Krumbeck JK. The bacteriome of the oral cavity in healthy dogs and dogs with periodontal disease. Am J Vet Res . 2022;83(1):50–58. DOI: 10.2460/ajvr.21.02.0027

January 2022 AJVR Vol. 83 - Issue 1

This month's cover images are shear-wave elastographic images of the color map and propagation map of the mandibular lymph node from a healthy adult beagle to measure shear-wave velocities (SWVs). This study concluded that SWE could be used to non¬invasively evaluate changes in the elastic properties of lymphatic tissues. Ultimately, SWE could be applied to increase the diagnostic accuracy of tumor staging. Intra- and interobserver reliability were excellent suggesting that SWV of lymph nodes could be used in practice settings.

The images were obtained as part of the following study: Kang YR, Lee SH, Seo IM, et al. Comparison of shear-wave velocities obtained with shear-wave elastography of various peripheral lymph nodes in healthy Beagles. Am J Vet Res . 2021;82(12):981–987. DOI: 10.2460/ajvr.82.12.981

December 2021 AJVR Vol. 82 - Issue 12

This month's cover (top photomontage) depicts a series of sodium fluorescein angiographic images exhibiting various degrees of vascular abnormalities (dye leakage) present within the iris of diabetic canines. A standard color, near-infrared, indocyanine green and sodium fluorescein angiographic image of an age-matched control is also shown below. Venular dilation, in the absence of dye leakage, is illustrated. The presence and severity of vascular changes (dye leakage) noted in diabetic canines appear to be associated with disease duration and blood glucose regulation. Anterior segment angiography could be employed to identify the severity of these vascular disruptions present, in addition to, monitoring disease progression and potential response to therapeutic interventions. These images were obtained as part of the following study: Pirie CG, Shelnutt LM, Langlois DK. Assessment of iris vasculature abnormalities in dogs with diabetes mellitus. Am J Vet Res 2021;82(10):829–839. doi: 10.2460/ajvr.82.10.829

November 2021 AJVR Vol. 82 - Issue 11

This month's cover images are micro-CT and photomicrographs of proximal sesamoid bones (PSB) of Thoroughbred racehorses following catastrophic PSB fracture. Osteophyte size at the apical and basilar margins was scored and found to positively correlate with accrued total career high-speed furlongs and with catastrophic PSB fracture. The image was obtained as part of the following study: Cresswell EN, Ruspi BD, Wollman CW, et al. Determination of correlation of proximal sesamoid bone osteoarthritis with high-speed furlong exercise and catastrophic sesamoid bone fracture in Thoroughbred racehorses. Am J Vet Res 2021;82:467–477.

October 2021 AJVR Vol. 82 - Issue 10

This month's cover image is a photograph of multiple cross sections of the prostate gland from a canine cadaver in which microwave ablation with cooling urethral perfusion was performed in 2 zones (white outline and arrow). The median percentage of prostate gland ablation for this and 7 other cadavers in this group was 73%, and urethral mucosal injury was limited. The image was obtained as part of the following study: Traverson M, Lin S, Kendall A, et al. Investigation of the use of microwave ablation with and without cooling urethral perfusion for thermal ablation of the prostate gland in canine cadavers. Am J Vet Res 2021;82:395–404.

September 2021 AJVR Vol. 82 - Issue 9

This month's cover image is a composite photograph of the bottom of an equine foot that was shod with 5 different types of shoes to determine which one provided the best hoof protection and traction for horses on paved surfaces. Results suggest that shoes coated with a thin layer of tungsten carbide (Panel E), and plastic-steel composite shoes (Panel F) offered the best protection and traction. The image was obtained as part of the following study: Wang P, Takawira C, Taguchi T, et al. Assessment of the effect of horseshoes with and without traction adaptations on the gait kinetics on nonlame horses during a trot on a concrete runway. Am J Vet Res 2021;82:292–301.

August 2021 AJVR Vol. 82 - Issue 8

Echocardiographic image obtained from a modified apical 4-chamber view used to measure right atrial longitudinal strain (RALS) indices (left), and curves obtained from 120 healthy dogs (right). The right atrial wall is divided into 6 color-coded segments, and the colors correspond to the strain curves, with the average color in white.The authors concluded that age should be considered in the interpretation of RALS indices in clinical settings. Morita T, Nakamura K, Osuga T, et al. Repeatability, reproducibility, and reference intervals for indices of right atrial longitudinal strain derived from speckle-tracking echocardiography in healthy dogs. Am J Vet Res 2021;82:274–279.

July 2021 AJVR Vol. 82 - Issue 7

This month's cover image is a photograph of a mature (> 18 months old) domestic laying hen ( Gallus domesticus ) during ovariectomy performed with a vessel and tissue–sealing device through a left lateral celiotomy. Notice the ovary with multiple follicles and yolks of various sizes. The image was obtained as part of the following study: Sullivan JL, Wakamatsu N, Yin J-H et al. Assessment of a vessel and tissue–sealing device for ovariectomy in chickens to evaluate the potential application of the procedure to other avian species. Am J Vet Res 2021;82:310–317.

June 2021 AJVR Vol. 82 - Issue 6

This month's cover image is a photograph depicting explants harvested from fibrocartilage on the dorsal surface of the deep digital flexor tendon of the forelimb of a horse. To obtain these explants, the navicular bone was dissected from the foot en bloc, and the deep digital flexor tendon segment directly opposing the navicular bone was harvested with aseptic technique. The explants were obtained with a 4-mm biopsy punch. The image was obtained as part of the following study: Sullivan SN, Cole SL, Stewart MC, et al. Ex vivo effects of corticosteroids on equine deep digital flexor and navicular fibrocartilage explant cell viability. Am J Vet Res 2021;82:125–131.

May 2021 AJVR Vol. 82 - Issue 5

This month's cover image is a photomicrograph depicting the results of immunohistochemical staining for insulin expression in isolated canine insulinoma cells after 48 hours of culture. Insulin content (red stain) varies among individual insulinoma cells (arrowheads). Intact insulinoma cells approximately 10 µm in diameter are enlarged after processing with the cytocentrifuge. The image was obtained as part of the following study: Suwitheechon O, Schermerhorn T. Evaluation of the expression of hexokinase I, glucokinase, and insulin by canine insulinoma cells maintained in short-term culture. Am J Vet Res 2021;82:110–117.

April 2021 AJVR Vol. 82 - Issue 4

This month's cover image is a photomicrograph of a section of placentome obtained at 80 days of gestation from a pregnant yearling Katahdin ewe that was experimentally inoculated with 4 X 107 TCID50 of a noncytopathic bovine viral diarrhea virus type lb isolate (NY-I strain), intranasally, at 65 days of gestation. The chorioallantois is lined by plump trophoblastic epithelium that contains multifocal areas of intracytoplasmic immunoreactivity for bovine viral diarrhea virus antigen (red-stained tissue). The image was obtained as part of the following study: Lear A, Pohler K, Sula M-JM, et al. Alterations in pregnancy-associated glycoprotein concentrations of pregnant sheep experimentally infected with bovine viral diarrhea virus. Am J Vet Res 2021;82:63–70.

March 2021 AJVR Vol. 82 - Issue 3

This month's cover image is a photomicrograph of a section of liver tissue from a dog that was euthanized because of development of clinical signs of liver failure 12 weeks after being treated with lomustine (75 mg/m2, PO, q 21 d for 5 doses). Notice that the portal vein profiles are diminished, prominent pigmented macrophages are present in the portal tract and adjacent parenchyma, and persistent biliary hyperplasia is evident. The image was obtained as part of the following study: Dedeaux AM, Flesner BK, Reinhart JM, et al. Biochemical, functional, and histopathologic characterization of lomustine-induced liver injury in dogs. Am J Vet Res 2020;81:810–820.

February 2021 AJVR Vol. 82 - Issue 2

This month's cover image is a laparoscopic image obtained during microwave ablation of the ovary in a healthy dog. The dog was positioned in dorsolateral recumbency for the procedure, and laparoscopic forceps were used to grasp the proper ligament and elevate and stabilize the ovary. The microwave ablation probe was placed percutaneously through the ventrolateral aspect of the abdominal wall at an orientation parallel to the long axis of the ovary after complete dissection of the ovarian bursa. Notice how dissection of the ovarian bursa improved the visibility of the ovary and facilitated correct positioning of the probe in the central longitudinal axis of the ovary. The image was obtained as part of the following study: Verpaalen VD, Case JB, Dark MJ, et al. Feasibility and efficacy of ultrasonographic and laparoscopic guidance for microwave ablation of clinically normal canine ovaries. Am J Vet Res 2020;81:747–754.

January 2021 AJVR Vol. 82 - Issue 1

This month's cover features color-coded transverse abdominal MRI images illustrating hepatic apparent diffusion coefficient and hepatic fat fraction in a healthy neutered male cat (top) and a neutered male cat that underwent 40 weeks of dietary intervention to achieve and maintain a body condition score of 7/9 (bottom). Notice the bright violet component in the liver and increased subcutaneous and visceral fat tissue in the bottom cat. The color scale bar depicts approximate percentage fat. The image was obtained as part of the following study: Steger G-L, Salesov E, Richter H, et al. Evaluation of the changes in hepatic apparent diffusion coefficient and hepatic fat fraction in healthy cats during body weight gain. Am J Vet Res 2020;81:796–803.

December 2020 AJVR Vol. 81 - Issue 12

This months cover image is a perfusion CT image of the abdomen of a healthy Beagle. A region of interest (dotted circle) has been placed over the body of the pancreas, and blood flow is expressed as a color map. The image was obtained as part of the following study: Park S, Jung J-W, Je H, et al. Effect of slice thickness on computed tomographic perfusion analysis of the pancreas in healthy dogs. Am J Vet Res 2020;81:732–738.

November 2020 AJVR Vol. 81 - Issue 11

This month's cover image is a photomicrograph of a section of neoplastic lymph node from a dog illustrating expression of luteinizing hormone receptors, as determined with an immunohistochemical, LH receptor–specific stain and hematoxylin counterstain. The image was obtained as part of the following study: Ettinger AM, Gust SK, Kutzler MA. Luteinizing hormone receptor expression by nonneoplastic and neoplastic canine lymphocytes. Am J Vet Res 2019;80:572–577.

October 2020 AJVR Vol. 81 - Issue 10

This month's cover image consists of a series of median plane T2-weighted spin-echo magnetic resonance images and corresponding anatomic sections of cervical intervertebral disks in cadaveric cats. From left to right, the images illustrate increasing stages of intervertebral disk degeneration. The images were obtained as part of the following study: Alisauskaite N, Bitterli T, Kircher PR, et al. Evaluation of agreement and correlation of results obtained with MRI-based and macroscopic observation-based grading schemes when used to assess intervertebral disk degeneration in cats. Am J Vet Res 2020;81:309–316.

September 2020 AJVR Vol. 81 - Issue 9

This month's cover image is a photomicrograph depicting qualitative histologic scoring of neutrophil infiltration (in this instance, rare neutrophils, or a score of 1 on a scale from 1 to 5) at local injection sites in equids after SC administration of ReqIL-4, ReqIL-8, or a negative control solution, each mixed with a biopolymer carrier. A 6-mm-diameter biopsy punch was used to collect cutaneous and subcutaneous tissues, including the biopolymer plug, at the injection sites. The biopolymer is pink, and the host tissue is saffron-yellow to orange. The image was obtained as part of the following study: Godbout M, Vargas A, Hélie P, et al. Use of a biopolymer delivery system to investigate the influence of interleukin-4 on recruitment of neutrophils in equids. Am J Vet Res 2020;81:344–354.

August 2020 AJVR Vol. 81 - Issue 8

This month's cover image is a lateromedial radiographic view illustrating locations of soft tissue attachments in the tarsal region of horses. Illustrated locations represent composite findings based on dissection and radiographic imaging of 8 hind limbs from healthy adult horses. The image was obtained as part of the following study: Casillas JM, Jacobs CC, Manfredi JM. Radiographic localization of the attachments of soft tissue structures in the tarsal region of horses. Am J Vet Res 2020;81:406–415.

July 2020 AJVR Vol. 81 - Issue 7

This month's cover features an endoscopic image of the coelomic cavity in a male bearded dragon ( Pogona vitticeps ). The endoscope was inserted in a cranial direction through an incision just lateral to the umbilicus. In this image, the gallbladder can be seen between lobes of the liver. The location of the skin incision for the ventral approach was chosen to avoid the ventral abdominal vein and pelvic veins. The image was obtained as part of the following study: Frei S, Sanchez-Migallon Guzman D, Kass PH, et al. Evaluation of a ventral and a left lateral approach to coelioscopy in bearded dragons ( Pogona vitticeps ). Am J Vet Res 2020;81:267–275.

June 2020 AJVR Vol. 81 - Issue 6

This month's cover image is a transverse computed tomographic image of the cranial abdominal region in a healthy dog showing placement of regions of interest for evaluation of perfusion of the pancreas (T1 and T2) and liver (T3 and T4). Colors indicate the scale from the lowest (blue) to highest (red) amount of perfusion. The image was obtained as part of the following study: Kloer TB, Rao S, Twedt DC, et al. Computed tomographic evaluation of pancreatic perfusion in healthy dogs. Am J Vet Res 2020;81:131–138.

May 2020 AJVR Vol. 81 - Issue 5

This month's cover image is a 5-chamber view obtained by use of multidetector computed tomography of the left side of the heart in a healthy adult hound-type dog. The lumen of the left atrium is shaded pink. The image was obtained as part of the following study: Owens EJ, LeBlanc NL, Scollan KF. Comparison of left and right atrial volumes determined by two- and three-dimensional echocardiography with those determined by multidetector computed tomography for healthy dogs. Am J Vet Res 2020;81:33–40.

April 2020 AJVR Vol. 81 - Issue 4

This month's cover image is an endoscopic image (with the endoscope positioned in a caudal-to-cranial direction) of the abdomen of a rabbit obtained during evaluation of working space achieved with CO2 insufflation to an intra-abdominal pressure of 8 mm Hg. The diaphragm and liver are shown. The image was obtained as part of the following study: Kabakchiev CM, zur Linden AR, Singh A, et al. Effects of intra-abdominal pressure on laparoscopic working space in domestic rabbits ( Oryctolagus cuniculus ). Am J Vet Res 2020;81:77–83.

March 2020 AJVR Vol. 81 - Issue 3

This month's cover image is a contrast-enhanced ultrasonographic image of the left kidney of a Beagle obtained at the time of peak enhancement. Notice the placement of 3 regions of interest (black circles) in the renal cortex and 2 regions of interest (white circles) in the renal medulla. Each region of interest area was 0.11 cm 2 . The image was obtained as part of the following study: Hwang J, Kang K, Kang J, et al. Effect of catheter diameter and injection rate of flush solution on renal contrast-enhanced ultrasonography with perfluorobutane in dogs. Am J Vet Res 2019;80:825–831.

February 2020 AJVR Vol. 81 - Issue 2

This month's cover image is a dorsal-plane, maximum-intensity CT image, displayed in a bone window, of a cat after injection of contrast medium into a popliteal lymph node. Notice the caudal extent of thoracic duct branching. No other abnormalities were identified during evaluation of baseline CT lymphangiography images. The image was obtained as part of the following study: Dickerson VM, Grimes JA, Secrest SA, et al. Abdominal lymphatic drainage after thoracic duct ligation and cisterna chyli ablation in clinically normal cats. Am J Vet Res 2019;80:885–890.

January 2020 AJVR Vol. 81 - Issue 1

This month's cover image consists of a 3-D model (left) and a heat map (right) illustrating deviations from the articular surface of the radius of a feline cadaver. The 3-D model illustrates positive differences between models created by use of laser-scanned images and images obtained with CT in a transverse orientation. For the heat map, negative deviations (light to dark blue) indicate that the CT model overestimated the articular surface, and positive deviations (yellow, orange, or red) indicate that the CT model underestimated the articular surface. The image was part of the following study: Webster CE, Marcellin-Little DJ, Koballa EM, et al. Evaluation of the geometric accuracy of computed tomography and microcomputed tomography of the articular surface of the distal portion of the radius of cats. Am J Vet Res 2019;80:976–984.

December 2019 AJVR Vol. 80 - Issue 12

This month's cover image is a photograph of the ground surface of a bovine hind foot with toe-tip necrosis. The photograph was obtained during application of a static load of 3 kN, and a region-growing technique involving a stylus and tablet was used to process the image to highlight regions of white line separation (yellow areas). The image was part of the following study: Johnston JD, Eichhorn DJR, Kontulainen SA, et al. Investigation of white line separation under load in bovine claws with and without toe-tip necrosis. Am J Vet Res 2019;80:736–742.

November 2019 AJVR Vol. 80 - Issue 11

This month's cover image is an illustration of the cranial aspect of a bovine stifle joint depicting placement of a prosthesis for extracapsular stabilization in cattle with cranial cruciate ligament rupture. For this prosthesis, 800-lb-test monofilament nylon leader line would be threaded through bone tunnels and secured with three 316 stainless steel crimping sleeves on the lateral aspect of the joint. The illustration was created as part of the following study: Lozier JW, Niehaus AJ, Hinds CA, et al. Investigation of a novel prosthesis technique for extracapsular stabilization of cranial cruciate ligament-deficient stifle joints in adult cattle. Am J Vet Res 2019;80:779–786. ©The Ohio State University, Tim Vojt.

October 2019 AJVR Vol. 80 - Issue 10

This month's cover image is a photomicrograph of a section of neoplastic lymph node from a dog illustrating expression of luteinizing hormone receptors, as determined with an immunohistochemical, LH receptor–specific stain and hematoxylin counterstain. The image was obtained as part of the following: Ettinger AM, Gust SK, Kutzler MA. Luteinizing hormone receptor expression by nonneoplastic and neoplastic canine lymphocytes. Am J Vet Res 2019;80:572–577.

September 2019 AJVR Vol. 80 - Issue 9

This month's cover image is a volume rendering of the intracranial arteries in a 21-month-old 5-kg mixed-breed dog with idiopathic epilepsy; the image was obtained in the dorsal plane by use of 1.5-T, 3-D, time-of-flight magnetic resonance angiography. Bilaterally, the caudal communicating artery (green) and rostral cerebellar artery (pink) overlap the internal carotid artery (blue), which is located ventral to the caudal communicating artery. The image was obtained as part of the following study: Ishikawa C, Ito D, Tanaka N, et al. Use of three-dimensional time-of-flight magnetic resonance angiography at 1.5 Tesla to evaluate the intracranial arteries of 39 dogs with idiopathic epilepsy. Am J Vet Res 2019;80:480–489.

August 2019 AJVR Vol. 80 - Issue 8

This month's cover features an echocardiographic image obtained during longitudinal strain analysis in a dog with tricuspid valve dysplasia. The image shows a left apical 4-chamber image that was analyzed with 2-D speckle tracking echocardiography software. The left ventricle is outlined in red by the software. The image was obtained as part of the following study: Santarelli G, Toaldo MB, Bouvard J, et al. Variability among strain variables derived from two-dimensional speckle tracking echocardiography in dogs by use of various software. Am J Vet Res 2019;80:347–357.

July 2019 AJVR Vol. 80 - Issue 7

This month's cover image represents a propagation map of the right liver lobe of a healthy dog obtained by means of 2-D shear wave elastography in elasticity mode. Notice the consistent parallel contour lines. The image was obtained as part of the following study: Tamura M, Ohta H, Nisa K, et al. Evaluation of liver and spleen stiffness of healthy dogs by use of two-dimensional shear wave elastography. Am J Vet Res 2019;80:378–384.

June 2019 AJVR Vol. 80 - Issue 6

This month's cover image is a photomicrograph of a skin sample obtained from the incision site of a koi 2 weeks after coelioscopy. The image depicts poor healing; the epithelium is incomplete, and the wound surface is partially covered by a layer of serofibrinous crust and cellular debris. The sides of the incision have separated, and severe pyogranulomatous inflammation extends transmurally through the section, which severely disrupts the normal tissue architecture. Suture material is visible. The image was obtained as part of the following study: Stevens BN, Sanchez-Migallon Guzman D, Phillips KL, et al. Evaluation of diagnostic coelioscopy in koi ( Cyprinus carpio ). Am J Vet Res 2019;80:221–229.

May 2019 AJVR Vol. 80 - Issue 5

This month's cover image is a photomicrograph of a portal region in a section of a hepatic biopsy sample from a cat with lymphocytic cholangitis. Bile duct proliferation has been made obvious through immunohistochemical staining of proliferative epithelial progenitors. The image was obtained as part of the following study: Chandler AM, Center SA, Randolph JF, et al. Reference limits for hepatic bile duct-to-arteriole and bile duct-to-portal tract ratios in healthy cats. Am J Vet Res 2019;80:15–23.

April 2019 AJVR Vol. 80 - Issue 4

This month's cover image is a representative 3-D reconstruction of the entire renal volume in a healthy dog. The image was created by combining CT attenuation values of the entire renal volume (including renal parenchyma) in all transverse planes. The image was obtained as part of the following study: Wallimann M, Richter H, Sieber-Ruckstuhl NS, et al. Influence of injection protocol and measurement technique on computed tomographic assessment of glomerular filtration rate in healthy Beagles. Am J Vet Res 2018;79:1298–1305.

March 2019 AJVR Vol. 80 - Issue 3

This month's cover image was generated during finite element modeling depicting the minimum principal strain at the bone-pin interface for a 2 transfixation pin–cast construct applied to an equine forelimb. The insert represents a higher magnification of the bone-pin interface outlined by the white line in the main image. Notice that local bone strain at the bone-pin interface was quite high, reaching approximately –15,000 microstrain. The image was obtained as part of the following study: Thomas KL, Carmalt JL, Burnett WD, et al. In vitro mechanical evaluation of three transfixation pin–cast constructs applied to equine forelimbs. Am J Vet Res 2018;79:1287–1297.

February 2019 AJVR Vol. 80 - Issue 2

This month's cover image is a photomicrograph of equine lamellar tissue following extracorporeal perfusion of the limb with lipopolysaccharide. The section was immunostained for total (active and latent) matrix metalloproteinase-9 (brown). Notice the moderate reaction in fibroblasts, endothelial cells, and suprabasal epidermal cells and intense reaction in basal epidermal cells. The image was obtained as part of the following study: Patan-Zugaj B, Gauff FC, Egerbacher M, et al. Endotoxin-induced changes of type VII collagen–cleaving matrix metalloproteinases in lamellar tissue of extracorporeally perfused equine limbs. Am J Vet Res 2018;79:986–994.

January 2019 AJVR Vol. 80 - Issue 1

This month's cover image is a scanning electron micrograph of the surface of a cast stainless steel tibial plateau leveling osteotomy plate that had been explanted from a dog. Notice the circular areas of corrosion that can be seen between scratches on the surface of the plate. The image was part of the following study: Sprecher CM, Milz S, Suter T, et al. Retrospective analysis of corrosion and ion release from retrieved cast stainless steel tibia plateau leveling osteotomy plates in dogs with and without peri-implant osteosarcoma. Am J Vet Res 2018;79:970–979.

December 2018 AJVR Vol. 79 - Issue 12

This month's cover image is a sagittal proton-density fast spin echo image of the supraspinatus tendon of a nonlame 2-year-old sexuallty intact male Beagle. Notice that the collagen-dense fibers of the superficial and deep margins of the supraspinatus tendon have a hypointense signal, whereas the thick water-rich central substance has a hyperintense signal. The image was obtained as part of the following study: Pownder SL, Caserto BG, Hayashi K, et al. Magnetic resonance imaging and histologic features of the supraspinatus tendon in nonlame dogs. Am J Vet Res 2018;79:836–844.

November 2018 AJVR Vol. 79 - Issue 11

This month's cover image is a photomicrograph of cultured primary canine aortic endothelial cells. Endothelial cells were grown to confluency and fixed in 4% paraformaldehyde, then stained for vascular endothelial–cadherin with rabbit polyclonal anti-CD144 antibody. Fluorescent-labeled goat anti-rabbit lgG was used as the secondary antibody, and cell nuclei were stained with blue fluorescent bisbenzimide stain. The image was obtained as part of the following study: König ML, Lettry SC, Marti E, et al. Validation of a human angiopoietin-2 ELISA for measurement of angiopoietin-2 concentrations in canine plasma samples and supernatant of primary canine aortic endothelial cell cultures. Am J Vet Res 2018;79:803–810.

October 2018 AJVR Vol. 79 - Issue 10

This month's cover image is a photograph of the left eye of a 24-year-old female horse that was obtained during anterior segment indocyanine green angiography. The image was obtained 18 seconds after injection of indocyanine green and illustrates filling of the radial iris veins. The image was part of the following study: Pirie CG, LoPinto AJ, Tenney WA. Comparison of angiographic dyes and injection techniques for ocular anterior segment angiography in horses. Am J Vet Res 2018;79:562–567.

September 2018 AJVR Vol. 79 - Issue 9

This month's cover image is a color-coded map created by postprocessing of delayed gadolinium-enhanced magnetic resonance images of the distal sagittal medial midcondylar region of the second phalanx of a horse obtained after injection of gadolinium into the dorsal recess of the distal interphalangeal joint. Different colors represent different cartilage relaxation times, with light green and blue pixels representing articular cartilage. The image was obtained as part of the following study: Bischofberger AS, Fürst AE, Torgerson PR, et al. Use of a 3-Telsa magnet to perform delayed gadolinium-enhanced magnetic resonance imaging of the distal interphalangeal joint of horses with and without naturally occurring osteoarthritis. Am J Vet Res 2018;79:287–298.

August 2018 AJVR Vol. 79 - Issue 8

This month's cover image consists of photomicrographs of representative cytologic preparations of neutrophils isolated from equine blood samples and cultured for 12 hours with (top) and without (bottom) lipopolysaccharide (1 µg/mL). Treatment with LPS delayed apoptosis of neutrophils for up to 24 hours. The image was obtained as part of the following study: Anderson SL, Townsend HGG, Singh B. Role of toll-like receptor 4 and caspase-3, -8, and -9 in lipopolysaccharide-induced delay of apoptosis in equine neutrophils. Am J Vet Res 2018;79:424–432.

July 2018 AJVR Vol. 79 - Issue 7

This month's cover image is a transverse CT image of a clinically normal Holstein calf. The calf was anesthetized and received an IV injection of iodinated contrast medium, with dynamic CT scanning of the head initiated at the time of contrast medium injection. The image was created by merging a perfusion CT color map of cerebral blood flow with a conventional CT image of the same slice, and was obtained as part of the following study: Kishimoto M, Kushida K, Yamada K. Perfusion computed tomographic measurements of cerebral blood flow variables in live Holstein calves. Am J Vet Res 2018;79:177–180.

June 2018 AJVR Vol. 79 - Issue 6

This month's cover image is a photomicrograph depicting the distribution of Helicobacter -like organisms on the mucosal surface of the gastric antrum in a healthy dog. The image was obtained as part of the following study: Suárez-Esquivel M, Alfaro-Alarcón A, Guzmán-Verri C, et al. Analysis of the association between density of Helicobacter spp and gastric lesions in dogs. Am J Vet Res 2017;78:1414–1420.

May 2018 AJVR Vol. 79 - Issue 5

This month's cover image is a photograph from the caudal aspect of a canine cadaver larynx after bilateral ventriculocordectomy performed via ventral laryngotomy. The image was obtained as part of the following study: Regier PJ, McCarthy TC, Monnet E. Effect of bilateral ventriculocordectomy via ventral laryngotomy on laryngeal airway resistance in larynges of canine cadavers. Am J Vet Res 2017;78:1444–1448.

April 2018 AJVR Vol. 79 - Issue 4

This month's cover image is a photomicrograph of a tissue section obtained from a horse 120 days after unicortical ostectomy of the third metacarpal bone and filling of the defect with a castor oil polyurethane polymer containing calcium carbonate. There is a degenerating clot on the bone tissue in a pore of the polymer and focal areas of bone matrix. The image was obtained as part of the following study: Nóbrega FS, Selim MB, Arana-Chavez VE, et al. Histologic and immunohistochemical evaluation of biocompatibility of castor oil polyurethane polymer with calcium carbonate in equine bone tissue. Am J Vet Res 2017;78:1210–1214.

March 2018 AJVR Vol. 79 - Issue 3

This month's cover image is a photomicrograph of equine hematopoietic cells after culturing for B-cell differentiation and immunostaining with antibodies against B-cell signature transcription factor E2A/TCF3 (green) and cell surface molecule CD19 (red); nuclei were stained blue with 4',6-diamidino-2-phenylindole. The image was obtained as part of the following study: Schwab UE, Tallmadge RL, Matychak MB, et al. Effects of autologous stromal cells and cytokines on differentiation of equine bone marrow–derived progenitor cells. Am J Vet Res 2017;78:1215–1228.

February 2018 AJVR Vol. 79 - Issue 2

This month's cover features an endoscopic image of the upper respiratory tract of a horse after nasopharyngeal administration of contrast medium. The contrast medium is visible on the surfaces of the nasopharynx, laryngopharynx, and larynx. The image was obtained as part of the following study: Colbath AC, Valdés-Martinez A, Leise BS, et al. Evaluation of two methods for topical application of contrast medium to the pharyngeal and laryngeal region of horses. Am J Vet Res 2017;78:1098–1103.

January 2018 AJVR Vol. 79 - Issue 1

This month's cover features a photomicrograph of a cytologic preparation of bone marrow from a healthy juvenile female New Zealand White rabbit showing large granulocyte precursor cells. The myeloid precursor cells are 2 to 3 times the diameter of mature heterophils. The image was obtained as part of the following study: Riedel RM, de Matos R, Schaefer DMW. Bone marrow cell composition and morphology in healthy juvenile female New Zealand White rabbits ( Oryctolagus cuniculus ). Am J Vet Res 2017;78:910–918.

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National Research Council (US) Committee on the National Needs for Research in Veterinary Science . Critical Needs for Research in Veterinary Science. Washington (DC): National Academies Press (US); 2005.

Critical Needs for Research in Veterinary Science.

• Hardcopy Version at National Academies Press

2 Progress and Opportunities in Veterinary Research

T his chapter outlines some of the contributions of veterinary research and the promise it holds for the improvement of public health and food safety, animal health, and the advancement of comparative medicine. Because animal welfare—defined as the well-being of individual animals, that is, normal functioning and freedom from disease and injury—is an extension of animal health that involves veterinary research, it is appropriate to include it in the field of animal health. Research in several subdisciplines of the three fields has been identified as critical for advancing and protecting animal and human health. In addition, several emerging issues span two or more fields, so they cannot be neatly categorized as subdisciplines of public health and food safety, animal health and welfare, or comparative medicine ( Box 2-1 ). Although the different aspects of veterinary research are grouped under four headings—public health and food safety, animal health and welfare, comparative medicine and emerging issues, they are intertwined. For example, research in comparative medicine contributes to animal health through development of preventive medicine and treatment. Study of wildlife diseases contributes not only to wildlife health and conservation but also to the study of emerging infectious diseases, many of which are zoonotic.

Subdisciplines of Veterinary Research that are Critical to Improving Public Health and Food Safety, and Animal Health and Advancing Comparative Medicine. Public Health and Food Safety Food Safety

• PUBLIC HEALTH AND FOOD SAFETY

Food Safety

Foodborne illnesses, as defined by the World Health Organization, are diseases—usually infectious or toxic—caused by agents that enter the body through the ingestion of food. They are a major cause of human morbidity and mortality in the United States, responsible for an estimated 76 million illnesses, 325,000 hospitalizations, and 5,000 deaths a year ( Mead et al., 1999 ). Animals—both domesticated and wild—are frequent reservoirs of foodborne pathogens that can cause human illness and animals are among the most common vehicles of enteric bacterial infections in humans ( http://www.cdc.gov/foodnet/ ). For example, more than 70% of sporadic Campylobacter infections in the United States have been associated with eating foods of animal origin or contact with animals ( Friedman et al., 2004 ). Eating contaminated poultry products is largely responsible for cases of Salmonella enteritidis infection ( Kimura et al., 2004 ). Escherichia coli O157:H7 infections are associated principally with eating products of bovine origin, contact with ruminants, and consumption of water contaminated with bovine feces ( Kennedy et al., 2002 ; Kassenborg et al., 2004 ). Primary risk factors for multiple drug-resistant Salmonella newport infections are contact with cattle and consumption of bovine products ( Gupta et al., 2003 ). Most of those microorganisms are commensals that reside in the animal gastrointestinal tract and cause no apparent symptoms of illness and had no adverse effects on weight gain or milk or egg production. Most foodborne pathogen infections have no effect on animal health or on economic factors associated with animal production so there has been considerably less emphasis on veterinary food-safety research than on research to improve animal health, and greater advances have been made in controlling diseases of livestock and poultry than in reducing the occurrence of pathogens in these animals.

Animal-associated pathogen contamination of food occurs both before and after harvest. Livestock and poultry are the primary sources of many harmful microorganisms that are transmitted to foods and on the farm during harvesting, slaughter, and processing. Such foods as meat and poultry can be directly contaminated with pathogens through contact with animal manure during production and processing, and other foodstuffs, such as fruits and vegetables, can be indirectly contaminated through the environment, for example via irrigation water tainted with livestock manure. Various determinants influence the carriage and transmission of foodborne pathogens during animal production and processing. For example, an animal's diet can affect the microbial composition of its intestinal tract and can serve as a source of harmful agents, such as prions related to bovine spongiform encephalopathy from ruminant neurological tissue; in free-range conditions, outdoor exposure to wildlife presents a greater opportunity for transmission of indigenous pathogens of vermin, pests, and wild animals than does controlled indoor housing, which largely excludes vermin and wild animals; in some times of the year, such as summer, there is a dramatic increase in pathogen carriage by livestock and poultry; shipping of animals can induce stress and greater susceptibility to pathogen shedding; and slaughtering practices, such as cold-water chilling of poultry, can disseminate pathogens among carcasses during processing. There are many gaps in our understanding of where in the production and processing chain interventions will have the greatest effect on reducing pathogen loads and ultimately providing the greatest public-health protection.

Of the estimated 76 million cases of foodborne illness that occur each year in the United States, CDC estimates that there are 62 million cases of food-associated acute gastroenteritis of unknown etiology ( Mead et al., 1999 ). Although the causative agents of many well-documented foodborne outbreaks of distinctive illness (such as Brainerd diarrhea) remain unknown ( Bean et al., 1996 ), many new foodborne pathogens were identified in recent years. For example, during the late 1970s and early 1980s, three major bacterial pathogens were first identified by microbiologists and public-health epidemiologists as agents of foodborne illness: Campylobacter jejuni, E. coli O157:H7, and Listeria monocytogenes . Since then, C. jejuni has become recognized as the leading cause of acute bacterial gastroenteritis in many developed countries, E. coli O157:H7 has been identified as the leading cause of hemolytic uremic syndrome, and L. monocytogenes has become a primary cause of death among recognized foodborne pathogens. It is possible that many emerging foodborne pathogens are not newly evolved but already exist in nature and have yet to be identified or associated with foodborne disease.

Resistance to antimicrobial agents has become a major public health concern and subtherapeutic use of antibiotics as growth promoters in animals has been alleged to be one of the major factors in antibiotic resistance. Arguments for and against that association have been presented ( Turnidge, 2004 ; Phillips et al., 2004 ), but surveillance systems that monitor the distribution, occurrence, and trends in numbers of antimicrobial-resistant pathogens in humans, animals, and environmental sources will be critical for resolving this issue. The United States, in 1996, created the National Antimicrobial Resistance Monitoring Systems (NARMS), which was a collaborative effort of the Center for Veterinary Medicine in the Food and Drug Administration (FDA), the US Department of Agriculture (USDA), and the Centers for Disease Control and Prevention (CDC). Major contributions to the field of antimicrobial resistance among pathogens of animal and human significance have been achieved through elucidation of mechanisms of development of antimicrobial resistance. The principal genetic force responsible for induction of antibiotic resistance in bacteria has been found to be horizontal gene transfer of plasmids, transposons, and integrons. Although it is known that the emergence and dissemination of bacterial antimicrobial resistance result from numerous complex interactions among antimicrobials, microorganisms, and the surrounding environment, the relative importance of specific factors in mobilization of these genetic factors between organisms is unknown.

The genetics revolution has led to potential introduction of desirable characteristics in food-producing animals, such as developing transgenic lines of food animals intrinsically resistant to traditional foodborne pathogens. However, methods used to modify animals genetically may introduce compositional changes, some of which may be undesirable. Potential hazards include toxicity, allergy, nutrient deficiencies and imbalances, and risks associated with endocrine activity. Research is needed to assess the safety and nutritional values of transgenic and cloned food animals.

Continuing veterinary research on food safety is needed to improve detection and surveillance of foodborne pathogens associated with livestock and poultry production, define the ecology of foodborne pathogens in food-producing animals and their environment, develop interventions to reduce the dissemination of foodborne pathogens by poultry and livestock, study the development and mechanisms of antibiotic resistance of foodborne pathogens associated with animals in the food chain, and develop methods to assess the safety and nutritional value of transgenic and cloned animals.

Examples of Critical Research Needs

• Rapid, sensitive, and accurate assays for detecting foodborne pathogens.
• Epidemiological approaches to identifying risk factors and intervention strategies that have the greatest effect on reducing foodborne pathogens and antimicrobial-resistant microorganisms associated with livestock, poultry, and aquaculture. This includes a more comprehensive understanding of the epidemiology and genetic elements of the foodborne zoonotic agents, especially of those agents that have recently emerged.
• Practical and effective interventions for minimizing carriage of and contamination with food-associated pathogens of animal origin.
• Methods to assess the safety and nutritional value of transgenic and cloned food-producing animals.
• Identification of previously unrecognized foodborne pathogens of animal origin.

Importance and Contribution of Research

The US Department of Agriculture (USDA) Economic Research Service estimates more than $15 billion in annual medical expenses and lost productivity resulting from salmonellosis, Campylobacter enteritis, and enterohemorrhagic E. coli infections alone ( USDA-ERS 2004 ). A concerted research effort to address food safety can prevent the recurrence and reduce the effects of the more than 3.5 million estimated cases of foodborne illness each year of which livestock and poultry are the primary sources of causative agents. Veterinary research will contribute to eliminating transmission of pathogens to foods of animal origin.

Biosecurity

Biosecurity is the integrated system of policies, training, and procedures designed to deter, interdict, detect, respond to, and recover from intentional introduction of biological agents or related products that can cause disease or death in humans, animals, or plants. Until 1997, almost all US research done for the purpose of developing countermeasures to biological warfare was done in the Department of Defense (DOD) ( Zajtchuk, 1997 ). Veterinarians with board certification in laboratory animal medicine or comparative pathology or with doctoral degrees in specialty fields—such as physiology, pharmacology, toxicology, and microbiology—played an important role in that research. Research conducted at the US Army Medical Research Institute of Infectious Disease (USAMRIID) at Fort Detrick, MD—the lead DOD laboratory for medical biological defense— and in other laboratories led to important vaccines, drugs, and diagnostics for military personnel. Other government departments became involved in biodefense research—first the Centers for Disease Control and Prevention (CDC) in 1998 and then the National Institute of Allergy and Infectious Diseases (NIAID) in 2002. Several medical countermeasures developed at USAMRIID—such as cell-culture-derived smallpox vaccine and recombinant anthrax vaccine—have now been moved into advanced development by the Department of Health and Human Services.

Although food safety has been an integral part of veterinary medicine throughout history, food biosecurity is an emerging issue that affects the entire food chain. Preharvest biosecurity research is concerned with protection of animal health and production, and postharvest biosecurity research is related to food microbiology and toxicology (refer to food safety section above). Examples of agricultural and food-biosecurity research being conducted by veterinary scientists include the development of preharvest and postharvest surveillance systems, diagnostic and detection systems, vaccines, immunomodulating drugs, animal and product tracking systems, and ecologically sound means of disposal of animal carcasses.

A new awareness of the need for food and agricultural biosecurity research arose after September 11 and the “anthrax letter” attacks of 2001 because biosecurity research is closely related to maintaining a safe agricultural sector and food supply. The US food and fiber industry generates over $200 billion a year in farm cash receipts ( USDA, 2003 ). From an economic standpoint, adulteration of food could alter market sentiment through fear and thus have substantial economic impact with enormous potential ripple effects. Furthermore, sequential or multifocal attacks on our food supply could undermine the trust of the American people in their government.

Agricultural bioterrorism and the vulnerability of the food-producing animal industries in the United States to such activity are addressed in a National Research Council report ( NRC 2003a ). That report provides an in-depth analysis of the known agents that could be used to disrupt food-animal production and discusses the research and infrastructure needed to develop countermeasures. The fact that animals cannot be easily protected from the group of diseases suggested as primary agents of agricultural bioterrorism is indicative that those conditions should be among those given high priority for veterinary research. (See Appendix D for list of bioterrorism agents.) In 2004, the Department of Homeland Security (DHS) awarded 3-year grants to two university consortia to study preharvest and postharvest agricultural biosecurity ( DHS 2004 ). In addition, several academic centers—typically in land-grant universities—have established their own centers, and some have or are constructing biosafety level 3 laboratories in which to conduct agricultural research (see Appendix E ).

• Improved ability to detect and identify disease and pathogens in animal populations.
• Improved ability to detect pathogens and toxicants in food along the processing chain.
• Improved understanding of interactions between pathogens and hosts so that effective preventive measures and countermeasures can be developed.
• Rational development of cost-effective countermeasures, both vaccines and nonspecific therapeutic agents.

Although veterinary researchers are already addressing important research issues related to agricultural terrorism and emerging disease, we are slowly gaining an appreciation of the importance of integrating human and animal health issues through “species-neutral” disease surveillance ( Box 2-2 ) and of combining findings internationally rather than only nationally.

Species-Neutral Disease Surveillance. Species-neutral surveillance is defined as monitoring diseases of all animal species, including humans and domestic and wild animals, and communicating the findings throughout the health-care community. Animal health (more...)

• ANIMAL HEALTH AND WELFARE

Food-Producing Animals

Food-producing animals include all species of mammals and birds (including wildlife) that are raised in captivity or domestic conditions primarily as sources of human food. Research on infectious diseases and noninfectious health problems of metabolic or genetic origin in food-producing animals has been going on for many years, conducted by a combination of veterinary and nonveterinary medical scientists and animal scientists. Much of the knowledge of nutrition, metabolism, and nutritional deficiencies that applies to humans was discovered as a result of observations on animals. Although frank clinical symptoms of specific nutrient deficiencies are rare today in food-producing animals because of the extensive knowledge of nutrient requirements, research on food and feed is needed because it represents the largest cost associated with handling food-producing animals.

As genetic modifications in animals are made and metabolic manipulation is imposed through pharmaceuticals to enhance or focus production, it will be increasingly important to meet the nutrient needs of these “harder-working” animals. (Safety of genetically modified animals as food is discussed in the “ Public Health and Food Safety ” section above.) Historically, such efforts focused on diseases that affected single animals or individual herds or flocks and addressed issues associated with production, such as reproductive diseases, nutritional deficiencies, and mammary gland infections; but zoonotic diseases, such as tuberculosis and brucellosis, and their eradication were also of great concern. New information, vaccines, and technologies have led to continued advances in understanding and improved early detection, prevention, control, and eradication. The success of that work has helped the United States to become the largest source of food-producing animals. Such contemporary issues as the increasingly important subject of zoonotic diseases have shown the need for new approaches to ensuring the health and well-being of food animals. Food-animal production is often near areas occupied by wildlife or other domestic species (such as companion animals), which can contribute to the transmission of zoonotic or other diseases. This is a complex issue that requires expertise in comparative medicine and epidemiology. (See also the sections in this chapter on “ Animal Health and Welfare ” under subsection “ Wildlife and Conservation ” and on “ Emerging Issues ” under subsection and “ Emerging Infectious Diseases ” for discussions of zoonotic disease transmission.)

Examples of the importance attached to those needs are found in recent documents published by the National Research Council ( NRC 2002b ). Emerging animal diseases and their effect on markets and the economy and on global animal and human health and safety have been addressed, with emphasis on foot-and-mouth disease (FMD) and bovine spongiform encephalopathy (BSE), (the international concerns of the time), each of which had an enormous economic impact. The cost of BSE in the United Kingdom in direct compensation was reported to be in billions of US dollars in 2002 ( NRC 2003b ), and there were substantial additional effects in international markets. The cost to North American cattle markets has been estimated at $3 to $5 billion. To add to the seriousness of the BSE and general prion issues, it has recently been reported that some people may act as subclinical carriers of variant Creutzfeldt-Jakob disease (vCJD) ( Carrell 2004 ; Head and Ironside 2005 ); that BSE has been naturally transmitted to goats ( Anon. 2005 ) and an array of zoo animals, including kudus, antelopes, and cheetahs ( Kirkwood and Cunningham, 1994 ); and that in naturally infected captive greater kudu, BSE prions have an unprecedented wide distribution throughout tissues ( Cunningham et al., 2004 ). The reports suggest that the impact of vCJD may be difficult to predict, that the potential host range for the BSE prion is very wide, and that transmission to humans or other animals through novel pathways is possible. In the case of FMD, a disease not known to be transmitted to humans (and thus primarily an issue of animal health and economics), the estimated cost in the United Kingdom in 2001 has been set at $30 billion ( NRC 2003b ).

• Development of capacity and implementation of broad programs in comparative medicine to understand, rapidly detect, and control zoonotic and nonzoonotic diseases in food-producing animals raised in concentrated production units, with emphasis on techniques and technologies for field use in large animal populations.
• Evaluation of the implications of increases in productivity achieved through genetic or pharmaceutical means for animal health, nutrient, and metabolic requirements.
• Monitoring and assessment of trans-species disease transmission, epidemiology, and the delineation of resistance, susceptibility, and virulence factors across animals and pathogenic organisms.

A thorough understanding of diseases in food animals would improve our ability to detect diseases rapidly and control them effectively. Otherwise, the food-producing animal system will continue to be vulnerable to disease outbreaks with major consequences for animal health and the economy. Failure to address the issues above noted will erode the ability of the food-animal industries of the United States to be globally competitive and economically viable and will subject them to the potential devastation created by natural or human-made biodisasters. A critical issue on the global level is the understanding, detection, and control of the various diseases that are associated with prions (such as BSE and CJD).

Aquaculture

For the purposes of this report, aquaculture is defined as the farming of aquatic animals including finfish (such as salmon and catfish) and shellfish (such as clams, mussels, and shrimp). Freshwater catfish production dominates aquaculture in the United States and generates about $1 billion per year. Marine aquaculture—involving primarily salmon, clams, and shrimp—represents about one-third of aquaculture production by weight. From 1989 to 1998, there were marked increases in the aquaculture production of catfish (by 40%), salmon (468%), clams (379%), and shrimp (193%) ( Goldburg et al., 2001 ).

Aquaculture has only recently involved veterinary research. Increases in the quantity and economic importance of farmed species and in intensive production practices, have led to a rising need for disease detection, treatment, and prevention. Veterinary researchers have contributed substantially to the identification and characterization of important aquaculture diseases, such as infectious salmon anemia ( Kibenge, et al., 2004 ). In addition, scientists of the FDA Center for Veterinary Medicine have been conducting studies on the effectiveness of treatment of fungal infection and internal parasites in fish ( FDA 2003 ).

• Improved understanding of immune responses (especially cell-mediated) in fish to facilitate the development of effective vaccines and appropriate delivery systems.
• Improved methods of pathogen detection.
• Increased effectiveness and safety of medications used to treat diseases in aquaculture species.
• Enhanced understanding of the impact of aquatic animal production systems on marine and freshwater ecosystems.

Lack of effective disease identification, prevention, and control strategies (for example, efficacious vaccines) in aquaculture species results in the overuse of antibiotics and chemicals. Overuse leads to economic losses (for example, high mortality in fish production facilities), human health hazards (for example, compromised food safety because of drug or pollutant residues and zoonotic pathogens) ( Benbrook, 2002 ), and adverse environmental effects (for example, antimicrobial and pesticide use, and transmission of disease to wild populations).

Companion Animals

Over the last several decades, veterinarians and animal scientists have contributed to advancing the diagnosis and treatment of disease and to the understanding of companion-animal welfare and the human-animal bond ( Badylak et al., 1998 ; Dodds, 1995a , b ; Lawrence, 1994 ; Ostrander et al., 1993 ; Parker et al., 2004 ; Patterson et al., 1988 ; Smith, 1994 ). Advances in companion-animal research have led to markedly increased expectations for animal and human medical services ( Lawrence, 1994 ; Eyre et al., 2004 ). The breadth and sophistication of veterinary diagnostic and treatment methods have increased the need for timely high-quality research ( Boothe and Slater, 1995 ; Smith, 1994 ; Dodds, 1995a ).

Research involving companion animals has been conducted by many investigators at a wide array of institutions and organizations. Companion animal-research has typically been in three categories: research on the diseases or conditions of companion animals for their direct benefit, research on diseases of comparative medical or pathological significance that provides direct benefits to companion animals and indirect benefits to humans, and research on basic physiological, pharmacological, molecular, or pathological processes that primarily benefits humans but benefits companion animals indirectly. (See section on Comparative Medicine for details on animal models for biomedical research.) Basic-science researchers, pathologists, and clinicians have all made useful contributions to companion-animal research.

The scope of companion-animal research has increased considerably over the last several decades. There are still important disease-related problems in most of the traditional medical disciplines (for example, pharmacology, immunology, pathology, internal medicine, orthopedics, cardiology, oncology, and ophthalmology), but attention is increasingly directed at emerging matters related to animal welfare (such as quality-of-life determination and animal abuse), animal-shelter medicine and control of unowned and feral animal populations, the human-animal bond (including the role of service animals), complementary medicine, and the cause and treatment of behavioral disorders.

Companion animals play important roles in service work, not only in assisting people with special needs but also in herding, search and rescue, drug and chemical detection, police and military assistance, and hunting and retrieving. Research into the behavioral and training needs of this special group of companion animals will increase their quality of life and enhance their performance as assistants, protectors, and life-savers.

Horses have historically been used as companion animals and performance. Therefore, equine research has been directed primarily at improving overall health and soundness by developing diagnostic screening tests for heritable traits and studying the causes of common debilitating diseases, such as laminitis (founder) and exercise-induced pulmonary hemorrhage. Of specific importance to the viability of some horse breeds is the need to restrict breeding of horses that carry deleterious genetic traits. For example, hyperkalemic periodic paralysis in quarterhorses can be traced to one famous foundation sire, combined immunodeficiency of Arabians is traceable to a particular group of animals, and the lethal white gene of paint foals is produced by matings of the overo-to-overo color pattern.

Companion-animal research improved the health of animals and humans by the enhanced control of infectious diseases through vaccines (such as distemper, parvovirus, and rabies), development of pharmaceutical agents, and the study of disease processes (such as retroviral disease; comparative hematology, immunology, and oncology; and animal models of human disease).

Epidemiological studies of animal populations historically have been directed primarily to public health and control of infectious diseases. More recently, comparative epidemiologists and geneticists have turned their attention to studying populations of related animals to identify biochemical markers that can be used in screening for genetic diseases and to performing health surveys to more accurately describe the health problems affecting the population as a whole. The goals have been to learn more about diseases and to reduce the number of affected and carrier animals ( Dodds, 1995b ; Patterson et al., 1988 ; Smith, 1994 ). The widely appreciated screening programs include those for hip and elbow dysplasia; inherited blood, cardiac, thyroid, and eye diseases; and congenital deafness. Many infectious agents can be transmitted to humans from companion animals (for example, Toxoplasma gondii ) and some organisms have the potential for bi-directional transmission (for example, methicillin-resistant Staphylococcus aureus ) ( Weese, 2005 ). The proximity of humans and their companion animals increases the need to understand diseases that may be passed between them.

• Preventive-medicine and wellness strategies—vaccination and other means to control infectious disease, appropriate nutrition, methods or strategies for disease monitoring, and better methods for diagnosing and treating behavioral disorders.
• Improved understanding of and treatment for geriatric and immune disorders—such as cancer, organ failure, arthritis, and immune-mediated disease.
• Rapid and minimally invasive diagnostic methods.
• Randomized controlled clinical trials (of sufficient power to detect clinically significant differences) to address many long-standing diagnostic and treatment questions.
• Concentrated efforts in reproductive efficiency and orthopedic issues of performance animals.
• Improved understanding of the ecology of microbial organisms that may be transmitted to humans from companion animals and vice versa.

Failure to address issues involving companion-animal health and well-being will result in substantial morbidity and mortality in companion-animal populations; adversely affect the psychological well-being of their owners and the family and social framework; and delay or prevent advances in pharmaceutical and biologics development and in the understanding and treatment of many important human and animal diseases.

Companion-animal health research will improve the length and quality of life for companion animals, which in turn will have favorable effects on their caregivers. Such research will also provide valuable comparative-disease information that will benefit human and animal health.

Laboratory Animals

Laboratory animals are integral to our understanding of basic biology and physiology and have contributed to the discovery and development of virtually every human and animal health product and technique used in contemporary medical practice. The sophisticated specialty of laboratory animal medicine has evolved over the years to provide expertise in the breeding, management, and humane care of research animals and expertise in experimental design and methodology. Laboratory animal veterinarians have also provided leadership in developing national standards for laboratory animal care, use, facilities, and housing.

Some valid nonanimal alternatives have been developed for research, testing, and education, but the advancement of biological and medical knowledge will continue to depend on whole-animal models (primarily rats and mice) which represent the complex interactions between organ systems. Moreover, recent advances in genomics and proteomics will probably require an increase in the number of animals used in research (Lancet, 2004). Recent predictions ( NRC, 2003b ) suggest that the number of mice used in research in the United States will increase by 10-20% a year from 2000 and 2010. If this is true, more than 200 million mice and rats will be used each year in the United States by the end of this decade. As the number of animals used in research increases, the demand for high quality, well-defined animal models is likely to intensify. To meet that need, additional research and new methods to ensure animal health and well-being are required.

The credibility of the data generated from animal research depends in large part on the quality of laboratory animals with regard to their health status and genetic integrity, the quality of their environment and care, and how they are handled. Reproducible research requires that animal subjects be maintained in a stable environment to minimize experimental variables. For more than 50 years, the need for reliable experimental animal models has driven advances in their health quality and care. However, naturally occurring viral, bacterial, and parasitic infections continue to be detected in institutional rodent colonies throughout the United States. The adverse effect of such infectious diseases on the quality of research is well established. For example, mouse parvovirus infection affects the immune system and therefore may confound studies involving immune system functions ( McKisic et al., 1993 , 1996 ). The presence of Helicobacter species in the intestinal flora of laboratory mice may influence the research in pathogenesis of inflammatory bowel disease and other gastrointestinal disease ( Sadlak et al., 1993 ; Kullberg et al., 2003 ). Infections often lead to disruption of the research process until the disease is eradicated from the rodent colony. Better methods for preventing the introduction of pathogens and the development of more specific and sensitive methods of disease detection are required to minimize the potential for variables and to ensure the validity of research data.

Relatively few published, peer-reviewed scientific studies support or refute the effects of cage or pen size or environmental enrichment on animal well-being. Few research studies have addressed the optimal frequency of cage changes or pen sanitation. Even the Guide for the Care and Use of Laboratory Animals , on which most of the housing standards and sanitation practices used in contemporary animal facilities are based, acknowledges that research on laboratory animal management continues to generate scientific information that should be used in evaluating performance and engineering standards. It also recognizes that for some issues, insufficient information is available and continued research into improved methods of animal care and use is needed. Research into those factors, the effects of noise levels and frequency, and optimal environmental temperature and humidity at the cage or pen level is needed for different species and strains.

In accordance with the Public Health Service policy on the Care and Utilization of Vertebrate Animals used in Testing, Research, and Training, appropriate animal care and use includes the “avoidance or minimization of discomfort, distress, and pain when consistent with sound scientific practices.” Procedures that may cause more than momentary or slight pain or distress should be performed with “appropriate sedation, analgesia, or anesthesia,” and “animals that would otherwise suffer severe or chronic pain or distress that cannot be relieved should be painlessly killed at the end of the procedure or, if appropriate, during the procedure.” The assessment and management of pain and distress are often based on the laboratory animal veterinarian's training, knowledge, judgment, and experience with the various laboratory species. However, much of what we know about animal pain is extrapolated from human requirements, which may not be appropriate for all species or for individual animals. Studies are needed to assess and manage pain and distress in laboratory animals and to provide guidance for humane end points for animal-research protocols.

Although the use of whole-animal models is expected to increase in the foreseeable future, development of valid alternatives should be included among the scientific community's long-term goals. USDA regulations and Public Health Service (PHS) policy require scientists to consider alternatives, including reduction in the number of animal used, to refine techniques to prevent or minimize pain or distress, or to use in vitro methods before initiating an animal-research protocol. Several federal regulatory and research agencies, under the auspices of the Interagency Coordinating Committee on the Validation of Alternative Methods and the National Toxicology Program Interagency Center for the Evaluation of Alternative Toxicological Methods, are working on the development, validation, acceptance, and national and international harmonization of toxicity testing methods.

• Prevention, detection, and management of laboratory animal diseases.
• Laboratory animal management standards and practices—including the identification of optimal cage and pen sizes, environmental enrichment, sanitation, noise, and temperature and humidity—based on research data.
• Assessment and management of pain and distress.

Valid alternatives to reduce, refine, or replace animal testing.

Additional research on infectious diseases is needed to understand how they affect the quality of research data and to guide disease management. Gaps in our knowledge of laboratory animal care and housing requirements must also be addressed through sound scientific research and should be used to develop and implement standards of care. To enhance animal welfare, studies are needed to identify optimal methods for pain assessment and management and test systems that reduce, refine, or replace the use of animals.

Wildlife and Conservation

Wildlife diseases have three important implications for society. First, anthropogenic activities continue to bring humans closer to wildlife so transmission of zoonotic diseases from wildlife to humans and domestic animals or vice versa is of increasing concern. Second, wildlife populations are increasingly at risk for diseases that cause severe population declines, which in turn may affect ecosystem health. (See section on “ Emerging Issues ” in this chapter.) Third, harvested wildlife is culturally and economically important in many regions of the United States, and captive wild animals in zoological collections are invaluable national assets for education, conservation, and our cultural understanding of wildlife.

Veterinary researchers' involvement in wildlife biology originally stemmed from the need to support the health of hunted or captive wildlife. More recently, veterinary researchers have been active in studying diseases that affect endangered species in the wild and developing techniques to treat and control the spread of disease in wildlife populations. Veterinary researchers in wildlife diseases have contributed to our understanding of and management of disease effects on wild and captive populations—for example, brucellosis in bison, tuberculosis transmission between deer and cattle populations in the upper Midwest, and Mannheimia sp. transmission from domestic sheep and goats to bighorn sheep.

Wildlife diseases can have important consequences for our economy. For example, chronic wasting disease (CWD) is a spongiform encephalopathy similar to BSE, which emerged in the United Kingdom and cost over $100 billion in lost cattle production and outbreak control ( NRC 2002b ). CWD was seen first in the late 1960s in captive mule deer and then in the 1980s in free-ranging deer and elk in northeastern Colorado and southeastern Wyoming. Confirmed cases have been found in at least eight more states, including Wisconsin and New Mexico. In 2002, Wisconsin reported first cases of CWD in deer ( Wisconsin DNR, 2002 ). The economic costs of CWD are due largely to depopulation, loss of hunting-license revenue, and huge efforts by affected and unaffected states in surveillance monitoring and diagnostics. CWD cost Wisconsin $10 million and Colorado $19 million in 2002 alone ( Bishop, 2002 ).

The importance of veterinary research to hunted wildlife species has led to increased veterinary research activities in state and federal agencies. USDA, under the Animal and Plant Health Inspection Service, conducts veterinary research on wildlife species in its Veterinary Services section and its Wildlife Services section. The US Fish and Wildlife Service undertakes a number of wildlife veterinary research activities as part of its mission. In 1975, the US Geological Survey National Wildlife Health Center was set up to assess the effects of disease on wildlife with particular reference to wildlife losses, especially on federal land, of migratory species or federally listed endangered species.

Over the last few decades, many of those agencies have begun to shift their agendas to veterinary research on nongame wildlife. The shift has occurred in response to outbreaks of infectious disease that have become widely recognized by scientists and the public as threats to survival of wildlife species ( Box 2-3 ). The conservation effect of wildlife diseases has been highlighted in a series of mass deaths ( Daszak et al., 1999 , 2003 ), some of which were linked to species extinction ( Daszak et al, 2000 ). Infectious diseases and the ecological factors that cause them to emerge are a threat to the conservation of biodiversity.

Examples of Wildlife Disease Outbreaks. Canine distemper in black-footed ferrets and plague in their prairie dog prey base. Chytridiomycosis, a newly discovered fungal diseases that led to widespread decline in amphibians.

The shift of veterinary research away from hunted species was a response to the effects of pollution on wildlife or of illness with unknown etiologies. For example, amphibians have undergone severe population declines in some regions, including in parts of the Rocky Mountains and other regions of the United States. The discovery of a fungal disease responsible for amphibian population declines highlights a role for veterinary researchers in understanding such phenomena ( Berger et al, 1998 ). The causative fungal disease is now recognized as a major threat for global amphibian extinction ( Green and Sherman, 2001 ).

In addition to conservation, veterinary researchers can play a role preventing transmission of wildlife diseases between agricultural and other animal species. Brucellosis in bison in the greater Yellowstone ecosystem poses a risk to ranched cattle, and recent canine distemper viral infections in more than 100 domestic dogs, raccoons, and zoo animals in the Chicago area have been attributed to an initial outbreak in raccoons ( Lednicky et al., 2004 ; R.D. Schultz, personal communication, December 3, 2004).

Besides free-ranging wildlife, captive wildlife in zoos also provide opportunities to examine the important interfaces among domestic animals, free-ranging wildlife, and humans. Historically, studies of animals in zoological collections have yielded important discoveries and advances in animal and human medicine. For example, spontaneously occurring hepatitis in woodchucks was used to further the understanding of the pathogenesis of a form of hepatitis in humans, and the appreciation of the importance of dietary estrogens in wild and domestic animals has been enhanced by studies in zoos. A group of novel molecules important in local defense against microbial invasion were discovered first by studies of captive frogs.

Zoos are increasingly concerned with in situ management and conservation of wild species and their habitats. Many large zoos in the United States have veterinary clinicians on staff, and some have teams of veterinarians and veterinary researchers that study diseases and reproduction in captive and wild animals. Notable discoveries made by zoo veterinary researchers include the discovery that herpes viruses that are benign in Asian elephants can be lethal to African elephants when the two coexist in zoos (work conducted at the US National Zoological Park) and the first demonstrated case of a pathogen's causing extinction of a Partula snail species (at the Zoological Society of London).

A number of zoos now have extensive research programs on wildlife diseases outside their collections, both at home and abroad. They include research on the use of bushmeat, the origins of some zoonoses and socioeconomic connections between human and wildlife health (Wildlife Conservation Society), formation of interinstitutional partnerships to link wildlife and public health (Brookfield Zoo), and avian health studies in the Galapagos (St. Louis Zoo).

A group of institutions related to zoos is wildlife-rehabilitation centers that take in native wildlife to help foster their recovery and release into their native habitats. Rehabilitation centers sometimes use veterinary researchers and clinicians to manage the health of the wildlife populations in their care. Wildlife rehabilitation centers also conduct research on free-ranging wildlife populations. For example, the Marine Mammal Center in California has published key papers on domoic acid poisoning of free-living marine mammals, and the Wildlife Center of Virginia has led research on aural abscessation of native turtles.

Events such as the emergence of West Nile virus and monkeypox and bioterrorism incidents involving zoonotic agents have focused attention on zoos and wildlife-rehabilitation centers. West Nile virus first came to public attention in the United States in 1999, but the virus clearly had been found in captive birds at the Wildlife Conservation Society in New York during the early stages of the outbreak in wild birds and probably before it was found in humans ( Lanciotti et al, 1999 ). Thus, wildlife or captive wild species can act as sentinels for emerging diseases or even bioterrorism agents. Efforts to form networks of zoo veterinarians and wildlife rehabilitators to develop such sentinel capacity are under way (for example, the West Nile virus surveillance program led by Lincoln Park Zoo, which includes the Wildlife Center of Virginia and other rehabilitation centers).

• Research on the risk of transmission of zoonotic and other emerging diseases between wildlife, domestic animals, livestock, and humans.
• Research on wildlife diseases that affect both game and nongame species.
• Assessment of the mechanisms for disease introduction and spread in the United States via trade or natural movement of wildlife populations.
• Research to establish diagnostic criteria and protocols, and to validate and standardize protocols.
• Development of improved tools for detection and controlling diseases in free-ranging wildlife populations.
• Research on conservation including comparative reproduction, assisted reproduction, contraception, habitat restoration and protection, and on reintroduction of captive wildlife.
• Comparative pharmacology and nutrition, including the study of improved anesthetics, antimicrobials, and vaccines.

Wildlife research can reduce the economic impact on states substantially by preventing the spread of diseases in hunted or game species (for example, CWD) and the transmission of wildlife diseases to agricultural animals and humans. Such research can also contribute to the prevention of emerging zoonotic diseases. The veterinary research outlined must be accomplished to prevent population declines in wildlife species that are of interest for ecological balance, recreation, tourism, or conservation and to prevent the emergence of potentially serious pathogens in humans. Veterinary research in zoos is critical to conservation of endangered wildlife, providing unique insights into disease processes in captive animals that can be extrapolated to free-living wild populations.

• COMPARATIVE MEDICINE

Animal Modeling

Animal models for human diseases.

Research on animal models has been essential to our understanding of basic and applied sciences and has led to important improvements in the management of human and animal diseases ( NRC, 2004a ; see Box 2-4 for medical advances achieved through animal research). Over the last 50 years, the study of naturally occurring or induced animal models of human disease has led to tremendous growth of knowledge in many disciplines—including hematology, immunology, vaccinology, virology, and genetics—and has contributed to new fields of research, such as transplantation and gene therapy ( Badylak et al., 1998 ; Dodds, 1995a , b ; Ostrander et al 1993 ; Parker et al., 2004 ; Patterson et al., 1988 , Smith, 1994 ).

Medical Advances Achieved Through Animal Research. 1790 Vaccine for smallpox developed (cow) 1880 Vaccine for anthrax developed (sheep)

Over 90% of the animals used in biomedical research are mice and rats. However, many other animal models have been used to study human and animal diseases. For example, the field of comparative immunology deals with many aspects of immunological function, which includes not only the clinical disorders, such as systemic and organ-specific autoimmune diseases and primary and secondary immune deficiency states, but also understanding of host-parasite interactions and the immunological effects of genetics, nutrition, and toxicity on disease expression ( Perryman, 2004 ; Tizzard and Schubot, 2000 ). Swine have been used in atherosclerosis and hemostasis research ( Bowie and Dodds, 1976 ; Dodds, 1982 , 1987 ; Edwards et al., 1985 ). Pregnancy immunology is studied in ruminants to investigate embryonic survival, fetal growth, and uterine defense mechanisms; and artificial-organ and organ-xenograft research, development, and testing have used and continue to use sheep, cattle, and goats ( Chiang et al., 1994 ; Dodds, 1987 ; Lewis and Carraway, 1992 ; Martini et al., 2001 ). Nonhuman-primate research has long played a key role in comparative research on atherosclerosis, respiratory disease, retroviral diseases, infectious hepatitis, and aging ( Clarkson et al., 1996 ; McClellan, 2000 ; NRC, 1997 ).

Animal Models for Animal Diseases

Information generated by animal-based experiments has been used primarily to benefit human health and well-being, but parallel benefits have been accorded to animals ( Dodds, 1995a ; Wagner, 1992 ); for example, with respect to inherited bleeding disorders ( Dodds, 1995b ), congenital cardiac disease and inborn errors of metabolism ( Patterson et al., 1988 ), neuromuscular and copper-storage disorders (Brewer et al., 1992), and inherited eye diseases ( Smith, 1994 ). These basic and comparative medical advances have improved diagnosis and treatment in clinical veterinary medicine.

Emerging Areas of Research in Comparative Medicine

Molecular markers for research and clinical applications.

For 4 decades, veterinary and comparative geneticists have developed and relied on biochemical markers of specific genetic traits to identify carrier and affected animals can be used as models of human disease ( Patterson et al., 1988 ; Dodds, 1995a , b ; Dodds and Womack, 1997 ). More recently, molecular approaches have been developed used to study gene-therapeutic approaches for advancing human health and well-being ( Ostrander et al., 1993 ). Examples of diseases that commonly affect humans and companion animals and lend themselves to molecular and gene therapy are autoimmune thyroid disease ( Happ, 1995 ), such inherited bleeding disorders as hemophilia and von Willebrand disease ( Dodds, 1995b ; Kay et al., 1993 ), and organ-specific autoimmune disease ( Ford, 2001 ; Schultz, 1999 ). Future technological developments, particularly in gene delivery and cell transplantation, will be critical for the successful practice of gene therapy ( Dodds and Womack, 1997 ).

Animal Genome and Phenome Research

Substantial advances have been made in sequencing the genomes of humans and other mammalian species. Large-scale genome-sequencing projects have focused on completing the sequencing of the genome of the human ( Freimer and Sabatti, 2003 ), the chimpanzee, the dog ( Parker et al., 2004 ), the cow ( Gibbs, et al., 2002 ), the mouse, the rat and the chicken, several insects, nematodes, fungi, yeast, and bacteria ( AVMA, 2004a ). In the near future, scientists will begin to sequence the genomes of nine more mammals, including the domestic cat, the guinea pig, the rabbit, the orangutan, and the elephant ( AVMA, 2004a ).

Interest in the human and canine genomes has spawned related research in “phenomics” to identify specific genotypes that are associated with the species phenotype. The purpose of the human, mouse, and canine phenome projects is to learn about both genetic and nongenetic factors that contribute to the variability of the species ( Bogue, 2003 ; Freimer and Sabatti, 2003 ; Grubb et al., 2004 ; Pletcher et al., 2004). For example, dog research will focus on the phenotypic characteristics that distinguish one breed from another and that distinguish one animal from another in the same breed. Size, anatomy and appearance, composition and metabolism, behavior and temperament, and disease susceptibility will be investigated.

Effective Animal Models to Establish Safety and Efficacy of Therapeutic Compounds

The challenge today is to develop better treatments for the many serious diseases that afflict human and animal populations. FDA's Critical Path Initiative focuses on targeted scientific efforts to modernize methods to evaluate the safety, efficacy, and quality of medical products as they move from product selection and design to mass manufacture. Critical-path research complements basic research, but results in the creation of new tools for product development. Medical-product development starts with basic research that leads to discovery and prototype design and then proceeds to preclinical trials in animal models to test for efficacy and finally clinical trials and FDA approval. The costs of that process are increasing rapidly, but the failure rate of candidate drugs in clinical development has increased. Extensive use of computer modeling (“silicotechnology”) could improve predictability, shorten time for drug development, and reduce the overall cost of drug development by as much as 50%. Improved data-mining efforts to combine in vitro and in vivo animal studies with human clinical outcomes (while protecting proprietary data effectively) could form the basis of useful predictive safety models.

Animal models have been informative for efficacy and safety studies of new lead compounds and therapeutics, but improvements are still needed. Further characterization of existing and newly developed disease models in rodents and other laboratory animal species will lead to better validation of potential therapeutic disease targets and analysis and understanding of disease pathways in animal models ( Kinkler, 2004 ).

Stem-Cell Research

The goal of stem-cell research is to engineer cell lines for use in tissue, organ, or cell transplantation or for gene therapy for treatment of diseases ( NIH, 2004 ). The future of regenerative medicine depends on further exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of adult and embryonic human and mouse stem cells ( NRC, 2002c ).

Stem-cell transplantation has been effective in treating diseases in animal models. However, although effective outcomes of stem-cell transplantation have been obtained—for example, in neurodegenerative diseases—the underlying mechanisms leading to re-establishment of neurological function are still unclear. Such mechanisms as stem-cell promotion of growth-factor release, cell fusion, and transdifferentiation are some explanations of the favorable outcomes. Additional work with animal models of disease will result in a better understanding of the mechanisms of stem-cell therapies.

Genetically Engineered Animals

The capacity to manipulate the DNA of mammals by adding or deleting specific genes has made the laboratory mouse a robust tool for advancing biomedical research. Genetic engineering has substantially increased the number of mutant strains available compared with induced-mutagenesis methods, such as N-ethyl-N-nitrosourea (ENU) mutagenesis. For example, genetically engineered mouse models have advanced the understanding of such neurodegenerative diseases as Alzheimer's disease, Parkinson's disease, and motor neuron disease ( Wong et al., 2002 ).

Transgenic sheep and goats express foreign proteins in their milk that may be used to treat such genetic defects as human and canine hemophilia. Transgenic pigs may serve as a source of organs for transplantation into humans (xeno-transplantation). Further development of transgenic animals will permit investigations that will eludicidate the cellular components of tissue remodeling that are essential to regenerative medicine.

Advanced Surgical Techniques (Microsurgery) and Biomedical Devices

Research in advanced surgical techniques includes the development of the skills needed for microvascular, microneural, and microtubular surgery, which are used in plastic and orthopedic surgery, urology, general surgery, neurosurgery, otolaryngology, obstetrics and gynecology, and cardiothoracic surgery. Training courses typically use rabbits and rats as experimental models. A research model of arterial thrombosis that mimics human vascular thrombosis (for example, coronary arterial occlusion) has been used extensively by investigators interested in the development of thrombolytic agents, particularly urokinase and tissue plasminogen activators, for human use ( Badylak et al, 1998 ). A biomaterial derived from porcine small intestinal submucosa was developed from a throw-away product of the pork industry; this “bioscaffold” material has been used in a variety of animal models and in human patients for repair, replacement, and reconstruction of the esophagus, dura mater, lower urinary tract, acutely and chronically injured skin, and the cardiovascular system (Badylak et al, 2000).

Vaccine-Related Research

Understanding of basic immune mechanisms in laboratory animals has made it possible to design vaccines that protect against infectious diseases, to induce effective responses to tumor antigens, and to control graft rejection and autoimmune diseases ( Tizard, 1990 ; Lanzavecchia, 1993 ). However, there is an emerging need for new approaches to protect against immunological and infectious challenges ( Cohen, 1994 ) and to understand adverse reactions to vaccines in humans and animals ( Oehen et al., 1991 ; Paul et al., 2003 ; Schultz, 1999 ; Scott-Moncreieff et al., 2002 ; Tizard, 1990 ; Vascellari et al., 2003 ).

• Advanced training of comparative-medicine scientists to support and facilitate biomedical research, with emphasis on expertise in phenotype and behavior assessment of unique rodent strains.
• Further development and refinement of animal models to advance biomedical research.
• Expansion of resources and methods for characterizing the genetic background, phenotype, and behavior of unique mouse and rat strains.
• Enhanced methods for preserving valuable models and improving the reproductive efficiency of laboratory animals.
• Improved methods for genetic engineering in laboratory animal species other than the mouse to advance understanding of select diseases.

Research in comparative medicine is critical to the advancement of biomedical research, which will lead to improvements in human and animal health. Comparative-medicine research contributes to the improved quality of laboratory animals and the quality of research that uses them.

• EMERGING ISSUES IN VETERINARY SCIENCE

Emerging Infectious Diseases

Emerging infectious diseases (EIDs) have become recognized as one of the most important threats to public health over the last 30 years ( Binder et al., 1999 ; IOM, 1992 ; NRC, 2003b ). Emerging diseases are those which have recently expanded in geographic range, moved from one host species to another, increased in impact or severity, or undergone a change in pathogenesis, or were caused by recently evolved pathogens (other definitions are available in IOM, 1992 ). Combating emerging diseases is a key goal of public-health efforts nationally and globally, and it is hindered by poor knowledge of potential emerging zoonoses— for example, diseases caused by wildlife parasites, viruses, and other microorganisms that move into humans ( Morse, 1993 ).

The reason emerging diseases (most of which are zoonotic) require and attract so much attention is that they are usually complex and not well understood, are not susceptible to rapid diagnostic or detection methods, and usually not subject to vaccines, other therapeutics, or readily applied prevention programs. Of the 175 organisms considered to be pathogenic in humans and commonly cited as emerging, 132, or 75% are zoonotic ( Taylor et al., 2001 ). The emergence of new diseases, such as SARS, has been linked to increased contact between humans and the animals carrying the diseases. The spread of H5N1 avian influenza virus in Asia that infected domestic poultry, swine, cats, wild birds (pigeons and crows), and humans is related to changes in agricultural practices of livestock industries. Animals are also carriers of many insect-transmitted pathogens. When the uncertainties associated with transmission from one species to another are added to the ever-increasing mobility of society, the potential interface between those conditions and human food safety, and the heightened concerns about possible effect of bioterrorism on animals (intentional introduction of an animal disease with the intention of causing economic consequences or transmission of disease to humans), the urgency of comprehensive research and implementation becomes obvious.

Veterinary researchers are employed in a number of capacities in EID research. Because of the predominance of zoonotic pathogens in EID outbreaks, veterinarians have been key parts of the teams attempting to identify wildlife reservoirs of hantavirus, Lyme disease, West Nile virus, leptospirosis, Lassa fever, Ebola virus, Nipah virus, Hendra virus, and others. Veterinarians with epidemiological training have been involved in most of the major outbreak investigations undertaken by CDC.

Outbreaks of new zoonotic agents occur almost every year, and they have serious health and economic consequences. For instance, SARS coronavirus, which appears to have wildlife origins, caused over 700 deaths and $50 billion in losses to the global economy in 2003 ( Guan et al., 2003 ; Rota et al., 2003 ). The zoonotic predominance among EIDs suggests a growing need for veterinary researchers to understand dynamics of wildlife pathogens that have emerged or are likely to emerge into human populations (for example, West Nile virus and viruses related to SARS coronavirus or Nipah virus). The ability of these emerging pathogens to spread rapidly across the planet is enhanced by a large and increasing volume of trade in wildlife species that can act as introduction vectors. For example, monkeypox was imported into Wisconsin through the exotic-pet trade industry.

The scope of EID research has been widened to include emerging diseases of marine and terrestrial wildlife and domestic animals ( Anon, 1998 ; Daszak et al., 2000 ; Dobson and Foufopoulos, 2001 ; Harvell et al., 1999 ; Nettles, 1996 ). EIDs are responsible for population declines and mass mortality of wildlife ( Daszak et al., 2000 ), loss of coral reefs and other marine resources globally ( Harvell et al, 1999 ), and threats to global food-animal markets ( NRC, 2002b ; see also section on food-producing animals ).

Veterinary involvement in EID research is critical. For example, BSE was originally discovered by veterinary pathologists, and the dynamics of its spread were understood by veterinary epidemiologists working with mathematical modelers, all before it emerged in the human population. In addition, veterinary institutes and veterinary medical researchers were critical in studying the pathogenesis of the 1918 human pandemic influenza virus in animal models to understand the molecular development and prevention of human influenza pandemics ( Kash et al., 2004 ; Tumpey et al., 2004 ). Understanding how environmental or population changes select for emergence of new zoonotic pathogens from the “zoonotic pool” ( Morse, 1993 ) is a goal discussed in both National Research Council reports on EIDs ( NRC, 2003b ). Useful models are a number of studies funded through the National Institutes of Health/National Science Foundation initiative in ecology of infectious diseases ( NIH, 2002 ) and a recent study of retrovirus emergence in bush meat-hunters in west Africa ( Wolfe et al., 2004 ).

Examples of Critical Research Issues

• A preemptive approach to predict and prevent infectious diseases.
• New tools to identify novel, potentially zoonotic pathogens in wildlife populations that may be the next HIV/AIDS or SARS coronavirus. Such tools will include microarrays and other sophisticated biotechnological applications based on the pool of known zoonotic EIDs that wildlife populations harbor.
• Increased involvement of veterinary researchers in understanding the wildlife trade as a mechanism of EID introduction and in understanding how zoonotic bioterrorism agents may behave if released in the United States.
• The causes, anthropogenic, ecological and environmental drivers, and effects of emerging diseases of livestock and wildlife.

Veterinary research in EID would reduce human mortality due to new emerging diseases, help to prevent future outbreaks of unknown diseases, and help to prevent or deter the introduction and dissemination of pathogens into the United States. This research will also have important economic benefits in reducing public-health costs and disruption of trade and industry.

Ecosystem Health

The field of ecosystem health developed in Canada with the formation of the International Society for Ecosystem Health in 1994 and the launch of its journal Ecosystem Health (superseded by Ecohealth ). The field approaches health as a metaphor in that a healthy ecosystem is one with the full assemblage of species, each with healthy populations. Research in ecosystem health allows a more complete understanding of how disease organisms, toxicants, and health issues affect animal and human populations and the functioning of ecosystems. Ultimately, breakdown of ecosystem health leads to loss of ecosystem functions and affects human health and welfare through effects on agriculture, hunting, fishing and livestock production, and food animal safety.

Veterinary researchers have an important role to play in the advancement of ecosystem health and can contribute in numerous and diverse ways. For example, veterinary researchers have been involved in the characterization of a multispecies (human, companion animal, and marine mammal) outbreak of cryptococcosis ( Stephen et al., 2002 ), in identifying indicators of ecosystem health ( Stephen and Ribble, 2001 ), and in using ecosystem-health concepts for wildlife conservation (for example, Wildlife Conservation Society Field Veterinary Program). Understanding the effects or ecological footprint of terrestrial and aquatic animal agriculture on ecosystems and social systems and how sustainable practices can be developed is critical in both developed and developing nations ( Tilman et al, 2003 ).

• Definition of what constitutes a healthy ecosystem.
• Development of reliable and predictive indicators of ecosystem health.
• Characterization of the complex interaction between humans, domestic and wild animals, and the environment to predict risks to the health of these populations.
• Studies of the interaction between human and animal communities by multidisciplinary teams that include zoo veterinarians, ecologists and toxicologists, and public-policy experts to understand how human activities affect ecosystems and all their inhabitants, including humans.

Failure to address research in ecosystem health would lead to substantial and unpredictable risks (such as infectious disease, food safety, water-borne illness, toxins) to the health of humans and domestic and wild animals. Biodiversity in wild animal and plant populations would be at risk as a result of unhealthy and unsustainable ecosystems.

Social Policies, Societal Needs, and Expectations Including Animal Welfare

The care and use of research animals are governed by USDA regulations and PHS policy, which were implemented to address societal concerns about laboratory animal welfare. These regulations and standards include requirements for the oversight of animal research by Institutional Animal Care and Use Committees and standards for laboratory animal husbandry, housing and enrichment, environmental conditions, and veterinary medical care. However, some of the standards are not supported by scientific analysis. In particular, studies that objectively define, measure, and validate the benefits of social housing and environmental enrichment are inadequate. In the absence of scientific studies that support animal care standards, arbitrary guidelines can lead to inappropriate care, cause undesirable changes in an animal's physiological or behavioral status, produce confounding research results, and unnecessarily increase the cost of animal research. It is imperative that the guidelines and recommendations be strongly supported by scientific study. (See subsection on Laboratory Animals under the Animal Health section.)

Although government standards have been established for laboratory animals, the management of food-producing animals is based largely on practices developed and implemented by animal scientists and food-animal producers. The science-based, objective literature on the impact of physical environment—such as space requirements and the impact of confinement or group housing—on food-producing animals is far from adequate and represents a major and critical area for future comprehensive research ( Mench, 1992 ; Fraser, 2003 ). Pressure from major users of animal food products (such as fast-food chains) is expected to advance the urgency of the need, but the expertise needed to achieve the needed results is lacking in the scientific community, although excellent progress is being made in animal handling and transportation, livestock behavior and facility design, and humane slaughter practices ( Grandin, 2000 ; see also http://www.grandin.com/ ). In addition, research is being conducted in Europe, notably the Netherlands, on the physical environments of pigs and poultry. Some common management practices—such as veal calf production; sow gestation crates; beak-dubbing and comb removal in chickens; and dehorning, castration, and branding of cattle—were developed to improve production or prevent injuries to other animals and humans, but have also raised public concerns about animal welfare. In addition, the effects of new products and technologies used to enhance animal production, including growth hormones and genetic modification, have caused some public concerns.

In addition to laboratory animals and food-producing animals, welfare is an important consideration for animals used for entertainment, racing, hunting, military and police activities, pet therapy, service (such as Eye Seeing dogs), recreation, and companionship. Science-based methods for measuring stress and distress and stress-related effects in animals are essential if substantial progress is to be made in ensuring the welfare of various species. Such efforts require complex multifaceted studies involving expertise in veterinary medicine, animal science, animal behavior, endocrinology, neurology, and pharmacology.

Scientifically based studies can and should be used to make sound public policy and to set responsible regulatory standards. For example, research data have demonstrated that commercially available rabies vaccines will protect dogs for at least 3 years and are therefore federally licensed for a 3-year duration. However, some individual states and counties have established regulations that require more frequent vaccination, despite research evidence that demonstrates the potential adverse effects of such practices. Rabies vaccination can produce tumors in dogs and cats at the injection site, cause serious neurological and immunological adverse effects and death in any species, and induce autoimmune thyroiditis in dogs ( Paul et al., 2003 ; Schultz, 1999 ; Scott-Moncrieff et al., 2002 ; Vascellari et al., 2003 ). Guidelines for canine and feline vaccination have also been developed by the AVMA Council on Biologic and Therapeutic Agents and AAHA task force the American Association of Feline Practitioners and the Academy of Feline Medicine Advisory Panel on Feline Vaccines, on the basis of evidence from veterinary research and published studies ( Elston et al., 1998 ; Klingborg, 2002; Paul et al., 2003 ).

Risk analysis is an important public-policy framework being used both nationally and internationally to make regulatory decisions regarding food safety and to formulate animal trade policies. Science-based risk assessments on the relation of specific pathogens or toxicants to animal and human health are a critical component of the risk analysis. Findings derived from research to identify and characterize hazards and assess exposures are the bases of modeling risk assessments. Sound risk assessments will require a wide array of research on the hazards of and exposure to diseases.

Valid scientific studies should also help to determine the outcome of legal decisions, which may otherwise be driven by emotionally based considerations. During the last decade, for example, several municipalities have adopted the term guardian instead of pet owner. Such changes may eventually lead to court challenges regarding the legal standing of animals and how they are used by society. In addition, many law schools have established centers that specialize in animal “rights”. Again, scientific evidence will be important to validate or refute legal challenges with respect to animals.

• Studies that objectively define, measure, and validate the benefits of social housing and environment enrichment.
• Science-based methods to measure stress and distress and stress-related effects in animals.
• Scientific analysis that uses quantifiable indicators to measure the effects of pharmaceutical agents and genetic modifications on animal welfare.
• Multidisciplinary studies of detection, control and prevention of large-scale zoonotic disease outbreaks that require disposal of large numbers of animals.

Additional research is required to determine the optimal care and use of animals and to support the development of sound public policies governing animal welfare. Research is also required to ensure best management practices of animals in the face of a widespread disaster involving animals and to protect human health.

Exotic and Caged Pets

Exotic and caged pets typically include birds, small mammals (such as ferrets, rabbits, hamsters, guinea pigs, and gerbils), reptiles (such as turtles, lizards, and snakes), and amphibians (such as frogs). Exotic-pet trade is a growing industry that is estimated to be worth more than $10 billion ( Kuehn, 2004a ). In the case of most species—such as reptiles, amphibians, marsupials, exotic birds, and mammals—little research has been done on their behavioral and husbandry needs. Many medical problems in exotic pets are related to poor husbandry ( Kuehn, 2004b ). In addition, limited information is available on the treatment of their diseases. Although the volume of information available on exotic and caged pets has increased considerably over the last few decades, most of it is anecdotal or derived from case reports, because most veterinarians involved with exotic pets provide clinical services and are not actively engaged in research.

The recent outbreak of monkeypox transmitted by prairie dogs that were housed or transported with African rodents from Ghana and the resurgence of salmonellosis contracted from reptiles (iguanas and turtles), marsupials (sugar gliders), and small mammals (hedgehogs) readily illustrate the potential risk that exotic-pet ownership poses ( Check, 2004 ; Gross, 2003 ; Woodward et al., 1997 ). There has been considerable growth in the demand for and ownership of exotic and caged pets ( Doolen, 1996 ; http://epw.senate.gov/hearing_statements.cfm?id2=212880 ). The demand is putting increasing pressure on veterinarians who treat exotic and caged animals to keep up with the highly species-specific needs of their patients.

• Characterization of the zoonotic pathogens capable of being carried by exotic species and also those pathogens that may be transmitted to domestic and wild animal populations.
• Improved methods of diagnosis and treatment of exotic animal diseases, especially in regards to safe and effective anesthetic and analgesic protocols.
• Determination of appropriate husbandry requirements for many exotic species.

Given the increasing number and diversity of exotic pets, veterinary research is necessary to identify important infectious diseases that may pose a risk of transmission to humans and domestic and wild animals. Research on the behavioral, husbandry, and medical needs of exotic pets is also necessary to enhance their quality of life and to contribute to the comparative understanding of diseases in other species.

This chapter illustrates that veterinary research is a diverse enterprise that involves many disciplines and species and has a substantial effect on human health and the economy. In many fields, veterinary research is about characterizing the health implications of changing relationships and the boundaries between species and their environments. The compelling but difficult question is, What is the most important? Although research priorities have been outlined in each area, the different areas of veterinary research were not prioritized against each other. Clearly, issues related to homeland security (such as biosecurity) and food safety stand out because of the potential for catastrophic effects on human and animal health. However, problems often arise from fields that have been overlooked (for example, exotic pets) and many important advances come from fields that may not be recognized by some as priorities so that a balanced approach to support research in the above areas must be sought. The key question regarding research priorities is not what topic should be investigated first, but how a strong and flexible national capacity for veterinary research can be built and maintained to maximize the contribution of veterinary research to the health and welfare of animals and people.

• Cite this Page National Research Council (US) Committee on the National Needs for Research in Veterinary Science . Critical Needs for Research in Veterinary Science. Washington (DC): National Academies Press (US); 2005. 2, Progress and Opportunities in Veterinary Research.
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