how to write an essay for scientific method

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Biology library

Course: biology library   >   unit 1, the scientific method.

• Controlled experiments
• The scientific method and experimental design

Introduction

• Make an observation.
• Ask a question.
• Form a hypothesis , or testable explanation.
• Make a prediction based on the hypothesis.
• Test the prediction.
• Iterate: use the results to make new hypotheses or predictions.

Scientific method example: Failure to toast

1. make an observation..

• Observation: the toaster won't toast.

2. Ask a question.

• Question: Why won't my toaster toast?

3. Propose a hypothesis.

• Hypothesis: Maybe the outlet is broken.

4. Make predictions.

• Prediction: If I plug the toaster into a different outlet, then it will toast the bread.

5. Test the predictions.

• Test of prediction: Plug the toaster into a different outlet and try again.
• If the toaster does toast, then the hypothesis is supported—likely correct.
• If the toaster doesn't toast, then the hypothesis is not supported—likely wrong.

Logical possibility

Practical possibility, building a body of evidence, 6. iterate..

• Iteration time!
• If the hypothesis was supported, we might do additional tests to confirm it, or revise it to be more specific. For instance, we might investigate why the outlet is broken.
• If the hypothesis was not supported, we would come up with a new hypothesis. For instance, the next hypothesis might be that there's a broken wire in the toaster.

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Scientific Method: Role and Importance Essay

The scientific method is a problem-solving strategy that is at the heart of biology and other sciences. There are five steps included in the scientific method that is making an observation, asking a question, forming a hypothesis or an explanation that could be tested, and predicting the test. After that, in the feedback step that is iterating, the results are used to make new predictions. The scientific method is almost always an iterative process. In other words, rather than a straight line, it is a cycle. The outcome of one round of questioning generates feedback that helps to enhance the next round of questioning.

Science is an activity that involves the logical explanation, prediction, and control of empirical phenomena. The concepts of reasoning applicable to the pursuit of this endeavor are referred to as scientific reasoning (Cowles, 2020). They include topics such as experimental design, hypothesis testing, and data interpretation. All sciences, including social sciences, follow the scientific method (Cowles, 2020). Different questions and tests are asked and performed by scientists in various domains. They do, however, have a common approach to finding logical and evidence-based answers.

Scientific reasoning is fundamental for all types of scientific study, not simply institutional research. Scientists do employ specific ideas that non-scientists do not have to use in everyday life. However, many reasoning principles are useful in everyday life. Even if one is not a scientist, they must use excellent reasoning to understand, anticipate, and regulate the events that occur in the environment. When one wants to start their careers, preserve their finances, or enhance their health, they need to acquire evidence to determine the most effective method for achieving our goals. Good scientific thinking skills come in handy in all of these situations.

Experiments, surveys, case studies, descriptive studies, and non-descriptive studies are all forms of research used in the scientific method. In an experiment, a researcher manipulates certain factors in a controlled environment and assesses their impact on other variables (Black, 2018). Descriptive research focuses on the nature of the relationship between the variables being studied rather than on cause and effect. A case study is a detailed examination of a single instance in which something unexpected has occurred. This is normally done with a single individual in extreme or exceptional instances. Large groups of individuals are polled to answer questions about certain topics in surveys. Correlational approaches are used in non-descriptive investigations to anticipate the link between two or more variables.

The Lau and Chan technique describes how to assess the validity of a theory or hypothesis using the scientific method, also known as the hypothetical-deductive method (Lau & Chan, 2017). For testing theories or hypotheses, the hypothetical-deductive technique (HD method) is highly useful. It is sometimes referred to as “scientific procedure.” This is not quite right because science can’t possibly employ only one approach. However, the HD technique is critical since it is one of the most fundamental approaches used in many scientific disciplines, including economics, physics, and biochemistry. Its implementation can be broken down into four stages. The stages include using the hypothetical-deductive method, identifying the testable hypothesis, generating the predictions according to the hypothesis, and using experiments in order to check the predictions (Cowles, 2020). If the predictions that are tested turn out to be correct, the hypothesis will be confirmed. Suppose the results are incorrect; the hypothesis would be disconfirmed.

The HD method instructs us on how to test a hypothesis, and each scientific theory must be testable.

One cannot discover evidence to illustrate whether a theory is likely or not if it cannot be tested. It cannot be considered scientific information in that circumstance. Consider the possibility that there are ghosts that people cannot see, cannot communicate with, and cannot be detected directly or indirectly. This hypothesis is defined in such a way that testing is not possible. It could still be real, and there could be such ghosts, but people would never know; thus, this cannot be considered a scientific hypothesis. In general, validating a theory’s predictions raises the likelihood that it is right. However, this does not establish definitively that the theory is right in and of itself. When given additional assumptions, a hypothesis frequently creates a prediction. When a forecast fails in this way, the theory may still be valid.

When a theory makes a faulty prediction, it might be difficult to determine whether the theory should be rejected or whether the auxiliary assumptions are flawed. Astronomers in the 19th century, for example, discovered that Newtonian physics could not adequately explain the orbit of the planet Mercury. This is due to the fact that Newtonian physics is incorrect, and you require relativity to get a more accurate orbit prediction. When astronomers discovered Uranus in 1781, they discovered that its orbit did not match Newtonian physics predictions. However, astronomers concluded that it could be explained if Uranus was being affected by another planet, and Neptune was discovered as a result.

I had several instances where I have made assumptions on an important issue regardless of evidence. Once I have prepared the work on the topic of power distribution in the workplace and its relation to gender, I have assumed that possibly because of the general feminine traits, women are less likely to create a strong image of power in comparison with men. In fact, such a hypothesis needs to be tested, and it is testable. For example, I could first define what is meant by feminine traits by collecting data from different biological and psychological sources. After that, I could observe the information regarding what factors or behavior patterns contribute to establishing power in the workplace. If I found the correlation between the feminine character traits, communication style, and behavioral patterns with the distribution of power in the workplace, then I could confirm my hypothesis.

Thus, applying the scientific method can help to improve critical reasoning by using tools from scientific reasoning. By supporting the provided hypothesis with evidence from scientific research and statistical data, one can make their claim more valuable and objective. The scientific method is essential for the creation of scientific theories that explain information and ideas in a scientifically rational manner. In a typical scientific method application, a researcher makes a hypothesis, tests it using various methods, and then alters it based on the results of the tests and experiments. The new hypothesis is then retested, further changed, and retested until it matches observable events and testing results. Hypotheses serve as tools for scientists to collect data in this way. Scientists can build broad general explanations, or scientific theories, based on that evidence and the numerous scientific experiments conducted to investigate possibilities. In conclusion, a scientific method is an important approach to examining the hypothesis. By using the tools of the scientific method, the inferences become rational and objective.

Black, M. (2018). Critical thinking: An introduction to logic and scientific method . Pickle Partners Publishing.

Cowles, H. M. (2020). The Scientific Method . Harvard University Press.

Lau, J., & Chan, J. (2017). Scientific methodology: Tutorials 1-9 .

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IvyPanda. (2023, March 14). Scientific Method: Role and Importance. https://ivypanda.com/essays/scientific-method-role-and-importance/

"Scientific Method: Role and Importance." IvyPanda , 14 Mar. 2023, ivypanda.com/essays/scientific-method-role-and-importance/.

IvyPanda . (2023) 'Scientific Method: Role and Importance'. 14 March.

IvyPanda . 2023. "Scientific Method: Role and Importance." March 14, 2023. https://ivypanda.com/essays/scientific-method-role-and-importance/.

1. IvyPanda . "Scientific Method: Role and Importance." March 14, 2023. https://ivypanda.com/essays/scientific-method-role-and-importance/.

Bibliography

IvyPanda . "Scientific Method: Role and Importance." March 14, 2023. https://ivypanda.com/essays/scientific-method-role-and-importance/.

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Writing a scientific paper.

• Writing a lab report
• INTRODUCTION

Writing a "good" methods section

"methods checklist" from: how to write a good scientific paper. chris a. mack. spie. 2018..

• LITERATURE CITED
• Bibliography of guides to scientific writing and presenting
• Peer Review
• Presentations
• Lab Report Writing Guides on the Web

The purpose is to provide enough detail that a competent worker could repeat the experiment. Many of your readers will skip this section because they already know from the Introduction the general methods you used. However careful writing of this section is important because for your results to be of scientific merit they must be reproducible. Otherwise your paper does not represent good science.

• Exact technical specifications and quantities and source or method of preparation
• Describe equipment used and provide illustrations where relevant.
• Chronological presentation (but related methods described together)
• Questions about "how" and "how much" are answered for the reader and not left for them to puzzle over
• Discuss statistical methods only if unusual or advanced
• When a large number of components are used prepare tables for the benefit of the reader
• Do not state the action without stating the agent of the action
• Describe how the results were generated with sufficient detail so that an independent researcher (working in the same field) could reproduce the results sufficiently to allow validation of the conclusions.
• Can the reader assess internal validity (conclusions are supported by the results presented)?
• Can the reader assess external validity (conclusions are properly generalized beyond these specific results)?
• Has the chosen method been justified?
• Are data analysis and statistical approaches justified, with assumptions and biases considered?
• Avoid: including results in the Method section; including extraneous details (unnecessary to enable reproducibility or judge validity); treating the method as a chronological history of events; unneeded references to commercial products; references to “proprietary” products or processes unavailable to the reader. 
• << Previous: INTRODUCTION
• Next: RESULTS >>
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Writing the Scientific Paper

When you write about scientific topics to specialists in a particular scientific field, we call that scientific writing. (When you write to non-specialists about scientific topics, we call that science writing.)

The scientific paper has developed over the past three centuries into a tool to communicate the results of scientific inquiry. The main audience for scientific papers is extremely specialized. The purpose of these papers is twofold: to present information so that it is easy to retrieve, and to present enough information that the reader can duplicate the scientific study. A standard format with six main part helps readers to find expected information and analysis:

• Title--subject and what aspect of the subject was studied.
• Abstract--summary of paper: The main reason for the study, the primary results, the main conclusions
• Introduction-- why the study was undertaken
• Methods and Materials-- how the study was undertaken
• Results-- what was found
• Discussion-- why these results could be significant (what the reasons might be for the patterns found or not found)

There are many ways to approach the writing of a scientific paper, and no one way is right. Many people, however, find that drafting chunks in this order works best: Results, Discussion, Introduction, Materials & Methods, Abstract, and, finally, Title.

The title should be very limited and specific. Really, it should be a pithy summary of the article's main focus.

• "Renal disease susceptibility and hypertension are under independent genetic control in the fawn hooded rat"
• "Territory size in Lincoln's Sparrows ( Melospiza lincolnii )"
• "Replacement of deciduous first premolars and dental eruption in archaeocete whales"
• "The Radio-Frequency Single-Electron Transistor (RF-SET): A Fast and Ultrasensitive Electrometer"

This is a summary of your article. Generally between 50-100 words, it should state the goals, results, and the main conclusions of your study. You should list the parameters of your study (when and where was it conducted, if applicable; your sample size; the specific species, proteins, genes, etc., studied). Think of the process of writing the abstract as taking one or two sentences from each of your sections (an introductory sentence, a sentence stating the specific question addressed, a sentence listing your main techniques or procedures, two or three sentences describing your results, and one sentence describing your main conclusion).

Example One

Hypertension, diabetes and hyperlipidemia are risk factors for life-threatening complications such as end-stage renal disease, coronary artery disease and stroke. Why some patients develop complications is unclear, but only susceptibility genes may be involved. To test this notion, we studied crosses involving the fawn-hooded rat, an animal model of hypertension that develops chronic renal failure. Here, we report the localization of two genes, Rf-1 and Rf-2 , responsible for about half of the genetic variation in key indices of renal impairment. In addition, we localize a gene, Bpfh-1 , responsible for about 26% of the genetic variation in blood pressure. Rf-1 strongly affects the risk of renal impairment, but has no significant effect on blood pressure. Our results show that susceptibility to a complication of hypertension is under at least partially independent genetic control from susceptibility to hypertension itself.

Brown, Donna M, A.P. Provoost, M.J. Daly, E.S. Lander, & H.J. Jacob. 1996. "Renal disease susceptibility and hypertension are under indpendent genetic control in the faun-hooded rat." Nature Genetics , 12(1):44-51.

Example Two

We studied survival of 220 calves of radiocollared moose ( Alces alces ) from parturition to the end of July in southcentral Alaska from 1994 to 1997. Prior studies established that predation by brown bears ( Ursus arctos ) was the primary cause of mortality of moose calves in the region. Our objectives were to characterize vulnerability of moose calves to predation as influenced by age, date, snow depths, and previous reproductive success of the mother. We also tested the hypothesis that survival of twin moose calves was independent and identical to that of single calves. Survival of moose calves from parturition through July was 0.27 ± 0.03 SE, and their daily rate of mortality declined at a near constant rate with age in that period. Mean annual survival was 0.22 ± 0.03 SE. Previous winter's snow depths or survival of the mother's previous calf was not related to neonatal survival. Selection for early parturition was evidenced in the 4 years of study by a 6.3% increase in the hazard of death with each daily increase in parturition date. Although there was no significant difference in survival of twin and single moose calves, most twins that died disappeared together during the first 15 days after birth and independently thereafter, suggesting that predators usually killed both when encountered up to that age.

Key words: Alaska, Alces alces , calf survival, moose, Nelchina, parturition synchrony, predation

Testa, J.W., E.F. Becker, & G.R. Lee. 2000. "Temporal patterns in the survival of twin and single moose ( alces alces ) calves in southcentral Alaska." Journal of Mammalogy , 81(1):162-168.

Example Three

We monitored breeding phenology and population levels of Rana yavapaiensis by use of repeated egg mass censuses and visual encounter surveys at Agua Caliente Canyon near Tucson, Arizona, from 1994 to 1996. Adult counts fluctuated erratically within each year of the study but annual means remained similar. Juvenile counts peaked during the fall recruitment season and fell to near zero by early spring. Rana yavapaiensis deposited eggs in two distinct annual episodes, one in spring (March-May) and a much smaller one in fall (September-October). Larvae from the spring deposition period completed metamorphosis in earlv summer. Over the two years of study, 96.6% of egg masses successfully produced larvae. Egg masses were deposited during periods of predictable, moderate stream flow, but not during seasonal periods when flash flooding or drought were likely to affect eggs or larvae. Breeding phenology of Rana yavapaiensis is particularly well suited for life in desert streams with natural flow regimes which include frequent flash flooding and drought at predictable times. The exotic predators of R. yavapaiensis are less able to cope with fluctuating conditions. Unaltered stream flow regimes that allow natural fluctuations in stream discharge may provide refugia for this declining ranid frog from exotic predators by excluding those exotic species that are unable to cope with brief flash flooding and habitat drying.

Sartorius, Shawn S., and Philip C. Rosen. 2000. "Breeding phenology of the lowland leopard frog ( Rana yavepaiensis )." Southwestern Naturalist , 45(3): 267-273.

Introduction

The introduction is where you sketch out the background of your study, including why you have investigated the question that you have and how it relates to earlier research that has been done in the field. It may help to think of an introduction as a telescoping focus, where you begin with the broader context and gradually narrow to the specific problem addressed by the report. A typical (and very useful) construction of an introduction proceeds as follows:

"Echimyid rodents of the genus Proechimys (spiny rats) often are the most abundant and widespread lowland forest rodents throughout much of their range in the Neotropics (Eisenberg 1989). Recent studies suggested that these rodents play an important role in forest dynamics through their activities as seed predators and dispersers of seeds (Adler and Kestrell 1998; Asquith et al 1997; Forget 1991; Hoch and Adler 1997)." (Lambert and Adler, p. 70)

"Our laboratory has been involved in the analysis of the HLA class II genes and their association with autoimmune disorders such as insulin-dependent diabetes mellitus. As part of this work, the laboratory handles a large number of blood samples. In an effort to minimize the expense and urgency of transportation of frozen or liquid blood samples, we have designed a protocol that will preserve the integrity of lymphocyte DNA and enable the transport and storage of samples at ambient temperatures." (Torrance, MacLeod & Hache, p. 64)

"Despite the ubiquity and abundance of P. semispinosus , only two previous studies have assessed habitat use, with both showing a generalized habitat use. [brief summary of these studies]." (Lambert and Adler, p. 70)

"Although very good results have been obtained using polymerase chain reaction (PCR) amplification of DNA extracted from dried blood spots on filter paper (1,4,5,8,9), this preservation method yields limited amounts of DNA and is susceptible to contamination." (Torrance, MacLeod & Hache, p. 64)

"No attempt has been made to quantitatively describe microhabitat characteristics with which this species may be associated. Thus, specific structural features of secondary forests that may promote abundance of spiny rats remains unknown. Such information is essential to understand the role of spiny rats in Neotropical forests, particularly with regard to forest regeneration via interactions with seeds." (Lambert and Adler, p. 71)

"As an alternative, we have been investigating the use of lyophilization ("freeze-drying") of whole blood as a method to preserve sufficient amounts of genomic DNA to perform PCR and Southern Blot analysis." (Torrance, MacLeod & Hache, p. 64)

"We present an analysis of microhabitat use by P. semispinosus in tropical moist forests in central Panama." (Lambert and Adler, p. 71)

"In this report, we summarize our analysis of genomic DNA extracted from lyophilized whole blood." (Torrance, MacLeod & Hache, p. 64)

Methods and Materials

In this section you describe how you performed your study. You need to provide enough information here for the reader to duplicate your experiment. However, be reasonable about who the reader is. Assume that he or she is someone familiar with the basic practices of your field.

It's helpful to both writer and reader to organize this section chronologically: that is, describe each procedure in the order it was performed. For example, DNA-extraction, purification, amplification, assay, detection. Or, study area, study population, sampling technique, variables studied, analysis method.

Include in this section:

• study design: procedures should be listed and described, or the reader should be referred to papers that have already described the used procedure
• particular techniques used and why, if relevant
• modifications of any techniques; be sure to describe the modification
• specialized equipment, including brand-names
• temporal, spatial, and historical description of study area and studied population
• assumptions underlying the study
• statistical methods, including software programs

Example description of activity

Chromosomal DNA was denatured for the first cycle by incubating the slides in 70% deionized formamide; 2x standard saline citrate (SSC) at 70ºC for 2 min, followed by 70% ethanol at -20ºC and then 90% and 100% ethanol at room temperature, followed by air drying. (Rouwendal et al ., p. 79)

Example description of assumptions

We considered seeds left in the petri dish to be unharvested and those scattered singly on the surface of a tile to be scattered and also unharvested. We considered seeds in cheek pouches to be harvested but not cached, those stored in the nestbox to be larderhoarded, and those buried in caching sites within the arena to be scatterhoarded. (Krupa and Geluso, p. 99)

Examples of use of specialized equipment

• Oligonucleotide primers were prepared using the Applied Biosystems Model 318A (Foster City, CA) DNA Synthesizer according to the manufacturers' instructions. (Rouwendal et al ., p.78)
• We first visually reviewed the complete song sample of an individual using spectrograms produced on a Princeton Applied Research Real Time Spectrum Analyzer (model 4512). (Peters et al ., p. 937)

Example of use of a certain technique

Frogs were monitored using visual encounter transects (Crump and Scott, 1994). (Sartorius and Rosen, p. 269)

Example description of statistical analysis

We used Wilcox rank-sum tests for all comparisons of pre-experimental scores and for all comparisons of hue, saturation, and brightness scores between various groups of birds ... All P -values are two-tailed unless otherwise noted. (Brawner et al ., p. 955)

This section presents the facts--what was found in the course of this investigation. Detailed data--measurements, counts, percentages, patterns--usually appear in tables, figures, and graphs, and the text of the section draws attention to the key data and relationships among data. Three rules of thumb will help you with this section:

• present results clearly and logically
• avoid excess verbiage
• consider providing a one-sentence summary at the beginning of each paragraph if you think it will help your reader understand your data

Remember to use table and figures effectively. But don't expect these to stand alone.

Some examples of well-organized and easy-to-follow results:

• Size of the aquatic habitat at Agua Caliente Canyon varied dramatically throughout the year. The site contained three rockbound tinajas (bedrock pools) that did not dry during this study. During periods of high stream discharge seven more seasonal pools and intermittent stretches of riffle became available. Perennial and seasonal pool levels remained stable from late February through early May. Between mid-May and mid-July seasonal pools dried until they disappeared. Perennial pools shrank in surface area from a range of 30-60 m² to 3-5- M². (Sartorius and Rosen, Sept. 2000: 269)

Notice how the second sample points out what is important in the accompanying figure. It makes us aware of relationships that we may not have noticed quickly otherwise and that will be important to the discussion.

A similar test result is obtained with a primer derived from the human ß-satellite... This primer (AGTGCAGAGATATGTCACAATG-CCCC: Oligo 435) labels 6 sites in the PRINS reaction: the chromosomes 1, one pair of acrocentrics and, more weakly, the chromosomes 9 (Fig. 2a). After 10 cycles of PCR-IS, the number of sites labeled has doubled (Fig. 2b); after 20 cycles, the number of sites labeled is the same but the signals are stronger (Fig. 2c) (Rouwendal et al ., July 93:80).

Related Information: Use Tables and Figures Effectively

Do not repeat all of the information in the text that appears in a table, but do summarize it. For example, if you present a table of temperature measurements taken at various times, describe the general pattern of temperature change and refer to the table.

"The temperature of the solution increased rapidly at first, going from 50º to 80º in the first three minutes (Table 1)."

You don't want to list every single measurement in the text ("After one minute, the temperature had risen to 55º. After two minutes, it had risen to 58º," etc.). There is no hard and fast rule about when to report all measurements in the text and when to put the measurements in a table and refer to them, but use your common sense. Remember that readers have all that data in the accompanying tables and figures, so your task in this section is to highlight key data, changes, or relationships.

In this section you discuss your results. What aspect you choose to focus on depends on your results and on the main questions addressed by them. For example, if you were testing a new technique, you will want to discuss how useful this technique is: how well did it work, what are the benefits and drawbacks, etc. If you are presenting data that appear to refute or support earlier research, you will want to analyze both your own data and the earlier data--what conditions are different? how much difference is due to a change in the study design, and how much to a new property in the study subject? You may discuss the implication of your research--particularly if it has a direct bearing on a practical issue, such as conservation or public health.

This section centers on speculation . However, this does not free you to present wild and haphazard guesses. Focus your discussion around a particular question or hypothesis. Use subheadings to organize your thoughts, if necessary.

This section depends on a logical organization so readers can see the connection between your study question and your results. One typical approach is to make a list of all the ideas that you will discuss and to work out the logical relationships between them--what idea is most important? or, what point is most clearly made by your data? what ideas are subordinate to the main idea? what are the connections between ideas?

Achieving the Scientific Voice

Eight tips will help you match your style for most scientific publications.

• Develop a precise vocabulary: read the literature to become fluent, or at least familiar with, the sort of language that is standard to describe what you're trying to describe.
• Once you've labeled an activity, a condition, or a period of time, use that label consistently throughout the paper. Consistency is more important than creativity.
• Define your terms and your assumptions.
• Include all the information the reader needs to interpret your data.
• Remember, the key to all scientific discourse is that it be reproducible . Have you presented enough information clearly enough that the reader could reproduce your experiment, your research, or your investigation?
• When describing an activity, break it down into elements that can be described and labeled, and then present them in the order they occurred.
• When you use numbers, use them effectively. Don't present them so that they cause more work for the reader.
• Include details before conclusions, but only include those details you have been able to observe by the methods you have described. Do not include your feelings, attitudes, impressions, or opinions.
• Research your format and citations: do these match what have been used in current relevant journals?
• Run a spellcheck and proofread carefully. Read your paper out loud, and/ or have a friend look over it for misspelled words, missing words, etc.

Applying the Principles, Example 1

The following example needs more precise information. Look at the original and revised paragraphs to see how revising with these guidelines in mind can make the text clearer and more informative:

Before: Each male sang a definite number of songs while singing. They start with a whistle and then go from there. Each new song is always different, but made up an overall repertoire that was completed before starting over again. In 16 cases (84%), no new songs were sung after the first 20, even though we counted about 44 songs for each bird.

After: Each male used a discrete number of song types in his singing. Each song began with an introductory whistle, followed by a distinctive, complex series of fluty warbles (Fig. 1). Successive songs were always different, and five of the 19 males presented their entire song repertoire before repeating any of their song types (i.e., the first IO recorded songs revealed the entire repertoire of 10 song types). Each song type recurred in long sequences of singing, so that we could be confident that we had recorded the entire repertoire of commonly used songs by each male. For 16 of the 19 males, no new song types were encountered after the first 20 songs, even though we analyzed and average of 44 songs/male (range 30-59).

Applying the Principles, Example 2

In this set of examples, even a few changes in wording result in a more precise second version. Look at the original and revised paragraphs to see how revising with these guidelines in mind can make the text clearer and more informative:

Before: The study area was on Mt. Cain and Maquilla Peak in British Columbia, Canada. The study area is about 12,000 ha of coastal montane forest. The area is both managed and unmanaged and ranges from 600-1650m. The most common trees present are mountain hemlock ( Tsuga mertensiana ), western hemlock ( Tsuga heterophylla ), yellow cedar ( Chamaecyparis nootkatensis ), and amabilis fir ( Abies amabilis ).

After: The study took place on Mt. Cain and Maquilla Peak (50'1 3'N, 126'1 8'W), Vancouver Island, British Columbia. The study area encompassed 11,800 ha of coastal montane forest. The landscape consisted of managed and unmanaged stands of coastal montane forest, 600-1650 m in elevation. The dominant tree species included mountain hemlock ( Tsuga mertensiana ), western hemlock ( Tsuga heterophylla ), yellow cedar ( Chamaecyparis nootkatensis ), and amabilis fir ( Abies amabilis ).

Two Tips for Sentence Clarity

Although you will want to consider more detailed stylistic revisions as you become more comfortable with scientific writing, two tips can get you started:

First, the verb should follow the subject as soon as possible.

Really Hard to Read : "The smallest of the URF's (URFA6L), a 207-nucleotide (nt) reading frame overlapping out of phase the NH2- terminal portion of the adenosinetriphosphatase (ATPase) subunit 6 gene has been identified as the animal equivalent of the recently discovered yeast H+-ATPase subunit gene."

Less Hard to Read : "The smallest of the UR-F's is URFA6L, a 207-nucleotide (nt) reading frame overlapping out of phase the NH2-terminal portion of the adenosinetriphosphatase (ATPase) subunit 6 gene; it has been identified as the animal equivalent of the recently discovered yeast H+-ATPase subunit 8 gene."

Second, place familiar information first in a clause, a sentence, or a paragraph, and put the new and unfamiliar information later.

More confusing : The epidermis, the dermis, and the subcutaneous layer are the three layers of the skin. A layer of dead skin cells makes up the epidermis, which forms the body's shield against the world. Blood vessels, carrying nourishment, and nerve endings, which relay information about the outside world, are found in the dermis. Sweat glands and fat cells make up the third layer, the subcutaneous layer.

Less confusing : The skin consists of three layers: the epidermis, the dermis, and the subcutaneous layer. The epidermis is made up of dead skin cells, and forms a protective shield between the body and the world. The dermis contains the blood vessels and nerve endings that nourish the skin and make it receptive to outside stimuli. The subcutaneous layer contains the sweat glands and fat cells which perform other functions of the skin.

Bibliography

• Scientific Writing for Graduate Students . F. P. Woodford. Bethesda, MD: Council of Biology Editors, 1968. [A manual on the teaching of writing to graduate students--very clear and direct.]
• Scientific Style and Format . Council of Biology Editors. Cambridge: Cambridge University Press, 1994.
• "The science of scientific writing." George Gopen and Judith Swann. The American Scientist , Vol. 78, Nov.-Dec. 1990. Pp 550-558.
• "What's right about scientific writing." Alan Gross and Joseph Harmon. The Scientist , Dec. 6 1999. Pp. 20-21.
• "A Quick Fix for Figure Legends and Table Headings." Donald Kroodsma. The Auk , 117 (4): 1081-1083, 2000.

Wortman-Wunder, Emily, & Kate Kiefer. (1998). Writing the Scientific Paper. Writing@CSU . Colorado State University. https://writing.colostate.edu/resources/writing/guides/.

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For more information on writing your scientific papers, here are some good resources:

http://writingcenter.unc.edu/handouts/scientific-reports/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559667/

The Scientific Method

When writing or reading about science, it is useful to keep the scientific method in mind. The scientific method is used as a model to construct writing that can be shared with others in a logical and informative way. Any piece of scientific writing is informative and persuasive: informative because the author is telling the audience how he or she conducted their research and what new information they learned, and persuasive because science papers demonstrate how that new information was obtained and what conclusions can be drawn from the data collected. The format of most journal articles follows the steps of the scientific method, with Introduction, Methods, and Results sections at a minimum. 

Evidence and Argumentation

Science writing has a persuasive element to it. Researchers need to convince others that they have done their experiments properly and that they have answered their central research questions. Therefore, all science papers, even theoretical ones, make use of evidence to support their points. Remember that statistical measures, while extremely useful, are not the only source of evidence. Observations of even a single event can be useful in the right context. Remember to use logic to link your evidence and claims together!

Tips for writing a persuasive paper:  http://www.facstaff.bucknell.edu/awolaver/term1.htm

Relating evidence and ideas:  http://undsci.berkeley.edu/article/coreofscience_01

Clarity and Reader Expectations

Many people complain that scientific literature is difficult to understand because of the complicated language and the use of jargon. However, scientific literature can be difficult to understand even if one is familiar with the concepts being discussed.

To avoid confusing the reader, writers should focus on writing clearly and keeping the reader’s expectations in mind. In a scientific paper, it is important that most readers will agree with the information being presented. Writing in a clear and concise way helps the writer accomplish this. Use the paper as a story-telling medium. Concentrate on showing the reader that your experiments show definite conclusion, and how this contribution changes the state of knowledge in the field.

Gopen and Swan (1990) offer the following seven easy ways to make your writing more clear and to say what you want the reader to hear.

• Follow a grammatical subject as soon as possible with its verb.
• Place in the stress position the "new information" you want the reader to emphasize. 
• Place the person or thing whose "story" a sentence is telling at the beginning of the sentence, in the topic position. 
• Place appropriate "old information" (material already stated in the discourse) in the topic position for linkage backward and contextualization forward. 
• Articulate the action of every clause or sentence in its verb. 
• In general, provide context for your reader before asking that reader to consider anything new. 
• In general, try to ensure that the relative emphases of the substance coincide with the relative expectations for emphasis raised by the structure.

[Gopen, G. and Swan, J. 1990. The Science of Scientific Writing. American Scientist. Available here: https://www.americanscientist.org/blog/the-long-view/the-science-of-scientific-writing  ]

One major problem many students have when they start writing papers is using so called “running jumps.” This is the placement of unnecessary words at the beginning of a sentence. For example:

RUNNING JUMP: According to the researchers, the control group showed more change in chlorophyll production (Smith et. al., 2014).

NO RUNNING JUMP: The control group showed more change in chlorophyll production (Smith et. al., 2014).

We’ve already cited a study, so it’s clear that we are referring to researchers and their findings. So the first part of the sentence is unnecessary. 

Try to limit the number of ideas expressed in a single sentence. If a sentence seems like it is trying to say more than two things at once, split it into two sentences.  If a sentence is long and tangled and just doesn't make sense, don't try to perform "surgery" to fix the sentence. Instead, "kill" the sentence and start over, using short, direct sentences to express what you mean.

If you can make a noun phrase into a verb, do it! For example, made note of = noted, provided a similar opinion = agreed with, conducted an experiment = experimented, etc.

Avoid the Passive Voice Where You Can

If a sentence is written in the passive voice, the subject of the sentence (person/thing doing the action) does not come first; rather, the object of the sentence (person/thing not doing the action) is the first noun in the sentence. 

PASSIVE: Radiation was the mechanism by which the samples were sterilized.

MORE ACTIVE: The samples were sterilized using radiation.

ACTIVE: We sterilized the samples using radiation.

Professors (and scientific journals) have differing opinions on the use of passive voice. Some consider it unacceptable, but many are more lenient. And in fact, often it will make more sense to write in the passive voice in certain sections (i.e. Methods) and when you can't use first-person pronouns like "I." In any case, reducing overuse of passive voice in your writing makes it more concise and easier to understand.

Here's a useful link for clear scientific writing style: http://www.nature.com/scitable/topicpage/effective-writing-13815989

Posters are often used as an accompaniment to a talk or presentation, or as a substitute. You’ve probably seen posters hanging up around campus, showcasing students' research. The idea of a poster is to simplify a study and present it in a visual way, so it can be understood by a wide audience. The most important thing to remember when designing a poster (or completing any kind of published work) is to follow the guidelines given. If your instructor, or the conference you’re presenting at, wants a certain format, adhere to that format. These three rules are especially important to follow:

• Shorter is better: make sure that your poster does not contain too much text! Packing text onto the poster makes it difficult to read and understand.
• Bigger is better. No, this is not a contradiction of rule 1! Make sure your text is large enough to read, and readable against the background of the poster.
• Use images. The key aspect of a poster is that it is a visual medium. Include graphs, photos, and illustrations of your work.

Here are some excellent tips and templates for research posters: 

1.  http://colinpurrington.com/tips/academic/posterdesign

2. http://www.waspacegrant.org

3.  http://www.personal.psu.edu/drs18/postershow/ : Poster tips from Penn State

Most scientific citation styles are based on APA format. It’s totally okay to use a resource to look up how to format a paper in APA style! As you become more familiar with the format, you will become less reliant on these resources, but for now, here are some sites that may be useful .

Our APA guide

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How to Write a Scientific Paper: Practical Guidelines

Edgard delvin.

1 Centre de recherche, CHU Sainte-Justine

2 Département de Biochimie, Université de Montréal, Montréal, Canada

Tahir S. Pillay

3 Department of Chemical Pathology, Faculty of Health Sciences, University of Pretoria

4 Division of Chemical Pathology, University of Cape Town

5 National Health Laboratory Service, CTshwane Academic Division, Pretoria, South Africa

Anthony Newman

6 Life Sciences Department, Elsevier, Amsterdam, The Netherlands

Precise, accurate and clear writing is essential for communicating in health sciences, as publication is an important component in the university criteria for academic promotion and in obtaining funding to support research. In spite of this, the development of writing skills is a subject infrequently included in the curricula of faculties of medicine and allied health sciences. Therefore clinical investigators require tools to fill this gap. The present paper presents a brief historical background to medical publication and practical guidelines for writing scientific papers for acceptance in good journals.

INTRODUCTION

A scientific paper is the formal lasting record of a research process. It is meant to document research protocols, methods, results and conclusions derived from an initial working hypothesis. The first medical accounts date back to antiquity. Imhotep, Pharaoh of the 3 rd Dynasty, could be considered the founder of ancient Egyptian medicine as he has been credited with being the original author of what is now known as the Edwin Smith Papyrus ( Figure 1 ). The Papyrus, by giving some details on cures and anatomical observations, sets the basis of the examination, diagnosis, treatment, and prognosis of numerous diseases. Closer to the Common Era, in 460 BCE, Hippocrates wrote 70 books on medicine. In 1020, the Golden age of the Muslim Culture, Ibn Sina, known as Avicenna ( Figure 2a ), recorded the Canon of medicine that was to become the most used medical text in Europe and Middle East for almost half a millennium. This was followed in the beginning of the 12 th Century bytheextensivetreatiseofMaimonides( Figure 2b ) (Moses ben Maimon) on Greek and Middle Eastern medicine. Of interest, by the end of the 11 th Century Trotula di Ruggiero, a woman physician, wrote several influential books on women’s ailment. A number of other hallmark treatises also became more accessible, thanks to the introduction of the printing press that allowed standardization of the texts. One example is the De Humani Corporis Fabrica by Vesalius which contains hundreds of illustrations of human dissection. Thomas A Lang provides an excellent concise history of scientific publications [ 1 ]. These were the days when writing and publishing scientific or philosophical works were the privilege of the few and hence there was no or little competition and no recorded peer reviewing system. Times have however changed, and contemporary scientists have to compose with an increasingly harsh competition in attracting editors and publishers attention. As an example, the number of reports and reviews on obesity and diabetes has increased from 400 to close to 4000/year and 50 to 600/year respectively over a period of 20 years ( Figure 3 ). The present article, essentially based on TA Lang’s guide for writing a scientific paper [ 1 ], will summarize the steps involved in the process of writing a scientific report and in increasing the likelihood of its acceptance.

The Edwin Smith Papyrus (≈3000 BCE)

Avicenna and Maimonides

Annual publication load in the field of obesity and diabetes over 20 years.

Reasons for publishing are varied. One may write to achieve a post-graduate degree, to obtain funding for pursuing research or for academic promotion. While all 3 reasons are perfectly legitimate, one must ask whether they are sufficient to be considered by editors, publishers and reviewers. Why then should the scientist write? The main reason is to provide to the scientific community data based on hypotheses that are innovative and thus to advance the understanding in a specific domain. One word of caution however, is that if a set of experiments has not been done or reported, it does not mean that it should be. It may simply reflect a lack of interest in it.

DECIDING ON PUBLISHING AND TARGETING THE JOURNAL

In order to assist with the decision process, pres-ent your work orally first to colleagues in your field who may be more experienced in publishing. This step will help you in gauging whether your work is publishable and in shaping the paper.

Targeting the journal, in which you want to present your data, is also a critical step and should be done before starting to write. One hint is to look for journals that have published similar work to yours, and that aims readers most likely to be interested in your research. This will allow your article to be well read and cited. These journals are also those that you are most likely to read on a regular basis and to cite abundantly. The next step is to decide whether you submit your manuscript to a top-ranking impact factor journal or to a journal of lower prestige. Although it is tempting to test the waters, or to obtain reviewers comments, be realistic about the contribution your work provides and submit to a journal with an appropriate rank.

Do not forget that each rejection delays publication and that the basin of reviewers within your specialty is shallow. Thus repeated submission to different journals could likely result in having your work submitted for review to the same re-viewer.

DECIDING ON THE TYPE OF MANUSCRIPT

There are several types of scientific reports: observational, experimental, methodological, theoretical and review. Observational studies include 1) single-case report, 2) collective case reports on a series of patients having for example common signs and symptoms or being followed-up with similar protocols, 3) cross-sectional, 4) cohort studies, and 5) case-control studies. The latter 3 could be perceived as epidemiological studies as they may help establishing the prevalence of a condition, and identify a defined population with and without a particular condition (disease, injury, surgical complication). Experimental reports deal with research that tests a research hypothesis through an established protocol, and, in the case of health sciences, formulate plausible explanations for changes in biological systems. Methodological reports address for example advances in analytical technology, statistical methods and diagnostic approach. Theoretical reports suggest new working hypotheses and principles that have to be supported or disproved through experimental protocols. The review category can be sub-classified as narrative, systematic and meta-analytic. Narrative reviews are often broad overviews that could be biased as they are based on the personal experience of an expert relying on articles of his or her own choice. Systematic reviews and meta-analyses are based on reproducible procedures and on high quality data. Researchers systematically identify and analyze all data collected in articles that test the same working hypothesis, avoiding selection bias, and report the data in a systematic fashion. They are particularly helpful in asking important questions in the field of healthcare and are often the initial step for innovative research. Rules or guidelines in writing such report must be followed if a quality systematic review is to be published.

For clinical research trials and systematic reviews or meta-analyses, use the Consort Statement (Consolidated Standards Of Reporting Trials) and the PRISMA Statement (Preferred Reporting Items for Systematic reviews and Meta-Analyses) respectively [ 2 , 3 ]. This assures the editors and the reviewers that essential elements of the trials and of the reviews were tackled. It also speeds the peer review process. There are several other Statements that apply to epidemiological studies [ 4 ], non-randomized clinical trials [ 5 ], diagnostic test development ( 6 ) and genetic association studies ( 7 ). The Consortium of Laboratory Medicine Journal Editors has also published guidelines for reporting industry-sponsored laboratory research ( 8 ).

INITIAL STEPS IN THE PROCESS OF WRITING A SCIENTIFIC DOCUMENT

Literature review is the initial and essential step before starting your study and writing the scientific report based on it. In this process use multiple databases, multiple keyword combinations. It will allow you to track the latest development in your field and thus avoid you to find out that someone else has performed the study before you, and hence decrease the originality of your study. Do not forget that high-ranking research journals publish results of enough importance and interest to merit their publication.

Determining the authorship and the order of authorship, an ethical issue, is the second essential step, and is unfortunately often neglected. This step may avoid later conflicts as, despite existing guidelines, it remains a sensitive issue owing to personal biases and the internal politics of institutions. The International Committee of Medical Editors has adopted the following guidelines for the biomedical sciences ( 9 ).

“Authorship credit should be based only on: 1) Substantial contributions to the conception and design, or acquisition of data, or analysis and interpretation of data; 2) Drafting the article or revising it critically for important intellectual content; and 3) Final approval of the version to be published. Conditions 1, 2 and 3 must be all met. Acquisition of funding, the collections of data, or general supervision of the research group, by themselves, do not justify authorship.” ( 9 , 10 )

The order of authorship should reflect the individual contribution to the research and to the publication, from most to least ( 11 ). The first author usually carries out the lead for the project reported. However the last author is often mistakenly perceived as the senior author. This is perpetuated from the European tradition and is discouraged. As there are divergent conventions among journals, the order of authorship order may or may not reflect the individual contributions; with the exception that the first author should be the one most responsible for the work.

WRITING EFFECTIVELY

Effective writing requires that the text helps the readers 1) understand the content and the context, 2) remember what the salient points are, 3) find the information rapidly and, 4) use or apply the information given. These cardinal qualities should be adorned with the precise usage of the language, clarity of the text, inclu-siveness of the information, and conciseness. Effective writing also means that you have to focus on the potential readers’ needs. Readers in science are informed individuals who are not passive, and who will formulate their own opinion of your writing whether or not the meaning is clear. Therefore you need to know who your audience is. The following 4 questions should help you writing a reader-based text, meaning written to meet the information needs of readers [ 12 ].

What do you assume your readers already know? In other words, which terms and concepts can you use without explanation, and which do you have to define?

What do they want to know? Readers in science will read only if they think they will learn something of value.

What do they need to know? Your text must contain all the information necessary for the reader to understand it, even if you think this information id obvious to them.

What do they think they know that is not so? Correcting misconceptions can be an important function of communication, and persuading readers to change their minds can be a challenging task.

WRITING THE SCIENTIFIC PAPER

Babbs and Tacker ’ s advice to write as much of the paper before performing the research project or experimental protocol may, at first sight, seem unexpected and counterintuitive [ 13 ], but in fact it is exactly what is being done when writing a research grant application. It will allow you to define the authorship alluded to before. The following section will briefly review the structure of the different sections of a manuscript and describe their purpose.

Reading the instructions to authors of the Journal you have decided to submit your manuscript is the first important step. They provide you with the specific requirements such as the way of listing the authors, type of abstract, word, figure or table limits and citation style. The Mulford Library of University of Toledo website contains instructions to authors for over 3000 journals ( http://mulford.meduoiho.edu/instr/ ).

The general organization of an article follows the IMRAD format (Introduction, Methods, Results, and Discussion). These may however vary. For instance, in clinical research or epidemiology studies, the methods section will include details on the subjects included, and there will be a statement of the limitation of the study. Although conclusions may not always be part of the structure, we believe that it should, even in methodological reports.

The tile page provides essential information so that the editor, reviewers, and readers will identify the manuscript and the authors at a glance as well as enabling them to classify the field to which the article pertains.

The title page must contain the following:

• The tile of the article – it is an important part of the manuscript as it is the most often read and will induce the interested readers to pursue further. Therefore the title should be precise, accurate, specific and truthful;
• Each author’s given name (it may be the full name or initials) and family name;
• Each author’s affiliation;
• Some journals ask for highest academic degree;
• A running title that is usually limited to a number of characters. It must relate to the full title;
• Key words that will serve for indexing;
• For clinical studies, the trial’s registration number;
• The name of the corresponding author with full contact information.

The abstract is also an important section of your manuscript. Importantly, the abstract is the part of the article that your peers will see when consulting publication databases such as PubMed. It is the advertisement to your work and will strongly influence the editor deciding whether it will be submitted to reviewers or not. It will also help the readers decide to read the full article. Hence it has to be comprehensible on its own. Writing an abstract is challenging. You have to carefully select the content and, while being concise, assure to deliver the essence of your manuscript.

Without going into details, there are 3 types of abstracts: descriptive, informative and structured. The descriptive abstract is particularly used for theoretical, methodological or review articles. It usually consists of a single paragraph of 150 words or less. The informative abstract, the most common one, contains specific information given in the article and, are organized with an introduction (background, objectives), methods, results and discussion with or without conclusion. They usually are 150 to 250 words in length. The structured abstract is in essence an informative abstract with sections labeled with headings. They may also be longer and are limited to 250 to 300 words. Recent technology also allows for graphical or even video abstracts. The latter are interesting in the context of cell biology as they enable the investigator to illustrate ex vivo experiment results (phagocytosis process for example).

Qualities of abstracts:

• Understood without reading the full paper. Shoul dcontain no abbreviations.lf abbreviations are used, they must be defined. This however removes space for more important information;
• Contains information consistent with the full report. Conclusions in the abstract must match those given in the full report;
• Is attractive and contains information needed to decide whether to read the full report.

Introduction

The introduction has 3 main goals: to establish the need and importance of your research, to indicate how you have filled the knowledge gap in your field and to give your readers a hint of what they will learn when reading your paper. To fulfil these goals, a four-part introduction consisting of a background statement, a problem statement, an activity statement and a forecasting statement, is best suited. Poorly defined background information and problem setting are the 2 most common weaknesses encountered in introductions. They stem from the false perception that peer readers know what the issue is and why the study to solve it is necessary. Although not a strict rule, the introduction in clinical science journals should target only references needed to establish the rationale for the study and the research protocol. This differ from more basic science or cell biology journals, for which a longer and elaborate introduction may be justified because the research at hand consists of several approaches each requiring background and justification.

The 4-part introduction consists of:

• A background statement that provides the context and the approach of the research;
• A problem statement that describes the nature, scope and importance of the problem or the knowledge gap;
• An activity statement, that details the research question, sets the hypothesis and actions undertaken for the investigation;
• A forecasting statement telling the readers whattheywillfìndwhen readingyourarticle [ 14 ].

Methods section

This section may be named “Materials and Methods”, “Experimental section” or “Patients and Methods” depending upon the type of journal. Its purpose to allow your readers to provide enough information on the methods used for your research and to judge on their adequacy. Although clinical and “basic” research protocols differ, the principles involved in describing the methods share similar features. Hence, the breadth of what is being studied and how the study can be performed is common to both. What differ are the specific settings. For example, when a study is conducted on humans, you must provide, up front, assurance that it has received the approval of you Institution Ethics Review Board (IRB) and that participants have provided full and informed consent. Similarly when the study involves animals, you must affirm that you have the agreement from your Institutional Animal Care and Use Committee (IACUC). These are too often forgotten, and Journals (most of them) abiding to the rules of the Committee on Publication Ethics (COPE) and World Association of Medical Editors (WAME) will require such statement. Although journals publishing research reports in more fundamental science may not require such assurance, they do however also follow to strict ethics rules related to scientific misconduct or fraud such as data fabrication, data falsification. For clinical research papers, you have to provide information on how the participants were selected, identify the possible sources of bias and confounding factors and how they were diminished.

In terms of the measurements, you have to clearly identify the materials used as well as the suppliers with their location. You should also be unambiguous when describing the analytical method. If the method has already been published, give a brief account and refer to the original publication (not a review in which the method is mentioned without a description). If you have modified it, you have to provide a detailed account of the modifications and you have to validate its accuracy, precision and repeatability. Mention the units in which results are reported and, if necessary, include the conversion factors [mass units versus “système international” (S.I.)]. In clinical research, surrogate end-points are often used as biomarkers. Under those circumstances, you must show their validity or refer to a study that has already shown that are valid.

In cases of clinical trials, the Methods section should include the study design, the patient selection mode, interventions, type of outcomes.

Statistics are important in assuring the quality of the research project. Hence, you should consult a biostatistician at the time of devising the research protocol and not after having performed the experiments or the clinical trial.

The components of the section on statistics should include:

• The way the data will be reported (mean, median, centiles for continuous data);
• Details on participant assignments to the different groups (random allocation, consecutive entry);
• Statistical comparison tools (parametric or non parametric statistics, paired or unpaired t-tests for normally distributed data and so on);
• The statistical power calculation when determining the sample size to obtain valid and significant comparisons together with the a level;
• The statistical software package used in the analysis.

Results section

The main purpose of the results section is to report the data that were collected and their relationship. It should also provide information on the modifications that have taken place because of unforeseen events leading to a modification of the initial protocol (loss of participants, reagent substitution, loss of data).

• Report results as tables and figures whenever possible, avoid duplication in the text. The text should summarize the findings;
• Report the data with the appropriate descriptive statistics;
• Report any unanticipated events that could affect the results;
• Report a complete account of observations and explanations for missing data (patient lost).

The discussion should set your research in context, reinforce its importance and show how your results have contributed to the further understanding of the problem posed. This should appear in the concluding remarks. The following organization could be helpful.

• Briefly summarize the main results of your study in one or two paragraphs, and how they support your working hypothesis;
• Provide an interpretation of your results and show how they logically fit in an overall scheme (biological or clinical);
• Describe how your results compare with those of other investigators, explain the differences observed;
• Discuss how your results may lead to a new hypothesis and further experimentation, or how they could enhance the diagnostic procedures.
• Provide the limitations of your study and steps taken to reduce them. This could be placed in the concluding remarks.

Acknowledgements

The acknowledgements are important as they identify and thank the contributors to the study, who do not meet the criteria as co-authors. They also include the recognition of the granting agency. In this case the grant award number and source is usually included.

Declaration of competing interests

Competing interests arise when the author has more than one role that may lead to a situation where there is a conflict of interest. This is observed when the investigator has a simultaneous industrial consulting and academic position. In that case the results may not be agreeable to the industrial sponsor, who may impose a veto on publication or strongly suggest modifications to the conclusions. The investigator must clear this issue before starting the contracted research. In addition, the investigator may own shares or stock in the company whose product forms the basis of the study. Such conflicts of interest must be declared so that they are apparent to the readers.

Acknowledgments

The authors thank Thomas A Lang, for his advice in the preparation of this manuscript.

The Complete MD/PhD Applicant Guide pp 61–65 Cite as

The Significant Research Experience Essay

• Jonathan Sussman 4 ,
• Jordan Setayesh 5 &
• Amitej Venapally 6  
• First Online: 23 September 2020

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One of the most unique and substantial parts of the MD/PhD application is the significant research experience essay, which is a part of the primary AMCAS application. In this essay, applicants describe their research positions, projects, and accomplishments in detail. This chapter explains how to structure and write this essay in terms of the scientific method. It is important for applicants to demonstrate involvement in every step of the scientific process, from designing the experiments to communicating the results. However, this essay serves as an excellent medium through which applicants can explain how their research interests have evolved over time and the most important lessons they learned through research. In effect, this essay can be viewed as a technical-based personal statement.

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Sussman, J., Setayesh, J., Venapally, A. (2021). The Significant Research Experience Essay. In: The Complete MD/PhD Applicant Guide. Springer, Cham. https://doi.org/10.1007/978-3-030-55625-9_8

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Tools & Methods

How to successfully write a scientific essay.

Posted by Cody Rhodes

If you are undertaking a course which relates to science, you are more or less apt to write an essay on science. You need to know how to write a science essay irrespective of whether your professor gives you a topic or you come up with one. Additionally, you need to have an end objective in mind. Writing a science essay necessitates that you produce an article which has all the details and facts about the subject matter and it ought to be to the point. Also, you need to know and understand that science essays are more or less different from other types of essays. They require you to be analytical and precise when answering questions. Hence, this can be quite challenging and tiresome. However, that should not deter you from learning how to write your paper. You can always inquire for pre-written research papers for sale from writing services like EssayZoo.

Also, you can read other people’s articles and find out how they produce and develop unique and high-quality papers. Moreover, this will help you understand how to approach your essays in different ways. Nonetheless, if you want to learn how to write a scientific paper in a successful manner, consider the following tips.

Select a topic for your article Like any other type of essay, you need to have a topic before you start the actual writing process. Your professor or instructor may give you a science essay topic to write about or ask you to come up with yours. When selecting a topic for your paper, ensure that you choose one you can write about. Do not pick a complex topic which can make the writing process boring and infuriating for you. Instead, choose one that you are familiar with. Select a topic you will not struggle gathering information about. Also, you need to have an interest in it. If you are unable to come up with a good topic, trying reading other people’s articles. This will help you develop a topic with ease.

Draft a plan After selecting a topic, the next step is drafting a plan or an outline. An outline is fundamental in writing a scientific essay as it is the foundation on which your paper is built. Additionally, it acts as a road map for your article. Hence, you need to incorporate all the thoughts and ideas you will include in your essay in the outline. You need to know what you will include in the introduction, the body, and the conclusion. Drafting a plan helps you save a lot of time when writing your paper. Also, it helps you to keep track of the primary objective of your article.

Start writing the article After drafting a plan, you can begin the writing process. Writing your paper will be smooth and easier as you have an outline which helps simplify the writing process. When writing your article, begin with a strong hook for your introduction. Dictate the direction your paper will take. Provide some background information and state the issue you will discuss as well as the solutions you have come up with. Arrange the body of your article according to the essay structure you will use to guide you. Also, ensure that you use transitory sentences to show the relationship between the paragraphs of your article. Conclude your essay by summarizing all the key points. Also, highlight the practical potential of our findings and their impacts.

Proofread and check for errors in the paper Before submitting or forwarding your article, it is fundamental that you proofread and correct all the errors that you come across. Delivering a paper that is full of mistakes can affect your overall performance in a negative manner. Thus, it is essential you revise your paper and check for errors. Correct all of them. Ask a friend to proofread your paper. He or she may spot some of the mistakes you did not come across.

In conclusion, writing a scientific essay differs from writing other types of papers. A scientific essay requires you to produce an article which has all the information and facts about the subject matter and it ought to be to the point. Nonetheless, the scientific essay formats similar to the format of any other essay: introduction, body, and conclusion. You need to use your outline to guide you through the writing process. To learn how to write a scientific essay in a successful manner, consider the tips above.

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• How to Write Your Methods

Ensure understanding, reproducibility and replicability

What should you include in your methods section, and how much detail is appropriate?

Why Methods Matter

The methods section was once the most likely part of a paper to be unfairly abbreviated, overly summarized, or even relegated to hard-to-find sections of a publisher’s website. While some journals may responsibly include more detailed elements of methods in supplementary sections, the movement for increased reproducibility and rigor in science has reinstated the importance of the methods section. Methods are now viewed as a key element in establishing the credibility of the research being reported, alongside the open availability of data and results.

A clear methods section impacts editorial evaluation and readers’ understanding, and is also the backbone of transparency and replicability.

For example, the Reproducibility Project: Cancer Biology project set out in 2013 to replicate experiments from 50 high profile cancer papers, but revised their target to 18 papers once they understood how much methodological detail was not contained in the original papers.

What to include in your methods section

What you include in your methods sections depends on what field you are in and what experiments you are performing. However, the general principle in place at the majority of journals is summarized well by the guidelines at PLOS ONE : “The Materials and Methods section should provide enough detail to allow suitably skilled investigators to fully replicate your study. ” The emphases here are deliberate: the methods should enable readers to understand your paper, and replicate your study. However, there is no need to go into the level of detail that a lay-person would require—the focus is on the reader who is also trained in your field, with the suitable skills and knowledge to attempt a replication.

A constant principle of rigorous science

A methods section that enables other researchers to understand and replicate your results is a constant principle of rigorous, transparent, and Open Science. Aim to be thorough, even if a particular journal doesn’t require the same level of detail . Reproducibility is all of our responsibility. You cannot create any problems by exceeding a minimum standard of information. If a journal still has word-limits—either for the overall article or specific sections—and requires some methodological details to be in a supplemental section, that is OK as long as the extra details are searchable and findable .

Imagine replicating your own work, years in the future

As part of PLOS’ presentation on Reproducibility and Open Publishing (part of UCSF’s Reproducibility Series ) we recommend planning the level of detail in your methods section by imagining you are writing for your future self, replicating your own work. When you consider that you might be at a different institution, with different account logins, applications, resources, and access levels—you can help yourself imagine the level of specificity that you yourself would require to redo the exact experiment. Consider:

• Which details would you need to be reminded of? 
• Which cell line, or antibody, or software, or reagent did you use, and does it have a Research Resource ID (RRID) that you can cite?
• Which version of a questionnaire did you use in your survey? 
• Exactly which visual stimulus did you show participants, and is it publicly available? 
• What participants did you decide to exclude? 
• What process did you adjust, during your work? 

Tip: Be sure to capture any changes to your protocols

You yourself would want to know about any adjustments, if you ever replicate the work, so you can surmise that anyone else would want to as well. Even if a necessary adjustment you made was not ideal, transparency is the key to ensuring this is not regarded as an issue in the future. It is far better to transparently convey any non-optimal methods, or methodological constraints, than to conceal them, which could result in reproducibility or ethical issues downstream.

Visual aids for methods help when reading the whole paper

Consider whether a visual representation of your methods could be appropriate or aid understanding your process. A visual reference readers can easily return to, like a flow-diagram, decision-tree, or checklist, can help readers to better understand the complete article, not just the methods section.

Ethical Considerations

In addition to describing what you did, it is just as important to assure readers that you also followed all relevant ethical guidelines when conducting your research. While ethical standards and reporting guidelines are often presented in a separate section of a paper, ensure that your methods and protocols actually follow these guidelines. Read more about ethics .

Existing standards, checklists, guidelines, partners

While the level of detail contained in a methods section should be guided by the universal principles of rigorous science outlined above, various disciplines, fields, and projects have worked hard to design and develop consistent standards, guidelines, and tools to help with reporting all types of experiment. Below, you’ll find some of the key initiatives. Ensure you read the submission guidelines for the specific journal you are submitting to, in order to discover any further journal- or field-specific policies to follow, or initiatives/tools to utilize.

Tip: Keep your paper moving forward by providing the proper paperwork up front

Be sure to check the journal guidelines and provide the necessary documents with your manuscript submission. Collecting the necessary documentation can greatly slow the first round of peer review, or cause delays when you submit your revision.

Randomized Controlled Trials – CONSORT The Consolidated Standards of Reporting Trials (CONSORT) project covers various initiatives intended to prevent the problems of  inadequate reporting of randomized controlled trials. The primary initiative is an evidence-based minimum set of recommendations for reporting randomized trials known as the CONSORT Statement . 

Systematic Reviews and Meta-Analyses – PRISMA The Preferred Reporting Items for Systematic Reviews and Meta-Analyses ( PRISMA ) is an evidence-based minimum set of items focusing  on the reporting of  reviews evaluating randomized trials and other types of research.

Research using Animals – ARRIVE The Animal Research: Reporting of In Vivo Experiments ( ARRIVE ) guidelines encourage maximizing the information reported in research using animals thereby minimizing unnecessary studies. (Original study and proposal , and updated guidelines , in PLOS Biology .) 

Laboratory Protocols Protocols.io has developed a platform specifically for the sharing and updating of laboratory protocols , which are assigned their own DOI and can be linked from methods sections of papers to enhance reproducibility. Contextualize your protocol and improve discovery with an accompanying Lab Protocol article in PLOS ONE .

Consistent reporting of Materials, Design, and Analysis – the MDAR checklist A cross-publisher group of editors and experts have developed, tested, and rolled out a checklist to help establish and harmonize reporting standards in the Life Sciences . The checklist , which is available for use by authors to compile their methods, and editors/reviewers to check methods, establishes a minimum set of requirements in transparent reporting and is adaptable to any discipline within the Life Sciences, by covering a breadth of potentially relevant methodological items and considerations. If you are in the Life Sciences and writing up your methods section, try working through the MDAR checklist and see whether it helps you include all relevant details into your methods, and whether it reminded you of anything you might have missed otherwise.

Summary Writing tips

The main challenge you may find when writing your methods is keeping it readable AND covering all the details needed for reproducibility and replicability. While this is difficult, do not compromise on rigorous standards for credibility!

• Keep in mind future replicability, alongside understanding and readability.
• Follow checklists, and field- and journal-specific guidelines.
• Consider a commitment to rigorous and transparent science a personal responsibility, and not just adhering to journal guidelines.
• Establish whether there are persistent identifiers for any research resources you use that can be specifically cited in your methods section.
• Deposit your laboratory protocols in Protocols.io, establishing a permanent link to them. You can update your protocols later if you improve on them, as can future scientists who follow your protocols.
• Consider visual aids like flow-diagrams, lists, to help with reading other sections of the paper.
• Be specific about all decisions made during the experiments that someone reproducing your work would need to know.

Don’t

• Summarize or abbreviate methods without giving full details in a discoverable supplemental section.
• Presume you will always be able to remember how you performed the experiments, or have access to private or institutional notebooks and resources.
• Attempt to hide constraints or non-optimal decisions you had to make–transparency is the key to ensuring the credibility of your research.
• How to Write a Great Title
• How to Write an Abstract
• How to Report Statistics
• How to Write Discussions and Conclusions
• How to Edit Your Work

The contents of the Peer Review Center are also available as a live, interactive training session, complete with slides, talking points, and activities. …

The contents of the Writing Center are also available as a live, interactive training session, complete with slides, talking points, and activities. …

There’s a lot to consider when deciding where to submit your work. Learn how to choose a journal that will help your study reach its audience, while reflecting your values as a researcher…

How to Write a Scientific Essay

When writing any essay it’s important to always keep the end goal in mind. You want to produce a document that is detailed, factual, about the subject matter and most importantly to the point.

Writing scientific essays will always be slightly different to when you write an essay for say English Literature . You need to be more analytical and precise when answering your questions. To help achieve this, you need to keep three golden rules in mind.

• Analysing the question, so that you know exactly what you have to do

Planning your answer

• Writing the essay

Now, let’s look at these steps in more detail to help you fully understand how to apply the three golden rules.

Analysing the question

• Start by looking at the instruction. Essays need to be written out in continuous prose. You shouldn’t be using bullet points or writing in note form.
• If it helps to make a particular point, however, you can use a diagram providing it is relevant and adequately explained.
• Look at the topic you are required to write about. The wording of the essay title tells you what you should confine your answer to – there is no place for interesting facts about other areas.

The next step is to plan your answer. What we are going to try to do is show you how to produce an effective plan in a very short time. You need a framework to show your knowledge otherwise it is too easy to concentrate on only a few aspects.

For example, when writing an essay on biology we can divide the topic up in a number of different ways. So, if you have to answer a question like ‘Outline the main properties of life and system reproduction’

The steps for planning are simple. Firstly, define the main terms within the question that need to be addressed. Then list the properties asked for and lastly, roughly assess how many words of your word count you are going to allocate to each term.

Writing the Essay

The final step (you’re almost there), now you have your plan in place for the essay, it’s time to get it all down in black and white. Follow your plan for answering the question, making sure you stick to the word count, check your spelling and grammar and give credit where credit’s (always reference your sources).

How Tutors Breakdown Essays

An exceptional essay

• reflects the detail that could be expected from a comprehensive knowledge and understanding of relevant parts of the specification
• is free from fundamental errors
• maintains appropriate depth and accuracy throughout
• includes two or more paragraphs of material that indicates greater depth or breadth of study

A good essay

An average essay

• contains a significant amount of material that reflects the detail that could be expected from a knowledge and understanding of relevant parts of the specification.

In practice this will amount to about half the essay.

• is likely to reflect limited knowledge of some areas and to be patchy in quality
• demonstrates a good understanding of basic principles with some errors and evidence of misunderstanding

A poor essay

• contains much material which is below the level expected of a candidate who has completed the course
• Contains fundamental errors reflecting a poor grasp of basic principles and concepts

Privacy Overview

Essay and dissertation writing skills

Planning your essay

Writing your introduction

Structuring your essay

• Writing essays in science subjects
• Brief video guides to support essay planning and writing
• Writing extended essays and dissertations
• Planning your dissertation writing time

Structuring your dissertation

• Top tips for writing longer pieces of work

Advice on planning and writing essays and dissertations

University essays differ from school essays in that they are less concerned with what you know and more concerned with how you construct an argument to answer the question. This means that the starting point for writing a strong essay is to first unpick the question and to then use this to plan your essay before you start putting pen to paper (or finger to keyboard).

A really good starting point for you are these short, downloadable Tips for Successful Essay Writing and Answering the Question resources. Both resources will help you to plan your essay, as well as giving you guidance on how to distinguish between different sorts of essay questions. 

You may find it helpful to watch this seven-minute video on six tips for essay writing which outlines how to interpret essay questions, as well as giving advice on planning and structuring your writing:

Different disciplines will have different expectations for essay structure and you should always refer to your Faculty or Department student handbook or course Canvas site for more specific guidance.

However, broadly speaking, all essays share the following features:

Essays need an introduction to establish and focus the parameters of the discussion that will follow. You may find it helpful to divide the introduction into areas to demonstrate your breadth and engagement with the essay question. You might define specific terms in the introduction to show your engagement with the essay question; for example, ‘This is a large topic which has been variously discussed by many scientists and commentators. The principle tension is between the views of X and Y who define the main issues as…’ Breadth might be demonstrated by showing the range of viewpoints from which the essay question could be considered; for example, ‘A variety of factors including economic, social and political, influence A and B. This essay will focus on the social and economic aspects, with particular emphasis on…..’

Watch this two-minute video to learn more about how to plan and structure an introduction:

The main body of the essay should elaborate on the issues raised in the introduction and develop an argument(s) that answers the question. It should consist of a number of self-contained paragraphs each of which makes a specific point and provides some form of evidence to support the argument being made. Remember that a clear argument requires that each paragraph explicitly relates back to the essay question or the developing argument.

• Conclusion: An essay should end with a conclusion that reiterates the argument in light of the evidence you have provided; you shouldn’t use the conclusion to introduce new information.
• References: You need to include references to the materials you’ve used to write your essay. These might be in the form of footnotes, in-text citations, or a bibliography at the end. Different systems exist for citing references and different disciplines will use various approaches to citation. Ask your tutor which method(s) you should be using for your essay and also consult your Department or Faculty webpages for specific guidance in your discipline. 

Essay writing in science subjects

If you are writing an essay for a science subject you may need to consider additional areas, such as how to present data or diagrams. This five-minute video gives you some advice on how to approach your reading list, planning which information to include in your answer and how to write for your scientific audience – the video is available here:

A PDF providing further guidance on writing science essays for tutorials is available to download.

Short videos to support your essay writing skills

There are many other resources at Oxford that can help support your essay writing skills and if you are short on time, the Oxford Study Skills Centre has produced a number of short (2-minute) videos covering different aspects of essay writing, including:

• Approaching different types of essay questions  
• Structuring your essay  
• Writing an introduction  
• Making use of evidence in your essay writing  
• Writing your conclusion

Extended essays and dissertations

Longer pieces of writing like extended essays and dissertations may seem like quite a challenge from your regular essay writing. The important point is to start with a plan and to focus on what the question is asking. A PDF providing further guidance on planning Humanities and Social Science dissertations is available to download.

Planning your time effectively

Try not to leave the writing until close to your deadline, instead start as soon as you have some ideas to put down onto paper. Your early drafts may never end up in the final work, but the work of committing your ideas to paper helps to formulate not only your ideas, but the method of structuring your writing to read well and conclude firmly.

Although many students and tutors will say that the introduction is often written last, it is a good idea to begin to think about what will go into it early on. For example, the first draft of your introduction should set out your argument, the information you have, and your methods, and it should give a structure to the chapters and sections you will write. Your introduction will probably change as time goes on but it will stand as a guide to your entire extended essay or dissertation and it will help you to keep focused.

The structure of  extended essays or dissertations will vary depending on the question and discipline, but may include some or all of the following:

• The background information to - and context for - your research. This often takes the form of a literature review.
• Explanation of the focus of your work.
• Explanation of the value of this work to scholarship on the topic.
• List of the aims and objectives of the work and also the issues which will not be covered because they are outside its scope.

The main body of your extended essay or dissertation will probably include your methodology, the results of research, and your argument(s) based on your findings.

The conclusion is to summarise the value your research has added to the topic, and any further lines of research you would undertake given more time or resources. 

Tips on writing longer pieces of work

Approaching each chapter of a dissertation as a shorter essay can make the task of writing a dissertation seem less overwhelming. Each chapter will have an introduction, a main body where the argument is developed and substantiated with evidence, and a conclusion to tie things together. Unlike in a regular essay, chapter conclusions may also introduce the chapter that will follow, indicating how the chapters are connected to one another and how the argument will develop through your dissertation.

For further guidance, watch this two-minute video on writing longer pieces of work . 

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