what is hypothesis of a study

What Is A Research (Scientific) Hypothesis? A plain-language explainer + examples

By:  Derek Jansen (MBA)  | Reviewed By: Dr Eunice Rautenbach | June 2020

If you’re new to the world of research, or it’s your first time writing a dissertation or thesis, you’re probably noticing that the words “research hypothesis” and “scientific hypothesis” are used quite a bit, and you’re wondering what they mean in a research context .

“Hypothesis” is one of those words that people use loosely, thinking they understand what it means. However, it has a very specific meaning within academic research. So, it’s important to understand the exact meaning before you start hypothesizing. 

Research Hypothesis 101

• What is a hypothesis ?
• What is a research hypothesis (scientific hypothesis)?
• Requirements for a research hypothesis
• Definition of a research hypothesis
• The null hypothesis

What is a hypothesis?

Let’s start with the general definition of a hypothesis (not a research hypothesis or scientific hypothesis), according to the Cambridge Dictionary:

Hypothesis: an idea or explanation for something that is based on known facts but has not yet been proved.

In other words, it’s a statement that provides an explanation for why or how something works, based on facts (or some reasonable assumptions), but that has not yet been specifically tested . For example, a hypothesis might look something like this:

Hypothesis: sleep impacts academic performance.

This statement predicts that academic performance will be influenced by the amount and/or quality of sleep a student engages in – sounds reasonable, right? It’s based on reasonable assumptions , underpinned by what we currently know about sleep and health (from the existing literature). So, loosely speaking, we could call it a hypothesis, at least by the dictionary definition.

But that’s not good enough…

Unfortunately, that’s not quite sophisticated enough to describe a research hypothesis (also sometimes called a scientific hypothesis), and it wouldn’t be acceptable in a dissertation, thesis or research paper . In the world of academic research, a statement needs a few more criteria to constitute a true research hypothesis .

What is a research hypothesis?

A research hypothesis (also called a scientific hypothesis) is a statement about the expected outcome of a study (for example, a dissertation or thesis). To constitute a quality hypothesis, the statement needs to have three attributes – specificity , clarity and testability .

Let’s take a look at these more closely.

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Hypothesis Essential #1: Specificity & Clarity

A good research hypothesis needs to be extremely clear and articulate about both what’ s being assessed (who or what variables are involved ) and the expected outcome (for example, a difference between groups, a relationship between variables, etc.).

Let’s stick with our sleepy students example and look at how this statement could be more specific and clear.

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.

As you can see, the statement is very specific as it identifies the variables involved (sleep hours and test grades), the parties involved (two groups of students), as well as the predicted relationship type (a positive relationship). There’s no ambiguity or uncertainty about who or what is involved in the statement, and the expected outcome is clear.

Contrast that to the original hypothesis we looked at – “Sleep impacts academic performance” – and you can see the difference. “Sleep” and “academic performance” are both comparatively vague , and there’s no indication of what the expected relationship direction is (more sleep or less sleep). As you can see, specificity and clarity are key.

Hypothesis Essential #2: Testability (Provability)

A statement must be testable to qualify as a research hypothesis. In other words, there needs to be a way to prove (or disprove) the statement. If it’s not testable, it’s not a hypothesis – simple as that.

For example, consider the hypothesis we mentioned earlier:

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.  

We could test this statement by undertaking a quantitative study involving two groups of students, one that gets 8 or more hours of sleep per night for a fixed period, and one that gets less. We could then compare the standardised test results for both groups to see if there’s a statistically significant difference. 

Again, if you compare this to the original hypothesis we looked at – “Sleep impacts academic performance” – you can see that it would be quite difficult to test that statement, primarily because it isn’t specific enough. How much sleep? By who? What type of academic performance?

So, remember the mantra – if you can’t test it, it’s not a hypothesis 🙂

Defining A Research Hypothesis

You’re still with us? Great! Let’s recap and pin down a clear definition of a hypothesis.

A research hypothesis (or scientific hypothesis) is a statement about an expected relationship between variables, or explanation of an occurrence, that is clear, specific and testable.

So, when you write up hypotheses for your dissertation or thesis, make sure that they meet all these criteria. If you do, you’ll not only have rock-solid hypotheses but you’ll also ensure a clear focus for your entire research project.

What about the null hypothesis?

You may have also heard the terms null hypothesis , alternative hypothesis, or H-zero thrown around. At a simple level, the null hypothesis is the counter-proposal to the original hypothesis.

For example, if the hypothesis predicts that there is a relationship between two variables (for example, sleep and academic performance), the null hypothesis would predict that there is no relationship between those variables.

At a more technical level, the null hypothesis proposes that no statistical significance exists in a set of given observations and that any differences are due to chance alone.

And there you have it – hypotheses in a nutshell. 

If you have any questions, be sure to leave a comment below and we’ll do our best to help you. If you need hands-on help developing and testing your hypotheses, consider our private coaching service , where we hold your hand through the research journey.

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16 Comments

Very useful information. I benefit more from getting more information in this regard.

Very great insight,educative and informative. Please give meet deep critics on many research data of public international Law like human rights, environment, natural resources, law of the sea etc

In a book I read a distinction is made between null, research, and alternative hypothesis. As far as I understand, alternative and research hypotheses are the same. Can you please elaborate? Best Afshin

This is a self explanatory, easy going site. I will recommend this to my friends and colleagues.

Very good definition. How can I cite your definition in my thesis? Thank you. Is nul hypothesis compulsory in a research?

It’s a counter-proposal to be proven as a rejection

Please what is the difference between alternate hypothesis and research hypothesis?

It is a very good explanation. However, it limits hypotheses to statistically tasteable ideas. What about for qualitative researches or other researches that involve quantitative data that don’t need statistical tests?

In qualitative research, one typically uses propositions, not hypotheses.

could you please elaborate it more

I’ve benefited greatly from these notes, thank you.

This is very helpful

well articulated ideas are presented here, thank you for being reliable sources of information

Excellent. Thanks for being clear and sound about the research methodology and hypothesis (quantitative research)

I have only a simple question regarding the null hypothesis. – Is the null hypothesis (Ho) known as the reversible hypothesis of the alternative hypothesis (H1? – How to test it in academic research?

this is very important note help me much more

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

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• How to Write a Strong Hypothesis | Guide & Examples

How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Research Hypothesis In Psychology: Types, & Examples

Saul Mcleod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul Mcleod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

On This Page:

A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

• A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
• It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
• A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
• Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
• For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
• Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

• Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

• Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
• However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

• Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
• Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
• Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
• Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
• Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

• The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
• The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

• Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
• Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
• Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
• Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
• Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
• Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
• Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
• Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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How to Write a Research Hypothesis

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Since grade school, we've all been familiar with hypotheses. The hypothesis is an essential step of the scientific method. But what makes an effective research hypothesis, how do you create one, and what types of hypotheses are there? We answer these questions and more.

Updated on April 27, 2022

What is a research hypothesis?

General hypothesis.

Since grade school, we've all been familiar with the term “hypothesis.” A hypothesis is a fact-based guess or prediction that has not been proven. It is an essential step of the scientific method. The hypothesis of a study is a drive for experimentation to either prove the hypothesis or dispute it.

Research Hypothesis

A research hypothesis is more specific than a general hypothesis. It is an educated, expected prediction of the outcome of a study that is testable.

What makes an effective research hypothesis?

A good research hypothesis is a clear statement of the relationship between a dependent variable(s) and independent variable(s) relevant to the study that can be disproven.

Research hypothesis checklist

Once you've written a possible hypothesis, make sure it checks the following boxes:

• It must be testable: You need a means to prove your hypothesis. If you can't test it, it's not a hypothesis.
• It must include a dependent and independent variable: At least one independent variable ( cause ) and one dependent variable ( effect ) must be included.
• The language must be easy to understand: Be as clear and concise as possible. Nothing should be left to interpretation.
• It must be relevant to your research topic: You probably shouldn't be talking about cats and dogs if your research topic is outer space. Stay relevant to your topic.

How to create an effective research hypothesis

Pose it as a question first.

Start your research hypothesis from a journalistic approach. Ask one of the five W's: Who, what, when, where, or why.

A possible initial question could be: Why is the sky blue?

Do the preliminary research

Once you have a question in mind, read research around your topic. Collect research from academic journals.

If you're looking for information about the sky and why it is blue, research information about the atmosphere, weather, space, the sun, etc.

Write a draft hypothesis

Once you're comfortable with your subject and have preliminary knowledge, create a working hypothesis. Don't stress much over this. Your first hypothesis is not permanent. Look at it as a draft.

Your first draft of a hypothesis could be: Certain molecules in the Earth's atmosphere are responsive to the sky being the color blue.

Make your working draft perfect

Take your working hypothesis and make it perfect. Narrow it down to include only the information listed in the “Research hypothesis checklist” above.

Now that you've written your working hypothesis, narrow it down. Your new hypothesis could be: Light from the sun hitting oxygen molecules in the sky makes the color of the sky appear blue.

Write a null hypothesis

Your null hypothesis should be the opposite of your research hypothesis. It should be able to be disproven by your research.

In this example, your null hypothesis would be: Light from the sun hitting oxygen molecules in the sky does not make the color of the sky appear blue.

Why is it important to have a clear, testable hypothesis?

One of the main reasons a manuscript can be rejected from a journal is because of a weak hypothesis. “Poor hypothesis, study design, methodology, and improper use of statistics are other reasons for rejection of a manuscript,” says Dr. Ish Kumar Dhammi and Dr. Rehan-Ul-Haq in Indian Journal of Orthopaedics.

According to Dr. James M. Provenzale in American Journal of Roentgenology , “The clear declaration of a research question (or hypothesis) in the Introduction is critical for reviewers to understand the intent of the research study. It is best to clearly state the study goal in plain language (for example, “We set out to determine whether condition x produces condition y.”) An insufficient problem statement is one of the more common reasons for manuscript rejection.”

Characteristics that make a hypothesis weak include:

• Unclear variables
• Unoriginality
• Too general
• Too specific

A weak hypothesis leads to weak research and methods . The goal of a paper is to prove or disprove a hypothesis - or to prove or disprove a null hypothesis. If the hypothesis is not a dependent variable of what is being studied, the paper's methods should come into question.

A strong hypothesis is essential to the scientific method. A hypothesis states an assumed relationship between at least two variables and the experiment then proves or disproves that relationship with statistical significance. Without a proven and reproducible relationship, the paper feeds into the reproducibility crisis. Learn more about writing for reproducibility .

In a study published in The Journal of Obstetrics and Gynecology of India by Dr. Suvarna Satish Khadilkar, she reviewed 400 rejected manuscripts to see why they were rejected. Her studies revealed that poor methodology was a top reason for the submission having a final disposition of rejection.

Aside from publication chances, Dr. Gareth Dyke believes a clear hypothesis helps efficiency.

“Developing a clear and testable hypothesis for your research project means that you will not waste time, energy, and money with your work,” said Dyke. “Refining a hypothesis that is both meaningful, interesting, attainable, and testable is the goal of all effective research.”

Types of research hypotheses

There can be overlap in these types of hypotheses.

Simple hypothesis

A simple hypothesis is a hypothesis at its most basic form. It shows the relationship of one independent and one independent variable.

Example: Drinking soda (independent variable) every day leads to obesity (dependent variable).

Complex hypothesis

A complex hypothesis shows the relationship of two or more independent and dependent variables.

Example: Drinking soda (independent variable) every day leads to obesity (dependent variable) and heart disease (dependent variable).

Directional hypothesis

A directional hypothesis guesses which way the results of an experiment will go. It uses words like increase, decrease, higher, lower, positive, negative, more, or less. It is also frequently used in statistics.

Example: Humans exposed to radiation have a higher risk of cancer than humans not exposed to radiation.

Non-directional hypothesis

A non-directional hypothesis says there will be an effect on the dependent variable, but it does not say which direction.

Associative hypothesis

An associative hypothesis says that when one variable changes, so does the other variable.

Alternative hypothesis

An alternative hypothesis states that the variables have a relationship.

• The opposite of a null hypothesis

Example: An apple a day keeps the doctor away.

Null hypothesis

A null hypothesis states that there is no relationship between the two variables. It is posed as the opposite of what the alternative hypothesis states.

Researchers use a null hypothesis to work to be able to reject it. A null hypothesis:

• Can never be proven
• Can only be rejected
• Is the opposite of an alternative hypothesis

Example: An apple a day does not keep the doctor away.

Logical hypothesis

A logical hypothesis is a suggested explanation while using limited evidence.

Example: Bats can navigate in the dark better than tigers.

In this hypothesis, the researcher knows that tigers cannot see in the dark, and bats mostly live in darkness.

Empirical hypothesis

An empirical hypothesis is also called a “working hypothesis.” It uses the trial and error method and changes around the independent variables.

• An apple a day keeps the doctor away.
• Two apples a day keep the doctor away.
• Three apples a day keep the doctor away.

In this case, the research changes the hypothesis as the researcher learns more about his/her research.

Statistical hypothesis

A statistical hypothesis is a look of a part of a population or statistical model. This type of hypothesis is especially useful if you are making a statement about a large population. Instead of having to test the entire population of Illinois, you could just use a smaller sample of people who live there.

Example: 70% of people who live in Illinois are iron deficient.

Causal hypothesis

A causal hypothesis states that the independent variable will have an effect on the dependent variable.

Example: Using tobacco products causes cancer.

Final thoughts

Make sure your research is error-free before you send it to your preferred journal . Check our our English Editing services to avoid your chances of desk rejection.

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The Research Hypothesis: Role and Construction

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A hypothesis is a logical construct, interposed between a problem and its solution, which represents a proposed answer to a research question. It gives direction to the investigator’s thinking about the problem and, therefore, facilitates a solution. There are three primary modes of inference by which hypotheses are developed: deduction (reasoning from a general propositions to specific instances), induction (reasoning from specific instances to a general proposition), and abduction (formulation/acceptance on probation of a hypothesis to explain a surprising observation).

A research hypothesis should reflect an inference about variables; be stated as a grammatically complete, declarative sentence; be expressed simply and unambiguously; provide an adequate answer to the research problem; and be testable. Hypotheses can be classified as conceptual versus operational, single versus bi- or multivariable, causal or not causal, mechanistic versus nonmechanistic, and null or alternative. Hypotheses most commonly entail statements about “variables” which, in turn, can be classified according to their level of measurement (scaling characteristics) or according to their role in the hypothesis (independent, dependent, moderator, control, or intervening).

A hypothesis is rendered operational when its broadly (conceptually) stated variables are replaced by operational definitions of those variables. Hypotheses stated in this manner are called operational hypotheses, specific hypotheses, or predictions and facilitate testing.

Wrong hypotheses, rightly worked from, have produced more results than unguided observation

—Augustus De Morgan, 1872[ 1 ]—

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Supino, P.G. (2012). The Research Hypothesis: Role and Construction. In: Supino, P., Borer, J. (eds) Principles of Research Methodology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3360-6_3

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How to Write a Hypothesis – Steps & Tips

Published by Alaxendra Bets at August 14th, 2021 , Revised On October 26, 2023

What is a Research Hypothesis?

You can test a research statement with the help of experimental or theoretical research, known as a hypothesis.

If you want to find out the similarities, differences, and relationships between variables, you must write a testable hypothesis before compiling the data, performing analysis, and generating results to complete.

The data analysis and findings will help you test the hypothesis and see whether it is true or false. Here is all you need to know about how to write a hypothesis for a  dissertation .

Research Hypothesis Definition

Not sure what the meaning of the research hypothesis is?

A research hypothesis predicts an answer to the research question  based on existing theoretical knowledge or experimental data.

Some studies may have multiple hypothesis statements depending on the research question(s).  A research hypothesis must be based on formulas, facts, and theories. It should be testable by data analysis, observations, experiments, or other scientific methodologies that can refute or support the statement.

Variables in Hypothesis

Developing a hypothesis is easy. Most research studies have two or more variables in the hypothesis, particularly studies involving correlational and experimental research. The researcher can control or change the independent variable(s) while measuring and observing the independent variable(s).

“How long a student sleeps affects test scores.”

In the above statement, the dependent variable is the test score, while the independent variable is the length of time spent in sleep. Developing a hypothesis will be easy if you know your research’s dependent and independent variables.

Once you have developed a thesis statement, questions such as how to write a hypothesis for the dissertation and how to test a research hypothesis become pretty straightforward.

Looking for dissertation help?

Researchprospect to the rescue then.

We have expert writers on our team who are skilled at helping students with quantitative dissertations across a variety of STEM disciplines. Guaranteeing 100% satisfaction!

Step-by-Step Guide on How to Write a Hypothesis

Here are the steps involved in how to write a hypothesis for a dissertation.

Step 1: Start with a Research Question

• Begin by asking a specific question about a topic of interest.
• This question should be clear, concise, and researchable.

Example: Does exposure to sunlight affect plant growth?

Step 2: Do Preliminary Research

• Before formulating a hypothesis, conduct background research to understand existing knowledge on the topic.
• Familiarise yourself with prior studies, theories, or observations related to the research question.

Step 3: Define Variables

• Independent Variable (IV): The factor that you change or manipulate in an experiment.
• Dependent Variable (DV): The factor that you measure.

Example: IV: Amount of sunlight exposure (e.g., 2 hours/day, 4 hours/day, 8 hours/day) DV: Plant growth (e.g., height in centimetres)

Step 4: Formulate the Hypothesis

• A hypothesis is a statement that predicts the relationship between variables.
• It is often written as an “if-then” statement.

Example: If plants receive more sunlight, then they will grow taller.

Step 5: Ensure it is Testable

A good hypothesis is empirically testable. This means you should be able to design an experiment or observation to test its validity.

Example: You can set up an experiment where plants are exposed to varying amounts of sunlight and then measure their growth over a period of time.

Step 6: Consider Potential Confounding Variables

• Confounding variables are factors other than the independent variable that might affect the outcome.
• It is important to identify these to ensure that they do not skew your results.

Example: Soil quality, water frequency, or type of plant can all affect growth. Consider keeping these constant in your experiment.

Step 7: Write the Null Hypothesis

• The null hypothesis is a statement that there is no effect or no relationship between the variables.
• It is what you aim to disprove or reject through your research.

Example: There is no difference in plant growth regardless of the amount of sunlight exposure.

Step 8: Test your Hypothesis

Design an experiment or conduct observations to test your hypothesis.

Example: Grow three sets of plants: one set exposed to 2 hours of sunlight daily, another exposed to 4 hours, and a third exposed to 8 hours. Measure and compare their growth after a set period.

Step 9: Analyse the Results

After testing, review your data to determine if it supports your hypothesis.

Step 10: Draw Conclusions

• Based on your findings, determine whether you can accept or reject the hypothesis.
• Remember, even if you reject your hypothesis, it’s a valuable result. It can guide future research and refine questions.

Three Ways to Phrase a Hypothesis

Try to use “if”… and “then”… to identify the variables. The independent variable should be present in the first part of the hypothesis, while the dependent variable will form the second part of the statement. Consider understanding the below research hypothesis example to create a specific, clear, and concise research hypothesis;

If an obese lady starts attending Zomba fitness classes, her health will improve.

In academic research, you can write the predicted variable relationship directly because most research studies correlate terms.

The number of Zomba fitness classes attended by the obese lady has a positive effect on health.

If your research compares two groups, then you can develop a hypothesis statement on their differences.

An obese lady who attended most Zumba fitness classes will have better health than those who attended a few.

How to Write a Null Hypothesis

If a statistical analysis is involved in your research, then you must create a null hypothesis. If you find any relationship between the variables, then the null hypothesis will be the default position that there is no relationship between them. H0 is the symbol for the null hypothesis, while the hypothesis is represented as H1. The null hypothesis will also answer your question, “How to test the research hypothesis in the dissertation.”

H0: The number of Zumba fitness classes attended by the obese lady does not affect her health.

H1: The number of Zumba fitness classes attended by obese lady positively affects health.

Also see:  Your Dissertation in Education

Hypothesis Examples

Research Question: Does the amount of sunlight a plant receives affect its growth? Hypothesis: Plants that receive more sunlight will grow taller than plants that receive less sunlight.

Research Question: Do students who eat breakfast perform better in school exams than those who don’t? Hypothesis: Students who eat a morning breakfast will score higher on school exams compared to students who skip breakfast.

Research Question: Does listening to music while studying impact a student’s ability to retain information? Hypothesis 1 (Directional): Students who listen to music while studying will retain less information than those who study in silence. Hypothesis 2 (Non-directional): There will be a difference in information retention between students who listen to music while studying and those who study in silence.

How can ResearchProspect Help?

If you are unsure about how to rest a research hypothesis in a dissertation or simply unsure about how to develop a hypothesis for your research, then you can take advantage of our dissertation services which cover every tiny aspect of a dissertation project you might need help with including but not limited to setting up a hypothesis and research questions,  help with individual chapters ,  full dissertation writing ,  statistical analysis , and much more.

Frequently Asked Questions

What are the 5 rules for writing a good hypothesis.

• Clear Statement: State a clear relationship between variables.
• Testable: Ensure it can be investigated and measured.
• Specific: Avoid vague terms, be precise in predictions.
• Falsifiable: Design to allow potential disproof.
• Relevant: Address research question and align with existing knowledge.

What is a hypothesis in simple words?

A hypothesis is an educated guess or prediction about something that can be tested. It is a statement that suggests a possible explanation for an event or phenomenon based on prior knowledge or observation. Scientists use hypotheses as a starting point for experiments to discover if they are true or false.

What is the hypothesis and examples?

A hypothesis is a testable prediction or explanation for an observation or phenomenon. For example, if plants are given sunlight, then they will grow. In this case, the hypothesis suggests that sunlight has a positive effect on plant growth. It can be tested by experimenting with plants in varying light conditions.

What is the hypothesis in research definition?

A hypothesis in research is a clear, testable statement predicting the possible outcome of a study based on prior knowledge and observation. It serves as the foundation for conducting experiments or investigations. Researchers test the validity of the hypothesis to draw conclusions and advance knowledge in a particular field.

Why is it called a hypothesis?

The term “hypothesis” originates from the Greek word “hypothesis,” which means “base” or “foundation.” It’s used to describe a foundational statement or proposition that can be tested. In scientific contexts, it denotes a tentative explanation for a phenomenon, serving as a starting point for investigation or experimentation.

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What is and How to Write a Good Hypothesis in Research?

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Table of Contents

One of the most important aspects of conducting research is constructing a strong hypothesis. But what makes a hypothesis in research effective? In this article, we’ll look at the difference between a hypothesis and a research question, as well as the elements of a good hypothesis in research. We’ll also include some examples of effective hypotheses, and what pitfalls to avoid.

What is a Hypothesis in Research?

Simply put, a hypothesis is a research question that also includes the predicted or expected result of the research. Without a hypothesis, there can be no basis for a scientific or research experiment. As such, it is critical that you carefully construct your hypothesis by being deliberate and thorough, even before you set pen to paper. Unless your hypothesis is clearly and carefully constructed, any flaw can have an adverse, and even grave, effect on the quality of your experiment and its subsequent results.

Research Question vs Hypothesis

It’s easy to confuse research questions with hypotheses, and vice versa. While they’re both critical to the Scientific Method, they have very specific differences. Primarily, a research question, just like a hypothesis, is focused and concise. But a hypothesis includes a prediction based on the proposed research, and is designed to forecast the relationship of and between two (or more) variables. Research questions are open-ended, and invite debate and discussion, while hypotheses are closed, e.g. “The relationship between A and B will be C.”

A hypothesis is generally used if your research topic is fairly well established, and you are relatively certain about the relationship between the variables that will be presented in your research. Since a hypothesis is ideally suited for experimental studies, it will, by its very existence, affect the design of your experiment. The research question is typically used for new topics that have not yet been researched extensively. Here, the relationship between different variables is less known. There is no prediction made, but there may be variables explored. The research question can be casual in nature, simply trying to understand if a relationship even exists, descriptive or comparative.

How to Write Hypothesis in Research

Writing an effective hypothesis starts before you even begin to type. Like any task, preparation is key, so you start first by conducting research yourself, and reading all you can about the topic that you plan to research. From there, you’ll gain the knowledge you need to understand where your focus within the topic will lie.

Remember that a hypothesis is a prediction of the relationship that exists between two or more variables. Your job is to write a hypothesis, and design the research, to “prove” whether or not your prediction is correct. A common pitfall is to use judgments that are subjective and inappropriate for the construction of a hypothesis. It’s important to keep the focus and language of your hypothesis objective.

An effective hypothesis in research is clearly and concisely written, and any terms or definitions clarified and defined. Specific language must also be used to avoid any generalities or assumptions.

Use the following points as a checklist to evaluate the effectiveness of your research hypothesis:

• Predicts the relationship and outcome
• Simple and concise – avoid wordiness
• Clear with no ambiguity or assumptions about the readers’ knowledge
• Observable and testable results
• Relevant and specific to the research question or problem

Research Hypothesis Example

Perhaps the best way to evaluate whether or not your hypothesis is effective is to compare it to those of your colleagues in the field. There is no need to reinvent the wheel when it comes to writing a powerful research hypothesis. As you’re reading and preparing your hypothesis, you’ll also read other hypotheses. These can help guide you on what works, and what doesn’t, when it comes to writing a strong research hypothesis.

Here are a few generic examples to get you started.

Eating an apple each day, after the age of 60, will result in a reduction of frequency of physician visits.

Budget airlines are more likely to receive more customer complaints. A budget airline is defined as an airline that offers lower fares and fewer amenities than a traditional full-service airline. (Note that the term “budget airline” is included in the hypothesis.

Workplaces that offer flexible working hours report higher levels of employee job satisfaction than workplaces with fixed hours.

Each of the above examples are specific, observable and measurable, and the statement of prediction can be verified or shown to be false by utilizing standard experimental practices. It should be noted, however, that often your hypothesis will change as your research progresses.

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Research Hypothesis: What It Is, Types + How to Develop?

A research study starts with a question. Researchers worldwide ask questions and create research hypotheses. The effectiveness of research relies on developing a good research hypothesis. Examples of research hypotheses can guide researchers in writing effective ones.

In this blog, we’ll learn what a research hypothesis is, why it’s important in research, and the different types used in science. We’ll also guide you through creating your research hypothesis and discussing ways to test and evaluate it.

What is a Research Hypothesis?

A hypothesis is like a guess or idea that you suggest to check if it’s true. A research hypothesis is a statement that brings up a question and predicts what might happen.

It’s really important in the scientific method and is used in experiments to figure things out. Essentially, it’s an educated guess about how things are connected in the research.

A research hypothesis usually includes pointing out the independent variable (the thing they’re changing or studying) and the dependent variable (the result they’re measuring or watching). It helps plan how to gather and analyze data to see if there’s evidence to support or deny the expected connection between these variables.

Importance of Hypothesis in Research

Hypotheses are really important in research. They help design studies, allow for practical testing, and add to our scientific knowledge. Their main role is to organize research projects, making them purposeful, focused, and valuable to the scientific community. Let’s look at some key reasons why they matter:

• A research hypothesis helps test theories.

A hypothesis plays a pivotal role in the scientific method by providing a basis for testing existing theories. For example, a hypothesis might test the predictive power of a psychological theory on human behavior.

• It serves as a great platform for investigation activities.

It serves as a launching pad for investigation activities, which offers researchers a clear starting point. A research hypothesis can explore the relationship between exercise and stress reduction.

• Hypothesis guides the research work or study.

A well-formulated hypothesis guides the entire research process. It ensures that the study remains focused and purposeful. For instance, a hypothesis about the impact of social media on interpersonal relationships provides clear guidance for a study.

• Hypothesis sometimes suggests theories.

In some cases, a hypothesis can suggest new theories or modifications to existing ones. For example, a hypothesis testing the effectiveness of a new drug might prompt a reconsideration of current medical theories.

• It helps in knowing the data needs.

A hypothesis clarifies the data requirements for a study, ensuring that researchers collect the necessary information—a hypothesis guiding the collection of demographic data to analyze the influence of age on a particular phenomenon.

• The hypothesis explains social phenomena.

Hypotheses are instrumental in explaining complex social phenomena. For instance, a hypothesis might explore the relationship between economic factors and crime rates in a given community.

• Hypothesis provides a relationship between phenomena for empirical Testing.

Hypotheses establish clear relationships between phenomena, paving the way for empirical testing. An example could be a hypothesis exploring the correlation between sleep patterns and academic performance.

• It helps in knowing the most suitable analysis technique.

A hypothesis guides researchers in selecting the most appropriate analysis techniques for their data. For example, a hypothesis focusing on the effectiveness of a teaching method may lead to the choice of statistical analyses best suited for educational research.

Characteristics of a Good Research Hypothesis

A hypothesis is a specific idea that you can test in a study. It often comes from looking at past research and theories. A good hypothesis usually starts with a research question that you can explore through background research. For it to be effective, consider these key characteristics:

• Clear and Focused Language: A good hypothesis uses clear and focused language to avoid confusion and ensure everyone understands it.
• Related to the Research Topic: The hypothesis should directly relate to the research topic, acting as a bridge between the specific question and the broader study.
• Testable: An effective hypothesis can be tested, meaning its prediction can be checked with real data to support or challenge the proposed relationship.
• Potential for Exploration: A good hypothesis often comes from a research question that invites further exploration. Doing background research helps find gaps and potential areas to investigate.
• Includes Variables: The hypothesis should clearly state both the independent and dependent variables, specifying the factors being studied and the expected outcomes.
• Ethical Considerations: Check if variables can be manipulated without breaking ethical standards. It’s crucial to maintain ethical research practices.
• Predicts Outcomes: The hypothesis should predict the expected relationship and outcome, acting as a roadmap for the study and guiding data collection and analysis.
• Simple and Concise: A good hypothesis avoids unnecessary complexity and is simple and concise, expressing the essence of the proposed relationship clearly.
• Clear and Assumption-Free: The hypothesis should be clear and free from assumptions about the reader’s prior knowledge, ensuring universal understanding.
• Observable and Testable Results: A strong hypothesis implies research that produces observable and testable results, making sure the study’s outcomes can be effectively measured and analyzed.

When you use these characteristics as a checklist, it can help you create a good research hypothesis. It’ll guide improving and strengthening the hypothesis, identifying any weaknesses, and making necessary changes. Crafting a hypothesis with these features helps you conduct a thorough and insightful research study.

Types of Research Hypotheses

The research hypothesis comes in various types, each serving a specific purpose in guiding the scientific investigation. Knowing the differences will make it easier for you to create your own hypothesis. Here’s an overview of the common types:

01. Null Hypothesis

The null hypothesis states that there is no connection between two considered variables or that two groups are unrelated. As discussed earlier, a hypothesis is an unproven assumption lacking sufficient supporting data. It serves as the statement researchers aim to disprove. It is testable, verifiable, and can be rejected.

For example, if you’re studying the relationship between Project A and Project B, assuming both projects are of equal standard is your null hypothesis. It needs to be specific for your study.

02. Alternative Hypothesis

The alternative hypothesis is basically another option to the null hypothesis. It involves looking for a significant change or alternative that could lead you to reject the null hypothesis. It’s a different idea compared to the null hypothesis.

When you create a null hypothesis, you’re making an educated guess about whether something is true or if there’s a connection between that thing and another variable. If the null view suggests something is correct, the alternative hypothesis says it’s incorrect. 

For instance, if your null hypothesis is “I’m going to be $1000 richer,” the alternative hypothesis would be “I’m not going to get $1000 or be richer.”

03. Directional Hypothesis

The directional hypothesis predicts the direction of the relationship between independent and dependent variables. They specify whether the effect will be positive or negative.

If you increase your study hours, you will experience a positive association with your exam scores. This hypothesis suggests that as you increase the independent variable (study hours), there will also be an increase in the dependent variable (exam scores).

04. Non-directional Hypothesis

The non-directional hypothesis predicts the existence of a relationship between variables but does not specify the direction of the effect. It suggests that there will be a significant difference or relationship, but it does not predict the nature of that difference.

For example, you will find no notable difference in test scores between students who receive the educational intervention and those who do not. However, once you compare the test scores of the two groups, you will notice an important difference.

05. Simple Hypothesis

A simple hypothesis predicts a relationship between one dependent variable and one independent variable without specifying the nature of that relationship. It’s simple and usually used when we don’t know much about how the two things are connected.

For example, if you adopt effective study habits, you will achieve higher exam scores than those with poor study habits.

06. Complex Hypothesis

A complex hypothesis is an idea that specifies a relationship between multiple independent and dependent variables. It is a more detailed idea than a simple hypothesis.

While a simple view suggests a straightforward cause-and-effect relationship between two things, a complex hypothesis involves many factors and how they’re connected to each other.

For example, when you increase your study time, you tend to achieve higher exam scores. The connection between your study time and exam performance is affected by various factors, including the quality of your sleep, your motivation levels, and the effectiveness of your study techniques.

If you sleep well, stay highly motivated, and use effective study strategies, you may observe a more robust positive correlation between the time you spend studying and your exam scores, unlike those who may lack these factors.

07. Associative Hypothesis

An associative hypothesis proposes a connection between two things without saying that one causes the other. Basically, it suggests that when one thing changes, the other changes too, but it doesn’t claim that one thing is causing the change in the other.

For example, you will likely notice higher exam scores when you increase your study time. You can recognize an association between your study time and exam scores in this scenario.

Your hypothesis acknowledges a relationship between the two variables—your study time and exam scores—without asserting that increased study time directly causes higher exam scores. You need to consider that other factors, like motivation or learning style, could affect the observed association.

08. Causal Hypothesis

A causal hypothesis proposes a cause-and-effect relationship between two variables. It suggests that changes in one variable directly cause changes in another variable.

For example, when you increase your study time, you experience higher exam scores. This hypothesis suggests a direct cause-and-effect relationship, indicating that the more time you spend studying, the higher your exam scores. It assumes that changes in your study time directly influence changes in your exam performance.

09. Empirical Hypothesis

An empirical hypothesis is a statement based on things we can see and measure. It comes from direct observation or experiments and can be tested with real-world evidence. If an experiment proves a theory, it supports the idea and shows it’s not just a guess. This makes the statement more reliable than a wild guess.

For example, if you increase the dosage of a certain medication, you might observe a quicker recovery time for patients. Imagine you’re in charge of a clinical trial. In this trial, patients are given varying dosages of the medication, and you measure and compare their recovery times. This allows you to directly see the effects of different dosages on how fast patients recover.

This way, you can create a research hypothesis: “Increasing the dosage of a certain medication will lead to a faster recovery time for patients.”

10. Statistical Hypothesis

A statistical hypothesis is a statement or assumption about a population parameter that is the subject of an investigation. It serves as the basis for statistical analysis and testing. It is often tested using statistical methods to draw inferences about the larger population.

In a hypothesis test, statistical evidence is collected to either reject the null hypothesis in favor of the alternative hypothesis or fail to reject the null hypothesis due to insufficient evidence.

For example, let’s say you’re testing a new medicine. Your hypothesis could be that the medicine doesn’t really help patients get better. So, you collect data and use statistics to see if your guess is right or if the medicine actually makes a difference.

If the data strongly shows that the medicine does help, you say your guess was wrong, and the medicine does make a difference. But if the proof isn’t strong enough, you can stick with your original guess because you didn’t get enough evidence to change your mind.

How to Develop a Research Hypotheses?

Step 1: identify your research problem or topic..

Define the area of interest or the problem you want to investigate. Make sure it’s clear and well-defined.

Start by asking a question about your chosen topic. Consider the limitations of your research and create a straightforward problem related to your topic. Once you’ve done that, you can develop and test a hypothesis with evidence.

Step 2: Conduct a literature review

Review existing literature related to your research problem. This will help you understand the current state of knowledge in the field, identify gaps, and build a foundation for your hypothesis. Consider the following questions:

• What existing research has been conducted on your chosen topic?
• Are there any gaps or unanswered questions in the current literature?
• How will the existing literature contribute to the foundation of your research?

Step 3: Formulate your research question

Based on your literature review, create a specific and concise research question that addresses your identified problem. Your research question should be clear, focused, and relevant to your field of study.

Step 4: Identify variables

Determine the key variables involved in your research question. Variables are the factors or phenomena that you will study and manipulate to test your hypothesis.

• Independent Variable: The variable you manipulate or control.
• Dependent Variable: The variable you measure to observe the effect of the independent variable.

Step 5: State the Null hypothesis

The null hypothesis is a statement that there is no significant difference or effect. It serves as a baseline for comparison with the alternative hypothesis.

Step 6: Select appropriate methods for testing the hypothesis

Choose research methods that align with your study objectives, such as experiments, surveys, or observational studies. The selected methods enable you to test your research hypothesis effectively.

Creating a research hypothesis usually takes more than one try. Expect to make changes as you collect data. It’s normal to test and say no to a few hypotheses before you find the right answer to your research question.

Testing and Evaluating Hypotheses

Testing hypotheses is a really important part of research. It’s like the practical side of things. Here, real-world evidence will help you determine how different things are connected. Let’s explore the main steps in hypothesis testing:

• State your research hypothesis.

Before testing, clearly articulate your research hypothesis. This involves framing both a null hypothesis, suggesting no significant effect or relationship, and an alternative hypothesis, proposing the expected outcome.

• Collect data strategically.

Plan how you will gather information in a way that fits your study. Make sure your data collection method matches the things you’re studying.

Whether through surveys, observations, or experiments, this step demands precision and adherence to the established methodology. The quality of data collected directly influences the credibility of study outcomes.

• Perform an appropriate statistical test.

Choose a statistical test that aligns with the nature of your data and the hypotheses being tested. Whether it’s a t-test, chi-square test, ANOVA, or regression analysis, selecting the right statistical tool is paramount for accurate and reliable results.

• Decide if your idea was right or wrong.

Following the statistical analysis, evaluate the results in the context of your null hypothesis. You need to decide if you should reject your null hypothesis or not.

• Share what you found.

When discussing what you found in your research, be clear and organized. Say whether your idea was supported or not, and talk about what your results mean. Also, mention any limits to your study and suggest ideas for future research.

The Role of QuestionPro to Develop a Good Research Hypothesis

QuestionPro is a survey and research platform that provides tools for creating, distributing, and analyzing surveys. It plays a crucial role in the research process, especially when you’re in the initial stages of hypothesis development. Here’s how QuestionPro can help you to develop a good research hypothesis:

• Survey design and data collection: You can use the platform to create targeted questions that help you gather relevant data.
• Exploratory research: Through surveys and feedback mechanisms on QuestionPro, you can conduct exploratory research to understand the landscape of a particular subject.
• Literature review and background research: QuestionPro surveys can collect sample population opinions, experiences, and preferences. This data and a thorough literature evaluation can help you generate a well-grounded hypothesis by improving your research knowledge.
• Identifying variables: Using targeted survey questions, you can identify relevant variables related to their research topic.
• Testing assumptions: You can use surveys to informally test certain assumptions or hypotheses before formalizing a research hypothesis.
• Data analysis tools: QuestionPro provides tools for analyzing survey data. You can use these tools to identify the collected data’s patterns, correlations, or trends.
• Refining your hypotheses: As you collect data through QuestionPro, you can adjust your hypotheses based on the real-world responses you receive.

A research hypothesis is like a guide for researchers in science. It’s a well-thought-out idea that has been thoroughly tested. This idea is crucial as researchers can explore different fields, such as medicine, social sciences, and natural sciences. The research hypothesis links theories to real-world evidence and gives researchers a clear path to explore and make discoveries.

QuestionPro Research Suite is a helpful tool for researchers. It makes creating surveys, collecting data, and analyzing information easily. It supports all kinds of research, from exploring new ideas to forming hypotheses. With a focus on using data, it helps researchers do their best work.

Are you interested in learning more about QuestionPro Research Suite? Take advantage of QuestionPro’s free trial to get an initial look at its capabilities and realize the full potential of your research efforts.

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How to Develop a Good Research Hypothesis

The story of a research study begins by asking a question. Researchers all around the globe are asking curious questions and formulating research hypothesis. However, whether the research study provides an effective conclusion depends on how well one develops a good research hypothesis. Research hypothesis examples could help researchers get an idea as to how to write a good research hypothesis.

This blog will help you understand what is a research hypothesis, its characteristics and, how to formulate a research hypothesis

Table of Contents

What is Hypothesis?

Hypothesis is an assumption or an idea proposed for the sake of argument so that it can be tested. It is a precise, testable statement of what the researchers predict will be outcome of the study.  Hypothesis usually involves proposing a relationship between two variables: the independent variable (what the researchers change) and the dependent variable (what the research measures).

What is a Research Hypothesis?

Research hypothesis is a statement that introduces a research question and proposes an expected result. It is an integral part of the scientific method that forms the basis of scientific experiments. Therefore, you need to be careful and thorough when building your research hypothesis. A minor flaw in the construction of your hypothesis could have an adverse effect on your experiment. In research, there is a convention that the hypothesis is written in two forms, the null hypothesis, and the alternative hypothesis (called the experimental hypothesis when the method of investigation is an experiment).

Characteristics of a Good Research Hypothesis

As the hypothesis is specific, there is a testable prediction about what you expect to happen in a study. You may consider drawing hypothesis from previously published research based on the theory.

A good research hypothesis involves more effort than just a guess. In particular, your hypothesis may begin with a question that could be further explored through background research.

To help you formulate a promising research hypothesis, you should ask yourself the following questions:

• Is the language clear and focused?
• What is the relationship between your hypothesis and your research topic?
• Is your hypothesis testable? If yes, then how?
• What are the possible explanations that you might want to explore?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate your variables without hampering the ethical standards?
• Does your research predict the relationship and outcome?
• Is your research simple and concise (avoids wordiness)?
• Is it clear with no ambiguity or assumptions about the readers’ knowledge
• Is your research observable and testable results?
• Is it relevant and specific to the research question or problem?

The questions listed above can be used as a checklist to make sure your hypothesis is based on a solid foundation. Furthermore, it can help you identify weaknesses in your hypothesis and revise it if necessary.

Source: Educational Hub

How to formulate a research hypothesis.

A testable hypothesis is not a simple statement. It is rather an intricate statement that needs to offer a clear introduction to a scientific experiment, its intentions, and the possible outcomes. However, there are some important things to consider when building a compelling hypothesis.

1. State the problem that you are trying to solve.

Make sure that the hypothesis clearly defines the topic and the focus of the experiment.

2. Try to write the hypothesis as an if-then statement.

Follow this template: If a specific action is taken, then a certain outcome is expected.

3. Define the variables

Independent variables are the ones that are manipulated, controlled, or changed. Independent variables are isolated from other factors of the study.

Dependent variables , as the name suggests are dependent on other factors of the study. They are influenced by the change in independent variable.

4. Scrutinize the hypothesis

Evaluate assumptions, predictions, and evidence rigorously to refine your understanding.

Types of Research Hypothesis

The types of research hypothesis are stated below:

1. Simple Hypothesis

It predicts the relationship between a single dependent variable and a single independent variable.

2. Complex Hypothesis

It predicts the relationship between two or more independent and dependent variables.

3. Directional Hypothesis

It specifies the expected direction to be followed to determine the relationship between variables and is derived from theory. Furthermore, it implies the researcher’s intellectual commitment to a particular outcome.

4. Non-directional Hypothesis

It does not predict the exact direction or nature of the relationship between the two variables. The non-directional hypothesis is used when there is no theory involved or when findings contradict previous research.

5. Associative and Causal Hypothesis

The associative hypothesis defines interdependency between variables. A change in one variable results in the change of the other variable. On the other hand, the causal hypothesis proposes an effect on the dependent due to manipulation of the independent variable.

6. Null Hypothesis

Null hypothesis states a negative statement to support the researcher’s findings that there is no relationship between two variables. There will be no changes in the dependent variable due the manipulation of the independent variable. Furthermore, it states results are due to chance and are not significant in terms of supporting the idea being investigated.

7. Alternative Hypothesis

It states that there is a relationship between the two variables of the study and that the results are significant to the research topic. An experimental hypothesis predicts what changes will take place in the dependent variable when the independent variable is manipulated. Also, it states that the results are not due to chance and that they are significant in terms of supporting the theory being investigated.

Research Hypothesis Examples of Independent and Dependent Variables

Research Hypothesis Example 1 The greater number of coal plants in a region (independent variable) increases water pollution (dependent variable). If you change the independent variable (building more coal factories), it will change the dependent variable (amount of water pollution).

Research Hypothesis Example 2 What is the effect of diet or regular soda (independent variable) on blood sugar levels (dependent variable)? If you change the independent variable (the type of soda you consume), it will change the dependent variable (blood sugar levels)

You should not ignore the importance of the above steps. The validity of your experiment and its results rely on a robust testable hypothesis. Developing a strong testable hypothesis has few advantages, it compels us to think intensely and specifically about the outcomes of a study. Consequently, it enables us to understand the implication of the question and the different variables involved in the study. Furthermore, it helps us to make precise predictions based on prior research. Hence, forming a hypothesis would be of great value to the research. Here are some good examples of testable hypotheses.

More importantly, you need to build a robust testable research hypothesis for your scientific experiments. A testable hypothesis is a hypothesis that can be proved or disproved as a result of experimentation.

Importance of a Testable Hypothesis

To devise and perform an experiment using scientific method, you need to make sure that your hypothesis is testable. To be considered testable, some essential criteria must be met:

• There must be a possibility to prove that the hypothesis is true.
• There must be a possibility to prove that the hypothesis is false.
• The results of the hypothesis must be reproducible.

Without these criteria, the hypothesis and the results will be vague. As a result, the experiment will not prove or disprove anything significant.

What are your experiences with building hypotheses for scientific experiments? What challenges did you face? How did you overcome these challenges? Please share your thoughts with us in the comments section.

Frequently Asked Questions

The steps to write a research hypothesis are: 1. Stating the problem: Ensure that the hypothesis defines the research problem 2. Writing a hypothesis as an 'if-then' statement: Include the action and the expected outcome of your study by following a ‘if-then’ structure. 3. Defining the variables: Define the variables as Dependent or Independent based on their dependency to other factors. 4. Scrutinizing the hypothesis: Identify the type of your hypothesis

Hypothesis testing is a statistical tool which is used to make inferences about a population data to draw conclusions for a particular hypothesis.

Hypothesis in statistics is a formal statement about the nature of a population within a structured framework of a statistical model. It is used to test an existing hypothesis by studying a population.

Research hypothesis is a statement that introduces a research question and proposes an expected result. It forms the basis of scientific experiments.

The different types of hypothesis in research are: • Null hypothesis: Null hypothesis is a negative statement to support the researcher’s findings that there is no relationship between two variables. • Alternate hypothesis: Alternate hypothesis predicts the relationship between the two variables of the study. • Directional hypothesis: Directional hypothesis specifies the expected direction to be followed to determine the relationship between variables. • Non-directional hypothesis: Non-directional hypothesis does not predict the exact direction or nature of the relationship between the two variables. • Simple hypothesis: Simple hypothesis predicts the relationship between a single dependent variable and a single independent variable. • Complex hypothesis: Complex hypothesis predicts the relationship between two or more independent and dependent variables. • Associative and casual hypothesis: Associative and casual hypothesis predicts the relationship between two or more independent and dependent variables. • Empirical hypothesis: Empirical hypothesis can be tested via experiments and observation. • Statistical hypothesis: A statistical hypothesis utilizes statistical models to draw conclusions about broader populations.

Wow! You really simplified your explanation that even dummies would find it easy to comprehend. Thank you so much.

Thanks a lot for your valuable guidance.

I enjoy reading the post. Hypotheses are actually an intrinsic part in a study. It bridges the research question and the methodology of the study.

Useful piece!

This is awesome.Wow.

It very interesting to read the topic, can you guide me any specific example of hypothesis process establish throw the Demand and supply of the specific product in market

Nicely explained

It is really a useful for me Kindly give some examples of hypothesis

It was a well explained content ,can you please give me an example with the null and alternative hypothesis illustrated

clear and concise. thanks.

So Good so Amazing

Good to learn

Thanks a lot for explaining to my level of understanding

Explained well and in simple terms. Quick read! Thank you

It awesome. It has really positioned me in my research project

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Research Questions & Hypotheses

Generally, in quantitative studies, reviewers expect hypotheses rather than research questions. However, both research questions and hypotheses serve different purposes and can be beneficial when used together.

Research Questions

Clarify the research’s aim (farrugia et al., 2010).

• Research often begins with an interest in a topic, but a deep understanding of the subject is crucial to formulate an appropriate research question.
• Descriptive: “What factors most influence the academic achievement of senior high school students?”
• Comparative: “What is the performance difference between teaching methods A and B?”
• Relationship-based: “What is the relationship between self-efficacy and academic achievement?”
• Increasing knowledge about a subject can be achieved through systematic literature reviews, in-depth interviews with patients (and proxies), focus groups, and consultations with field experts.
• Some funding bodies, like the Canadian Institute for Health Research, recommend conducting a systematic review or a pilot study before seeking grants for full trials.
• The presence of multiple research questions in a study can complicate the design, statistical analysis, and feasibility.
• It’s advisable to focus on a single primary research question for the study.
• The primary question, clearly stated at the end of a grant proposal’s introduction, usually specifies the study population, intervention, and other relevant factors.
• The FINER criteria underscore aspects that can enhance the chances of a successful research project, including specifying the population of interest, aligning with scientific and public interest, clinical relevance, and contribution to the field, while complying with ethical and national research standards.
• The P ICOT approach is crucial in developing the study’s framework and protocol, influencing inclusion and exclusion criteria and identifying patient groups for inclusion.
• Defining the specific population, intervention, comparator, and outcome helps in selecting the right outcome measurement tool.
• The more precise the population definition and stricter the inclusion and exclusion criteria, the more significant the impact on the interpretation, applicability, and generalizability of the research findings.
• A restricted study population enhances internal validity but may limit the study’s external validity and generalizability to clinical practice.
• A broadly defined study population may better reflect clinical practice but could increase bias and reduce internal validity.
• An inadequately formulated research question can negatively impact study design, potentially leading to ineffective outcomes and affecting publication prospects.

Checklist: Good research questions for social science projects (Panke, 2018)

Research Hypotheses

Present the researcher’s predictions based on specific statements.

• These statements define the research problem or issue and indicate the direction of the researcher’s predictions.
• Formulating the research question and hypothesis from existing data (e.g., a database) can lead to multiple statistical comparisons and potentially spurious findings due to chance.
• The research or clinical hypothesis, derived from the research question, shapes the study’s key elements: sampling strategy, intervention, comparison, and outcome variables.
• Hypotheses can express a single outcome or multiple outcomes.
• After statistical testing, the null hypothesis is either rejected or not rejected based on whether the study’s findings are statistically significant.
• Hypothesis testing helps determine if observed findings are due to true differences and not chance.
• Hypotheses can be 1-sided (specific direction of difference) or 2-sided (presence of a difference without specifying direction).
• 2-sided hypotheses are generally preferred unless there’s a strong justification for a 1-sided hypothesis.
• A solid research hypothesis, informed by a good research question, influences the research design and paves the way for defining clear research objectives.

Types of Research Hypothesis

• In a Y-centered research design, the focus is on the dependent variable (DV) which is specified in the research question. Theories are then used to identify independent variables (IV) and explain their causal relationship with the DV.
• Example: “An increase in teacher-led instructional time (IV) is likely to improve student reading comprehension scores (DV), because extensive guided practice under expert supervision enhances learning retention and skill mastery.”
• Hypothesis Explanation: The dependent variable (student reading comprehension scores) is the focus, and the hypothesis explores how changes in the independent variable (teacher-led instructional time) affect it.
• In X-centered research designs, the independent variable is specified in the research question. Theories are used to determine potential dependent variables and the causal mechanisms at play.
• Example: “Implementing technology-based learning tools (IV) is likely to enhance student engagement in the classroom (DV), because interactive and multimedia content increases student interest and participation.”
• Hypothesis Explanation: The independent variable (technology-based learning tools) is the focus, with the hypothesis exploring its impact on a potential dependent variable (student engagement).
• Probabilistic hypotheses suggest that changes in the independent variable are likely to lead to changes in the dependent variable in a predictable manner, but not with absolute certainty.
• Example: “The more teachers engage in professional development programs (IV), the more their teaching effectiveness (DV) is likely to improve, because continuous training updates pedagogical skills and knowledge.”
• Hypothesis Explanation: This hypothesis implies a probable relationship between the extent of professional development (IV) and teaching effectiveness (DV).
• Deterministic hypotheses state that a specific change in the independent variable will lead to a specific change in the dependent variable, implying a more direct and certain relationship.
• Example: “If the school curriculum changes from traditional lecture-based methods to project-based learning (IV), then student collaboration skills (DV) are expected to improve because project-based learning inherently requires teamwork and peer interaction.”
• Hypothesis Explanation: This hypothesis presumes a direct and definite outcome (improvement in collaboration skills) resulting from a specific change in the teaching method.
• Example : “Students who identify as visual learners will score higher on tests that are presented in a visually rich format compared to tests presented in a text-only format.”
• Explanation : This hypothesis aims to describe the potential difference in test scores between visual learners taking visually rich tests and text-only tests, without implying a direct cause-and-effect relationship.
• Example : “Teaching method A will improve student performance more than method B.”
• Explanation : This hypothesis compares the effectiveness of two different teaching methods, suggesting that one will lead to better student performance than the other. It implies a direct comparison but does not necessarily establish a causal mechanism.
• Example : “Students with higher self-efficacy will show higher levels of academic achievement.”
• Explanation : This hypothesis predicts a relationship between the variable of self-efficacy and academic achievement. Unlike a causal hypothesis, it does not necessarily suggest that one variable causes changes in the other, but rather that they are related in some way.

Tips for developing research questions and hypotheses for research studies

• Perform a systematic literature review (if one has not been done) to increase knowledge and familiarity with the topic and to assist with research development.
• Learn about current trends and technological advances on the topic.
• Seek careful input from experts, mentors, colleagues, and collaborators to refine your research question as this will aid in developing the research question and guide the research study.
• Use the FINER criteria in the development of the research question.
• Ensure that the research question follows PICOT format.
• Develop a research hypothesis from the research question.
• Ensure that the research question and objectives are answerable, feasible, and clinically relevant.

If your research hypotheses are derived from your research questions, particularly when multiple hypotheses address a single question, it’s recommended to use both research questions and hypotheses. However, if this isn’t the case, using hypotheses over research questions is advised. It’s important to note these are general guidelines, not strict rules. If you opt not to use hypotheses, consult with your supervisor for the best approach.

Farrugia, P., Petrisor, B. A., Farrokhyar, F., & Bhandari, M. (2010). Practical tips for surgical research: Research questions, hypotheses and objectives.  Canadian journal of surgery. Journal canadien de chirurgie ,  53 (4), 278–281.

Hulley, S. B., Cummings, S. R., Browner, W. S., Grady, D., & Newman, T. B. (2007). Designing clinical research. Philadelphia.

Panke, D. (2018). Research design & method selection: Making good choices in the social sciences.  Research Design & Method Selection , 1-368.

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Hypothesis is a testable statement that explains what is happening or observed. It proposes the relation between the various participating variables. Hypothesis is also called Theory, Thesis, Guess, Assumption, or Suggestion. Hypothesis creates a structure that guides the search for knowledge.

In this article, we will learn what is hypothesis, its characteristics, types, and examples. We will also learn how hypothesis helps in scientific research.

What is Hypothesis?

A hypothesis is a suggested idea or plan that has little proof, meant to lead to more study. It’s mainly a smart guess or suggested answer to a problem that can be checked through study and trial. In science work, we make guesses called hypotheses to try and figure out what will happen in tests or watching. These are not sure things but rather ideas that can be proved or disproved based on real-life proofs. A good theory is clear and can be tested and found wrong if the proof doesn’t support it.

Hypothesis Meaning

A hypothesis is a proposed statement that is testable and is given for something that happens or observed.

• It is made using what we already know and have seen, and it’s the basis for scientific research.
• A clear guess tells us what we think will happen in an experiment or study.
• It’s a testable clue that can be proven true or wrong with real-life facts and checking it out carefully.
• It usually looks like a “if-then” rule, showing the expected cause and effect relationship between what’s being studied.

Characteristics of Hypothesis

Here are some key characteristics of a hypothesis:

• Testable: An idea (hypothesis) should be made so it can be tested and proven true through doing experiments or watching. It should show a clear connection between things.
• Specific: It needs to be easy and on target, talking about a certain part or connection between things in a study.
• Falsifiable: A good guess should be able to show it’s wrong. This means there must be a chance for proof or seeing something that goes against the guess.
• Logical and Rational: It should be based on things we know now or have seen, giving a reasonable reason that fits with what we already know.
• Predictive: A guess often tells what to expect from an experiment or observation. It gives a guide for what someone might see if the guess is right.
• Concise: It should be short and clear, showing the suggested link or explanation simply without extra confusion.
• Grounded in Research: A guess is usually made from before studies, ideas or watching things. It comes from a deep understanding of what is already known in that area.
• Flexible: A guess helps in the research but it needs to change or fix when new information comes up.
• Relevant: It should be related to the question or problem being studied, helping to direct what the research is about.
• Empirical: Hypotheses come from observations and can be tested using methods based on real-world experiences.

Sources of Hypothesis

Hypotheses can come from different places based on what you’re studying and the kind of research. Here are some common sources from which hypotheses may originate:

• Existing Theories: Often, guesses come from well-known science ideas. These ideas may show connections between things or occurrences that scientists can look into more.
• Observation and Experience: Watching something happen or having personal experiences can lead to guesses. We notice odd things or repeat events in everyday life and experiments. This can make us think of guesses called hypotheses.
• Previous Research: Using old studies or discoveries can help come up with new ideas. Scientists might try to expand or question current findings, making guesses that further study old results.
• Literature Review: Looking at books and research in a subject can help make guesses. Noticing missing parts or mismatches in previous studies might make researchers think up guesses to deal with these spots.
• Problem Statement or Research Question: Often, ideas come from questions or problems in the study. Making clear what needs to be looked into can help create ideas that tackle certain parts of the issue.
• Analogies or Comparisons: Making comparisons between similar things or finding connections from related areas can lead to theories. Understanding from other fields could create new guesses in a different situation.
• Hunches and Speculation: Sometimes, scientists might get a gut feeling or make guesses that help create ideas to test. Though these may not have proof at first, they can be a beginning for looking deeper.
• Technology and Innovations: New technology or tools might make guesses by letting us look at things that were hard to study before.
• Personal Interest and Curiosity: People’s curiosity and personal interests in a topic can help create guesses. Scientists could make guesses based on their own likes or love for a subject.

Types of Hypothesis

Here are some common types of hypotheses:

Simple Hypothesis

Complex hypothesis, directional hypothesis.

• Non-directional Hypothesis

Null Hypothesis (H0)

Alternative hypothesis (h1 or ha), statistical hypothesis, research hypothesis, associative hypothesis, causal hypothesis.

Simple Hypothesis guesses a connection between two things. It says that there is a connection or difference between variables, but it doesn’t tell us which way the relationship goes.

Complex Hypothesis tells us what will happen when more than two things are connected. It looks at how different things interact and may be linked together.

Directional Hypothesis says how one thing is related to another. For example, it guesses that one thing will help or hurt another thing.

Non-Directional Hypothesis

Non-Directional Hypothesis are the one that don’t say how the relationship between things will be. They just say that there is a connection, without telling which way it goes.

Null hypothesis is a statement that says there’s no connection or difference between different things. It implies that any seen impacts are because of luck or random changes in the information.

Alternative Hypothesis is different from the null hypothesis and shows that there’s a big connection or gap between variables. Scientists want to say no to the null hypothesis and choose the alternative one.

Statistical Hypotheis are used in math testing and include making ideas about what groups or bits of them look like. You aim to get information or test certain things using these top-level, common words only.

Research Hypothesis comes from the research question and tells what link is expected between things or factors. It leads the study and chooses where to look more closely.

Associative Hypotheis guesses that there is a link or connection between things without really saying it caused them. It means that when one thing changes, it is connected to another thing changing.

Causal Hypothesis are different from other ideas because they say that one thing causes another. This means there’s a cause and effect relationship between variables involved in the situation. They say that when one thing changes, it directly makes another thing change.

Hypothesis Examples

Following are the examples of hypotheses based on their types:

Simple Hypothesis Example

• Studying more can help you do better on tests.
• Getting more sun makes people have higher amounts of vitamin D.

Complex Hypothesis Example

• How rich you are, how easy it is to get education and healthcare greatly affects the number of years people live.
• A new medicine’s success relies on the amount used, how old a person is who takes it and their genes.

Directional Hypothesis Example

• Drinking more sweet drinks is linked to a higher body weight score.
• Too much stress makes people less productive at work.

Non-directional Hypothesis Example

• Drinking caffeine can affect how well you sleep.
• People often like different kinds of music based on their gender.
• The average test scores of Group A and Group B are not much different.
• There is no connection between using a certain fertilizer and how much it helps crops grow.

Alternative Hypothesis (Ha)

• Patients on Diet A have much different cholesterol levels than those following Diet B.
• Exposure to a certain type of light can change how plants grow compared to normal sunlight.
• The average smarts score of kids in a certain school area is 100.
• The usual time it takes to finish a job using Method A is the same as with Method B.
• Having more kids go to early learning classes helps them do better in school when they get older.
• Using specific ways of talking affects how much customers get involved in marketing activities.
• Regular exercise helps to lower the chances of heart disease.
• Going to school more can help people make more money.
• Playing violent video games makes teens more likely to act aggressively.
• Less clean air directly impacts breathing health in city populations.

Functions of Hypothesis

Hypotheses have many important jobs in the process of scientific research. Here are the key functions of hypotheses:

• Guiding Research: Hypotheses give a clear and exact way for research. They act like guides, showing the predicted connections or results that scientists want to study.
• Formulating Research Questions: Research questions often create guesses. They assist in changing big questions into particular, checkable things. They guide what the study should be focused on.
• Setting Clear Objectives: Hypotheses set the goals of a study by saying what connections between variables should be found. They set the targets that scientists try to reach with their studies.
• Testing Predictions: Theories guess what will happen in experiments or observations. By doing tests in a planned way, scientists can check if what they see matches the guesses made by their ideas.
• Providing Structure: Theories give structure to the study process by arranging thoughts and ideas. They aid scientists in thinking about connections between things and plan experiments to match.
• Focusing Investigations: Hypotheses help scientists focus on certain parts of their study question by clearly saying what they expect links or results to be. This focus makes the study work better.
• Facilitating Communication: Theories help scientists talk to each other effectively. Clearly made guesses help scientists to tell others what they plan, how they will do it and the results expected. This explains things well with colleagues in a wide range of audiences.
• Generating Testable Statements: A good guess can be checked, which means it can be looked at carefully or tested by doing experiments. This feature makes sure that guesses add to the real information used in science knowledge.
• Promoting Objectivity: Guesses give a clear reason for study that helps guide the process while reducing personal bias. They motivate scientists to use facts and data as proofs or disprovals for their proposed answers.
• Driving Scientific Progress: Making, trying out and adjusting ideas is a cycle. Even if a guess is proven right or wrong, the information learned helps to grow knowledge in one specific area.

How Hypothesis help in Scientific Research?

Researchers use hypotheses to put down their thoughts directing how the experiment would take place. Following are the steps that are involved in the scientific method:

• Initiating Investigations: Hypotheses are the beginning of science research. They come from watching, knowing what’s already known or asking questions. This makes scientists make certain explanations that need to be checked with tests.
• Formulating Research Questions: Ideas usually come from bigger questions in study. They help scientists make these questions more exact and testable, guiding the study’s main point.
• Setting Clear Objectives: Hypotheses set the goals of a study by stating what we think will happen between different things. They set the goals that scientists want to reach by doing their studies.
• Designing Experiments and Studies: Assumptions help plan experiments and watchful studies. They assist scientists in knowing what factors to measure, the techniques they will use and gather data for a proposed reason.
• Testing Predictions: Ideas guess what will happen in experiments or observations. By checking these guesses carefully, scientists can see if the seen results match up with what was predicted in each hypothesis.
• Analysis and Interpretation of Data: Hypotheses give us a way to study and make sense of information. Researchers look at what they found and see if it matches the guesses made in their theories. They decide if the proof backs up or disagrees with these suggested reasons why things are happening as expected.
• Encouraging Objectivity: Hypotheses help make things fair by making sure scientists use facts and information to either agree or disagree with their suggested reasons. They lessen personal preferences by needing proof from experience.
• Iterative Process: People either agree or disagree with guesses, but they still help the ongoing process of science. Findings from testing ideas make us ask new questions, improve those ideas and do more tests. It keeps going on in the work of science to keep learning things.

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Summary – Hypothesis

A hypothesis is a testable statement serving as an initial explanation for phenomena, based on observations, theories, or existing knowledge. It acts as a guiding light for scientific research, proposing potential relationships between variables that can be empirically tested through experiments and observations. The hypothesis must be specific, testable, falsifiable, and grounded in prior research or observation, laying out a predictive, if-then scenario that details a cause-and-effect relationship. It originates from various sources including existing theories, observations, previous research, and even personal curiosity, leading to different types, such as simple, complex, directional, non-directional, null, and alternative hypotheses, each serving distinct roles in research methodology. The hypothesis not only guides the research process by shaping objectives and designing experiments but also facilitates objective analysis and interpretation of data, ultimately driving scientific progress through a cycle of testing, validation, and refinement.

FAQs on Hypothesis

What is a hypothesis.

A guess is a possible explanation or forecast that can be checked by doing research and experiments.

What are Components of a Hypothesis?

The components of a Hypothesis are Independent Variable, Dependent Variable, Relationship between Variables, Directionality etc.

What makes a Good Hypothesis?

Testability, Falsifiability, Clarity and Precision, Relevance are some parameters that makes a Good Hypothesis

Can a Hypothesis be Proven True?

You cannot prove conclusively that most hypotheses are true because it’s generally impossible to examine all possible cases for exceptions that would disprove them.

How are Hypotheses Tested?

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data

Can Hypotheses change during Research?

Yes, you can change or improve your ideas based on new information discovered during the research process.

What is the Role of a Hypothesis in Scientific Research?

Hypotheses are used to support scientific research and bring about advancements in knowledge.

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Letter to the Editor From J. Finsterer: “Explaining Long COVID: A Pioneer Cross-Sectional Study Supporting the Endocrine Hypothesis”

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Josef Finsterer, Letter to the Editor From J. Finsterer: “Explaining Long COVID: A Pioneer Cross-Sectional Study Supporting the Endocrine Hypothesis”, Journal of the Endocrine Society , Volume 8, Issue 6, June 2024, bvae086, https://doi.org/10.1210/jendso/bvae086

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We read with interest the article from Ach et al on a cross-sectional study of corticotrophic and somatotrophic functions in 64 patients (G1: fully recovered [n = 32], G2: had long COVID [n = 32]) during a postinfection period of 3 to 15 months after the acute SARS-CoV-2 infection (SC2I) [ 1 ]. G2 had more frequent deficits in the anterior pituitary gland compared to G1 [ 1 ]. Baseline and peak cortisol and growth hormone levels were significantly lower in G2 than in G1 [ 1 ]. It was concluded that the endocrine hypothesis of anterior pituitary insufficiency can be considered as an explanation for long COVID [ 1 ]. The study is impressive, but, in addition to the limitations mentioned in the article, some points require discussion.

The first point is that the dependence of cortisol serum levels on the amount of chronic stress to which the included patients were exposed to during the study period was not reported. Therefore, we should know whether stress has been quantified using, for example, the perceived stress questionnaire (PSQ), the stress and coping inventory (SCI), the perceived stress scale (PSS), or the standard stress scale (SSS), and included in the assessment. We should also know how many of the included patients had returned to work during the study period and how many were still on sick leave.

The second point is that it remained unclear how central nervous system (CNS) impairment in the acute stage of the SC2I, particularly hypophysitis or hypothalamic lesions, could be excluded as a cause of pituitary insufficiency. Hypophysitis is known to be a CNS complication of SC2I [ 2 ]. We should know whether pituitary hormone determination and pituitary magnetic resonance imaging were performed in all enrolled patients in the acute stage of SC2I.

The third point is the discrepancy regarding the exclusion criteria. According to the methods section, any pathology that could interfere with the hormonal assessment was excluded. However, according to Table 1, several patients in G1 and G2 had diabetes. The study cohorts also included several patients with dyspnea, glycemia, and goiter. These discrepancies should be resolved.

A fourth point is that alternative hypotheses to explain long COVID and arguments against the endocrine hypothesis have not been discussed in detail. The strongest argument against the endocrine hypothesis to explain long COVID is that it may only explain some manifestations of long COVID, such as fatigue, exercise intolerance, or brain fog but not many other manifestations. Manifestations of long COVID that cannot be easily explained by the endocrine hypothesis include chronic myocarditis [ 3 ], mast cell activation syndrome (MCAS) [ 4 ], coagulopathy [ 5 ], myositis, dyspnea, arthralgia, multi-inflammatory syndrome (MIS), or dysautonomia. More plausible than the endocrine hypothesis to explain the long COVID syndrome are mechanisms such as the persistence of the virus, hypercoagulopathy, immune dysregulation, autoimmunity, hyperinflammation, or a combination thereof [ 6 ].

In summary, the excellent study has limitations that make the results difficult to interpret. Removing these limitations could strengthen the study's message. Before long COVID can be explained by the endocrine hypothesis alone, several other speculations that claim to explain the disease must be ruled out as causal.

None received.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

J.F. was responsible for the design and conception, discussed available data with coauthors, wrote the first draft, and gave final approval.

Ach T , Ben Haj Slama N , Gorchane A , et al.  Explaining long COVID: a pioneer cross-sectional study supporting the endocrine hypothesis . J Endocr Soc . 2024 ; 8 ( 3 ): bvae003 .

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Misgar RA , Rasool A , Wani AI , Bashir MI . Central diabetes insipidus (Infundibuloneuro hypophysitis): a late complication of COVID-19 infection . J Endocrinol Invest . 2021 ; 44 ( 12 ): 2855 ‐ 2856 .

McMaster MW , Dey S , Fishkin T , Wang A , Frishman WH , Aronow WS . The impact of long COVID-19 on the cardiovascular system . Cardiol Rev . 2024 . doi: 10.1097/CRD.0000000000000654

Sumantri S , Rengganis I . Immunological dysfunction and mast cell activation syndrome in long COVID . Asia Pac Allergy . 2023 ; 13 ( 1 ): 50 ‐ 53 .

Baroni M , Beltrami S , Schiuma G , et al.  In situ endothelial SARS-CoV-2 presence and PROS1 plasma levels alteration in SARS-CoV-2-associated coagulopathies . Life (Basel) . 2024 ; 14 ( 2 ): 237 .

Tziolos NR , Ioannou P , Baliou S , Kofteridis DP . Long COVID-19 pathophysiology: what do we know so far? Microorganisms . 2023 ; 11 ( 10 ): 2458 .

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Interpreting Results from Statistical Hypothesis Testing: Understanding the Appropriate P-value

Eiki tsushima.

1 Graduate School of Health Sciences, Hirosaki University, Japan

Clinical research based on epidemiological study designs requires a good understanding of statistical analysis. This paper discusses the common misconceptions of p-values so that researchers and readers of research papers will be able to properly present and understand the results of null hypothesis significance testing (NHST). The p-values calculated by NHST are categorized as three different types: “significant at p <0.05,” “significant at p <0.01,” or “not significant.” If specified, they may be written as p = 0.124. The 95% confidence interval (CI) of the supplementary statistics is presented regardless of the p-value, and the range of the CI is observed and discussed to determine whether the results are clinically valid. The effect size (ES), which is a measure of the magnitude of the effect, is also referenced and discussed. However, the ES should not be overestimated. It is important to examine the actual descriptive statistics and consider them comprehensively as much as possible. A high detection power of 80% or more indicates that NHST with high accuracy was applied. However, even when it falls below 80%, it is important to consider the limitations of the study, because the results are not completely useless.

C linical research based on epidemiological study designs requires a good understanding of statistical analysis. This is essential not only for the researcher who actually conducts the study and performs the statistical analysis but also for the clinical specialist who reads the research paper.

We, physical therapists, are not statisticians, and so our understanding of statistics is not sufficient. However, because we are increasingly exposed to clinical research, we must avoid misinterpretations. Interpretations of p-values obtained by null hypothesis significance testing (NHST) is a basic knowledge but is often misunderstood. Although it has been a long time since the American Statistical Association (ASA) reported its statement on p-values 1) , it is assumed that the interpretation of p-values is not sufficiently widespread. Andreu et al. 2) surveyed paramedics and respiratory therapists (n = 376) about their knowledge of p-values and reported that 63.1% (237 persons) did not understand them correctly. The lack of training for research and the fact that less than six research papers were read in a year by the subjects were the suggested reasons. In an online survey 3) of 1576 researchers, conducted by Nature , more than 90% of the respondents agreed that robust design of experiments, understanding of appropriate statistics, and mentorship are important for reproducible research. Mentorship was also found to be important.

This paper explains the nature of the p-value based on several reports and other statistics that supplement the lack of information. By addressing common misconceptions of the p-value, the paper aims to help researchers, or readers of research papers, to properly present or understand the results of NHST.

Clinical Significance of p-value

What is a p-value.

The p-value is a decision indicator obtained through NHST. In statistics, the p-value is the probability of rejecting the null hypothesis (H 0 ) when it is true and erroneously adopting the alternative hypothesis (H 1 ). This probability of rejection is called the level of significance, Type I error, or α ( Table 1 ). We conventionally set the level of significance at p = 0.05 or p = 0.01. For example, in the case of a test of the difference of means, we reject the null hypothesis that “the means are equal” if the p-value is p <0.05, which is interpreted as “the means are not equal.” It is erroneous to interpret this as “the means are equal” 4 , 5) .

Determination and errors of hypotheses in NHST

The graph in Figure 1 shows the results of the analysis of the difference between the mean of population A (n A = ∞) (population mean μ A ) and the mean of population B (n B = ∞) (μ B ), when they are equal (μ A = μ B ) and when they are not equal (μ A ≠ μ B ). H 0 is the test of the difference of means at the 5% significance level B for μ A = μ B . An example is given in Figure 1a . It is the 95% (1 − α) that correctly determines that H 0 is true and there is no difference, and conversely, it is the same 95% (1 − β) (power) that correctly determines that H 1 : µ A ≠ µ B is true and there is a difference. This is the result of highly accurate NHST. Because power is unknown for many null hypothesis significance tests, the power in Figure 1b can be as low as 80% (and of course, it can be large). In null hypothesis significance tests with a 5% significance level, even when there is a “significant difference at p <0.05,” information about the Type I error is unknown, except for the fact that it is 5%. The above points are common to all null hypothesis significance tests, not only for tests of differences in means but also for tests of correlation, regression analysis, and analysis of variance.

Probability of “no difference” and “difference” in differences test

Type I error (α) is the probability of mistakenly assuming there is a difference when there is no difference (α = 0.05), and a type II error (β) is the probability of mistakenly assuming there is no difference when there is a difference 1 (H = 0 ). In the case of a, the probabilities of correctly judging that there is a difference and there is no difference, 1 − α = 0.95 and 1 − β = 0.95, are equal. However, in the actual difference test, the probabilities, 1 − α and 1 − β, are often different, as in b. In this figure, the distributions of H 0 and H 1 have the same shape, but in reality, they are not necessarily the same.

The p-value, which is frequently mentioned in many research reports, is a familiar but unusual number. Even if it is said that “there was a significant difference of less than 5%,” it is impossible to understand what the 5% means in the real world.

For example, when the probability that a person does not have a disease after a certain test is as small as p = 0.03, the probability that the person does not have a disease is too small, and therefore, the person is considered to have a disease. However, it is not clear how much it means to be free of a disease at p = 0.03. Although there have been many reports stating that p-values alone do not provide an accurate picture of the facts 1 , 4) , there are still many reports that make judgments based on null hypothesis significance test p-values alone. The p-value should be used as an initial entry point for judgment, and it is necessary to refer to other statistical values afterward to deepen understanding.

Is there no effect when there is no significant difference (p >0.05)?

I have mentioned above that it is wrong to interpret a p-value ≥0.05 as “there is no difference in the means” or “there is no correlation (r = 0).” To be precise, it should be interpreted as “there is insufficient evidence for a difference in the means”; i.e., it is unclear whether there is a difference.

The problem of this misunderstanding has been taken up extensively in Nature 6) , and in 791 papers in five journals, 51% are reported as relevant. Nevertheless, there are still many cases in which a p-value ≥0.05 in NHST is described as “no significant difference” and treated as if there were “no effects at all.” This should be corrected to “there was no significant difference.”

p-value and related statistics

The p-value is a statistic calculated by NHST, but it can be controlled by the data. P-value, effect size (ES), p (α), and power (1 − β) are interrelated, and once three of these are determined, the remaining item can be determined. The relationship between these statistics value is shown in Table 2 . Increasing the sample size increases the power and decreases the p-value in NHST, while increasing the ES decreases the required sample size, increases the power, and decreases the p-value. Thus, intentional manipulation of the sample size and ES results in a corresponding change in the p-value, which can be controlled.

Relationship between sample size, power, ES, and p-value

Considering this mechanism, we can understand the ambiguous nature of p-values. The p-value becomes smaller just by increasing the sample size, and associating it with the ES causes a major problem 2 , 7) .

Notation of p-values

Most statistical analysis software outputs the p-values of NHST results in detail. By referring to the results, you can enter more accurate p-values, for example, p = 0.725 or p = 0.125. However, this is erroneous.

In the case of a test of the difference of means, a significant result should be stated only as “there is a significant difference at p <0.05 (or p <0.01).” If there is no significant difference (i.e., p ≥0.01), the difference is judged to be “not significant” 4) . For example, comparing p = 0.725 and p = 0.125, “p = 0.725 is more likely to have no difference” is also an error. In randomized controlled trials, p-values such as p = 0.852 are sometimes listed to argue that there is no significant difference in background factors between groups, but this may be misleading due to redundant expressions. However, the redundant expression may mislead readers. In a study that examined 30 main nursing journals 8) , it was found that “p = 0.000,” which is an error, was documented in 93.3% of the journals. Documenting p = 0.000 was likely due to the author wanting to strongly emphasize that the results obtained were significant. However, this is misleading to the readers.

In addition, even when the p is close to 0.05, such as p = 0.049 or p = 0.051, it is questionable whether the results should be clearly separated into two 9) . Depending on the nature of the study, a precise statement such as p = 0.210 may be required. In some cases, the p-value should be presented to three decimal places, although it is judged as “there was a significant difference at p <0.05 (or p <0.01)” or “there is no difference” 10) . In any case, as long as we do not understand how much difference the p-value causes in reality, it is a never-ending discussion. Being able to interpret the results comprehensively with supplementary statistics would minimize the problem.

Statistical Values That Supplement the p-value

In medical research papers, NHST is performed and many p-values are described. The display of the confidence interval (CI) and result values is recommended as aid in interpreting p-values 1 , 2 , 5 , 7 , 10 , 11) , and the display of detection power is also helpful.

Confidence interval (%)

A parameter of characteristic value is a statistical value such as the difference of means, correlation coefficient, or regression coefficient in a population. The CI outputs the inferred value of the range containing the parameter. A parameter exists with a confidence of 95% in the 95% CI range. The 95% CI was originally compared with the 5% significance level. The 99% CI should be presented in comparison to a significance level of 1% 7) ; however, the trend is to present only the 95% CI.

To be precise, it does not mean that the range of the 95% CI contains the parameter with a probability of 95% 4 , 12 , 13) . In reality, a parameter is either present (100%) or absent (0%) in the range. The truth of this is a difficult matter to understand, as no conclusion has been reached even in the field of statistics. However, because the range of CI includes a parameter with a high probability, it is possible to estimate the effect more concretely with the CI than with the p-value only.

When the difference is significant at p <0.05, we can estimate the range of the size of the parameter (upper and lower limits) by looking at the CI, and we can consider the extent of the difference by referring to an index such as the minimal clinically important difference (MCID). When the 0.05 difference is not significant, the smaller the upper limit of the CI and the smaller the range of the CI, the smaller the positive effect 12) .

To give a simple example, Clement et al. 14) evaluated Western Ontario and McMaster Universities osteoarthritis index (WOMAC) scores 1 year after total knee arthroplasty (TKA) and calculated the MCID before and after surgery. As a result, the MCID of the total WOMAC score was calculated as 10. Suppose that we conducted a study to evaluate the WOMAC score before and after TKA and obtained the results as shown in Figure 2 ; if the WOMAC score does not improve to a mean value that is higher than 10, there is no clinical efficacy. In cases A to D, a significant change (difference) of p <0.05 was observed in all. A is the mean amount of change (different mean) and is 11, which is certainly higher than the MCID. However, the lower limit of the 95% CI is lower than 10. Moreover, with NHST, even if there is a significant difference, considering the 95% CI, it is difficult to assert that there is a clinically effective difference. In case B, the 95% CI is very narrow and the lower limit exceeds 10. For C, the mean is 20, which is high; however, the lower limit of the 95% CI is below 10, and so the interpretation is the same as that for A. For D, both the mean and lower limit highly exceed 10, and therefore, there is statistical significance and a significant difference even clinically.

Example of mean and 95% CI of change (difference) between pre- and post-TKA surgery

The CI uses two values to represent the range: the upper limit (upper) and the lower limit (lower). Five cases of 95% CIs (A–E) were given. Mean is the mean of the difference between preoperative and postoperative changes. For A–D, all the lower values exceeded 0 (diff. = 0 indicates that the difference is 0); thus, there was a significant change (difference) at p <0.05. For E, the lower limit of the 95% CI was below 0, and therefore, a significant difference could not be detected.

CI, confidence interval; TKA, total knee arthroplasty; WOMAC, Western Ontario and McMaster Universities osteoarthritis index

E is an example of a non-significant difference in NHST. The mean of the differences is 1, and the lower limit of the 95% CI is below 0, which is a negative value (possibly worsened). Empirically, if the WOMAC does not exceed 5, it is considered to be ineffective, and we may conclude that the difference in case E is not statistically significant and clinically equivalent to no effect.

One disadvantage of these CIs is that many of them can only be calculated using parametric methods (NHST for data that follow a normal distribution); most nonparametric methods, such as the Mann–Whitney and Friedman’s tests, cannot be used. The Hodges–Lehmann estimator is used for this purpose, but it is not clear whether it is accurate. Recently, CI of the Mann–Whitney test 15) has been devised. Another disadvantage of the CI is that it is an interval estimate, which makes it difficult to interpret, especially when the sample size is small, because the width of the interval becomes wide. Nevertheless, it should be useful as supplementary information to the p-value.

Effect size

ES is the effect size, and unlike CI, it is not a parameter but the effect of the difference of means, the correlation coefficient or the regression coefficient obtained from the actual sample. N = 10 means the ES of N = 10, N = 100 means the ES of N = 100. Thus, each time you change the sample, you get a different value.

There are two types of ESs, unstandardized effect sizes (U-ESs) and standardized effect size (S-ESs), which have been devised by various researchers. In actuality, 6 15) to 70 16) types have been recognized. In this section, we describe the most frequently used ESs.

The U-ES is the value of the mean or difference of means, regression coefficient, etc. The U-ES varies depending on the size of units and standard deviation of the raw data, making direct comparison with other reports difficult. Therefore, S-ES, which eliminates the effect of units and standard deviation, is often used.

S-ES can be further divided into the d-family and r-family. The d-family includes Cohen’s d, Hedges’ g, and Glass’s delta. The r-family includes ES r, Pearson’s correlation coefficient r, correlation ratio η, phi coefficient, Cramer’s V, and so on. The S-ES can be calculated by nonparametric methods.

The d-family expresses the ES as a numerical value with no upper limit 0, whereas the r-family expresses the ES as a range of 0–1, which has the advantage of being easier to interpret.

The criteria for evaluating the size of ESs are also shown. In Cohen’s d, ≥0.2 are small effects, ≥0.5 are medium effects, and ≥0.8 are large effects 17) . Brydges 18) examined the S-ES based on multiple meta-analyses published in gerontological journals and determined practical values using the quartiles criteria. The actual S-ES was smaller than Cohen’s benchmark and was overestimated.

Because there are many calculation methods for ES, it is necessary to clearly state which ES was used. In addition, more detailed information can be obtained by describing both U-ES and S-ES 5) . This should be considered essential for the odds ratio (OR), which is one of the ESs.

Table 3 shows examples of ORs and 95% CIs of ORs for data with more or fewer people based on the data in a. The ORs are the same for all cases, but some subtables differ greatly when looking at the number of patients. The OR does not change when the number of patients with no disease is increased, as shown in Table 3b – 3d . Although the number of people in a and g are very different, the OR remains constant. However, the range of the 95% CI becomes wider as the number of people decreases. In other words, it is necessary to present the raw data or to provide additional information on the 95% CI, especially when presenting ratio values such as OR.

Observed ORs with 95% CIs when changing data

Power (1 − β)

Power is the probability of obtaining a significant result (to judge correctly) when the alternative hypothesis H 1 is true ( Table 1 ). In the example of the difference of means test, it is the probability of correctly judging that there is a significant difference when there is truly a difference in the population mean. The 95% entered in µ A ≠ µ B in Figure 1a and the 80% in Figure 1b represent the power. Even though there is a difference in the population mean, the probability of incorrectly judging that “there is a significant difference” is called a type II error (β).

There is a trade-off between power and p-value, but in reality, the p-value is fixed at p = 0.05. Therefore, it is described as unrelated in Table 2 . However, power is not determined only by p-value.

A more accurate null hypothesis significance test also has a higher power, because a higher power (1 − β) means a smaller β. The power of a is higher than that of b in Figure 1 , indicating that the null hypothesis significance test is more accurate. Determining the power in this way is useful for evaluating the accuracy of the test.

Although there is theoretical basis for doing so 19) , it is customary to set the power to 0.8 (80%) or 0.95 (95%) 7) . Generally, Type II error (β) is taken as 0.2 and the value is 0.87.

There are two types of power analyses, the a priori method and the post hoc procedure. The a priori method determines the power and ES before the study and determines the sample size required for the null hypothesis significance test in advance. Because the power is fixed at 0.8, the sample size can be obtained if the ES is known in advance. Free software such as G*Power 20) is useful for calculating the detection power, and the ES is generally obtained from previous similar studies or preliminary studies. If multiple null hypothesis significance tests are to be performed, choose the largest sample size for each.

In the post hoc procedure, the NHST is actually performed after the study is completed to evaluate the power of detection. If the power is greater than 80% for all the null hypothesis significance tests, a highly accurate null hypothesis significance test has been applied. In some cases, the power may be low because of insufficient sample size due to dropouts or other factors, and in other cases, the power may exceed 80% because of a large ES.

It does not matter if the power is less than 80%. It is important to consider the reasons for the drop and to be aware of the limits of what can be mentioned.

This paper discussed the meaning of p-values and the need for supplementary statistics with reference to various reports. These are summarized in the following section on how to appropriately present the results of null hypothesis significance tests. In the future, we should be aware of the need to be careful not to let misunderstandings lead us in the wrong direction.

• Judgments based on the p-values calculated by NHST are the following three: “significant at p <0.05,” “significant at p <0.01,” or “not significant.” If specified, “not significant” may be written as p = 0.124.
• If the statistical software outputs the 95% CI, the value should be added. The 95% CI should be presented regardless of the p-value, and the range should be observed and discussed from a professional perspective to determine whether the results are clinically valid.
• ES is one indicator of effect size. It is worth considering, but it should not be overestimated. Whenever possible, actual descriptive statistics should also be taken into consideration.
• In particular, for ESs expressed as ratios such as OR, raw data, descriptive statistics, and 95% CI should be used as additional information to avoid misinterpretation.
• A high detection power of 80% or more indicates that the NHST with high accuracy was applied. However, it is important to consider the limitations of the study, because the results are not completely useless even when the power is below 80%.

The most important thing is to follow a good research design and to have data that are small in bias and well understood. No amount of good statistical analysis can solve this problem.

Conflict of Interest

The author declares no conflicts of interest.

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